##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295042_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3931024 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.167578473191718 33.0 33.0 33.0 27.0 33.0 2 31.16145945687434 33.0 33.0 33.0 27.0 33.0 3 31.19356101616271 33.0 33.0 33.0 27.0 33.0 4 34.80923545620683 37.0 37.0 37.0 33.0 37.0 5 35.081514638425 37.0 37.0 37.0 33.0 37.0 6 35.01956106093476 37.0 37.0 37.0 33.0 37.0 7 34.949731418582026 37.0 37.0 37.0 33.0 37.0 8 34.7931655975644 37.0 37.0 37.0 33.0 37.0 9 34.774775223962 37.0 37.0 37.0 33.0 37.0 10 34.720778860673455 37.0 37.0 37.0 27.0 37.0 11 34.85786705957532 37.0 37.0 37.0 33.0 37.0 12 34.7839450992922 37.0 37.0 37.0 33.0 37.0 13 34.694377088514344 37.0 37.0 37.0 27.0 37.0 14 36.1180379463468 40.0 37.0 40.0 27.0 40.0 15 36.26428584511313 40.0 37.0 40.0 27.0 40.0 16 36.343012914701106 40.0 37.0 40.0 27.0 40.0 17 36.28370317759444 40.0 37.0 40.0 27.0 40.0 18 36.231940837807144 40.0 37.0 40.0 27.0 40.0 19 36.32899264924355 40.0 37.0 40.0 27.0 40.0 20 36.3215635417133 40.0 37.0 40.0 27.0 40.0 21 36.32159050669749 40.0 37.0 40.0 27.0 40.0 22 36.23579479545279 40.0 37.0 40.0 27.0 40.0 23 36.14779507833074 40.0 37.0 40.0 27.0 40.0 24 36.19898886397031 40.0 37.0 40.0 27.0 40.0 25 35.08978856399757 37.0 33.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29356226292234666 1101 2 0.31455313422268816 1101 3 0.2831888725134064 1101 4 0.2978259031874302 1101 5 0.22757982243745545 1101 6 0.3006551672410822 1101 7 0.16559978584277246 1101 8 0.29207439416455827 1101 9 0.3844735912984305 1101 10 0.4083404680458429 1101 11 0.28005699262853767 1101 12 0.4407256169347633 1101 13 0.3272577652388975 1101 14 0.8610790099173329 1101 15 0.7559820201075027 1101 16 0.6884916184734564 1101 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-0.10319138380317838 2214 16 -0.1511723791792079 2214 17 -0.08045303311610752 2214 18 0.041751770547577394 2214 19 -0.604450092028209 2214 20 -0.20835299346888547 2214 21 -0.10682966155530949 2214 22 -0.5005402267562147 2214 23 -1.7004010052817122 2214 24 -1.2169892003403007 2214 25 -1.3743931967800336 2215 1 -0.3372885724165755 2215 2 -0.2049129773102898 2215 3 -1.166524913732502 2215 4 -0.8597425960057947 2215 5 -0.23193656072599111 2215 6 -0.13865821038422155 2215 7 -0.11976272684417211 2215 8 -0.05017283160987063 2215 9 -0.29505847173270183 2215 10 -1.1301436925463406 2215 11 -0.4581616181603323 2215 12 -0.3923037862697285 2215 13 -0.3987619517773098 2215 14 -0.7092393324153861 2215 15 -0.9882243955493664 2215 16 -0.6375879982344728 2215 17 -0.4648238306834642 2215 18 -0.39124221809205295 2215 19 -0.9073681274473984 2215 20 -1.2248783803435828 2215 21 -0.607933026623634 2215 22 -0.31049799892888785 2215 23 -0.6495880889412007 2215 24 -1.6423251440603153 2215 25 -0.6528589283461983 2216 1 -0.5233314204682671 2216 2 -0.3959120821739326 2216 3 -1.3870854282816403 2216 4 -0.742835615797226 2216 5 -0.5037909296690586 2216 6 -0.39520542111358736 2216 7 -0.34090179590236147 2216 8 -0.36705221383139275 2216 9 -0.22570132784800023 2216 10 -0.19777383276607452 2216 11 -0.28007460443289034 2216 12 -0.30570085808165715 2216 13 -1.1676553103539078 2216 14 -0.6398236279871767 2216 15 -1.1643797394698723 2216 16 -0.8360195126371011 2216 17 -1.7396612618825174 2216 18 -0.7631035385857032 2216 19 -0.49020969418020144 2216 20 -0.5413915166447012 2216 21 -0.3887941254626597 2216 22 -0.33477291765868245 2216 23 -0.3111128086361319 2216 24 -0.4578424198702109 2216 25 -0.37485641215935317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5019.0 3 512.0 4 612.0 5 570.0 6 363.0 7 304.0 8 451.0 9 939.0 10 1742.0 11 3347.0 12 5670.0 13 8615.0 14 11842.0 15 15564.0 16 18509.0 17 19786.0 18 20122.0 19 20292.0 20 21272.0 21 22982.0 22 24688.0 23 25732.0 24 27549.0 25 29100.0 26 30924.0 27 34427.0 28 38893.0 29 45414.0 30 55714.0 31 70981.0 32 94564.0 33 134194.0 34 195639.0 35 312962.0 36 573218.0 37 2058512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7198057019179 20.883292799278983 13.397595821509306 22.999305677293812 2 14.985519980999115 21.290546346764092 40.333962958470224 23.38997071376657 3 18.430842888537374 26.54823362327322 29.881259395688677 25.13966409250073 4 11.848287223603572 18.411820447771582 38.31618734115642 31.42370498746842 5 12.302888026067563 39.38960122932489 35.273525297194794 13.033985447412752 6 30.440290108025884 38.6192291380358 17.699026176601322 13.241454577336992 7 25.487121717987797 33.451573275971924 23.343510905634027 17.717794100406252 8 23.784280400605798 37.58793237106842 21.46285305826843 17.16493417005735 9 23.854183927091963 17.532853227965077 22.16253903511567 36.450423809827285 10 14.247053109675182 30.99594503812123 34.2872840423674 20.469717809836183 11 31.333005385648637 26.28247510443774 23.18760051893898 19.196918990974645 12 20.426340534595244 28.581129218702806 31.79206531477854 19.200464931923413 13 26.452636419999838 24.197902815281324 26.537044892764783 22.812415871954055 14 19.751655169126384 24.481285175450605 28.418921884786002 27.34813777063701 15 23.291233255399803 30.706201131714124 24.057246862374722 21.94531875051135 16 20.678201725721777 31.794863821322643 26.878911957194436 20.648022495761143 17 20.05524440820505 30.93289234324329 28.718477054861825 20.29338619368984 18 20.189837635242363 30.22353001752167 29.637214253269445 19.94941809396652 19 20.61658903928371 29.78571987513347 29.909750361245802 19.687940724337018 20 21.765270220533885 29.220943735363413 28.982323440862846 20.031462603239852 21 21.48499975149327 29.5991650173891 28.728958170582665 20.186877060534965 22 20.59315538569807 29.36696735825477 28.95580723214629 21.084070023900864 23 20.167890084579735 29.837462740513903 29.02072358979823 20.973923585108135 24 20.58902334469751 29.708677570597374 28.91763824705339 20.784660837651728 25 21.114992153507774 28.858394013213108 27.87844921907264 22.148164614206483 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9362.0 1 9362.0 2 8167.5 3 6973.0 4 6973.0 5 6973.0 6 9496.0 7 12019.0 8 12019.0 9 12019.0 10 16801.0 11 21583.0 12 21583.0 13 21583.0 14 33422.0 15 45261.0 16 45261.0 17 45261.0 18 68342.5 19 91424.0 20 91424.0 21 91424.0 22 126798.5 23 162173.0 24 162173.0 25 162173.0 26 205821.0 27 249469.0 28 249469.0 29 249469.0 30 292512.5 31 335556.0 32 335556.0 33 335556.0 34 370002.5 35 404449.0 36 404449.0 37 404449.0 38 422292.5 39 440136.0 40 440136.0 41 440136.0 42 439844.0 43 439552.0 44 439552.0 45 439552.0 46 472441.5 47 505331.0 48 505331.0 49 505331.0 50 460959.0 51 416587.0 52 416587.0 53 416587.0 54 362034.0 55 307481.0 56 307481.0 57 307481.0 58 263271.5 59 219062.0 60 219062.0 61 219062.0 62 179085.0 63 139108.0 64 139108.0 65 139108.0 66 106742.5 67 74377.0 68 74377.0 69 74377.0 70 53566.5 71 32756.0 72 32756.0 73 32756.0 74 22753.0 75 12750.0 76 12750.0 77 12750.0 78 8405.5 79 4061.0 80 4061.0 81 4061.0 82 2608.0 83 1155.0 84 1155.0 85 1155.0 86 724.0 87 293.0 88 293.0 89 293.0 90 182.0 91 71.0 92 71.0 93 71.0 94 44.0 95 17.0 96 17.0 97 17.0 98 17.5 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.307731522371779 