FastQCFastQC Report
Wed 25 May 2016
SRR1295038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533131
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC212540.8390406970661999No Hit
CCCATGTACTCTGCGTTGATACCAC209070.825342234570577No Hit
GAGTACATGGGAAGCAGTGGTATCA119810.4729719860520439No Hit
GTATCAACGCAGAGTACTTTTTTTT111250.43917981344036294No Hit
GGTATCAACGCAGAGTACTTTTTTT106370.41991511690473177No Hit
TATCAACGCAGAGTACTTTTTTTTT84230.3325133994254541No Hit
CATGTACTCTGCGTTGATACCACTG84060.3318422931936801No Hit
ACGCAGAGTACATGGGAAGCAGTGG72940.28794405026822534No Hit
GCTTCCCATGTACTCTGCGTTGATA69430.27408768042394965No Hit
GCGTTGATACCACTGCTTCCCATGT65040.2567573489093142No Hit
GTATCAACGCAGAGTACATGGGAAG60960.2406507993467373No Hit
GGTATCAACGCAGAGTACATGGGAA58400.23054472903296358No Hit
TATCAACGCAGAGTACATGGGAAGC57890.22853141033764143No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49880.19691046376993532No Hit
ACTCTGCGTTGATACCACTGCTTCC49670.19608145018950857No Hit
GTGGTATCAACGCAGAGTACATGGG33930.13394490849466528No Hit
ACATGGGAAGCAGTGGTATCAACGC29900.1180357431179043No Hit
CAGTGGTATCAACGCAGAGTACATG28570.11278532377520152No Hit
CATGGGAAGCAGTGGTATCAACGCA28390.1120747407062643No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26770.10567949308582934No Hit
GGGAAGCAGTGGTATCAACGCAGAG25590.10102122630057428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGT400.0052823214.2491866
CGTAAAC550.003070677212.0902193
TACACCG1053.4707955E-610.8565235
GTCTAGG1701.364242E-1010.628791
CGTCTCA951.6456991E-49.99942912
GTGTAGG2950.09.9935271
TAGGGCT2201.8189894E-129.9312514
GTAGGAC1356.451719E-79.851293
GTACTAG1451.5936166E-79.83791
CTAGGAC2251.8189894E-129.7105573
GACTGTG3350.09.6414317
CGCAAAA800.0045185019.4996452
GACTCCG900.00111440459.4996457
ACACCGT1002.757959E-49.4994576
AAGGCGG800.00451923679.4994575
TTACACC2401.8189894E-129.4994574
AATACTG5700.09.33285
ACTGGAC2252.7284841E-119.2883593
GTCTCGC1651.0491749E-79.2217881
TAGGACT2900.09.171894
Produced by FastQC (version 0.11.5)