##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295039_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2670476
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.002575570797116	33.0	33.0	33.0	27.0	33.0
2	30.91896500848538	33.0	33.0	33.0	27.0	33.0
3	31.031637430929916	33.0	33.0	33.0	27.0	33.0
4	34.5501622182712	37.0	37.0	37.0	27.0	37.0
5	34.84046140088883	37.0	37.0	37.0	33.0	37.0
6	34.686892524029425	37.0	37.0	37.0	27.0	37.0
7	34.62591500541477	37.0	37.0	37.0	27.0	37.0
8	34.543205031612345	37.0	37.0	37.0	27.0	37.0
9	34.51026596007603	37.0	37.0	37.0	27.0	37.0
10	34.44533221792669	37.0	37.0	37.0	27.0	37.0
11	34.61156999725892	37.0	37.0	37.0	27.0	37.0
12	34.519310040606996	37.0	37.0	37.0	27.0	37.0
13	34.42064972686517	37.0	37.0	37.0	27.0	37.0
14	35.790089482174714	40.0	37.0	40.0	27.0	40.0
15	35.92077404927062	40.0	37.0	40.0	27.0	40.0
16	36.000229547091976	40.0	37.0	40.0	27.0	40.0
17	35.9266168278614	40.0	37.0	40.0	27.0	40.0
18	35.86130487598466	37.0	37.0	40.0	27.0	40.0
19	35.935105951148785	40.0	37.0	40.0	27.0	40.0
20	35.95244967563835	40.0	37.0	40.0	27.0	40.0
21	35.93802415749102	40.0	37.0	40.0	27.0	40.0
22	35.85002111983032	40.0	37.0	40.0	27.0	40.0
23	35.76115269337751	37.0	37.0	40.0	27.0	40.0
24	35.82839014467833	37.0	37.0	40.0	27.0	40.0
25	34.645453469718504	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.434873219109992
1101	2	0.4719310578335012
1101	3	0.4360285849595904
1101	4	0.5351858735689348
1101	5	0.40929090862957196
1101	6	0.4140168063145353
1101	7	0.4708228259529861
1101	8	0.48676309073027824
1101	9	0.39808326237185554
1101	10	0.6397805510822892
1101	11	0.4242706415004278
1101	12	0.5123924241418649
1101	13	0.43197840309483126
1101	14	0.9726588306187196
1101	15	0.867922786697676
1101	16	0.7071551633452344
1101	17	0.6901526410217969
1101	18	0.7346328230069119
1101	19	0.6821800227342081
1101	20	0.6338731044618271
1101	21	0.6932186027883418
1101	22	0.7071471743768711
1101	23	0.8224464279815535
1101	24	0.7955932242036639
1101	25	0.9078717369272908
1102	1	0.24522230679372115
1102	2	0.26514747952635176
1102	3	0.2533121632667381
1102	4	0.2743523712881455
1102	5	0.08780193245116408
1102	6	0.002161129679969065
1102	7	0.08336426031689825
1102	8	0.31004223416666576
1102	9	0.2379084017535078
1102	10	0.39063863521812436
1102	11	0.3002169162089956
1102	12	0.3046618108625978
1102	13	0.2025108410066423
1102	14	0.5793793070505515
1102	15	0.5838606985071024
1102	16	0.6068725987228305
1102	17	0.5020299851677663
1102	18	0.5877975467778214
1102	19	0.4771157804635493
1102	20	0.5250991561597473
1102	21	0.5732730883433348
1102	22	0.5663705651523401
1102	23	0.652526128944551
1102	24	0.5688237107723424
1102	25	0.6297716355588108
1103	1	0.3222410344869502
1103	2	0.27119654676109306
1103	3	0.21404587781850992
1103	4	0.24207228809991221
1103	5	0.131095275387473
1103	6	0.20540801059420488
1103	7	0.2739723034349453
1103	8	0.39048646719208335
1103	9	0.29567594711930667
1103	10	0.48151456451846286
1103	11	0.2822268495123694
1103	12	0.4631743350649842
1103	13	0.4338772585315098
1103	14	0.6297447972770982
1103	15	0.6351500820447811
1103	16	0.6537034340593451
1103	17	0.4447687146719588
1103	18	0.5744392425142522
1103	19	0.4487263651079303
