##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295041_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4300004
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.042150891022427	33.0	33.0	33.0	27.0	33.0
2	30.96543886935919	33.0	33.0	33.0	27.0	33.0
3	30.9997223258397	33.0	33.0	33.0	27.0	33.0
4	34.54999669767749	37.0	37.0	37.0	27.0	37.0
5	34.747605583622715	37.0	37.0	37.0	27.0	37.0
6	34.84385781966714	37.0	37.0	37.0	33.0	37.0
7	34.68983214899335	37.0	37.0	37.0	27.0	37.0
8	34.59976665137986	37.0	37.0	37.0	27.0	37.0
9	34.59554200414697	37.0	37.0	37.0	27.0	37.0
10	34.486213501196744	37.0	37.0	37.0	27.0	37.0
11	34.686437268430446	37.0	37.0	37.0	27.0	37.0
12	34.59033293922517	37.0	37.0	37.0	27.0	37.0
13	34.5071295282516	37.0	37.0	37.0	27.0	37.0
14	35.85888757312784	40.0	37.0	40.0	27.0	40.0
15	36.01300487162337	40.0	37.0	40.0	27.0	40.0
16	36.079264344870374	40.0	37.0	40.0	27.0	40.0
17	36.00362976406534	40.0	37.0	40.0	27.0	40.0
18	35.93274634163131	37.0	37.0	40.0	27.0	40.0
19	36.013955103297576	40.0	37.0	40.0	27.0	40.0
20	36.045507166970076	40.0	37.0	40.0	27.0	40.0
21	36.037323453652604	40.0	37.0	40.0	27.0	40.0
22	35.94644609632921	40.0	37.0	40.0	27.0	40.0
23	35.84748456047948	37.0	37.0	40.0	27.0	40.0
24	35.91583938061453	37.0	37.0	40.0	27.0	40.0
25	34.74379605228274	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.42139066740848463
1101	2	0.29378327828928974
1101	3	0.3435180268919602
1101	4	0.44926354738398544
1101	5	0.29672693481306567
1101	6	0.2308361770080154
1101	7	0.3368291232676768
1101	8	0.43769221197250374
1101	9	0.33576488872049737
1101	10	0.5032159940345196
1101	11	0.33466780526521944
1101	12	0.4696704153851954
1101	13	0.40961359235905803
1101	14	0.7590660241534053
1101	15	0.7038500087908233
1101	16	0.6820704277894905
1101	17	0.6717552648271479
1101	18	0.6601671732625931
1101	19	0.6086853705875868
1101	20	0.63205988500426
1101	21	0.6146005163505635
1101	22	0.6216280969611958
1101	23	0.785085977548718
1101	24	0.7392251626123993
1101	25	0.7992706414510238
1102	1	0.3120762761999458
1102	2	0.3209051360699817
1102	3	0.3262585441979624
1102	4	0.45537575292182453
1102	5	0.2483243436238567
1102	6	0.2614995989093032
1102	7	0.31082614019280896
1102	8	0.3596071689250735
1102	9	0.31576226065163837
1102	10	0.5690700262831285
1102	11	0.345245905551657
1102	12	0.4545583791745855
1102	13	0.3516131075256652
1102	14	0.7813006638149247
1102	15	0.689801045149288
1102	16	0.6319456043690224
1102	17	0.6703023653274371
1102	18	0.679752885984918
1102	19	0.6379365502388481
1102	20	0.636007701485859
1102	21	0.7331560467658775
1102	22	0.707278220420342
1102	23	0.8381969786863337
1102	24	0.6902883632358083
1102	25	0.826423971045827
1103	1	0.3162176832410637
1103	2	0.2512080306212354
1103	3	0.26890936958145417
1103	4	0.3399341925109951
1103	5	0.10004522750831057
1103	6	0.1528671776480124
1103	7	0.15560431621626947
1103	8	0.36161290915031685
1103	9	0.36463969223773773
1103	10	0.4433486526548265
1103	11	0.29740863939554174
1103	12	0.427411078817741
1103	13	0.3684905740761266
1103	14	0.6467083464317795
1103	15	0.6455333245014145
1103	16	0.6037014186203749
1103	17	0.5771828050625345
