FastQCFastQC Report
Wed 25 May 2016
SRR1295039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670476
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC307651.1520418082768764No Hit
CCCATGTACTCTGCGTTGATACCAC273311.0234505009593795No Hit
GAGTACATGGGAAGCAGTGGTATCA147160.551062806780514No Hit
CATGTACTCTGCGTTGATACCACTG107540.4026997434165295No Hit
GCTTCCCATGTACTCTGCGTTGATA89110.3336858297921419No Hit
GCGTTGATACCACTGCTTCCCATGT83390.312266427408447No Hit
GTATCAACGCAGAGTACATGGGAAG76630.2869525882277167No Hit
ACGCAGAGTACATGGGAAGCAGTGG74300.2782275519420508No Hit
TATCAACGCAGAGTACATGGGAAGC66740.24991799214821625No Hit
GGTATCAACGCAGAGTACATGGGAA61430.23003389657873727No Hit
ACTCTGCGTTGATACCACTGCTTCC57930.2169276188964065No Hit
GTATCAACGCAGAGTACTTTTTTTT38760.14514266370489753No Hit
GTGGTATCAACGCAGAGTACATGGG35300.13218617205322197No Hit
CAGTGGTATCAACGCAGAGTACATG33150.12413517290550448No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN31240.11698288994171827No Hit
ACATGGGAAGCAGTGGTATCAACGC30160.11293866711402761No Hit
GGTATCAACGCAGAGTACTTTTTTT30150.11290122060636382No Hit
GCAGTGGTATCAACGCAGAGTACAT29120.10904423031699217No Hit
GGGAAGCAGTGGTATCAACGCAGAG28280.10589872367323279No Hit
CATGGGAAGCAGTGGTATCAACGCA27900.10447575638200829No Hit
ATTCCATTCCATTCCATTCCATTCC27690.10368937972106845No Hit
ATACCACTGCTTCCCATGTACTCTG26950.10091833815394709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA907.4326053E-611.6157952
TAGACAG4000.011.4056695
TAGGACT3150.010.86335854
CTTACAC4500.010.3486173
GACTGTC1701.5606929E-910.0587237
GTCTTAC2750.010.028251
GTAGGAC1752.557499E-99.7753693
GTCTTGG3300.09.5095471
GTCCTAT1606.5379936E-89.5095461
CTAGACT3300.09.5054384
GGACCTT1304.246571E-69.4990146
CCTATAC2451.8189894E-129.3098763
TATACTG6550.09.2870585
TACACTG5950.09.2651095
GTCTTAG3000.09.1925611
TTAGGAC2304.1836756E-119.0906213
GTATTAT6000.09.0340691
TATTCCG950.00181611459.0044755
CGGGTTC1901.0633812E-88.99754618
CTAGGAC2252.6011548E-108.8702433
Produced by FastQC (version 0.11.5)