FastQCFastQC Report
Wed 25 May 2016
SRR1295040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995519
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC139300.4650279300515203No Hit
GTACATGGGAAGCAGTGGTATCAAC134030.44743498538984394No Hit
GTATCAACGCAGAGTACTTTTTTTT107130.35763418626288135No Hit
GGTATCAACGCAGAGTACTTTTTTT102540.34231129897690515No Hit
GAGTACATGGGAAGCAGTGGTATCA72420.24176111051206817No Hit
TATCAACGCAGAGTACTTTTTTTTT71500.23868985641553267No Hit
CATGTACTCTGCGTTGATACCACTG58000.193622540868544No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47330.15800266998807216No Hit
GCTTCCCATGTACTCTGCGTTGATA46090.15386315359708952No Hit
ACGCAGAGTACATGGGAAGCAGTGG44960.15009085236982306No Hit
GCGTTGATACCACTGCTTCCCATGT42740.14267978270209603No Hit
GTATCAACGCAGAGTACATGGGAAG39040.13032799992255098No Hit
GGTATCAACGCAGAGTACATGGGAA36170.12074702246922821No Hit
TATCAACGCAGAGTACATGGGAAGC33070.11039823149177155No Hit
ACTCTGCGTTGATACCACTGCTTCC30920.10322084420095483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG551.1296923E-515.5444575
GTACCGG551.9624527E-413.8170656
CTGTCGT600.00588208411.08225259
ACTGTCG1054.1113122E-59.9515778
GTATTAG4500.09.7260731
TATTCCG1106.84518E-59.4993915
AGATCGA800.0045205279.4992328
GTGCGCC800.00452114979.49907311
TAGACAG5050.09.4053375
TCTGCAC5200.09.316713
TAGACTG4450.09.1791875
CCGTGCT1151.1123835E-49.086079
TTAGACT3050.09.03220754
CTTACAC4000.09.0244223
AGAGGCG950.00182555068.9994235
TAGGACT4050.08.9130094
GTGTAGG3000.08.8803281
AGCACCG1401.0866328E-58.8208635
GTCTAGC2054.0308805E-98.8184441
TATACTG6100.08.7207525
Produced by FastQC (version 0.11.5)