##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295040_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2995519 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.212321137004974 33.0 33.0 33.0 27.0 33.0 2 31.185018689582673 33.0 33.0 33.0 27.0 33.0 3 31.249426226306692 33.0 33.0 33.0 27.0 33.0 4 34.79143914627148 37.0 37.0 37.0 33.0 37.0 5 35.15017664718535 37.0 37.0 37.0 33.0 37.0 6 35.199812119368964 37.0 37.0 37.0 33.0 37.0 7 35.021444364065125 37.0 37.0 37.0 33.0 37.0 8 34.94228712954249 37.0 37.0 37.0 33.0 37.0 9 34.893170766067584 37.0 37.0 37.0 33.0 37.0 10 34.80270363833446 37.0 37.0 37.0 33.0 37.0 11 34.98828183029385 37.0 37.0 37.0 33.0 37.0 12 34.89188684832244 37.0 37.0 37.0 33.0 37.0 13 34.82732541506163 37.0 37.0 37.0 33.0 37.0 14 36.26909026449173 40.0 37.0 40.0 27.0 40.0 15 36.433314227017085 40.0 37.0 40.0 33.0 40.0 16 36.52334336720949 40.0 37.0 40.0 33.0 40.0 17 36.445440339386934 40.0 37.0 40.0 33.0 40.0 18 36.375379692133485 40.0 37.0 40.0 27.0 40.0 19 36.48197357452916 40.0 37.0 40.0 33.0 40.0 20 36.48098776873056 40.0 37.0 40.0 33.0 40.0 21 36.48686654966969 40.0 37.0 40.0 33.0 40.0 22 36.40010595826633 40.0 37.0 40.0 27.0 40.0 23 36.30723490653873 40.0 37.0 40.0 27.0 40.0 24 36.35668343282082 40.0 37.0 40.0 27.0 40.0 25 35.311307322704344 37.0 33.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3753966471277792 1101 2 0.3846904466337193 1101 3 0.34019946397136636 1101 4 0.29789164691673875 1101 5 0.30115921834070036 1101 6 0.3069710007438431 1101 7 0.20300745885413107 1101 8 0.4796557889995938 1101 9 0.4966106273925135 1101 10 0.5465285153338044 1101 11 0.46475247794001007 1101 12 0.5294556658863385 1101 13 0.5079748762696212 1101 14 0.8840870844902895 1101 15 0.7138611866825002 1101 16 0.6330595041627944 1101 17 0.6500365959113168 1101 18 0.6893995260841379 1101 19 0.6762405817851445 1101 20 0.6450651094173168 1101 21 0.6557870371499277 1101 22 0.6997469784868642 1101 23 0.7120709049376615 1101 24 0.7882328715177493 1101 25 0.8335174068601532 1102 1 0.321520020419495 1102 2 0.33406343208616107 1102 3 0.36218940471746564 1102 4 0.4907152768699774 1102 5 0.3107587984011886 1102 6 0.2540615646326927 1102 7 0.2184560038819825 1102 8 0.3862632158037229 1102 9 0.403451091875354 1102 10 0.5995317842066683 1102 11 0.44961098111149056 1102 12 0.48118716110830917 1102 13 0.4598953285491447 1102 14 0.8523243296502514 1102 15 0.6794096262593996 1102 16 0.7133991035143126 1102 17 0.7111259353135111 1102 18 0.7298946238979127 1102 19 0.6382363479116719 1102 20 0.6432694839107711 1102 21 0.6008968930770493 1102 22 0.6802686785343539 1102 23 0.7812500771642448 1102 24 0.76611355274472 1102 25 0.8617824065268778 1103 1 0.24059658271189122 1103 2 0.24319426611818074 1103 3 0.2733902814953524 1103 4 0.278207905482283 1103 5 0.2490202766581291 1103 6 0.12877349963750362 1103 7 0.16362744223361858 1103 8 0.32783977318732127 1103 9 0.3369412124159439 1103 10 0.4269302095931735 1103 11 0.2833132928964801 1103 12 0.42479612707197134 1103 13 0.3414527546863724 1103 14 0.6378768352021069 1103 15 0.6333539159128847 1103 16 0.5803561095887915 1103 17 0.5403589444694106 1103 18 0.6125203694634607 1103 19 0.5924583122580458 1103 20 0.5875449018627279 1103 21 0.5562594999008112 1103 22 0.6283201123306199 1103 23 0.6499320370468809 1103 24 0.6802994733778647 1103 25 0.6857805032198527 1104 1 0.19678335601022567 1104 2 0.09630800232181613 1104 3 0.2399610508865706 1104 4 0.10423702060734286 1104 5 0.12369690951909718 1104 6 0.14471419594838864 1104 7 0.16790593606134507 1104 8 0.27201522195858985 1104 9 0.2588866806973513 1104 10 0.3842145869858342 1104 11 0.26573445745060553 1104 12 0.29049797048723036 1104 13 0.2651209399385408 1104 14 0.5556500088931884 1104 15 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-0.3982011994307726 2216 2 -0.41274393777151275 2216 