##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295036_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25834 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.676859952001237 27.0 15.0 33.0 15.0 33.0 2 23.82015947975536 27.0 15.0 33.0 15.0 33.0 3 23.908376558024308 27.0 15.0 33.0 15.0 33.0 4 26.015870558179145 27.0 15.0 37.0 15.0 37.0 5 26.549005186962916 33.0 15.0 37.0 15.0 37.0 6 26.411395834946195 33.0 15.0 37.0 15.0 37.0 7 26.452388325462568 33.0 15.0 37.0 15.0 37.0 8 26.40535728110242 33.0 15.0 37.0 15.0 37.0 9 26.341875048385848 33.0 15.0 37.0 15.0 37.0 10 26.463613842223427 33.0 15.0 37.0 15.0 37.0 11 26.489858326236742 33.0 15.0 37.0 15.0 37.0 12 26.602577997987147 33.0 15.0 37.0 15.0 37.0 13 26.312920956878532 33.0 15.0 37.0 6.0 37.0 14 26.7265618951769 33.0 15.0 37.0 6.0 40.0 15 26.69764651234807 33.0 15.0 37.0 6.0 40.0 16 26.71796856855307 33.0 15.0 37.0 6.0 40.0 17 26.565378957962377 33.0 15.0 37.0 6.0 40.0 18 26.5289928001858 27.0 15.0 37.0 6.0 40.0 19 26.464155763722225 27.0 15.0 37.0 6.0 40.0 20 26.22880699852907 27.0 15.0 37.0 6.0 40.0 21 26.124564527367035 27.0 15.0 37.0 6.0 40.0 22 26.061740342184716 27.0 15.0 37.0 6.0 40.0 23 25.877332197878765 27.0 15.0 37.0 6.0 40.0 24 25.773051018038245 27.0 15.0 37.0 6.0 40.0 25 24.19954323759387 27.0 15.0 37.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.05495641731448586 1101 2 -0.22306444570125805 1101 3 -1.148560146959742 1101 4 -0.279659591718719 1101 5 -2.5938361978019273 1101 6 -1.845651991125905 1101 7 -1.3295695614157417 1101 8 -0.6417627350339572 1101 9 1.4939243807570932 1101 10 -0.7739084654173176 1101 11 0.4376482008525002 1101 12 0.5646603852854852 1101 13 -0.6031272560834502 1101 14 1.4755432064777807 1101 15 -0.8995858803247003 1101 16 -1.0826072794829358 1101 17 -1.7580057793626551 1101 18 0.03613615815317672 1101 19 1.0260205161943112 1101 20 -1.0193888810514729 1101 21 -0.34209858592495124 1101 22 -1.8875396091297532 1101 23 0.9113827177711826 1101 24 -1.4297182426205737 1101 25 -0.14319458866141588 1102 1 0.45162708926902084 1102 2 -0.19950242213923275 1102 3 -0.6800916784912729 1102 4 0.19573888367975556 1102 5 -0.42613003009575934 1102 6 1.3816522361783221 1102 7 2.747353515507335 1102 8 3.0172809240097003 1102 9 1.8896277764604896 1102 10 0.5559598644510118 1102 11 -0.1548673916630925 1102 12 1.255582076207176 1102 13 2.000476347520152 1102 14 0.5968183277528993 1102 15 1.6714466907078709 1102 16 1.5306983338226772 1102 17 2.0306290092721326 1102 18 3.321652443669464 1102 19 2.1687786589524514 1102 20 0.6971738355112436 1102 21 2.237249993423628 1102 22 3.102758381168236 1102 23 2.0707728771613425 1102 24 1.8509470380447048 1102 25 2.2767638312970035 1103 1 -2.448006610364679 1103 2 -2.7064621290989415 1103 3 -1.4134250118246037 1103 4 -1.837960750019878 1103 5 -2.066423070388801 1103 6 -0.4989338444077589 1103 7 -1.7009981328443118 1103 8 -0.9189828122540362 1103 9 -2.289859403026689 1103 10 -1.1414760329848868 1103 11 -3.6164058532015524 1103 12 -1.9195094988843984 1103 13 -1.0757141286703238 1103 14 -0.345306214371643 1103 15 0.12126354052472266 1103 16 -1.4802906771663338 1103 17 -3.665341686698561 1103 18 -1.0248677028506847 1103 19 -3.161624271450478 1103 20 -0.989273050935644 1103 21 -2.0162298600562245 1103 22 -1.162443084033228 1103 23 -3.894794888406423 1103 24 -4.085316698219032 1103 25 -1.8397196851865125 1104 1 -0.7253585267409832 1104 2 -0.7636049862417984 1104 3 -0.6963518410928984 1104 4 -0.3020722482777174 1104 5 -2.0796634885072685 1104 6 -0.5351707782056678 1104 7 -0.8905451711718406 1104 8 -1.607484554414313 1104 9 -2.119127695709615 1104 10 -0.19513456957025355 1104 11 0.651886829725278 1104 12 -0.41358615393666653 