1103	18	0.5362853999867525
1103	19	0.5200828131982789
1103	20	0.6327368098861825
1103	21	0.5400322495004062
1103	22	0.5952078313362179
1103	23	0.6685501952071391
1103	24	0.6006229418102507
1103	25	0.7218550154397576
1104	1	0.34625679196993886
1104	2	0.33707517296954137
1104	3	0.28287971703586834
1104	4	0.3081975236612493
1104	5	0.21660123212724613
1104	6	0.2843231340093908
1104	7	0.2726282467943264
1104	8	0.4225690263702191
1104	9	0.40117303303949825
1104	10	0.5561920568808105
1104	11	0.3875405433859811
1104	12	0.4604509171549225
1104	13	0.3434016710163661
1104	14	0.6580440610457714
1104	15	0.5886678970198815
1104	16	0.4356698960944385
1104	17	0.5682942943826816
1104	18	0.606308480312606
1104	19	0.5575361355013086
1104	20	0.5052610248391289
1104	21	0.4977534586969199
1104	22	0.5357306789917047
1104	23	0.6557136131060943
1104	24	0.6430607240307893
1104	25	0.7287427764039904
1105	1	0.23987558881457005
1105	2	0.1578022169514739
1105	3	0.14524567408867028
1105	4	0.29664119364593944
1105	5	0.13104386451404793
1105	6	0.12971305413248757
1105	7	0.11384730022250267
1105	8	0.22893643625097582
1105	9	0.1665799060039177
1105	10	0.33314344728107415
1105	11	0.2105454345097968
1105	12	0.32429557051895586
1105	13	0.3361560018182317
1105	14	0.5706610652419855
1105	15	0.3832574253948948
1105	16	0.4199656563568226
1105	17	0.39774930730731484
1105	18	0.3855617486982297
1105	19	0.39190487125462425
1105	20	0.45560829081164655
1105	21	0.3916414630344036
1105	22	0.40361124927918013
1105	23	0.5172663396880708
1105	24	0.4426086005346832
1105	25	0.4146225200495337
1106	1	-0.09380307585659864
1106	2	-0.18381960463942093
1106	3	-0.23376764743916212
1106	4	-0.10966637540628454
1106	5	-0.45141689827860887
1106	6	-0.40262914302857666
1106	7	-0.7597842292486163
1106	8	-0.23582531678426477
1106	9	0.01343079363770272
1106	10	-0.4624174901941913
1106	11	-0.49801421773853605
1106	12	-0.8217139198377552
1106	13	-0.31106985856129654
1106	14	-0.33993169142937774
1106	15	-0.2758707495675381
1106	16	-0.22397164172544848
1106	17	-0.2777701066659688
1106	18	-0.5890231822329497
1106	19	-0.7226708634361358
1106	20	-0.1941237876254931
1106	21	-0.073216541600992
1106	22	0.04659328214361125
1106	23	-0.6386128459161569
1106	24	-5.593740814404669E-4
1106	25	0.06172792550876238
1107	1	-1.0297091408881336
1107	2	-1.0785980263291357
1107	3	-0.7666264974195833
1107	4	-0.8164870810433129
1107	5	-0.9186663588089488
1107	6	-0.9338044191906221
1107	7	-0.9410787659950373
1107	8	-0.9124589308917663
1107	9	-0.953216412843787
1107	10	-0.908352394387002
1107	11	-0.8518283545216221
1107	12	-0.8520304483787697
1107	13	-0.7658380205081201
1107	14	-1.0298378064503808
1107	15	-0.90398928887533
1107	16	-0.9415445460063268
1107	17	-1.0754555943667725
1107	18	-1.1575313656276407
1107	19	-1.1249670462642811
1107	20	-1.3101698707722562
1107	21	-1.1688687077516988
1107	22	-1.118541564442289
1107	23	-0.9472879822267188
1107	24	-0.7982195694926517
1107	25	-0.7527201015141145
1108	1	-0.005225997672045679
1108	2	-0.16239660125913957
1108	3	-0.10239699702952976
1108	4	-0.2781717464505391
1108	5	-0.4726683242066727
1108	6	-0.1650409801832211