1103	20	0.5282416040885991
1103	21	0.5048116607629609
1103	22	0.6230263109780196
1103	23	0.6703107599049147
1103	24	0.644716451374606
1103	25	0.6801721847998934
1104	1	0.38568965354641804
1104	2	0.24059191874417252
1104	3	0.3531352035214219
1104	4	0.3724529798142697
1104	5	0.2669499656089158
1104	6	0.17803801805874997
1104	7	0.2615313977370164
1104	8	0.382524287545408
1104	9	0.34220493442958144
1104	10	0.5586481798456617
1104	11	0.352144739534765
1104	12	0.45396806484851027
1104	13	0.41073717393334874
1104	14	0.7083713212880411
1104	15	0.6309201741074801
1104	16	0.4860000252511796
1104	17	0.5492723225346055
1104	18	0.5998186901380365
1104	19	0.42608391673768864
1104	20	0.6143326715183122
1104	21	0.6543513472521383
1104	22	0.5745067787560671
1104	23	0.6143266842642063
1104	24	0.6009244633178739
1104	25	0.7421278951756065
1105	1	0.27973296017040994
1105	2	0.15418999741426376
1105	3	0.1823954160323389
1105	4	0.16284123731739442
1105	5	0.14821291849393248
1105	6	0.06705163985337492
1105	7	0.06140204913424441
1105	8	0.1563786887574068
1105	9	0.17641248555311506
1105	10	0.2420012662487494
1105	11	0.15139024692582836
1105	12	0.2696458150173271
1105	13	0.251119586818632
1105	14	0.4350712843795108
1105	15	0.3808925770799192
1105	16	0.28693013251909605
1105	17	0.258510224252376
1105	18	0.25788559735956085
1105	19	0.1639405036220012
1105	20	0.22243414022015173
1105	21	0.22235855346652045
1105	22	0.24720173664567824
1105	23	0.32501208766095857
1105	24	0.3687575275328925
1105	25	0.22446791448585657
1106	1	-0.12605923514189143
1106	2	-0.2205145826401278
1106	3	-0.19078896078852736
1106	4	-0.10333780780888446
1106	5	-0.493088800735336
1106	6	-0.40948830671022307
1106	7	-0.8109260976207509
1106	8	-0.27427377686929333
1106	9	0.01990780489069266
1106	10	-0.4197038407368723
1106	11	-0.4333547621594178
1106	12	-0.9022819569130291
1106	13	-0.3049252567544656
1106	14	-0.35974859672250403
1106	15	-0.22575697648854032
1106	16	-0.09434914235982461
1106	17	-0.28150290257347166
1106	18	-0.5380668540652067
1106	19	-0.5336141107426542
1106	20	-0.08642075990846365
1106	21	0.0665958901941579
1106	22	0.10559792787525879
1106	23	-0.6202615913941187
1106	24	0.04665754067298877
1106	25	0.0939002622233076
1107	1	-1.1302448414523738
1107	2	-1.2494551474056266
1107	3	-1.0464327224184444
1107	4	-0.9092136698100575
1107	5	-0.9990116027859557
1107	6	-1.0486907759974073
1107	7	-1.309795474936223
1107	8	-1.1029819825694105
1107	9	-0.981855454282794
1107	10	-0.8926753489417436
1107	11	-0.7959663207682581
1107	12	-0.9718283160648156
1107	13	-0.8589856680419174
1107	14	-1.2632360263868065
1107	15	-1.1007041058762255
1107	16	-1.154687542735033
1107	17	-1.115509940088515
1107	18	-1.2305539109512722
1107	19	-1.3379605083881216
1107	20	-1.2908389839784533
1107	21	-1.209795696658908
1107	22	-1.1469511793842457
1107	23	-1.0513535600021058
1107	24	-1.0443133487355283
1107	25	-0.9722689744401691
1108	1	-0.07755919743913964
1108	2	-0.2842118664730364
1108	3	-0.08331814752748201
1108	4	-0.2471486351287595
1108	5	-0.48087234948044255
1108	6	-0.046506818509158165
1108	7	0.02920587719887635
1108	8	0.12557138880268326
1108	9	-0.21662054580868073
1108	10	-0.6645650060404193
