FastQCFastQC Report
Wed 25 May 2016
SRR1295037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890348
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC246350.8523195130828536No Hit
CCCATGTACTCTGCGTTGATACCAC219700.7601160829076637No Hit
GAGTACATGGGAAGCAGTGGTATCA124380.43032880469756585No Hit
CATGTACTCTGCGTTGATACCACTG91280.3158097225662792No Hit
GCGTTGATACCACTGCTTCCCATGT73230.2533604949992181No Hit
GCTTCCCATGTACTCTGCGTTGATA71810.24844759177787587No Hit
ACGCAGAGTACATGGGAAGCAGTGG65130.22533618789156185No Hit
GTATCAACGCAGAGTACATGGGAAG61180.21167001343782824No Hit
GGTATCAACGCAGAGTACATGGGAA58580.2026745568353707No Hit
TATCAACGCAGAGTACATGGGAAGC57480.19886878673433095No Hit
ACTCTGCGTTGATACCACTGCTTCC51950.1797361424991039No Hit
GTATCAACGCAGAGTACTTTTTTTT45980.15908119022346098No Hit
GGTATCAACGCAGAGTACTTTTTTT42870.14832124021052134No Hit
GTGGTATCAACGCAGAGTACATGGG38000.13147205803591816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35430.12258039516348897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN33590.11621437972174978No Hit
TATCAACGCAGAGTACTTTTTTTTT30490.10548902761881959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT602.5516008E-514.2607231
TATTCCG1355.6639692E-810.5592845
TATACTG6800.010.0623765
CTAGTAC2450.09.6966293
TTAGACA3350.09.6453654
TAGGCCG800.0045042839.5033575
CTAGCAC3100.09.5026973
TCTATAC3650.09.3725233
TTCCGTA950.00182491238.9997457
GTCTTGC5300.08.9690081
ATACTGT8000.08.9055366
TACACAG6850.08.8790485
GTTCTAG3550.08.8376311
GTGTAAG4150.08.705341
CACACCG1107.121933E-48.6394155
ACACCGT1107.152714E-48.6356716
TAGACAG3450.08.5392485
CTTATAC3450.08.5386553
CCAAGAC3700.08.4753783
AGTACTG3600.08.4474285
Produced by FastQC (version 0.11.5)