1108	7	-0.07677867002264094
1108	8	0.10751747806969547
1108	9	-0.292185644239602
1108	10	-0.5753198232979813
1108	11	-0.1632540824341291
1108	12	0.016985505596252892
1108	13	0.048113118158937596
1108	14	-0.375109729981574
1108	15	-0.0021659699848513014
1108	16	-0.7946621346450371
1108	17	-0.804802731433945
1108	18	-0.6016112576874164
1108	19	-0.08466986601474957
1108	20	-0.9361135384216226
1108	21	-0.24397572376206256
1108	22	-0.42782827753912755
1108	23	-0.11158196896602846
1108	24	-0.45348860027587534
1108	25	-1.1282196749121098
1109	1	0.10991507554841107
1109	2	0.12896104316777013
1109	3	0.2115766017774945
1109	4	0.13769059017396756
1109	5	0.08833400077636355
1109	6	0.16695499343408926
1109	7	0.1662780226713778
1109	8	0.24465843482452954
1109	9	0.24034616458259705
1109	10	0.19910697190869797
1109	11	0.17797382846559628
1109	12	0.20242446424425964
1109	13	0.20645756538485927
1109	14	0.4475579166260957
1109	15	0.3804184605827601
1109	16	0.20176232004419603
1109	17	0.2719077660312692
1109	18	0.35403679833424206
1109	19	0.31627090790944834
1109	20	0.24314062494822508
1109	21	0.31860864908205855
1109	22	0.25292077547742764
1109	23	0.3661314824984103
1109	24	0.42280535010790743
1109	25	0.40213182035103756
1110	1	0.10603730454467808
1110	2	0.052647255218843725
1110	3	0.1752541407886632
1110	4	-0.9222785364053863
1110	5	-0.21645496053330504
1110	6	-0.024794979551245433
1110	7	0.035624475513117204
1110	8	0.1340809906314817
1110	9	0.14723772106724908
1110	10	-0.0875623648180337
1110	11	0.12425405153332036
1110	12	0.10929457158917444
1110	13	0.1533648995982304
1110	14	0.34061957225121375
1110	15	0.19181400706597884
1110	16	0.2158536961309494
1110	17	0.23618192047241138
1110	18	0.3028209010072942
1110	19	0.1949026287232769
1110	20	0.22813318924644932
1110	21	0.15342507196087496
1110	22	-0.3211631774143413
1110	23	0.0820716451933663
1110	24	0.2617976015697039
1110	25	0.33318615583709743
1111	1	-0.06166999782346139
1111	2	-0.33877735415928
1111	3	-0.20165778555006852
1111	4	-0.10986690485044903
1111	5	-0.18119791006525077
1111	6	-0.11743095357826405
1111	7	-0.056441230260645625
1111	8	0.07966099275232352
1111	9	-0.008104501575878942
1111	10	-0.04001851572866144
1111	11	-0.10799247775432264
1111	12	-0.10039828804038109
1111	13	-0.09795083151893635
1111	14	-0.28395171574054956
1111	15	-0.18899434245155788
1111	16	-0.2790896696997365
1111	17	-0.11080453214476194
1111	18	-0.1247820415618861
1111	19	-0.1679323710499716
1111	20	-0.11863751097843789
1111	21	-0.22404428419663702
1111	22	-0.10331945417497224
1111	23	0.02937249773210482
1111	24	0.005191893568387229
1111	25	-0.024905292498374365
1112	1	-0.1407279917435318
1112	2	-0.4620967705629404
1112	3	-0.20403450393703793
1112	4	-0.18289947971420872
1112	5	-0.079484809660336
1112	6	-0.0395305192276183
1112	7	-0.017550376196354023
1112	8	0.05751593648015074
1112	9	-0.021936950270877276
1112	10	0.19583120877081228
1112	11	0.06231163857746225
1112	12	0.11199739616440496
1112	13	0.06849843761864349
1112	14	-0.10117231716185415
1112	15	-0.10685938170546905
1112	16	-0.06992946490670704
1112	17	-0.15299470100601553