1108	11	-0.04062921745584447
1108	12	0.1059996173717721
1108	13	0.12877647549727556
1108	14	-0.2651923682529258
1108	15	-0.07622178311896732
1108	16	-0.743428992743155
1108	17	-0.7158341948973401
1108	18	-0.6482980655685466
1108	19	-0.17578107270537657
1108	20	-1.1071184329147599
1108	21	-0.27321181564136765
1108	22	-0.34742393516708603
1108	23	-0.17869238254971265
1108	24	-0.3725820226228507
1108	25	-0.9554843984746881
1109	1	0.10563324008412422
1109	2	0.0501813491408889
1109	3	0.17382478631125764
1109	4	0.29981148531445
1109	5	0.09430040525040795
1109	6	0.07359761498384643
1109	7	0.16860650108699105
1109	8	0.2806731932704807
1109	9	0.21766494496333166
1109	10	0.17212289552414006
1109	11	0.12844542593295927
1109	12	0.24616835049936014
1109	13	0.21182074753668445
1109	14	0.5199989611244291
1109	15	0.37349223437887247
1109	16	0.41521796921799137
1109	17	0.3529766913597072
1109	18	0.2951077705715832
1109	19	0.28282736601083514
1109	20	0.3314641088896977
1109	21	0.3184883534292169
1109	22	0.23902185393874475
1109	23	0.2633438295196555
1109	24	0.3610759205448488
1109	25	0.40976395319389525
1110	1	-0.057874817388043454
1110	2	0.07155406545650678
1110	3	0.17051380094480706
1110	4	-1.064499619616555
1110	5	-0.3238485577598951
1110	6	-0.09222857368084192
1110	7	0.04691551479567835
1110	8	0.13651978973697965
1110	9	0.08061386990246433
1110	10	-0.06242804212630659
1110	11	0.03361829684808271
1110	12	0.13240108665053185
1110	13	0.0243938570102884
1110	14	0.264479920939813
1110	15	0.056273791548683505
1110	16	0.008530192220263189
1110	17	0.11421870708785775
1110	18	0.2738709842450717
1110	19	0.17141015671433735
1110	20	0.15715723474595933
1110	21	0.15189670396644317
1110	22	-0.35196330148360744
1110	23	0.06153726882239141
1110	24	0.17275625261669347
1110	25	0.249606664162215
1111	1	-0.015795385541171214
1111	2	-0.27211545379440594
1111	3	-0.10082025224971147
1111	4	0.06101145496428728
1111	5	0.004633931885386744
1111	6	0.0035807598029080623
1111	7	-0.0030899647446887
1111	8	0.2470479744512062
1111	9	0.028199541441622955
1111	10	-0.0059636349642246955
1111	11	-0.07452005617398783
1111	12	0.10431102879302756
1111	13	-0.07859136434702663
1111	14	-0.13351558798592578
1111	15	0.017701856465123456
1111	16	-0.04755413898034533
1111	17	0.0697805479985405
1111	18	0.026818869518542954
1111	19	-0.011174628428584299
1111	20	0.08710566260135977
1111	21	-0.004165118141891355
1111	22	-0.06587026748359648
1111	23	0.08720805588852443
1111	24	0.08248275908739089
1111	25	0.10004615553194185
1112	1	-0.08418208444897601
1112	2	-0.37244949578361997
1112	3	-0.1254900240192427
1112	4	-0.1151615328046347
1112	5	-0.005400473087107116
1112	6	-0.01742044653496322
1112	7	-0.076446829780906
1112	8	0.023928536182708626
1112	9	-0.04122239010546025
1112	10	0.24094570576942687
1112	11	-0.027405097043420312
1112	12	0.06491452230422823
1112	13	0.06524462779825058
1112	14	-0.17708564484068745
1112	15	-0.15001940916182122
1112	16	-0.008714224884585065
1112	17	-0.14049588189050866
1112	18	-0.021817049056359394
1112	19	0.06348259309940829
1112	20	-0.6074604614837398
1112	21	-1.0388371078420349
1112	22	-1.5113668405103127
1112	23	-0.4766750537583917
1112	24	-0.23644300515199745