3 -1.3553764934699544 2216 4 -0.7553986451442398 2216 5 -0.6403884868843477 2216 6 -0.46096613066470127 2216 7 -0.2048664036858341 2216 8 -0.40300361791901906 2216 9 -0.3133787421233336 2216 10 -0.3361809262248556 2216 11 -0.37124801525227014 2216 12 -0.3796033507885781 2216 13 -0.992201768531956 2216 14 -0.7053454405517599 2216 15 -1.0735978891330902 2216 16 -0.8223645334498286 2216 17 -1.7431312777692796 2216 18 -0.8704895091844094 2216 19 -0.6677178480404109 2216 20 -0.5304573794090217 2216 21 -0.38983258688634237 2216 22 -0.4696608180494124 2216 23 -0.3194833767733485 2216 24 -0.525801487309316 2216 25 -0.4322068509578827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3854.0 3 391.0 4 489.0 5 431.0 6 315.0 7 240.0 8 335.0 9 718.0 10 1308.0 11 2408.0 12 4013.0 13 6091.0 14 8270.0 15 10766.0 16 12454.0 17 13094.0 18 13669.0 19 14132.0 20 14613.0 21 15875.0 22 17106.0 23 18073.0 24 19415.0 25 21019.0 26 22573.0 27 25553.0 28 28573.0 29 33684.0 30 41316.0 31 52597.0 32 71042.0 33 101598.0 34 148565.0 35 239577.0 36 442674.0 37 1588688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78018850801658 21.174032695886993 12.845047160491676 23.20073163560475 2 15.339577631957823 21.495809060509185 40.005404427416636 23.159208880116356 3 18.41097912029762 26.29818283503958 30.19291531113506 25.09792273352774 4 12.049904683139188 18.02288273784551 38.59673774781882 31.33047483119648 5 12.250145823914318 39.237592752543094 35.593885669366074 12.91837575417652 6 30.49807957512837 39.053912713068826 17.57846849670352 12.869539215099293 7 25.753778550469477 33.344359575455115 23.38876667858482 17.513095195490592 8 23.744886792661134 37.43093594569286 21.474182798479664 17.34999446316634 9 23.818273120248744 17.165521553629063 22.30767403604923 36.70853129007296 10 13.964651821377405 30.77086251188611 34.77808016777403 20.486405498962455 11 31.613006164437863 25.95526375558735 23.37174580800497 19.05998427196982 12 20.13906613736879 28.347955705702443 32.0820547747702 19.43092338215858 13 26.35250695223234 24.226890351452408 26.833241439854827 22.587361256460422 14 19.68996137898274 24.259384706478595 28.68352342768637 27.367130486852293 15 22.88155662472639 31.070948820730738 24.2859694519221 21.761525102620777 16 20.759713088857424 31.84850160411601 26.689908034696135 20.701877272330428 17 19.658796092498758 30.958825662344474 28.94118544307269 20.44119280208407 18 19.835341609961926 30.4032966466876 29.88353757298136 19.877824170369117 19 20.32117796250995 29.710985327317925 30.125244486161755 19.84259222401037 20 21.507244476496425 29.376021043702345 29.214560668560352 19.902173811240882 21 21.355031806483854 29.722053677329928 28.731399946883272 20.191514569302946 22 20.407912733675612 29.478642516409597 29.351199055647303 20.762245694267488 23 20.178106459787895 29.885718032225345 29.22275282928487 20.71342267870189 24 20.44270245222765 29.6501573313393 29.228164735363837 20.678975481069216 25 21.034326443288016 29.123530700763943 28.296123074040807 21.546019781907233 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6305.0 1 6305.0 2 5228.5 3 4152.0 4 4152.0 5 4152.0 6 6032.0 7 7912.0 8 7912.0 9 7912.0 10 12025.0 11 16138.0 12 16138.0 13 16138.0 14 25698.5 15 35259.0 16 35259.0 17 35259.0 18 54152.0 19 73045.0 20 73045.0 21 73045.0 22 100371.0 23 127697.0 24 127697.0 25 127697.0 26 161298.0 27 194899.0 28 194899.0 29 194899.0 30 229146.0 31 263393.0 32 263393.0 33 263393.0 34 289389.5 35 315386.0 36 315386.0 37 315386.0 38 329365.5 39 343345.0 40 343345.0 41 343345.0 42 339272.5 43 335200.0 44 335200.0 45 335200.0 46 348123.0 47 361046.0 48 361046.0 49 361046.0 50 333276.0 51 305506.0 52 305506.0 53 305506.0 54 270014.5 55 234523.0 56 234523.0 57 234523.0 58 201808.0 59 169093.0 60 169093.0 61 169093.0 62 138682.5 63 108272.0 64 108272.0 65 108272.0 66 81942.5 67 55613.0 68 55613.0 69 55613.0 70 40101.5 71 24590.0 72 24590.0 73 24590.0 74 16949.5 75 9309.0 76 9309.0 77 9309.0 78 6267.0 79 3225.0 80 3225.0 81 3225.0 82 2157.5 83 1090.0 84 1090.0 85 1090.0 86 718.5 87 347.0 88 347.0 89 347.0 90 234.5 91 122.0 92 122.0 93 122.0 94 75.5 95 29.0 96 29.0 97 29.0 98 26.