1112	18	-0.03144487782416405
1112	19	-0.04592472329235875
1112	20	-0.8119450226945304
1112	21	-1.1681155422068628
1112	22	-1.5889690233093248
1112	23	-0.5034890134881564
1112	24	-0.28528813671481146
1112	25	-1.2334484078373151
1113	1	-0.2125740138053871
1113	2	-0.20869275386761998
1113	3	-0.24335178534219182
1113	4	-0.5263159974142368
1113	5	-0.2830906000865596
1113	6	-0.19559827598366297
1113	7	-0.19552883740735894
1113	8	-0.07909299989263019
1113	9	-0.168863756892506
1113	10	0.04566800143985006
1113	11	-0.13275226867206413
1113	12	-0.06594581239583164
1113	13	-0.225372428686633
1113	14	-0.531403090967494
1113	15	-0.497322158597413
1113	16	-0.41392987018777205
1113	17	-0.44574586017538564
1113	18	-0.7414209259290203
1113	19	-1.047030267157396
1113	20	-1.2452404338528353
1113	21	-1.097859124378843
1113	22	-0.5513433299829984
1113	23	-2.0696832476309552
1113	24	-1.1290658074535642
1113	25	-0.4901579411743313
1114	1	-0.26130791141010334
1114	2	-0.3769249002769115
1114	3	-0.31889957309332573
1114	4	-0.5386945026173251
1114	5	-0.5723639434106929
1114	6	-0.3882861792769532
1114	7	-0.363787811363359
1114	8	-0.12232911176131722
1114	9	-1.0822428081959998
1114	10	-0.2811951858410282
1114	11	-0.3749507938125092
1114	12	-0.3579357381867112
1114	13	-0.40096293010035566
1114	14	-0.7130377820039868
1114	15	-1.2990514549743963
1114	16	-1.174383954133475
1114	17	-1.5254025315105082
1114	18	-1.6875951963775577
1114	19	-1.573236248332634
1114	20	-0.8208420385528541
1114	21	-0.5338270307020636
1114	22	-0.4059404951489185
1114	23	-0.2683095679873091
1114	24	-0.9460481624541117
1114	25	-0.49681438963445146
1115	1	-0.6894827936051549
1115	2	-0.4476045876843564
1115	3	-0.44795758816838926
1115	4	-0.6150644003910912
1115	5	-0.26741594344527186
1115	6	-0.37789785920097785
1115	7	-0.2872157952937897
1115	8	-0.09389458960419006
1115	9	-0.37658984362889925
1115	10	-0.14382566620857773
1115	11	-0.3649470188030719
1115	12	-0.4992341973488905
1115	13	-0.3701649846788655
1115	14	-0.40552103860440525
1115	15	-1.4775103033691792
1115	16	-0.8204453609536557
1115	17	-0.6434374149670958
1115	18	-2.063301089522696
1115	19	-1.0695051846156645
1115	20	-0.8879130538767086
1115	21	-0.8338191701014139
1115	22	-0.6030870010558971
1115	23	-0.35947984812886347
1115	24	-0.40431293566057747
1115	25	-0.4560486502625096
1116	1	-1.4070810798636586
1116	2	-0.7001563489010394
1116	3	-1.604562048837984
1116	4	-0.9259597355486378
1116	5	-0.7990679312418862
1116	6	-0.40360777743114795
1116	7	-1.2170941242503943
1116	8	-2.967838584388872
1116	9	-1.4690790312574435
1116	10	-0.711621644538603
1116	11	-0.5910163611810688
1116	12	-0.6640485646615417
1116	13	-1.6450785887925576
1116	14	-1.48776246666052
1116	15	-1.3550013521272604
1116	16	-0.9800772936211999
1116	17	-1.5793842328511332
1116	18	-2.2891062458024365
1116	19	-1.3238761220352018
1116	20	-0.8335543668288707
1116	21	-0.6077633326994061
1116	22	-0.5031334373963929
1116	23	-0.4462527949609765
1116	24	-0.4544876772640336
1116	25	-0.4704079158667085
1201	1	0.3778199495024346
1201	2	0.29288082848642105
1201	3	0.3641166892892258
1201	4	0.34870226281135075
1201	5	0.26334530327348915