1112	25	-1.277767830413957
1113	1	-0.15402282899997388
1113	2	-0.09391544846661404
1113	3	-0.17903384344819884
1113	4	-0.48883989962693875
1113	5	-0.24325519675416274
1113	6	-0.19470742048958556
1113	7	-0.22222499764380643
1113	8	-0.05618679472218702
1113	9	-0.23115499650557325
1113	10	-0.013091155676015376
1113	11	-0.25242809847666337
1113	12	-0.20181433416282601
1113	13	-0.17430968396129742
1113	14	-0.5575708371934169
1113	15	-0.5247513255589098
1113	16	-0.44577438991937157
1113	17	-0.49778092140661556
1113	18	-0.8005401437742989
1113	19	-0.9508861284685395
1113	20	-1.2739287282987704
1113	21	-1.2180099195484289
1113	22	-0.479081061591053
1113	23	-1.8663597003116905
1113	24	-0.9505021366210755
1113	25	-0.3779719056843973
1114	1	-0.28512053372962853
1114	2	-0.38063959187118
1114	3	-0.272868052932278
1114	4	-0.5167934540094379
1114	5	-0.5553236983672463
1114	6	-0.32263755533925575
1114	7	-0.39544830080757265
1114	8	-0.12564002149144216
1114	9	-1.1482252337662615
1114	10	-0.3538173589586009
1114	11	-0.41591382010793154
1114	12	-0.2729517376027957
1114	13	-0.3145738458829044
1114	14	-0.6486489840109897
1114	15	-1.291375941153298
1114	16	-1.257509761688837
1114	17	-1.5938282767428618
1114	18	-1.7410700393283918
1114	19	-1.5343555292876019
1114	20	-0.7677656960833801
1114	21	-0.48790020684364066
1114	22	-0.4242627529288967
1114	23	-0.3117888060030012
1114	24	-0.8168107966959255
1114	25	-0.4774919613916566
1115	1	-0.5399551330709471
1115	2	-0.292865618833936
1115	3	-0.3293394048857827
1115	4	-0.5115402976750048
1115	5	-0.317417549713376
1115	6	-0.26658352832659205
1115	7	-0.17804533698262048
1115	8	0.018865502454254113
1115	9	-0.28274410896440116
1115	10	-0.003952889951641225
1115	11	-0.20515885307607817
1115	12	-0.36555645654126323
1115	13	-0.17934850666745916
1115	14	-0.18471492890124352
1115	15	-1.189980398162696
1115	16	-0.6000817394934472
1115	17	-0.4558861311914555
1115	18	-1.8605958868938473
1115	19	-0.9143354976904448
1115	20	-0.5590950469344307
1115	21	-0.5304030219520186
1115	22	-0.3536010947207515
1115	23	-0.07609563294621324
1115	24	-0.11852315013282322
1115	25	-0.17658972164645093
1116	1	-1.2427823849322692
1116	2	-0.5945439077154191
1116	3	-1.4912683833454174
1116	4	-0.7715897350667973
1116	5	-0.7396363995927544
1116	6	-0.30594012983613084
1116	7	-0.9976919880111836
1116	8	-2.777691319007893
1116	9	-1.2154789792450487
1116	10	-0.5436337805667719
1116	11	-0.39090712606962086
1116	12	-0.5046137770524339
1116	13	-1.3241012937356658
1116	14	-1.3784364840344665
1116	15	-1.3408601715016815
1116	16	-0.6599865454283034
1116	17	-1.5127109327035129
1116	18	-2.056636814108387
1116	19	-1.0140169226034175
1116	20	-0.6959105427591155
1116	21	-0.3394972906429814
1116	22	-0.26333295883397057
1116	23	-0.2035111971907071
1116	24	-0.3363641712947256
1116	25	-0.25181292085865437
1201	1	0.3209978352819469
1201	2	0.2532862267178402
1201	3	0.34065959826562775
1201	4	0.37034401072349254
1201	5	0.3260107141566948
1201	6	0.22507489229202093
1201	7	0.1577145864442855
1201	8	0.1621804397579112
1201	9	0.16499728488977183
1201	10	0.4048849523105389
1201	11	0.3373289833632711
1201	12	0.3565352081746198
1201	13	0.2664865914264567
1201	14	0.6664822697180028
