FastQCFastQC Report
Wed 25 May 2016
SRR1295041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300004
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC404510.9407200551441347No Hit
GTACATGGGAAGCAGTGGTATCAAC375960.8743247680699833No Hit
GAGTACATGGGAAGCAGTGGTATCA202620.4712088639917544No Hit
CATGTACTCTGCGTTGATACCACTG159490.3709066317147612No Hit
GTATCAACGCAGAGTACTTTTTTTT154720.35981361877802903No Hit
GGTATCAACGCAGAGTACTTTTTTT137190.31904621484073037No Hit
GCGTTGATACCACTGCTTCCCATGT127690.29695321213654685No Hit
ACGCAGAGTACATGGGAAGCAGTGG126390.2939299591349217No Hit
GCTTCCCATGTACTCTGCGTTGATA114170.26551138091964566No Hit
GTATCAACGCAGAGTACATGGGAAG109870.25551139022196256No Hit
TATCAACGCAGAGTACTTTTTTTTT107960.2510695338888057No Hit
TATCAACGCAGAGTACATGGGAAGC99670.23179048205536554No Hit
ACTCTGCGTTGATACCACTGCTTCC93930.21844165726357465No Hit
GGTATCAACGCAGAGTACATGGGAA92890.21602305486227452No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65200.1516277659276596No Hit
GTGGTATCAACGCAGAGTACATGGG57160.13293010890222426No Hit
CAGTGGTATCAACGCAGAGTACATG56730.13193010983245598No Hit
ACATGGGAAGCAGTGGTATCAACGC53270.12388360568966912No Hit
CATGGGAAGCAGTGGTATCAACGCA48350.11244175586813407No Hit
GCAGTGGTATCAACGCAGAGTACAT45610.10606966877240115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA456.695849E-414.79803851
TGTCCGC500.00150125413.29887810
ACGCCGA750.002650800910.1325957
CCTACAC5350.09.7656433
TAGACTG4800.09.4993095
GGGACGC900.00111509859.4993097
CCGACCA800.00452133739.4991989
TAGGACT4800.09.3014064
CTTAGAC3600.09.2354383
GGACCGT1054.4988302E-49.046966
ACCGTCT850.0074436638.9405258
TGCGCTT850.00744437338.94042110
TTAGACT4050.08.9129314
GGCGCGA1606.757218E-78.90549815
TAGACAG6350.08.6765345
CTAAGAC4450.08.5387043
CCTAGAC3900.08.525023
GTCCTAA3600.08.4560221
CTAGGAC4050.08.443833
TTAGACA5300.08.4239154
Produced by FastQC (version 0.11.5)