1201	6	0.13068812230899596
1201	7	0.13424449853955167
1201	8	0.34513723768518645
1201	9	0.23713863487223108
1201	10	0.44388442507934656
1201	11	0.3344469205908638
1201	12	0.36698089080446294
1201	13	0.3441597875483353
1201	14	0.751342071979245
1201	15	0.675048518615462
1201	16	0.5545831853792365
1201	17	0.617827630665694
1201	18	0.5156844512484398
1201	19	0.48754114995473685
1201	20	0.6074300217517958
1201	21	0.574974664918777
1201	22	0.6199730049115146
1201	23	0.7119054061173244
1201	24	0.5482813108201654
1201	25	0.6426087725104139
1202	1	0.41434093336487265
1202	2	0.40308588374686494
1202	3	0.34182998955860455
1202	4	0.5050742071229237
1202	5	0.360430849961638
1202	6	0.1190101685817524
1202	7	0.14736463650184106
1202	8	0.3076977220637289
1202	9	0.2893394858075098
1202	10	0.49591383017992285
1202	11	0.26923557143047105
1202	12	0.30106928233539065
1202	13	0.2794527056521048
1202	14	0.7341791103216835
1202	15	0.4945095090235583
1202	16	0.4081462880201556
1202	17	0.5496508476038358
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2210	23	0.6885796727163012
2210	24	0.681952418083462
2210	25	0.6142928072132463
2211	1	0.12333630231676551
2211	2	-0.04040660503189386
2211	3	0.03356403613937786
2211	4	0.035554010736120745
2211	5	0.09801835899833833
2211	6	0.02758191187561465
2211	7	0.12493925117512106
2211	8	0.2299208056586366
2211	9	0.20977288637418923
2211	10	0.3001358316850329
2211	11	0.1143922245239608
2211	12	0.20151477657458372
2211	13	0.06507058447841274
2211	14	-0.012737535440621173
2211	15	0.010750529020469912
2211	16	0.026032056734344167
2211	17	0.11216634074149567
2211	18	0.12467131029146827
2211	19	0.11566044275446785
2211	20	0.10431776844271923
2211	21	0.1508325263726391
2211	22	0.07999806101777551
2211	23	0.20933549515783056
2211	24	0.20243310533022196
2211	25	0.07156285527138806
2212	1	0.19212111424650047
2212	2	0.24582619713563858
2212	3	0.2627667314544375
2212	4	0.13489119874930822
2212	5	0.31533849868072394
2212	6	0.26581608310094396
2212	7	0.20747254187241282
2212	8	0.29462877220809247
2212	9	0.19448549238961022
2212	10	0.33907940283356197
2212	11	0.23097827831067974
2212	12	0.23147878925453114
2212	13	0.1748075659897168
2212	14	0.2067909357554143
2212	15	0.23165494607004433
2212	16	0.14413679335685003
2212	17	0.32646624236771515
2212	18	0.31224954113751124
2212	19	0.28077583682491536
2212	20	0.3394601480684827
2212	21	0.3335954710460385
2212	22	-0.32207600068292663
2212	23	0.2494641410267633
2212	24	0.2226125616170549
2212	25	0.385323518702684
2213	1	-0.008112160021184422
2213	2	-0.6906376785236539
2213	3	-0.07562194728064497
2213	4	0.018706349021023527
2213	5	0.09618850776381294
2213	6	-0.020748986084448973
2213	7	-0.004382436016747704
2213	8	0.08377822924273914
2213	9	-0.2336403902780333
2213	10	-1.381466595730629
2213	11	-0.33568121480511337
2213	12	-0.0581315349576883
2213	13	-0.01414848864864382
2213	14	0.16952703593755558
2213	15	0.047791443230664754
2213	16	-0.006251523314709573
2213	17	-0.17313299158210071
2213	18	0.013010772213334576
2213	19	-0.03708199757063113
2213	20	-0.04155477787818995
2213	21	-0.01862959749838211