1201	15	0.6504504223169221
1201	16	0.5021730753206697
1201	17	0.5448261312981515
1201	18	0.37670651799155763
1201	19	0.43098171157760135
1201	20	0.5291760727320423
1201	21	0.3913020214167986
1201	22	0.5110517291363408
1201	23	0.6175746265485955
1201	24	0.5944455783490099
1201	25	0.7015938454226784
1202	1	0.3064962674847038
1202	2	0.4278931771066006
1202	3	0.3837581728787107
1202	4	0.4565845170293912
1202	5	0.3268255259830397
1202	6	0.08505372722571991
1202	7	0.03941400844595222
1202	8	0.17593131443132393
1202	9	0.27420944614457454
1202	10	0.38898377606309253
1202	11	0.15328983781473937
1202	12	0.19730476097319638
1202	13	0.16812365520642913
1202	14	0.5726743156455996
1202	15	0.28203450924694806
1202	16	0.39685506096489576
1202	17	0.36374004823960604
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2210	18	0.5208007971647319
2210	19	0.5076653711421457
2210	20	0.5520230813884552
2210	21	0.4939581044034824
2210	22	0.5572151254613189
2210	23	0.6694735391952094
2210	24	0.5891978619517033
2210	25	0.5387999787362432
2211	1	0.10880350475737899
2211	2	0.0407514745977231
2211	3	0.10976155147796973
2211	4	0.19056833669232986
2211	5	0.20482140500044466
2211	6	0.07868228278374545
2211	7	0.2084591523402537
2211	8	0.27993875216787956
2211	9	0.11180955597991016
2211	10	0.3961516585512612
2211	11	0.12657314676851428
2211	12	0.26408582147270465
2211	13	0.042967281577624306
2211	14	-0.020175049034754977
2211	15	0.052359335515284045
2211	16	0.10819648856180919
2211	17	0.21977521184830096
2211	18	0.2535834200567848
2211	19	0.19219471484377948
2211	20	0.16922852473810934
2211	21	0.10386774818754674
2211	22	0.2449461090525915
2211	23	0.28063555230840365
2211	24	0.20451648573492065
2211	25	0.24557473388350104
2212	1	0.08583083470382746
2212	2	0.07322389554882136
2212	3	0.09301453237572943
2212	4	0.05017272760338898
2212	5	0.16316647526601713
2212	6	0.18758548265179797
2212	7	0.0840474406402052
2212	8	0.2643489928154992
2212	9	-0.02578346473602977
2212	10	0.2610616027595327
2212	11	0.12396420451040058
2212	12	0.20153340328964475
2212	13	0.06979843935956609
2212	14	0.09777623769034705
2212	15	0.0407700215118183
2212	16	0.03155906180398205
2212	17	0.1129758504506242
2212	18	0.07092838057717188
2212	19	0.16798305990555917
2212	20	0.12864101017352425
2212	21	0.14070047042206824
2212	22	-0.401714792984869
2212	23	0.06589135091899578
2212	24	0.11578474732243649
2212	25	0.25074889014578616
2213	1	-0.04059036444675712
2213	2	-0.756667691049433
2213	3	-0.09752682165345661
2213	4	0.07077702638395067
2213	5	0.031280855010265896
2213	6	0.11283383044322193
2213	7	0.1254990994114351
2213	8	0.18259798795994442
2213	9	-0.12678268937077064
2213	10	-1.2035804855575662
2213	11	-0.28147019853531674
2213	12	0.04719358589343159
2213	13	-0.0043652072715687495
2213	14	0.2228630307974555
2213	15	0.18435397525889385
2213	16	0.03707250025318842
2213	17	0.019332265686671235
2213	18	0.23551039226517645
2213	19	0.12634870012472987
2213	20	0.190188564694175
2213	21	0.1405309352280213
2213	22	0.15271754971199414
2213	23	-0.7339505782740332
2213	24	-1.3406880537230563
2213	25	-1.1707024364417933
2214	1	0.06188174693764026
2214	2	-0.2244441665830159
2214	3	0.011530380291727482