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3115653748148485 2 0.17953483186052233 3 0.19752837488261635 4 0.21835948962433557 5 0.2445652990349919 6 0.19415667201576756 7 0.18968332365777016 8 0.2151880859376956 9 0.21498778675748675 10 0.2249025961778243 11 0.1986967867671679 12 0.20530665971405956 13 0.21869332159135027 14 0.20744318430295383 15 0.19599274783434856 16 0.201033610536271 17 0.19986519865171945 18 0.20220202242082255 19 0.20317013512516527 20 0.197995739636437 21 0.19619304701455742 22 0.19889708594737673 23 0.2026693871746432 24 0.22046263101652835 25 0.21445365561026322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2995519.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.59167977875084 #Duplication Level Percentage of deduplicated Percentage of total 1 91.56265998096528 75.6231389283853 2 6.805413123551658 11.241410029249742 3 0.8834026716832586 2.1888513172607 4 0.24669446871579032 0.814996422534545 5 0.11840659374352817 0.48896997370790607 6 0.08335244683143118 0.4130531158486152 7 0.053502349878406574 0.3093194263997632 8 0.03836785543321676 0.2535092503790116 9 0.029786141730319446 0.22140787316715285 >10 0.15389428573996647 2.3201111183446863 >50 0.013649182255141086 0.778413574047549 >100 0.0086371470804569 1.2632978849987968 >500 8.12273597490617E-4 0.4950017307940346 >1k 0.0012996377545138951 2.4691049924148016 >5k 4.061367982855922E-5 0.2348717297733761 >10k+ 8.122735965711845E-5 0.884542632694077 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 14334 0.4785147415189154 No Hit CCCATGTACTCTGCGTTGATACCAC 12036 0.4018001554989302 No Hit GAGTACATGGGAAGCAGTGGTATCA 7002 0.23374914330371466 No Hit CATGTACTCTGCGTTGATACCACTG 4970 0.1659144876063213 No Hit GTATCAACGCAGAGTACTTTTTTTT 4559 0.15219399376201587 No Hit GGTATCAACGCAGAGTACTTTTTTT 4119 0.1375053872133677 No Hit GCTTCCCATGTACTCTGCGTTGATA 4031 0.13456766590363806 No Hit GCGTTGATACCACTGCTTCCCATGT 3975 0.13269820688835557 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3785 0.12635539951507568 No Hit GTATCAACGCAGAGTACATGGGAAG 3646 0.12171513517357092 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 3471 0.11587307575081313 No Hit GGTATCAACGCAGAGTACATGGGAA 3318 0.11076544665548774 No Hit TATCAACGCAGAGTACATGGGAAGC 3078 0.10275347944713421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.33831967014731E-5 0.0 2 0.0 0.0 0.0 3.33831967014731E-5 0.0 3 0.0 0.0 0.0 3.33831967014731E-5 0.0 4 0.0 0.0 0.0 6.67663934029462E-5 0.0 5 0.0 0.0 0.0 6.67663934029462E-5 0.0 6 0.0 0.0 0.0 6.67663934029462E-5 0.0 7 0.0 0.0 0.0 6.67663934029462E-5 0.0 8 0.0 0.0 0.0 6.67663934029462E-5 0.0 9 0.0 0.0 0.0 2.336823769103117E-4 0.0 10 0.0 0.0 0.0 2.336823769103117E-4 0.0 11 0.0 0.0 0.0 2.336823769103117E-4 0.0 12 0.0 0.0 0.0 2.336823769103117E-4 0.0 13 0.0 0.0 0.0 2.670655736117848E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCCG 75 5.810216E-8 15.208802 5 TCGGTTT 50 0.0015036016 13.295899 13 GTAGGAC 200 1.8189894E-12 10.455701 3 CTAGGAC 235 0.0 10.1118965 3 TAGACAG 395 0.0 9.866469 5 TAACCCG 120 1.6898852E-5 9.505502 5 TATTCCG 100 2.7407668E-4 9.505502 5 ATTAGAC 240 1.8189894E-12 9.505183 3 CGCAAAA 130 4.213147E-6 9.505024 2 CGTTCCG 90 0.0011155831 9.498662 10 GTCTCGC 225 2.5465852E-11 9.299719 1 TAGAACT 485 0.0 9.211825 4 GACTCCG 125 2.7597027E-5 9.118868 7 TAGGCGT 95 0.0018279376 8.998129 6 TATACTG 655 0.0 8.997574 5 TAGGACA 330 0.0 8.929709 4 GTGTAGG 355 0.0 8.841283 1 ACTCCGT 130 4.3765584E-5 8.768289 8 CCGCGCC 130 4.3860266E-5 8.766674 19 TGTGCGC 120 1.7673208E-4 8.707106 10 >>END_MODULE