2213	22	0.13127156714866572
2213	23	-0.8084576560871071
2213	24	-1.6361983738982389
2213	25	-1.5351147742315945
2214	1	-0.00529220188759183
2214	2	-0.1378866102929166
2214	3	0.014650506941091379
2214	4	-0.1127601877815323
2214	5	-0.018768879604600386
2214	6	-0.1069069932870903
2214	7	0.006468646749851814
2214	8	0.05984962755424306
2214	9	-0.2984474052717374
2214	10	0.05838847701478045
2214	11	-0.10777755884031848
2214	12	-0.059812118226822975
2214	13	-0.07930080820762697
2214	14	-0.05697841734439635
2214	15	-0.05704192676621744
2214	16	-0.2200108482286396
2214	17	-0.062385233953293096
2214	18	-0.024793169260760806
2214	19	-0.7668426688011962
2214	20	-0.3998391724982042
2214	21	-0.30650666526427983
2214	22	-0.47101005866081636
2214	23	-1.6458249580048303
2214	24	-1.1592805722809487
2214	25	-1.5214208607176687
2215	1	-0.5118315205954858
2215	2	-0.22026583973218905
2215	3	-1.1840729448302518
2215	4	-0.8668746779425405
2215	5	-0.3233610975823993
2215	6	-0.23961607920621475
2215	7	-0.27139618262729925
2215	8	-0.12322907905011249
2215	9	-0.24238892786603117
2215	10	-1.40182746433792
2215	11	-0.5043901636129462
2215	12	-0.46894022315974127
2215	13	-0.4136294062243735
2215	14	-0.8020730250183306
2215	15	-0.9907513640094976
2215	16	-0.5982919429147273
2215	17	-0.5284394024489742
2215	18	-0.40105230503409217
2215	19	-1.006084884895536
2215	20	-1.3612225747766615
2215	21	-0.5786090866333993
2215	22	-0.20186343041174126
2215	23	-0.5345202192800684
2215	24	-1.7490245784371936
2215	25	-0.8488510129401945
2216	1	-0.5084104441448396
2216	2	-0.39202536506427066
2216	3	-1.3647382579800436
2216	4	-0.7502001987509601
2216	5	-0.4223804969280138
2216	6	-0.5339530166032418
2216	7	-0.29704530287812503
2216	8	-0.4510225104950223
2216	9	-0.2997568992611548
2216	10	-0.25863651519095754
2216	11	-0.4090989453079388
2216	12	-0.3312371741655227
2216	13	-0.9697273824002224
2216	14	-0.687842136821331
2216	15	-1.1063831102841988
2216	16	-0.8234390992415115
2216	17	-1.7379369798303799
2216	18	-0.6885311456014307
2216	19	-0.5957904545021862
2216	20	-0.4985250632314191
2216	21	-0.2865771506995358
2216	22	-0.3108588231070186
2216	23	-0.23348069967799034
2216	24	-0.3965649086040628
2216	25	-0.34969665437301956
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5620.0
3	578.0
4	759.0
5	652.0
6	396.0
7	324.0
8	671.0
9	1552.0
10	2866.0
11	4776.0
12	7868.0
13	11438.0
14	14841.0
15	19255.0
16	22189.0
17	23292.0
18	24103.0
19	24656.0
20	25879.0
21	27609.0
22	29714.0
23	31218.0
24	33130.0
25	35157.0
26	36756.0
27	40911.0
28	45868.0
29	53906.0
30	65113.0
31	82351.0
32	110164.0
33	155626.0
34	223433.0
35	354329.0
36	644333.0
37	2138671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89498337683133	20.774948516231735	13.05249991368829	23.27756819324865
2	14.649187529515206	21.691390517717977	39.822993798865355	23.83642815390146
3	18.86114196648419	26.634482318298357	29.526553135981672	24.977822579235784
4	12.055340344141968	18.082194781957732	37.79313119127733	32.069333682622975
5	12.419926154184727	39.183812109538735	35.112011298344456	13.284250437932075
6	30.901701875609618	37.95945957911445	17.76439561955421	13.374442925721722
