FastQCFastQC Report
Wed 25 May 2016
SRR1295039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670476
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC306021.1459380275276767No Hit
GTACATGGGAAGCAGTGGTATCAAC292711.096096725827156No Hit
GAGTACATGGGAAGCAGTGGTATCA159210.5961858485153957No Hit
CATGTACTCTGCGTTGATACCACTG121750.4559112308067925No Hit
GCTTCCCATGTACTCTGCGTTGATA101440.3798573737416101No Hit
GTATCAACGCAGAGTACTTTTTTTT97390.3646915381377702No Hit
ACGCAGAGTACATGGGAAGCAGTGG92780.34742869810475735No Hit
GCGTTGATACCACTGCTTCCCATGT90590.33922791292638466No Hit
GTATCAACGCAGAGTACATGGGAAG80880.30286735398483267No Hit
GGTATCAACGCAGAGTACTTTTTTT77630.29069723899409694No Hit
TATCAACGCAGAGTACATGGGAAGC74400.2786020170186888No Hit
GGTATCAACGCAGAGTACATGGGAA68120.255085610205821No Hit
TATCAACGCAGAGTACTTTTTTTTT67680.25343796386861367No Hit
ACTCTGCGTTGATACCACTGCTTCC66350.248457578349328No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41360.15487875569748616No Hit
CAGTGGTATCAACGCAGAGTACATG39450.1477264727336999No Hit
GTGGTATCAACGCAGAGTACATGGG38450.14398182196731968No Hit
ACATGGGAAGCAGTGGTATCAACGC38380.14371969641367308No Hit
CATGGGAAGCAGTGGTATCAACGCA34860.13053852571601468No Hit
GCAGTGGTATCAACGCAGAGTACAT34770.13020150714704046No Hit
GGGAAGCAGTGGTATCAACGCAGAG32470.12158881038436595No Hit
ATACCACTGCTTCCCATGTACTCTG27910.1045132028896721No Hit
ATTCCATTCCATTCCATTCCATTCC26910.10076855212329187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT551.9622446E-413.8171348
GACTGCG604.0963574E-412.6657067
CGCAAAA701.09185814E-412.2138172
TAGACTG3050.011.5237165
TGGCGTA600.005880851311.0824935
GAACGAA600.005880851311.08249316
GGACTGT3650.010.9306776
TTTAGAC1803.6379788E-1110.5547553
CTAGGAC3150.010.253193
TTAGACT2450.010.0808684
TTAGACA2750.010.0174224
GTCTAGC1651.0009899E-89.80089951
TAAGGGT1606.6478606E-89.4992794
ATTAGAC2750.09.3265653
TCTACAC4100.09.267593
TGGACTG3900.09.2557085
GTATAGG2501.8189894E-129.1321321
GGCCTAC1252.7124765E-59.1321321
ACACCGT1151.1120016E-49.08626656
GACAGGT2201.6916601E-109.0674937
Produced by FastQC (version 0.11.5)