2214	4	-0.02643082301992905
2214	5	-0.03205514163973078
2214	6	-0.08655994413429369
2214	7	-0.031963080689742185
2214	8	-0.03824992542771355
2214	9	-0.35389160297822997
2214	10	-0.11868084927677813
2214	11	-0.1863613154116024
2214	12	-0.14096933528362854
2214	13	-0.06013200983156253
2214	14	-0.06125409577266083
2214	15	-0.11890934974756107
2214	16	-0.25216709877325627
2214	17	-0.1910753661595379
2214	18	-0.07812121649039483
2214	19	-0.7805681819336598
2214	20	-0.30436567471232223
2214	21	-0.2955560830285293
2214	22	-0.41604896565903715
2214	23	-1.7568619203128861
2214	24	-1.2876585711284676
2214	25	-1.5467934336292686
2215	1	-0.44125643321896035
2215	2	-0.22735487931827336
2215	3	-1.1495498638926485
2215	4	-0.9258940239126758
2215	5	-0.34486715912171206
2215	6	-0.13188997995691665
2215	7	-0.16254228436571339
2215	8	-0.11675479427083957
2215	9	-0.4056776023484474
2215	10	-1.3437458585455246
2215	11	-0.5845107268971645
2215	12	-0.5833725636004985
2215	13	-0.6479303332546209
2215	14	-0.8884665315386187
2215	15	-0.9929275587447108
2215	16	-0.7658317989903978
2215	17	-0.6095250946590625
2215	18	-0.4677103907216349
2215	19	-1.0528307305530689
2215	20	-1.455631910024465
2215	21	-0.7772288008220869
2215	22	-0.46068049444416914
2215	23	-0.6484811962662747
2215	24	-1.7234972036991607
2215	25	-0.9176550069996097
2216	1	-0.295595357702247
2216	2	-0.2583921520977803
2216	3	-1.3312173693742615
2216	4	-0.5376187152140375
2216	5	-0.43382796745112273
2216	6	-0.2537982568643571
2216	7	-0.16863965464997932
2216	8	-0.27651927459891823
2216	9	-0.11414795021958213
2216	10	-0.08549078565014412
2216	11	-0.20851721041710647
2216	12	-0.13729001877544533
2216	13	-0.8677836230634455
2216	14	-0.43767889937462456
2216	15	-0.9742921323889959
2216	16	-0.6543506003435269
2216	17	-1.6523572237531994
2216	18	-0.6958353658647383
2216	19	-0.4050439298261992
2216	20	-0.3845656009250078
2216	21	-0.1270690274488544
2216	22	-0.16739078838082833
2216	23	-0.1889688347096481
2216	24	-0.3018992940849614
2216	25	-0.20389738300177385
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3461.0
3	398.0
4	456.0
5	388.0
6	275.0
7	217.0
8	423.0
9	889.0
10	1735.0
11	2940.0
12	5032.0
13	7213.0
14	9725.0
15	12471.0
16	14685.0
17	15649.0
18	16331.0
19	16577.0
20	17343.0
21	18418.0
22	19525.0
23	20535.0
24	21393.0
25	22399.0
26	23350.0
27	25997.0
28	29193.0
29	33697.0
30	40883.0
31	50818.0
32	67797.0
33	94783.0
34	134965.0
35	214537.0
36	390306.0
37	1335672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67296714391637	20.600577387243842	12.519146704888712	23.207308763951083
2	14.410683973102497	21.470846971217423	40.070226115336645	24.04824294034344
3	18.707108289164328	27.12455846630311	29.510172441093196	24.658160803439365
4	11.64491776799707	18.137500675509337	38.10079049603401	32.11679106045959
5	11.898044432699296	39.70113934618226	35.4796425203755	12.921173700742946
6	30.620191541051884	38.1098781528764	17.72758397508653	13.54234633098519
7	25.29891845713842	33.331007192652095	23.572884553262806	17.79718979694668
8	24.514380025353336	37.54162698171453	21.265580330974377	16.67841266195776
9	24.4522009720386	17.02868868608167	21.750405016082432	36.7687053257973