7	25.48549486165916	33.27004919898736	23.21535129568207	18.029104643671413
8	24.437521149764102	37.271594200466666	21.244198089506266	17.046686560262962
9	24.268185448564804	17.36771194789232	21.860782831761018	36.503319771781854
10	14.582045566211974	30.903031252301666	33.895429114305244	20.619494067181122
11	31.47670491604253	26.445222175798616	22.608081886582905	19.469991021575947
12	20.69194134098066	28.640816858234334	31.54717835892424	19.12006344186077
13	26.622504146791165	24.050503103596714	26.183947623994126	23.143045125618
14	20.020251123249874	24.45273028952814	28.060343220417977	27.466675366804004
15	23.959827332094445	30.20975305693264	23.710006466308204	22.120413144664713
16	20.81819444392661	31.576072569730545	26.943688924018776	20.662044062324068
17	20.629758949785014	30.833007875307477	28.519723688466296	20.017509486441217
18	20.740158159228848	29.89787346096529	29.4791604767381	19.88280790306776
19	20.90119372141591	29.755695611991374	29.704149292310046	19.638961374282673
20	21.990000456727834	29.04010722851026	28.677730976120593	20.292161338641318
21	21.41378129172521	29.691853840292488	28.538729153413634	20.355635714568667
22	20.716245971253315	29.3870898811026	28.347765864657415	21.54889828298667
23	20.055108051209846	30.009559084322685	28.722485007711118	21.212847856756355
24	20.707899052960162	30.098936564202955	28.389496208740788	20.803668174096096
25	21.357714093942526	28.669145605205536	27.233404376557836	22.739735924294102
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10602.0
1	10602.0
2	9484.5
3	8367.0
4	8367.0
5	8367.0
6	10909.0
7	13451.0
8	13451.0
9	13451.0
10	18082.0
11	22713.0
12	22713.0
13	22713.0
14	34331.5
15	45950.0
16	45950.0
17	45950.0
18	69525.5
19	93101.0
20	93101.0
21	93101.0
22	128804.5
23	164508.0
24	164508.0
25	164508.0
26	209952.5
27	255397.0
28	255397.0
29	255397.0
30	302333.0
31	349269.0
32	349269.0
33	349269.0
34	387708.0
35	426147.0
36	426147.0
37	426147.0
38	451626.5
39	477106.0
40	477106.0
41	477106.0
42	479915.5
43	482725.0
44	482725.0
45	482725.0
46	539758.0
47	596791.0
48	596791.0
49	596791.0
50	539085.5
51	481380.0
52	481380.0
53	481380.0
54	410289.0
55	339198.0
56	339198.0
57	339198.0
58	287764.0
59	236330.0
60	236330.0
61	236330.0
62	194360.5
63	152391.0
64	152391.0
65	152391.0
66	117362.5
67	82334.0
68	82334.0
69	82334.0
70	59830.0
71	37326.0
72	37326.0
73	37326.0
74	26519.5
75	15713.0
76	15713.0
77	15713.0
78	10758.5
79	5804.0
80	5804.0
81	5804.0
82	4006.0
83	2208.0
84	2208.0
85	2208.0
86	1517.0
87	826.0
88	826.0
89	826.0
90	541.0
91	256.0
92	256.0
93	256.0
94	166.0
95	76.0
96	76.0
97	76.0
98	55.5
99	35.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3073485513036732
2	0.17965099567349238
3	0.19713935149827766
4	0.21839514567893425
5	0.2447904699623535
6	0.19502307439714012
7	0.19183703084927364
8	0.2133951503300927
9	0.21388352196881677
10	0.22399979162810083
11	0.2001160929152624
12	0.20537190197962607
13	0.21713933289364382
14	0.2066044589725963
15	0.19834865269892774
16	0.20065097613862684
17	0.20037190663078452
18	0.20369748493257217
19	0.20274399744744423
20	0.200185860292223