2 0.1779688956363533 3 0.19541981936513236 4 0.21706812270797632 5 0.2422269617280383 6 0.1912987557440504 7 0.18733032410893447 8 0.21025056066816178 9 0.21047950864711076 10 0.22052778105653895 11 0.19419876347740436 12 0.20017684959440593 13 0.21449881761088208 14 0.20378913992893455 15 0.19320665556862537 16 0.19831982709848628 17 0.19666631391718797 18 0.2005075522306656 19 0.1982943884341586 20 0.1942496408060597 21 0.19305402358265938 22 0.19676806857449866 23 0.19999877894411228 24 0.2152874162050397 25 0.2099198580319021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3931024.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.61412251404124 #Duplication Level Percentage of deduplicated Percentage of total 1 88.83920600578395 68.95177018983063 2 8.730107051941825 13.551591965802162 3 1.401946774805392 3.2643260621373202 4 0.3876466808312711 1.2034742791279884 5 0.17328958889630205 0.6724859691502714 6 0.10266280488686708 0.47808501096746475 7 0.060696929338036054 0.32976572369079044 8 0.044598713090255426 0.2769191985404527 9 0.03521907415265226 0.24601477825035503 >10 0.19620082294160554 2.850223329214559 >50 0.01558549006165462 0.8462687418307354 >100 0.010168269033277035 1.4586132045815807 >500 9.565683975179245E-4 0.46967793865041096 >1k 0.0012864193794559593 2.1130128408292035 >5k 3.2985112293742146E-4 1.7177317724177978 >10k+ 9.895533688122644E-5 1.570038994978334 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 25853 0.6576657888631563 No Hit CCCATGTACTCTGCGTTGATACCAC 23018 0.5855471754942223 No Hit GAGTACATGGGAAGCAGTGGTATCA 12456 0.31686400286541117 No Hit CATGTACTCTGCGTTGATACCACTG 9194 0.23388307982856374 No Hit GCGTTGATACCACTGCTTCCCATGT 7524 0.19140051040136108 No Hit GCTTCCCATGTACTCTGCGTTGATA 7421 0.18878032797561145 No Hit GTATCAACGCAGAGTACATGGGAAG 6991 0.17784170231471494 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6669 0.16965045240120638 No Hit GTATCAACGCAGAGTACTTTTTTTT 6658 0.16937062709360207 No Hit GGTATCAACGCAGAGTACATGGGAA 5946 0.15125829809230368 No Hit TATCAACGCAGAGTACATGGGAAGC 5822 0.14810390371567306 No Hit GGTATCAACGCAGAGTACTTTTTTT 5713 0.14533108930395744 No Hit ACTCTGCGTTGATACCACTGCTTCC 5158 0.13121263060210264 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 4542 0.11554241337626023 No Hit TATCAACGCAGAGTACTTTTTTTTT 4354 0.11075994448265898 No Hit GTGGTATCAACGCAGAGTACATGGG 4080 0.10378975045687842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.543866432766628E-5 0.0 5 0.0 0.0 0.0 2.543866432766628E-5 0.0 6 0.0 0.0 0.0 2.543866432766628E-5 0.0 7 0.0 0.0 0.0 2.543866432766628E-5 0.0 8 0.0 0.0 0.0 2.543866432766628E-5 0.0 9 0.0 0.0 0.0 5.087732865533256E-5 0.0 10 0.0 0.0 0.0 5.087732865533256E-5 0.0 11 0.0 0.0 0.0 1.0175465731066511E-4 0.0 12 2.543866432766628E-5 0.0 0.0 1.0175465731066511E-4 0.0 13 2.543866432766628E-5 0.0 0.0 1.0175465731066511E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 85 5.3301577E-5 11.175908 6 TAGACTG 420 0.0 9.731657 5 TAGACAG 560 0.0 9.50534 5 GTTCTAG 445 0.0 9.403697 1 TACACCG 105 4.4701542E-4 9.052705 5 ATTAGAC 335 0.0 8.795761 3 GTCTTAG 510 0.0 8.764631 1 GTCTCGC 240 1.0004442E-10 8.718011 1 CTAGTAC 285 0.0 8.671317 3 TACACTG 790 0.0 8.6630945 5 TATACTG 820 0.0 8.46207 5 CTACACT 635 0.0 8.383624 4 GTCTTAT 420 0.0 8.378348 1 GTCTTGC 695 0.0 8.347395 1 GTCCTAG 470 0.0 8.296444 1 GTCCTAA 300 1.8189894E-12 8.242483 1 GTTCTAC 335 0.0 8.233019 1 GTTACAC 220 1.4520992E-8 8.208947 3 TTAGACA 475 0.0 8.20555 4 GTCTAAG 280 3.274181E-11 8.151907 1 >>END_MODULE