10	14.376613345723428	31.069801620870603	34.21231198118667	20.341273052219297
11	31.516962091050072	26.463511045723898	22.387603845038306	19.631923018187727
12	20.654769208601024	28.942050831182254	31.7863185611378	18.61686139907893
13	26.871391139155946	23.91464905716458	26.002262234568356	23.211697569111116
14	19.90634647302048	24.42819302127749	28.22923237354541	27.436228132156614
15	24.271265451183567	29.962463904714447	23.667963895709388	22.098306748392595
16	20.615382768126803	31.513545782798445	27.31688775504461	20.554183694030144
17	20.79575971041998	30.855265897990357	28.52135810237586	19.827616289213804
18	20.880317086157422	29.826526434915802	29.53050580915619	19.762650669770586
19	20.928153070923106	29.94525730268483	29.72120586278566	19.405383763606405
20	22.03496862444337	29.098258547794252	28.725519036205338	20.14125379155704
21	21.277880230152014	29.822245651819095	28.76464949093781	20.135224627091077
22	20.654077751795807	29.549779820314164	28.11290850310833	21.683233924781696
23	19.74533621713138	30.26798458839086	28.72348409233157	21.263195102146188
24	20.470123576884276	30.561860498630928	28.24190415285584	20.726111771628958
25	21.21731474886424	28.787226042073993	26.984306876408677	23.01115233265309
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7121.0
1	7121.0
2	6417.0
3	5713.0
4	5713.0
5	5713.0
6	7584.5
7	9456.0
8	9456.0
9	9456.0
10	12194.5
11	14933.0
12	14933.0
13	14933.0
14	22321.0
15	29709.0
16	29709.0
17	29709.0
18	44196.0
19	58683.0
20	58683.0
21	58683.0
22	80925.0
23	103167.0
24	103167.0
25	103167.0
26	130897.0
27	158627.0
28	158627.0
29	158627.0
30	187401.0
31	216175.0
32	216175.0
33	216175.0
34	238600.5
35	261026.0
36	261026.0
37	261026.0
38	275384.5
39	289743.0
40	289743.0
41	289743.0
42	292182.5
43	294622.0
44	294622.0
45	294622.0
46	340804.0
47	386986.0
48	386986.0
49	386986.0
50	347138.5
51	307291.0
52	307291.0
53	307291.0
54	258421.5
55	209552.0
56	209552.0
57	209552.0
58	176493.5
59	143435.0
60	143435.0
61	143435.0
62	117666.5
63	91898.0
64	91898.0
65	91898.0
66	70291.0
67	48684.0
68	48684.0
69	48684.0
70	34988.0
71	21292.0
72	21292.0
73	21292.0
74	14879.5
75	8467.0
76	8467.0
77	8467.0
78	5603.5
79	2740.0
80	2740.0
81	2740.0
82	1783.5
83	827.0
84	827.0
85	827.0
86	530.5
87	234.0
88	234.0
89	234.0
90	149.0
91	64.0
92	64.0
93	64.0
94	41.0
95	18.0
96	18.0
97	18.0
98	15.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30473967936802276
2	0.17978068329391464
3	0.19973967187872121
4	0.21793867460332914
5	0.24310272775340427
6	0.19663161174262567
7	0.19161377971567617
8	0.21486806097489736
9	0.21595400969714762
10	0.2240050088448651
11	0.1994775463250746
12	0.2073413129344731
13	0.21921185586389844
14	0.20771577801111113
15	0.1987660626794624
16	0.2023234809075236
17	0.20127497869293715
18	0.20442048533669652
19	0.20457027136735173
20	0.20011413695535926
21	0.19775500697253973
22	0.20146221123125613
23	0.20490728993632595
24	0.22093439521643335
25	0.21501784700555257
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2670476.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.62592208531163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32490266012259	69.21228953597911
2	7.638387791619546	11.705970155560696