21	0.1984649316605287
22	0.20132539411591246
23	0.20460446083305966
24	0.22039514381847086
25	0.2136974756302552
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4300004.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.40689404381212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.5176498122629	65.11916496544498
2	9.430658899867938	14.034120750516143
3	1.7765209008099168	3.9655620729954335
4	0.513876882217489	1.5294393090688487
5	0.22535083914283732	0.8383828005392622
6	0.13032085902334156	0.5818062209429011
7	0.07729265316073082	0.40257743778669325
8	0.04857855162318109	0.2891663314742313
9	0.037212422460905045	0.2491974697645922
>10	0.2121556352257048	2.887656190151576
>50	0.016352047812513433	0.8235190737712755
>100	0.011165038874784134	1.5814332389152783
>500	0.0010706106693924417	0.546004564631083
>1k	0.0013225187304498312	2.521662454883922
>5k	2.2041978840827594E-4	1.1123224195193384
>10k+	2.519083296094582E-4	3.5179846995944857
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	39041	0.9079293879726623	No Hit
CCCATGTACTCTGCGTTGATACCAC	35461	0.8246736514663707	No Hit
GAGTACATGGGAAGCAGTGGTATCA	19074	0.4435809827153649	No Hit
CATGTACTCTGCGTTGATACCACTG	14166	0.32944155400785674	No Hit
GCGTTGATACCACTGCTTCCCATGT	11672	0.27144160796129496	No Hit
GTATCAACGCAGAGTACATGGGAAG	10349	0.24067419472167934	No Hit
GCTTCCCATGTACTCTGCGTTGATA	10229	0.2378834996432561	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	10159	0.2362555941808426	No Hit
TATCAACGCAGAGTACATGGGAAGC	9003	0.20937189825869928	No Hit
GGTATCAACGCAGAGTACATGGGAA	8396	0.19525563232034202	No Hit
ACTCTGCGTTGATACCACTGCTTCC	8353	0.1942556332505737	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6168	0.1434417270309516	No Hit
GTGGTATCAACGCAGAGTACATGGG	5399	0.12555802273672304	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5084	0.11823244815586217	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5072	0.11795337864801986	No Hit
CAGTGGTATCAACGCAGAGTACATG	4608	0.10716269101145022	No Hit
ATTCCATTCCATTCCATTCCATTCC	4333	0.1007673481233971	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.325579232019319E-5	0.0	0.0	0.0	0.0
9	2.325579232019319E-5	0.0	0.0	2.325579232019319E-5	0.0
10	4.651158464038638E-5	0.0	0.0	4.651158464038638E-5	0.0
11	4.651158464038638E-5	0.0	0.0	1.1627896160096596E-4	0.0
12	4.651158464038638E-5	0.0	0.0	1.1627896160096596E-4	0.0
13	6.976737696057957E-5	0.0	0.0	1.1627896160096596E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	80	1.282333E-7	14.257139	5
TGTTCCG	105	4.0862367E-5	9.957368	5
AAGATCG	100	2.7435418E-4	9.50476	5
TACCGTG	100	2.759656E-4	9.499219	7
TAGGACT	425	0.0	9.393487	4
GAACGAA	145	1.715307E-6	9.169628	16
GTCCTAG	415	0.0	9.167525	1
ACTGTCG	95	0.0018259923	8.999365	8
TAGCGCT	85	0.007405166	8.946178	4
CCGTATG	85	0.0074442313	8.940441	9
ACCGTGT	260	7.2759576E-12	8.768612	8
TAGGACA	435	0.0	8.740519	4
CGCAAAA	185	6.536902E-8	8.733594	2
TACACTG	1005	0.0	8.700875	5
GTCTTAG	405	0.0	8.689341	1
ATAAGAC	450	0.0	8.659388	3
GTGTAGG	605	0.0	8.646643	1
TTAGGAC	275	1.8189894E-12	8.640186	3
TAAGACA	730	0.0	8.593845	4
TTAGACT	410	0.0	8.577967	4
>>END_MODULE