FastQCFastQC Report
Wed 25 May 2016
SRR1295041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300004
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC390410.9079293879726623No Hit
CCCATGTACTCTGCGTTGATACCAC354610.8246736514663707No Hit
GAGTACATGGGAAGCAGTGGTATCA190740.4435809827153649No Hit
CATGTACTCTGCGTTGATACCACTG141660.32944155400785674No Hit
GCGTTGATACCACTGCTTCCCATGT116720.27144160796129496No Hit
GTATCAACGCAGAGTACATGGGAAG103490.24067419472167934No Hit
GCTTCCCATGTACTCTGCGTTGATA102290.2378834996432561No Hit
ACGCAGAGTACATGGGAAGCAGTGG101590.2362555941808426No Hit
TATCAACGCAGAGTACATGGGAAGC90030.20937189825869928No Hit
GGTATCAACGCAGAGTACATGGGAA83960.19525563232034202No Hit
ACTCTGCGTTGATACCACTGCTTCC83530.1942556332505737No Hit
GTATCAACGCAGAGTACTTTTTTTT61680.1434417270309516No Hit
GTGGTATCAACGCAGAGTACATGGG53990.12555802273672304No Hit
GGTATCAACGCAGAGTACTTTTTTT50840.11823244815586217No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN50720.11795337864801986No Hit
CAGTGGTATCAACGCAGAGTACATG46080.10716269101145022No Hit
ATTCCATTCCATTCCATTCCATTCC43330.1007673481233971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG801.282333E-714.2571395
TGTTCCG1054.0862367E-59.9573685
AAGATCG1002.7435418E-49.504765
TACCGTG1002.759656E-49.4992197
TAGGACT4250.09.3934874
GAACGAA1451.715307E-69.16962816
GTCCTAG4150.09.1675251
ACTGTCG950.00182599238.9993658
TAGCGCT850.0074051668.9461784
CCGTATG850.00744423138.9404419
ACCGTGT2607.2759576E-128.7686128
TAGGACA4350.08.7405194
CGCAAAA1856.536902E-88.7335942
TACACTG10050.08.7008755
GTCTTAG4050.08.6893411
ATAAGAC4500.08.6593883
GTGTAGG6050.08.6466431
TTAGGAC2751.8189894E-128.6401863
TAAGACA7300.08.5938454
TTAGACT4100.08.5779674
Produced by FastQC (version 0.11.5)