1104 13 0.9598259410160885 1104 14 -0.4644699774378438 1104 15 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10 1.1085239670151132 2209 11 -0.2164058532015538 2209 12 1.4376333582584557 2209 13 0.54214301418682 2209 14 -0.034591928657356164 2209 15 0.5355492548104372 2209 16 0.6018521799765253 2209 17 0.14537259901572241 2209 18 -0.19629627427925556 2209 19 -1.240195700021907 2209 20 -0.5214159080785024 2209 21 0.8801987113723477 2209 22 0.6482712016810588 2209 23 0.8444908258792907 2209 24 0.49325473035239753 2209 25 -0.354005399472225 2210 1 0.9662791039210354 2210 2 2.5697283470915337 2210 3 1.4627654643658694 2210 4 0.10965829759917156 2210 5 2.7954816915159633 2210 6 0.5391613936874791 2210 7 0.6466209147747328 2210 8 0.852445759174536 2210 9 0.6625215493542633 2210 10 1.8490001574913038 2210 11 2.502641765846068 2210 12 2.9185857392108367 2210 13 1.4052382522820572 2210 14 2.1023128332474066 2210 15 2.8307873500485314 2210 16 3.4958997990241443 2210 17 2.763229741872866 2210 18 3.675132297149318 2210 19 5.166947157120951 2210 20 3.953584091921499 2210 21 4.193293949467584 2210 22 3.18517596358582 2210 23 2.138538444926912 2210 24 2.4718261589238253 2210 25 0.9507565052896787 2211 1 0.36508862773056094 2211 2 -0.6802716529084663 2211 3 0.5282416548420628 2211 4 2.178110678551551 2211 5 -0.427732594198325 2211 6 -1.4965528920268056 2211 7 -1.9754028947490738 2211 8 0.8345886163173937 2211 9 0.6684739303066429 2211 10 -3.320642699651554 2211 11 -0.6372391865348845 2211 12 -0.1290333084082107 2211 13 -1.2286903191465122 2211 14 0.11124140467597599 2211 15 -1.0561174118562313 2211 16 -1.852314486690144 2211 17 0.5578725990157238 2211 18 0.145370392387413 2211 19 -0.44019570002190633 2211 20 -0.8589159080785009 2211 21 -2.732301288627653 2211 22 -3.1808954649856105 2211 23 -3.0221758407873764 2211 24 -1.6859119363142696 2211 25 -1.6206720661388943 2212 1 3.119340328410832 2212 2 1.9098644015133033 2212 3 4.292697437154988 2212 4 2.174284147939307 2212 5 2.1254136643050785 2212 6 3.056168196408567 2212 7 2.160226356951604 2212 8 4.076935555092906 2212 9 1.6080997806467785 2212 10 1.5768913139538903 2212 11 2.6284921059821222 2212 12 4.6804905011155995 2212 13 3.4018368917378403 2212 14 3.483265214199786 2212 15 3.3171819078716602 2212 16 3.193178710588768 2212 17 2.212209333709602 2212 18 2.84860168490442 2212 19 4.564906340794419 2212 20 3.0964412347786414 2212 21 2.4654027930050013 2212 22 2.4946997731096303 2212 23 1.5501030707772507 2212 24 3.7065200364748456 2212 25 3.4337497025685906 2213 1 -0.009911372269439056 2213 2 0.03639501375820231 2213 3 -0.05786945626904938 2213 4 0.9239440118848847 2213 5 1.3653229613572329 2213 6 2.7423359968620815 2213 7 1.6926526608064805 2213 8 2.1540330607618365 2213 9 1.5101405969733115 2213 10 0.969635078126224 2213 11 1.9169274801317826 2213 12 2.804300024925123 2213 13 1.2449207919645993 2213 14 0.895963626898201 2213 15 1.7688825881437715 2213 16 2.4657410688654124 2213 17 1.953705932349056 2213 18 -0.6629629409459206 2213 19 1.2764709666447587 2213 20 1.6646952030326112 2213 21 1.3774209335945677 2213 22 3.1232712016810567 2213 23 1.0639352703237357 2213 24 -1.012300825203159 2213 25 1.4459946005277722 2214 1 1.3016828306291117 2214 2 1.3885689268016783 2214 3 1.1604880316536565 2214 4 0.9906106785515512 2214 5 1.1764340724683429 2214 6 1.2172152239152219 2214 7 1.6052130472799107 2214 8 0.03567557283913203 2214 9 0.7318797274080922 2214 10 1.6367848365803326 2214 11 -1.0077102010276384 2214 12 0.7767637930410665 2214 13 -0.8763352466827463 2214 14 -3.724809319961704 2214 15 -1.4166246582330402 2214 16 -0.40575651567565174 2214 17 0.09863346858094246 2214 18 -1.0049919264531688 2214 19 -1.9293261348045156 2214 20 0.17097539626932345 2214 21 0.34650305919843305 2214 22 -1.5332505374493763 2214 23 -2.8718135219467946 2214 24 -1.0654409218215157 2214 25 -2.171396703820051 2215 1 1.5798322174741486 2215 2 0.8004975778607673 2215 3 0.42247242407282926 2215 4 1.2726619606028322 2215 5 3.2918186878529596 2215 6 0.9201137746398587 2215 7 1.516584284738105 2215 8 3.4788193855481637 2215 9 -0.11037222353951037 2215 10 0.8123701208612673 2215 11 3.02461978782409 2215 12 1.665838486463585 2215 13 0.8209891680329733 2215 14 -0.40318167224710066 2215 15 -0.04650202724084451 2215 16 -0.08468628156193603 2215 17 0.3896033682464939 2215 18 1.501139623156643 2215 19 0.9123684025421959 2215 20 1.5433276816650903 2215 21 0.673147429321066 2215 22 1.3078865862964406 2215 23 0.19897800536647026 2215 24 0.6971008841985515 2215 25 2.302404856938029 2216 1 -1.0001074507008134 2216 2 -1.1753696921241499 2216 3 -1.5722485412363696 2216 4 -0.7740952038013909 2216 5 -1.0000365157669506 2216 6 -2.472043088105238 2216 7 -1.4472166202392707 2216 8 1.1213533221997452 2216 9 0.2983758910909593 2216 10 0.07028867289746543 2216 11 -0.2634646767309654 2216 12 0.21410394649375064 2216 13 -0.068151103460238 2216 14 -1.8948860463044142 2216 15 -0.7350389804836794 2216 16 -1.8878537023764181 2216 17 -1.1443332833372182 2216 18 -2.6904139213380773 2216 19 -2.778430994139555 2216 20 -2.075827672784385 2216 21 -0.89774246509824 2216 22 -1.1943758571424716 2216 23 -1.1275679976501216 2216 24 -1.1038040931770148 2216 25 -1.2187112818251684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17.0 3 9.0 4 3.0 5 2.0 6 1.0 7 8.0 8 35.0 9 102.0 10 177.0 11 352.0 12 573.0 13 665.0 14 788.0 15 961.0 16 1043.0 17 995.0 18 1114.0 19 1010.0 20 993.0 21 972.0 22 1001.0 23 860.0 24 808.0 25 734.0 26 671.0 27 655.0 28 676.0 29 689.0 30 693.0 31 703.0 32 750.0 33 878.0 34 948.0 35 1114.0 36 2338.0 37 2496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.382198952879584 28.966453364359126 10.036843125848362 23.614504556912934 2 15.81170089112747 30.430065865943433 30.37969779155366 23.378535451375434 3 19.617873890632872 33.2829515947758 23.69491919544239 23.404255319148938 4 13.415248653048566 27.14833908291019 29.443001666731266 29.99341059730997 5 14.517754690649712 43.460226391688636 27.279423166382383 14.74259575127927 6 28.97399123997054 41.38144889336796 14.837784410248458 14.80677545641304 7 24.984496124031008 40.25581395348837 18.51937984496124 16.24031007751938 8 23.628610195774375 42.39193642178717 16.359759643341736 17.61969373909672 9 24.4679613908594 26.774431135403344 17.870294995542118 30.88731247819514 10 16.5787841191067 37.36429900744417 26.783498759305214 19.27341811414392 11 28.894832732488275 34.461371477303565 18.176532154901732 18.46726363530643 12 20.733361758207682 36.540175975813014 25.318810806620412 17.40765145935889 13 26.081563033028377 33.04000620251202 21.23585051946038 19.642580244999223 14 20.906976744186046 33.08527131782946 22.705426356589147 23.30232558139535 15 24.782945736434108 37.02713178294574 19.395348837209305 18.794573643410853 16 21.61627906976744 38.05038759689923 21.550387596899228 18.782945736434108 17 21.70070927483431 37.304755629626754 22.44099065927677 18.553544436262158 18 20.38996782571617 36.94228011009032 23.576384850951662 19.09136721324185 19 20.507751937984498 36.2984496124031 25.74418604651163 17.449612403100776 20 22.171401992325286 34.87344470715919 24.15985115702159 18.795302143493934 21 22.79369016704779 35.029650013565366 24.448664780434868 17.72799503895198 22 22.16063260717885 35.33607256376463 24.040623304132104 18.462671524924414 23 20.745794247616097 36.34777889758896 24.091014807349406 18.815412047445538 