3	1.153826006648659	2.6523894505639913
4	0.3151628408623576	0.9659857315241793
5	0.14762416561797784	0.5655918906276155
6	0.08481954437589581	0.38996254791954144
7	0.05656071367699437	0.30338117875120935
8	0.04204997493505924	0.2577694482450525
9	0.026240296491024998	0.1809618222975082
>10	0.1793484641022649	2.5391294425933415
>50	0.01673985456046863	0.884131057103003
>100	0.010995353854993884	1.621311482861087
>500	9.830391565480655E-4	0.5205799336311779
>1k	0.0018186224395761723	3.1341382706134095
>5k	3.440637047846812E-4	1.9190500186572739
>10k+	1.966078313055321E-4	3.1473580330719244
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	30765	1.1520418082768764	No Hit
CCCATGTACTCTGCGTTGATACCAC	27331	1.0234505009593795	No Hit
GAGTACATGGGAAGCAGTGGTATCA	14716	0.551062806780514	No Hit
CATGTACTCTGCGTTGATACCACTG	10754	0.4026997434165295	No Hit
GCTTCCCATGTACTCTGCGTTGATA	8911	0.3336858297921419	No Hit
GCGTTGATACCACTGCTTCCCATGT	8339	0.312266427408447	No Hit
GTATCAACGCAGAGTACATGGGAAG	7663	0.2869525882277167	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	7430	0.2782275519420508	No Hit
TATCAACGCAGAGTACATGGGAAGC	6674	0.24991799214821625	No Hit
GGTATCAACGCAGAGTACATGGGAA	6143	0.23003389657873727	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5793	0.2169276188964065	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3876	0.14514266370489753	No Hit
GTGGTATCAACGCAGAGTACATGGG	3530	0.13218617205322197	No Hit
CAGTGGTATCAACGCAGAGTACATG	3315	0.12413517290550448	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3124	0.11698288994171827	No Hit
ACATGGGAAGCAGTGGTATCAACGC	3016	0.11293866711402761	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3015	0.11290122060636382	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2912	0.10904423031699217	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2828	0.10589872367323279	No Hit
CATGGGAAGCAGTGGTATCAACGCA	2790	0.10447575638200829	No Hit
ATTCCATTCCATTCCATTCCATTCC	2769	0.10368937972106845	No Hit
ATACCACTGCTTCCCATGTACTCTG	2695	0.10091833815394709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.744650766380226E-5	0.0
4	0.0	0.0	0.0	3.744650766380226E-5	0.0
5	0.0	0.0	0.0	3.744650766380226E-5	0.0
6	0.0	0.0	0.0	3.744650766380226E-5	0.0
7	0.0	0.0	0.0	3.744650766380226E-5	0.0
8	0.0	0.0	0.0	3.744650766380226E-5	0.0
9	0.0	0.0	0.0	3.744650766380226E-5	0.0
10	0.0	0.0	0.0	7.489301532760452E-5	0.0
11	0.0	0.0	0.0	7.489301532760452E-5	0.0
12	0.0	0.0	0.0	7.489301532760452E-5	0.0
13	0.0	0.0	0.0	7.489301532760452E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAGAA	90	7.4326053E-6	11.615795	2
TAGACAG	400	0.0	11.405669	5
TAGGACT	315	0.0	10.8633585	4
CTTACAC	450	0.0	10.348617	3
GACTGTC	170	1.5606929E-9	10.058723	7
GTCTTAC	275	0.0	10.02825	1
GTAGGAC	175	2.557499E-9	9.775369	3
GTCTTGG	330	0.0	9.509547	1
GTCCTAT	160	6.5379936E-8	9.509546	1
CTAGACT	330	0.0	9.505438	4
GGACCTT	130	4.246571E-6	9.499014	6
CCTATAC	245	1.8189894E-12	9.309876	3
TATACTG	655	0.0	9.287058	5
TACACTG	595	0.0	9.265109	5
GTCTTAG	300	0.0	9.192561	1
TTAGGAC	230	4.1836756E-11	9.090621	3
GTATTAT	600	0.0	9.034069	1
TATTCCG	95	0.0018161145	9.004475	5
CGGGTTC	190	1.0633812E-8	8.997546	18
CTAGGAC	225	2.6011548E-10	8.870243	3
>>END_MODULE