FastQCFastQC Report
Wed 25 May 2016
SRR1295040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995519
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC143340.4785147415189154No Hit
CCCATGTACTCTGCGTTGATACCAC120360.4018001554989302No Hit
GAGTACATGGGAAGCAGTGGTATCA70020.23374914330371466No Hit
CATGTACTCTGCGTTGATACCACTG49700.1659144876063213No Hit
GTATCAACGCAGAGTACTTTTTTTT45590.15219399376201587No Hit
GGTATCAACGCAGAGTACTTTTTTT41190.1375053872133677No Hit
GCTTCCCATGTACTCTGCGTTGATA40310.13456766590363806No Hit
GCGTTGATACCACTGCTTCCCATGT39750.13269820688835557No Hit
ACGCAGAGTACATGGGAAGCAGTGG37850.12635539951507568No Hit
GTATCAACGCAGAGTACATGGGAAG36460.12171513517357092No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN34710.11587307575081313No Hit
GGTATCAACGCAGAGTACATGGGAA33180.11076544665548774No Hit
TATCAACGCAGAGTACATGGGAAGC30780.10275347944713421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG755.810216E-815.2088025
TCGGTTT500.001503601613.29589913
GTAGGAC2001.8189894E-1210.4557013
CTAGGAC2350.010.11189653
TAGACAG3950.09.8664695
TAACCCG1201.6898852E-59.5055025
TATTCCG1002.7407668E-49.5055025
ATTAGAC2401.8189894E-129.5051833
CGCAAAA1304.213147E-69.5050242
CGTTCCG900.00111558319.49866210
GTCTCGC2252.5465852E-119.2997191
TAGAACT4850.09.2118254
GACTCCG1252.7597027E-59.1188687
TAGGCGT950.00182793768.9981296
TATACTG6550.08.9975745
TAGGACA3300.08.9297094
GTGTAGG3550.08.8412831
ACTCCGT1304.3765584E-58.7682898
CCGCGCC1304.3860266E-58.76667419
TGTGCGC1201.7673208E-48.70710610
Produced by FastQC (version 0.11.5)