24 23.27970536925761 33.921302578018995 24.295406086450864 18.503585966272535 25 20.708636997984183 35.20313226856877 24.600713288881998 19.48751744456505 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1644.0 1 1644.0 2 1136.5 3 629.0 4 629.0 5 629.0 6 522.0 7 415.0 8 415.0 9 415.0 10 342.0 11 269.0 12 269.0 13 269.0 14 255.5 15 242.0 16 242.0 17 242.0 18 304.0 19 366.0 20 366.0 21 366.0 22 512.5 23 659.0 24 659.0 25 659.0 26 844.0 27 1029.0 28 1029.0 29 1029.0 30 1277.0 31 1525.0 32 1525.0 33 1525.0 34 1686.5 35 1848.0 36 1848.0 37 1848.0 38 1995.5 39 2143.0 40 2143.0 41 2143.0 42 2311.5 43 2480.0 44 2480.0 45 2480.0 46 2649.5 47 2819.0 48 2819.0 49 2819.0 50 2864.0 51 2909.0 52 2909.0 53 2909.0 54 2560.5 55 2212.0 56 2212.0 57 2212.0 58 2001.0 59 1790.0 60 1790.0 61 1790.0 62 1526.5 63 1263.0 64 1263.0 65 1263.0 66 1027.5 67 792.0 68 792.0 69 792.0 70 608.0 71 424.0 72 424.0 73 424.0 74 320.5 75 217.0 76 217.0 77 217.0 78 157.0 79 97.0 80 97.0 81 97.0 82 66.0 83 35.0 84 35.0 85 35.0 86 26.5 87 18.0 88 18.0 89 18.0 90 12.0 91 6.0 92 6.0 93 6.0 94 4.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18967252458001083 2 0.0929008283657196 3 0.11999690330572114 4 0.13548037470000773 5 0.1470929782457227 6 0.13548037470000773 7 0.13160950685143608 8 0.15096384609429433 9 0.14322211039715105 10 0.16257644964000928 11 0.14322211039715105 12 0.13548037470000773 13 0.1470929782457227 14 0.13160950685143608 15 0.13160950685143608 16 0.13160950685143608 17 0.12773863900286445 18 0.14322211039715105 19 0.13160950685143608 20 0.13548037470000773 21 0.12773863900286445 22 0.1393512425485794 23 0.1393512425485794 24 0.15096384609429433 25 0.1470929782457227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 25834.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.82751412866764 #Duplication Level Percentage of deduplicated Percentage of total 1 97.49722940524566 81.72950375474181 2 1.1590321388991502 1.943175659982968 3 0.4525304765422978 1.138035147480065 4 0.20317694865164387 0.6812727413486103 5 0.18008865903213891 0.7548192304714717 6 0.06464721093461397 0.32515289928001856 7 0.09235315847801995 0.5419214988000309 8 0.05079423716291097 0.34063637067430513 9 0.05541189508681198 0.41805372764573817 >10 0.2077946065755449 3.3599132925601918 >50 0.01847063169560399 1.304482464968646 >100 0.013852973771702994 1.3741580862429357 >500 0.0 0.0 >1k 0.004617657923900997 6.088875125803205 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 1573 6.088875125803205 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 134 0.518696291708601 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 111 0.42966633119145314 No Hit CCCATGTACTCTGCGTTGATACCAC 110 0.4257954633428815 No Hit GGTATCAACGCAGAGTACTTTTTTT 99 0.3832159170085933 No Hit GTATCAACGCAGAGTACTTTTTTTT 94 0.3638615777657351 No Hit AAAAACAAAAAAAAAAAAAAAAAAA 74 0.2864442207943021 No Hit TATCAACGCAGAGTACTTTTTTTTT 70 0.27096074940001547 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 40 0.154834713942866 No Hit GTACATGGGAAGCAGTGGTATCAAC 39 0.15096384609429433 No Hit GTACTGGTTCACTATCGGTCAGTCA 38 0.1470929782457227 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 36 0.1393512425485794 No Hit CAAAAAAAAAAAAAAAAAAAAAAAA 36 0.1393512425485794 No Hit ATACAGGGTGACAGCCCCGTACACA 34 0.13160950685143608 No Hit ACACAAAAAAAAAAAAAAAAAAAAA 30 0.11612603545714949 No Hit ACACAAAAAACAAAAAAAAAAAAAA 28 0.1083842997600062 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 27 0.10451343191143454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0038708678485716496 0.0 12 0.0 0.0 0.0 0.0038708678485716496 0.0 13 0.0 0.0 0.0 0.0038708678485716496 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE