FastQCFastQC Report
Wed 25 May 2016
SRR1295038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533131
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC219370.866003376848651No Hit
CCCATGTACTCTGCGTTGATACCAC186080.7345849859324292No Hit
GAGTACATGGGAAGCAGTGGTATCA108900.42990275670701594No Hit
CATGTACTCTGCGTTGATACCACTG74370.2935892379825599No Hit
GCTTCCCATGTACTCTGCGTTGATA63190.24945413403412614No Hit
GCGTTGATACCACTGCTTCCCATGT60610.23926911004602605No Hit
ACGCAGAGTACATGGGAAGCAGTGG56750.22403105090103909No Hit
GTATCAACGCAGAGTACATGGGAAG55990.2210308112766375No Hit
GGTATCAACGCAGAGTACATGGGAA52500.2072533951066881No Hit
TATCAACGCAGAGTACATGGGAAGC51110.20176611474100628No Hit
GTATCAACGCAGAGTACTTTTTTTT46040.1817513582992747No Hit
ACTCTGCGTTGATACCACTGCTTCC42580.16809237264081486No Hit
GGTATCAACGCAGAGTACTTTTTTT40360.1593285147905892No Hit
TATCAACGCAGAGTACTTTTTTTTT30710.12123336692812176No Hit
GTGGTATCAACGCAGAGTACATGGG30020.11850946516386243No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN29590.11681196116584575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGC700.001492753210.8564537
GTCCTAG3050.010.2886231
TTAGACC1451.6066224E-79.832414
CTAGGTC1106.802622E-59.50466254
CTTAGAC2600.09.50334553
TCTGTCG2201.6370905E-119.4992088
TAGACTG3350.09.0785425
TCGCTCT2002.6338967E-99.0294294
TATACTG5900.09.020845
TAACCCG950.00181713499.0038825
GTTAGAC950.00181844489.003173
GTCCAAG2451.6370905E-118.9269861
GTCTAGC1606.650371E-78.9148581
GTCTATG3100.08.8956861
GTCTAAG2252.564775E-108.87523651
AGAACTG3650.08.8531325
GCCTAGA2054.0708983E-98.8133871
GTCATAG2401.0004442E-108.7167491
CAGGACC2304.0017767E-108.678174
AGGCTAT1651.0519361E-68.6354726
Produced by FastQC (version 0.11.5)