##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295037_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2890348
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197486254250354	33.0	33.0	33.0	27.0	33.0
2	31.10912353806531	33.0	33.0	33.0	27.0	33.0
3	31.221171983442826	33.0	33.0	33.0	27.0	33.0
4	34.73230732077937	37.0	37.0	37.0	27.0	37.0
5	34.98188626421455	37.0	37.0	37.0	33.0	37.0
6	34.9417966279493	37.0	37.0	37.0	33.0	37.0
7	34.92202184650429	37.0	37.0	37.0	33.0	37.0
8	34.85106464688681	37.0	37.0	37.0	33.0	37.0
9	34.75633072557353	37.0	37.0	37.0	27.0	37.0
10	34.7060796831385	37.0	37.0	37.0	27.0	37.0
11	34.84999453353022	37.0	37.0	37.0	33.0	37.0
12	34.77945250883285	37.0	37.0	37.0	33.0	37.0
13	34.67255430833934	37.0	37.0	37.0	27.0	37.0
14	36.088779621000654	40.0	37.0	40.0	27.0	40.0
15	36.23531837688749	40.0	37.0	40.0	27.0	40.0
16	36.308692932477335	40.0	37.0	40.0	27.0	40.0
17	36.24240852658573	40.0	37.0	40.0	27.0	40.0
18	36.1705856872598	40.0	37.0	40.0	27.0	40.0
19	36.2569666351595	40.0	37.0	40.0	27.0	40.0
20	36.26998133096776	40.0	37.0	40.0	27.0	40.0
21	36.264350175134624	40.0	37.0	40.0	27.0	40.0
22	36.176237947818045	40.0	37.0	40.0	27.0	40.0
23	36.08466177775133	40.0	37.0	40.0	27.0	40.0
24	36.143250570519534	40.0	37.0	40.0	27.0	40.0
25	35.00100472330667	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.34868099119541895
1101	2	0.3636215245121086
1101	3	0.352697748139029
1101	4	0.4642444981287568
1101	5	0.2584780517951799
1101	6	0.32728951137963946
1101	7	0.2981747092510787
1101	8	0.3844479234567544
1101	9	0.4088536841527741
1101	10	0.6404751814676786
1101	11	0.42255707069825377
1101	12	0.4682105604364466
1101	13	0.48874388970620686
1101	14	0.8750237431093524
1101	15	0.7665212452532515
1101	16	0.6609398246759994
1101	17	0.6226245002982225
1101	18	0.6477642308865015
1101	19	0.5753059672413627
1101	20	0.6336469369945519
1101	21	0.5660722882029461
1101	22	0.6704306479919495
1101	23	0.7143978246141387
1101	24	0.7077406486162374
1101	25	0.7419337732162461
1102	1	0.44926124008947355
1102	2	0.4225067624389247
1102	3	0.4892731060266833
1102	4	0.5362655453599032
1102	5	0.3558808687163548
1102	6	0.30034384855993324
1102	7	0.42298621234363054
1102	8	0.5077666532961516
1102	9	0.3779927605065012
1102	10	0.5450741392478804
1102	11	0.48728099535030367
1102	12	0.5579959495647557
1102	13	0.5222898278842152
1102	14	0.8214856780823965
1102	15	0.7732763046776796
1102	16	0.7309789767527306
1102	17	0.7084822969023961
1102	18	0.839411514994886
1102	19	0.6895468135870289
1102	20	0.7721830879506868
1102	21	0.68789930786658
1102	22	0.754793173111139
1102	23	0.7821839436513756
1102	24	0.7743491436888235
1102	25	0.849667204012512
1103	1	0.38078037495056805
1103	2	0.37087126765217704
1103	3	0.3664003507951463
1103	4	0.43750113793057466
1103	5	0.22558251435390275
1103	6	0.22043364272591504
1103	7	0.25176124344027784
1103	8	0.3759236566194062
1103	9	0.40558898479385874
1103	10	0.6742424733052985
1103	11	0.43838697306280494
1103	12	0.502837354141505
1103	13	0.44951525470153086
1103	14	0.8625980103794504
1103	15	0.7597415299769921
1103	16	0.706120164695669
1103	17	0.629917674742849
1103	18	0.6416927639188543
1103	19	0.648583076587208
1103	20	0.6671914934983647
1103	21	0.6805594489604019
1103	22	0.7095716904496214
1103	23	0.776524350588403
1103	24	0.7313472571402215
1103	25	0.775389329435697
1104	1	0.3302285660465074
1104	2	0.23487215322645127
1104	3	0.3266390854726424
1104	4	0.2075727849676383
1104	5	0.2684348105110814
1104	6	0.13405691077380766
1104	7	0.15675005827930732
1104	8	0.27501901211942226
1104	9	0.15249145156349897
1104	10	0.3276712853893926
1104	11	0.20419372477595488
1104	12	0.22770304585803558
1104	13	0.2967109326995683
1104	14	0.5976654927621183
1104	15	0.5098351644749428
1104	16	0.4185308555913352
1104	17	0.5016643701213184
1104	18	0.5291721357187598
1104	19	0.5029101660254227
1104	20	0.5194458792862306
1104	21	0.49689179191818056
1104	22	0.5570861939131646
1104	23	0.5267864231443227
1104	24	0.5021987755068409
1104	25	0.6088860723076692
1105	1	0.1564801690213642
1105	2	0.10918098925971975
1105	3	0.14599130114937608
1105	4	0.1566489146204404
1105	5	0.1501642548416342
1105	6	0.07996051100997192
1105	7	0.1117668143065842
1105	8	0.16173126038015795
1105	9	0.07798083204408357
1105	10	0.44924049167153157
1105	11	0.2612369496746041
1105	12	0.32249660230058197
1105	13	0.31506706725748757
1105	14	0.5816511248519376
1105	15	0.38959421928859683
1105	16	0.3685919714516146
1105	17	0.29572816502729893
1105	18	0.3465990610247758
1105	19	0.25315276293540734
1105	20	0.29008241437044546
1105	21	0.316210691578263
1105	22	0.32615098464999903
1105	23	0.5061478546881943
1105	24	0.5129440511496881
1105	25	0.4138710034576931
1106	1	0.10438450850742598
1106	2	-0.09167146584830022
1106	3	-0.056766336072193724
1106	4	-0.044045661579339423
1106	5	-0.4587364188508758
1106	6	-0.3305004794777062
1106	7	-0.6145689231539819
1106	8	-0.1505367838823517
1106	9	0.1506887897325413
1106	10	-0.16149996040812908
1106	11	-0.39030894417708595
1106	12	-0.7220440747223975
1106	13	-0.23742619104014295
1106	14	-0.3287677797596231
1106	15	-0.15383885984604007
1106	16	-0.1271476866836707
1106	17	-0.1533599819065543
1106	18	-0.29725666267712825
1106	19	-0.39065928245182135
1106	20	0.019066263403630046
1106	21	0.013273484160407634
1106	22	0.3077522908135961
1106	23	-0.5080094928005536
1106	24	0.10780777708282585
1106	25	0.270135622612365
1107	1	-1.179150938584005
1107	2	-1.2103607958214475
1107	3	-0.8907790660080046
1107	4	-1.0574846110185945
1107	5	-1.225612289411913
1107	6	-1.188041243273858
1107	7	-1.137718666832093
1107	8	-1.0150213937992092
1107	9	-1.073973396685993
1107	10	-1.0649601264070654
1107	11	-0.9596567593381309
1107	12	-0.9961532305115384
1107	13	-0.9043508848180508
1107	14	-1.1653648194931066
1107	15	-1.120617083973329
1107	16	-1.1087057037430768
1107	17	-1.0558836216773742
1107	18	-1.1869857476726011
1107	19	-1.1994746553032414
1107	20	-1.3195608887668513
1107	21	-1.190611910517859
1107	22	-1.241768272376568
1107	23	-0.9800353152563588
1107	24	-0.8894288647414399
1107	25	-0.7668537978273307
1108	1	0.03434457332725671
1108	2	-0.13398889506211376
1108	3	-0.03825750338280898
1108	4	-0.225430043964856
1108	5	-0.41887210319064394
1108	6	-0.0023801666739444727
1108	7	-0.06964248920184701
1108	8	0.13976280399842977
1108	9	-0.32055357825134223
1108	10	-0.5821484505703509
1108	11	-0.25247699680543434
1108	12	0.03707384295761784
1108	13	-0.01272197659223906
1108	14	-0.4082995890715537
1108	15	-0.03496051247714149
1108	16	-0.7517815623423729
1108	17	-0.7115851784710685
1108	18	-0.805515945015479
1108	19	-0.12993320718889123
1108	20	-1.0202758757967914
1108	21	-0.28888601108260303
1108	22	-0.25299709838907347
1108	23	-0.12779754516282082
1108	24	-0.3577033636114919
1108	25	-1.1585356845261003
1109	1	0.09616703097857027
1109	2	0.017682541709302058
1109	3	0.11720778775223195
1109	4	0.004771761489578807
1109	5	0.08171453252822403
1109	6	-0.01642303590924854
1109	7	0.05980979688342103
1109	8	0.10587735052579461
1109	9	0.22042158925575706
1109	10	0.1807242141513754
1109	11	0.07688215624754946
1109	12	0.19751866594812384
1109	13	0.21031346478334711
1109	14	0.3794621567637222
1109	15	0.2510887366983212
1109	16	0.3037196648423617
1109	17	0.3147886628888017
1109	18	0.3535687778189711
1109	19	0.26654721831754813
1109	20	0.235618037590136
1109	21	0.24459783674225122
1109	22	0.29199680050720644
1109	23	0.27443398575071143
1109	24	0.29077967113256165
1109	25	0.31217477468145916
1110	1	0.09065638654923802
1110	2	-0.1088689952691837
1110	3	0.14281545935861573
1110	4	-0.9075308773738797
1110	5	-0.2688389663839956
1110	6	-0.03960742114040272
1110	7	0.16343401754261322
1110	8	0.20434629484326905
1110	9	0.15197986773529948
1110	10	-0.04328391449094937
1110	11	0.03459998073216042
1110	12	0.013433532603180254
1110	13	0.13583982787675808
1110	14	0.30683084705882635
1110	15	0.09908438613143034
1110	16	0.20859500943021203
1110	17	0.25891565990144016
1110	18	0.3751423175216715
1110	19	0.366213102364469
1110	20	0.22393184868875693
1110	21	0.26144491645137435
1110	22	-0.27172863148683746
1110	23	0.08035197349663292
1110	24	0.3245591060061841
1110	25	0.35000757790795234
1111	1	-0.07369105985216606
1111	2	-0.18248592897588622
1111	3	-0.08752256135501923
1111	4	-0.07134321584120329
1111	5	-0.13996850526423543
1111	6	-0.07124185820527629
1111	7	-0.01648642986873483
1111	8	0.07873231578197704
1111	9	0.04969115408763258
1111	10	0.011226666234378513
1111	11	-0.1761192168146124
1111	12	0.14342288540857595
1111	13	-0.04691858272225602
1111	14	-0.2227814242032622
1111	15	-0.09619037045920464
1111	16	-0.250135536429255
1111	17	0.007635439322200455
1111	18	-0.12688301570273097
1111	19	-0.10592555841054718
1111	20	-0.05015753389972133
1111	21	-0.18736973656270806
1111	22	-0.11646115810272306
1111	23	0.07067573336023969
1111	24	-0.0303291059032631
1111	25	0.026236209107629804
1112	1	-0.013424145018856137
1112	2	-0.34283450435462015
1112	3	-0.03333533349401563
1112	4	-0.012828148688456054
1112	5	-0.021630162613348602
1112	6	-0.09723264015634925
1112	7	-0.017101098386852698
1112	8	0.24629455344299345
1112	9	0.056278651675320646
1112	10	0.25396598270564397
1112	11	0.10158487235651137
1112	12	0.21919324231020454
1112	13	0.07325453084941103
1112	14	0.012738010742701533
1112	15	0.0853806178651979
1112	16	0.08403541586123708
1112	17	-0.10858364915539198
1112	18	0.04495994577263929
1112	19	0.03657868952089416
1112	20	-0.7164964653056174
1112	21	-1.0697882569407184
1112	22	-1.5470459252542668
1112	23	-0.3395586341012873
1112	24	-0.17773571925782505
1112	25	-1.2172762723477177
1113	1	-0.08511775945294886
1113	2	-0.07288287322621656
1113	3	-0.11162956113593481
1113	4	-0.45176040359647374
1113	5	-0.2154014183035855
1113	6	-0.10539030389409021
1113	7	-0.04511123679052531
1113	8	0.07479779996710079
1113	9	-0.07273557686350784
1113	10	-0.01508032878422938
1113	11	-0.005330330580648024
1113	12	-0.0821194458940937
1113	13	-0.18892030211927135
1113	14	-0.4569539756507126
1113	15	-0.31380032586171325
1113	16	-0.25610731982053636
1113	17	-0.33990791082111116
1113	18	-0.731294737279562
1113	19	-0.9746350425555832
1113	20	-1.1051338331215064
1113	21	-1.0111672595902803
1113	22	-0.4784926024192018
1113	23	-2.01656923125293
1113	24	-1.026646266344187
1113	25	-0.5477999122040913
1114	1	-0.3582834079647448
1114	2	-0.48415347584339585
1114	3	-0.45054353439976325
1114	4	-0.6468028462878763
1114	5	-0.6975636679570201
1114	6	-0.45379391774785915
1114	7	-0.3978964424657363
1114	8	-0.26147647198310153
1114	9	-1.2303841440311132
1114	10	-0.4820570858889326
1114	11	-0.3790075737410916
1114	12	-0.34600842598634074
1114	13	-0.3962711164449928
1114	14	-0.8362128644435671
1114	15	-1.3656022567738972
1114	16	-1.1260032864648295
1114	17	-1.4746817139663264
1114	18	-1.699651997740446
1114	19	-1.683514686480315
1114	20	-0.9365056188168381
1114	21	-0.7335384242942666
1114	22	-0.562431900736243
1114	23	-0.39409315113351084
1114	24	-1.0260799378477472
1114	25	-0.4854481386162277
1115	1	-0.7177632609631956
1115	2	-0.5895934039987658
1115	3	-0.5360917833293684
1115	4	-0.6313198067243562
1115	5	-0.46771320714808695
1115	6	-0.38017725721604734
1115	7	-0.5462369764673696
1115	8	-0.3174004752376902
1115	9	-0.6669145313499598
1115	10	-0.3345541070542879
1115	11	-0.5992438468382986
1115	12	-0.7007315372733984
1115	13	-0.4443555445701719
1115	14	-0.48477110135098656
1115	15	-1.5362168699189525
1115	16	-0.9780052449896246
1115	17	-0.6815820591033415
1115	18	-2.3424036217863176
1115	19	-1.167364158232452
1115	20	-0.95046233395869
1115	21	-0.9012680509974444
1115	22	-0.7185001755729417
1115	23	-0.3916523409474735
1115	24	-0.4101057266935584
1115	25	-0.5880252067804719
1116	1	-1.4594479761653893
1116	2	-0.886176145493561
1116	3	-1.6404965659182906
1116	4	-1.0536477975805951
1116	5	-0.9953669638766343
1116	6	-0.46669052618975115
1116	7	-1.37033179558739
1116	8	-3.1185530934882557
1116	9	-1.510515314745561
1116	10	-0.7928598820742252
1116	11	-0.6246876168902133
1116	12	-0.7448064549716591
1116	13	-1.826165108780458
1116	14	-1.710162927005797
1116	15	-1.4216733924723997
1116	16	-1.0511224437408018
1116	17	-1.7478067422570334
1116	18	-2.50909274977424
1116	19	-1.32710340786398
1116	20	-1.0337238947316294
1116	21	-0.7426638928747167
1116	22	-0.5570025846014772
1116	23	-0.5450041437069402
1116	24	-0.6622146374245261
1116	25	-0.5589435320256584
1201	1	0.3240583650604911
1201	2	0.30101360222561624
1201	3	0.33253765506447763
1201	4	0.3816168089815122
1201	5	0.27125299948379933
1201	6	0.09329539041015522
1201	7	0.060593446287519726
1201	8	0.22072746943341315
1201	9	0.1830008755076591
1201	10	0.431927837369841
1201	11	0.286492680951973
1201	12	0.328921059696917
1201	13	0.4098390975341104
1201	14	0.7906486736771114
1201	15	0.6958360079004962
1201	16	0.5770258119022742
1201	17	0.6117978545189047
1201	18	0.6376957718926377
1201	19	0.5910075137957023
1201	20	0.62634492425952
1201	21	0.5487850022788194
1201	22	0.6210115825709153
1201	23	0.7617805121651742
1201	24	0.6440454107882871
1201	25	0.6957140391620626
1202	1	0.3564669358315484
1202	2	0.3860351321289315
1202	3	0.3678578791844451
1202	4	0.49876815213401215
1202	5	0.35026011322464257
1202	6	0.08184382632409637
1202	7	0.1678759636088003
1202	8	0.18705339888061445
1202	9	0.28687591269331847
1202	10	0.4951350935063914
1202	11	0.2881804457581296
1202	12	0.3759136411308148
1202	13	0.252326147857481
1202	14	0.8017992983746112
1202	15	0.48917678484223615
1202	16	0.39971060790034585
1202	17	0.4511460897490167
1202	18	0.5015814153567462
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2210	22	0.45891048398140555
2210	23	0.5662769661287257
2210	24	0.5514154661632631
2210	25	0.5756020782361517
2211	1	0.15835133509835586
2211	2	-0.07983202354719765
2211	3	0.039928895957210386
2211	4	0.04902799929420354
2211	5	0.1495415761617167
2211	6	0.06888231552513702
2211	7	0.13335915269083642
2211	8	0.2689805458870538
2211	9	0.1394477582819249
2211	10	0.24904599215057743
2211	11	0.09743266066902123
2211	12	0.08515169067792527
2211	13	0.012187919883608345
2211	14	-0.04938604627803045
2211	15	0.11128303237648396
2211	16	0.09168274312801827
2211	17	0.13052951895223686
2211	18	0.2090648268634112
2211	19	0.11335600867472806
2211	20	0.028179031764032914
2211	21	0.17349793499718658
2211	22	0.2129223343359712
2211	23	0.26275064342220844
2211	24	0.2502391305920213
2211	25	0.14653909291673983
2212	1	0.16398637567567675
2212	2	0.1411752749222117
2212	3	0.24985674219794518
2212	4	0.22212429180234494
2212	5	0.23669393861219135
2212	6	0.1804379319384637
2212	7	0.14600444935200585
2212	8	0.3605162730586784
2212	9	0.22076339961229507
2212	10	0.42483777858894456
2212	11	0.20754216614172805
2212	12	0.27365866035027153
2212	13	0.2450425974506203
2212	14	0.27629418761637226
2212	15	0.22558848365756745
2212	16	0.1712176067143858
2212	17	0.2749380611825245
2212	18	0.4083892199737278
2212	19	0.20839775810623706
2212	20	0.27153402932350446
2212	21	0.2783227040459053
2212	22	-0.3365103095904729
2212	23	0.2020753868825409
2212	24	0.23742787624910733
2212	25	0.3435609894255478
2213	1	-0.16932891399660832
2213	2	-0.7744980012461937
2213	3	-0.18972592835693547
2213	4	-0.08224205243062244
2213	5	0.0045467172946871415
2213	6	0.049922957591114425
2213	7	-0.08478023642329191
2213	8	0.04417376402369655
2213	9	-0.23417338300815516
2213	10	-1.378475185407602
2213	11	-0.44416187068612345
2213	12	-0.11190833256752342
2213	13	-0.23677745750906354
2213	14	-0.011194463899094842
2213	15	-0.028693614069773332
2213	16	-0.08777540830181607
2213	17	-0.13136553311040444
2213	18	0.04273895056012833
2213	19	0.07558723876488926
2213	20	-0.041697361237972075
2213	21	0.008848669808955378
2213	22	0.009663719042073637
2213	23	-0.8239173546961069
2213	24	-1.3853791287088129
2213	25	-1.5877940543016322
2214	1	-0.11882269652408439
2214	2	-0.2145384516285489
2214	3	-0.0674505003186745
2214	4	-0.13442787543498014
2214	5	-0.12658629304251434
2214	6	-0.16951536594056194
2214	7	-0.061948466033065586
2214	8	-0.021264047039593947
2214	9	-0.33631316292883895
2214	10	-0.06981838044499256
2214	11	-0.17516353695636866
2214	12	-0.21662827756799885
2214	13	-0.18140514110278616
2214	14	-0.14515532524775665
2214	15	-0.2384326505479919
2214	16	-0.3639015832339041
2214	17	-0.3169872258674431
2214	18	-0.22138229971741907
2214	19	-0.9669346015138061
2214	20	-0.5525529077679892
2214	21	-0.39277408977135053
2214	22	-0.7380562988323405
2214	23	-1.8906926270491198
2214	24	-1.4019087773986953
2214	25	-1.6651973825252426
2215	1	-0.505022914270782
2215	2	-0.2918577269893845
2215	3	-1.269439998648032
2215	4	-0.9968529183529213
2215	5	-0.3524946943790255
2215	6	-0.35891271441377626
2215	7	-0.17340051739125073
2215	8	-0.30238531454380535
2215	9	-0.38224410685053556
2215	10	-1.3368188770303249
2215	11	-0.5781584926049845
2215	12	-0.5840675525192793
2215	13	-0.6022645835620253
2215	14	-0.9106732756984428
2215	15	-1.1957834360401876
2215	16	-0.7523984228351352
2215	17	-0.6314001886218321
2215	18	-0.4587443309050272
2215	19	-1.083069017437296
2215	20	-1.4287188594200302
2215	21	-0.6907380472322515
2215	22	-0.48739503829057185
2215	23	-0.7483333006304349
2215	24	-1.709002908476748
2215	25	-0.8656299749054739
2216	1	-0.517466674304405
2216	2	-0.3656871088837299
2216	3	-1.4539282752418679
2216	4	-0.6538811055140172
2216	5	-0.5539802735214892
2216	6	-0.5408510726546112
2216	7	-0.2930167248366331
2216	8	-0.5088115704261753
2216	9	-0.21300667945435237
2216	10	-0.36644872675851303
2216	11	-0.4561274116263192
2216	12	-0.3764556065647682
2216	13	-1.0430127215282283
2216	14	-0.6308884861547668
2216	15	-1.0347126029652358
2216	16	-0.7444785781366718
2216	17	-1.7152153942551323
2216	18	-0.6741862065122106
2216	19	-0.5408564872072432
2216	20	-0.4923415056094953
2216	21	-0.30323150169251534
2216	22	-0.32572709696616897
2216	23	-0.23352401698808478
2216	24	-0.43345437502215134
2216	25	-0.38977784898306567
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3754.0
3	392.0
4	468.0
5	456.0
6	324.0
7	225.0
8	289.0
9	604.0
10	1264.0
11	2252.0
12	4122.0
13	6294.0
14	8808.0
15	11822.0
16	13685.0
17	14838.0
18	15141.0
19	15319.0
20	15948.0
21	17183.0
22	17921.0
23	19309.0
24	20438.0
25	21649.0
26	22882.0
27	25797.0
28	28839.0
29	33994.0
30	41849.0
31	52593.0
32	70783.0
33	100420.0
34	145423.0
35	232774.0
36	426695.0
37	1495794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97360350842608	20.68134711789017	13.035124520527596	23.309924853156154
2	14.719288875086262	21.515126412463403	39.838842002704226	23.926742709746115
3	18.561258820531755	26.414153757620195	30.10065502902477	24.923932392823282
4	12.141219042150455	18.265902728222798	37.83124977028449	31.761628459342262
5	12.23832455575999	39.54739282660984	35.235901980199735	12.978380637430439
6	30.7229059757357	38.13711824211542	17.58765104523982	13.552324736909066
7	25.50713062805891	33.81173588962042	22.95614718340105	17.724986298919617
8	24.061392087958573	38.04804643413769	20.877331978773164	17.01322949913057
9	23.994387131872237	17.755532340043835	21.741022375538602	36.50905815254533
10	14.48116300658132	31.311302196051205	33.83813602139146	20.369398775976006
11	31.351467159221148	26.485619613985385	22.65462875716662	19.508284469626847
12	20.409062558613705	28.926195182209103	31.3144620748307	19.350280184346495
13	26.66060556255403	24.10263238467345	26.147076089395043	23.089685963377477
14	19.811844080559997	24.77032635188312	27.975182972704044	27.442646594852842
15	23.673902650998695	30.630161224511728	23.634659924683085	22.06127619980649
16	20.586877116493465	31.764840068670765	26.945024558951193	20.70325825588458
17	20.5320586556949	31.090929639652572	28.35166237128592	20.025349333366616
18	20.59848095086873	30.043100280262763	29.377354879775456	19.98106388909305
19	20.82713192404032	30.10662437054179	29.573069155897624	19.49317454952027
20	21.784133325521598	29.267972220267364	28.76019786802027	20.18769658619077
21	21.236990889245803	29.664117855670764	28.79166113443987	20.307230120643567
22	20.756277030013607	29.494561496260218	28.28306220261568	21.466099271110494
23	20.151397751048293	29.90024112007294	28.814836771591168	21.133524357287598
24	20.678977778070585	30.17569981202884	28.39238364841591	20.75293876148467
25	21.25592039110988	28.805589265282062	27.362608786103117	22.57588155750494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8835.0
1	8835.0
2	8043.0
3	7251.0
4	7251.0
5	7251.0
6	8861.0
7	10471.0
8	10471.0
9	10471.0
10	13768.5
11	17066.0
12	17066.0
13	17066.0
14	25363.0
15	33660.0
16	33660.0
17	33660.0
18	49717.5
19	65775.0
20	65775.0
21	65775.0
22	89795.5
23	113816.0
24	113816.0
25	113816.0
26	144721.5
27	175627.0
28	175627.0
29	175627.0
30	206816.0
31	238005.0
32	238005.0
33	238005.0
34	262440.0
35	286875.0
36	286875.0
37	286875.0
38	300937.5
39	315000.0
40	315000.0
41	315000.0
42	316432.5
43	317865.0
44	317865.0
45	317865.0
46	353818.5
47	389772.0
48	389772.0
49	389772.0
50	353581.0
51	317390.0
52	317390.0
53	317390.0
54	272034.5
55	226679.0
56	226679.0
57	226679.0
58	193605.5
59	160532.0
60	160532.0
61	160532.0
62	132630.5
63	104729.0
64	104729.0
65	104729.0
66	80968.0
67	57207.0
68	57207.0
69	57207.0
70	42151.5
71	27096.0
72	27096.0
73	27096.0
74	19063.0
75	11030.0
76	11030.0
77	11030.0
78	7521.5
79	4013.0
80	4013.0
81	4013.0
82	2604.0
83	1195.0
84	1195.0
85	1195.0
86	765.5
87	336.0
88	336.0
89	336.0
90	215.5
91	95.0
92	95.0
93	95.0
94	56.0
95	17.0
96	17.0
97	17.0
98	14.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31331867304559863
2	0.1809124714394253
3	0.19869579718428368
4	0.21955833691998336
5	0.244019059296666
6	0.1971734891438678
7	0.19163782354235545
8	0.21668671038919882
9	0.2166175145691799
10	0.2276888457722046
11	0.20046029059476575
12	0.20748366632668455
13	0.22090765541035196
14	0.20890218063707205
15	0.19848820972422695
16	0.2027437526553896
17	0.20253616519533288
18	0.20454284397588113
19	0.2049234209859851
20	0.20063328014481302
21	0.19841901390420807
22	0.20146362998503986
23	0.20388548368570153
24	0.22125363451044647
25	0.21616774173905703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2890348.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.98220157550595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.25863568838545	68.71326284917939
2	8.271003841920674	12.73440169981043
3	1.391908026519501	3.2145643281626675
4	0.41390329845734897	1.2745274861844176
5	0.18754622066416546	0.7218860481946554
6	0.09570449490320265	0.44205256309921975
7	0.07219043287323303	0.38901649186891807
8	0.05235860407054385	0.3224544490216571
9	0.03639357443860575	0.25214917351373356
>10	0.19371818541063732	2.7385528676406556
>50	0.015303992099678117	0.8099455918912788
>100	0.008486247965448342	1.356828604947912
>500	8.587373578679599E-4	0.4904132132037945
>1k	0.0014914912001848621	2.639359029561124
>5k	3.615736242872393E-4	1.846277881629248
>10k+	1.3559010910771475E-4	2.0543077220909787
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	24635	0.8523195130828536	No Hit
CCCATGTACTCTGCGTTGATACCAC	21970	0.7601160829076637	No Hit
GAGTACATGGGAAGCAGTGGTATCA	12438	0.43032880469756585	No Hit
CATGTACTCTGCGTTGATACCACTG	9128	0.3158097225662792	No Hit
GCGTTGATACCACTGCTTCCCATGT	7323	0.2533604949992181	No Hit
GCTTCCCATGTACTCTGCGTTGATA	7181	0.24844759177787587	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	6513	0.22533618789156185	No Hit
GTATCAACGCAGAGTACATGGGAAG	6118	0.21167001343782824	No Hit
GGTATCAACGCAGAGTACATGGGAA	5858	0.2026745568353707	No Hit
TATCAACGCAGAGTACATGGGAAGC	5748	0.19886878673433095	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5195	0.1797361424991039	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4598	0.15908119022346098	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4287	0.14832124021052134	No Hit
GTGGTATCAACGCAGAGTACATGGG	3800	0.13147205803591816	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3543	0.12258039516348897	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3359	0.11621437972174978	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3049	0.10548902761881959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.4597910009452146E-5	0.0
8	0.0	0.0	0.0	3.4597910009452146E-5	0.0
9	3.4597910009452146E-5	0.0	0.0	3.4597910009452146E-5	0.0
10	3.4597910009452146E-5	0.0	0.0	3.4597910009452146E-5	0.0
11	3.4597910009452146E-5	0.0	0.0	3.4597910009452146E-5	0.0
12	3.4597910009452146E-5	0.0	0.0	6.919582001890429E-5	0.0
13	3.4597910009452146E-5	0.0	0.0	6.919582001890429E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAT	60	2.5516008E-5	14.260723	1
TATTCCG	135	5.6639692E-8	10.559284	5
TATACTG	680	0.0	10.062376	5
CTAGTAC	245	0.0	9.696629	3
TTAGACA	335	0.0	9.645365	4
TAGGCCG	80	0.004504283	9.503357	5
CTAGCAC	310	0.0	9.502697	3
TCTATAC	365	0.0	9.372523	3
TTCCGTA	95	0.0018249123	8.999745	7
GTCTTGC	530	0.0	8.969008	1
ATACTGT	800	0.0	8.905536	6
TACACAG	685	0.0	8.879048	5
GTTCTAG	355	0.0	8.837631	1
GTGTAAG	415	0.0	8.70534	1
CACACCG	110	7.121933E-4	8.639415	5
ACACCGT	110	7.152714E-4	8.635671	6
TAGACAG	345	0.0	8.539248	5
CTTATAC	345	0.0	8.538655	3
CCAAGAC	370	0.0	8.475378	3
AGTACTG	360	0.0	8.447428	5
>>END_MODULE