FastQCFastQC Report
Wed 25 May 2016
SRR1295042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931024
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC256010.6512552454525844No Hit
GTACATGGGAAGCAGTGGTATCAAC246630.6273937783132334No Hit
GTATCAACGCAGAGTACTTTTTTTT163790.41665988302284596No Hit
GGTATCAACGCAGAGTACTTTTTTT151470.3853194485711611No Hit
GAGTACATGGGAAGCAGTGGTATCA136190.34644916947848703No Hit
TATCAACGCAGAGTACTTTTTTTTT115100.29279902641143885No Hit
CATGTACTCTGCGTTGATACCACTG100610.2559384018006504No Hit
GCTTCCCATGTACTCTGCGTTGATA82560.21002161268921277No Hit
ACGCAGAGTACATGGGAAGCAGTGG82170.2090295047804338No Hit
GCGTTGATACCACTGCTTCCCATGT79290.2017031694540659No Hit
GTATCAACGCAGAGTACATGGGAAG72610.18471014168318484No Hit
GGTATCAACGCAGAGTACATGGGAA68370.17392414800825434No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67410.17148203623279837No Hit
TATCAACGCAGAGTACATGGGAAGC65990.16786974589826975No Hit
ACTCTGCGTTGATACCACTGCTTCC59650.15174163271452934No Hit
GTGGTATCAACGCAGAGTACATGGG40890.10401869843582742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG604.0972768E-412.6656486
AACGGCT550.003071436712.0900927
GTAGACG550.003071436712.0900923
CACGGAT600.005881884611.08244217
TACTCCG909.52918E-510.5548425
ACGCCGA750.00265059810.1326487
AGCGCGC750.00265059810.13264810
TACACCG750.00265059810.1326485
CTTACGC750.00265059810.1326483
ACCGCCC900.00111500029.4993588
TATTCCG1401.0607437E-69.4993585
GTGTAGA6050.09.433811
CTTGGAC4350.09.3901693
ATAGGAC2850.09.3327023
GTCTTAG3600.09.2481921
TAGAACG1054.4983783E-49.0470084
CGCCTCC3500.08.95653711
TATACCG850.00744310448.9405725
CCAGGAC5900.08.8553333
GTCCTAT3450.08.8231191
Produced by FastQC (version 0.11.5)