FastQCFastQC Report
Wed 25 May 2016
SRR1295037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890348
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC246440.8526308942729387No Hit
GTACATGGGAAGCAGTGGTATCAAC238790.8261634931157079No Hit
GAGTACATGGGAAGCAGTGGTATCA133200.46084416132590267No Hit
GTATCAACGCAGAGTACTTTTTTTT124980.432404679298133No Hit
GGTATCAACGCAGAGTACTTTTTTT123310.42662682832655446No Hit
CATGTACTCTGCGTTGATACCACTG101050.34961188064551396No Hit
TATCAACGCAGAGTACTTTTTTTTT90660.31366465214569317No Hit
ACGCAGAGTACATGGGAAGCAGTGG83650.2894115172290672No Hit
GCTTCCCATGTACTCTGCGTTGATA82410.28512137638789514No Hit
GCGTTGATACCACTGCTTCCCATGT76800.26571194887259253No Hit
TATCAACGCAGAGTACATGGGAAGC67140.23229036780346174No Hit
GTATCAACGCAGAGTACATGGGAAG65670.22720447503207225No Hit
GGTATCAACGCAGAGTACATGGGAA65550.22678930011195883No Hit
ACTCTGCGTTGATACCACTGCTTCC59780.20682630603650495No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54280.18779745553130625No Hit
GTGGTATCAACGCAGAGTACATGGG39680.13728450691750613No Hit
ACATGGGAAGCAGTGGTATCAACGC36280.1255212175142924No Hit
CAGTGGTATCAACGCAGAGTACATG35560.12303016799361186No Hit
CATGGGAAGCAGTGGTATCAACGCA32260.11161285769049263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG456.7683216E-414.7767695
TATACCG602.571423E-514.2490275
TTAGACT2850.011.3325594
ACCGTGT1502.1373125E-910.7659318
CTAAGAC3200.010.3899153
CTAGACA3050.010.2779874
GCCCTAG2053.6379788E-1210.2087311
TAGACAG5000.09.49935155
GTAGGAC1804.1909516E-99.49935153
CTTAGAC2401.8189894E-129.49935153
CCGTCCA800.00452062359.4991879
GGAACGA900.00111499599.499186515
CTAGGAC2600.09.1339923
CCTACAC5000.09.1193773
TAGGACT3350.09.0740074
GTCTAGG3050.09.0448441
GTACACG850.00735704278.9531121
CCTAGAC2356.548362E-118.893013
TAGGTTG2800.08.8208265
GTATTAG3350.08.8027791
Produced by FastQC (version 0.11.5)