FastQCFastQC Report
Wed 25 May 2016
SRR1295036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25834
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6292.4347758767515675No Hit
GGTATCAACGCAGAGTACTTTTTTT5662.190911202291554No Hit
TATCAACGCAGAGTACTTTTTTTTT4981.9276921885886815No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3041.1767438259657816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1660.6425640628628939No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1470.5690175737400325No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1430.553534102345746No Hit
CCCATGTACTCTGCGTTGATACCAC1380.5341797631028876No Hit
GTACATGGGAAGCAGTGGTATCAAC1170.452891538282883No Hit
GTGGTATCAACGCAGAGTACTTTTT1120.4335371990400248No Hit
GCAGAGTACTTTTTTTTTTTTTTTT970.37547418131145005No Hit
GAGTACATGGGAAGCAGTGGTATCA620.2399938066114423No Hit
ATCAACGCAGAGTACTTTTTTTTTT600.23225207091429897No Hit
CATGTACTCTGCGTTGATACCACTG570.22063946736858403No Hit
AACGCAGAGTACTTTTTTTTTTTTT400.154834713942866No Hit
ACGCAGAGTACATGGGAAGCAGTGG380.1470929782457227No Hit
CAACGCAGAGTACTTTTTTTTTTTT370.14322211039715105No Hit
ATACAGGGTGACAGCCCCGTACACA350.13548037470000773No Hit
GCTTCCCATGTACTCTGCGTTGATA340.13160950685143608No Hit
GCGTTGATACCACTGCTTCCCATGT300.11612603545714949No Hit
GTACTGGTTCACTATCGGTCAGTCA290.11225516760857783No Hit
TATCAACGCAGAGTACATGGGAAGC280.1083842997600062No Hit
GGTATCAACGCAGAGTACATGGGAA280.1083842997600062No Hit
TCAACGCAGAGTACTTTTTTTTTTT260.1006425640628629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCAG250.00587625119.0000025
GGTATCA1800.011.6111111
GTATCAA2801.9099389E-107.80357172
AACGCAG3253.2421667E-86.4307697
TCAACGC3253.2421667E-86.4307695
ATCAACG3253.2421667E-86.4307694
TATCAAC3451.5266778E-86.33333353
CAACGCA3304.3894033E-86.33333356
TACTTTT3501.4009856E-75.971428416
GTACTTT3602.4320252E-75.805555315
CTTTTTT3602.4320252E-75.805555318
AGTACTT3456.9893576E-75.782608514
GAGTACT3509.141004E-75.713
ACTTTTT3755.381953E-75.573333317
ACGCAGA3702.5601275E-65.3918927
CGCAGAG3753.2778898E-65.328
AGAGTAC3804.1805124E-65.2512
CAGAGTA3855.3114236E-65.18181811
GCAGAGT3855.3114236E-65.18181810
Produced by FastQC (version 0.11.5)