FastQCFastQC Report
Wed 25 May 2016
SRR1295036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25834
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA15736.088875125803205No Hit
AAACAAAAAAAAAAAAAAAAAAAAA1340.518696291708601No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1110.42966633119145314No Hit
CCCATGTACTCTGCGTTGATACCAC1100.4257954633428815No Hit
GGTATCAACGCAGAGTACTTTTTTT990.3832159170085933No Hit
GTATCAACGCAGAGTACTTTTTTTT940.3638615777657351No Hit
AAAAACAAAAAAAAAAAAAAAAAAA740.2864442207943021No Hit
TATCAACGCAGAGTACTTTTTTTTT700.27096074940001547No Hit
ACGCAGAGTACTTTTTTTTTTTTTT400.154834713942866No Hit
GTACATGGGAAGCAGTGGTATCAAC390.15096384609429433No Hit
GTACTGGTTCACTATCGGTCAGTCA380.1470929782457227No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA360.1393512425485794No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA360.1393512425485794No Hit
ATACAGGGTGACAGCCCCGTACACA340.13160950685143608No Hit
ACACAAAAAAAAAAAAAAAAAAAAA300.11612603545714949No Hit
ACACAAAAAACAAAAAAAAAAAAAA280.1083842997600062No Hit
GAACAAAAAAAAAAAAAAAAAAAAA270.10451343191143454No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)