##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295036_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25834 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.7116590539599 33.0 27.0 33.0 27.0 33.0 2 30.660873267786638 33.0 27.0 33.0 27.0 33.0 3 30.892041495703335 33.0 33.0 33.0 27.0 33.0 4 34.45591081520477 37.0 33.0 37.0 27.0 37.0 5 34.66996980723078 37.0 33.0 37.0 27.0 37.0 6 33.58926221258806 37.0 33.0 37.0 22.0 37.0 7 33.2707284973291 37.0 33.0 37.0 22.0 37.0 8 33.272586513896414 37.0 33.0 37.0 22.0 37.0 9 33.14972516838275 37.0 33.0 37.0 15.0 37.0 10 33.12317101494155 37.0 33.0 37.0 15.0 37.0 11 33.07157234652009 37.0 33.0 37.0 15.0 37.0 12 33.08043663389332 37.0 33.0 37.0 15.0 37.0 13 32.89831230161802 37.0 33.0 37.0 15.0 37.0 14 33.87524192924054 37.0 33.0 40.0 15.0 40.0 15 33.92072462646125 37.0 33.0 40.0 15.0 40.0 16 33.98621971045908 37.0 33.0 40.0 15.0 40.0 17 34.0195091739568 37.0 33.0 40.0 15.0 40.0 18 34.06352094139506 37.0 33.0 40.0 15.0 40.0 19 34.035263606100486 37.0 33.0 40.0 15.0 40.0 20 33.95889138344817 37.0 33.0 40.0 15.0 40.0 21 33.9848261980336 37.0 33.0 40.0 15.0 40.0 22 33.95997522644577 37.0 33.0 40.0 15.0 40.0 23 33.899280018580164 37.0 33.0 40.0 15.0 40.0 24 33.85604242471162 37.0 33.0 40.0 15.0 40.0 25 32.15282186266161 37.0 27.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8058972713368533 1101 2 -0.1247916955375814 1101 3 -0.028532559706427207 1101 4 1.382404135270967 1101 5 0.01997566540973139 1101 6 0.7766080152202974 1101 7 0.40833695440569073 1101 8 1.2388640284921593 1101 9 1.6909050342660024 1101 10 2.3329497867622138 1101 11 0.5797346777822199 1101 12 1.3568161718903156 1101 13 1.499709046728185 1101 14 0.5732148472003189 1101 15 1.6276320556257886 1101 16 0.9299506077314419 1101 17 1.6314194230689907 1101 18 1.0034419897255944 1101 19 1.2304891924028638 1101 20 0.2406752226093758 1101 21 1.5297678878006238 1101 22 2.124732623594525 1101 23 -0.6781336989768931 1101 24 -0.34475626868200493 1101 25 0.9256315917500224 1102 1 0.7525362179757984 1102 2 0.4143628436169564 1102 3 0.8557363245624572 1102 4 0.13708188994872472 1102 5 0.49676014219421205 1102 6 0.6400868786991651 1102 7 1.2725088185775562 1102 8 1.3691481587762908 1102 9 1.3104476538086232 1102 10 2.286518740331168 1102 11 1.4196515176990587 1102 12 0.008236823310966201 1102 13 0.5613861084052445 1102 14 1.154642428627902 1102 15 1.6338690618627965 1102 16 1.68324236102319 1102 17 0.6577534494030175 1102 18 1.0748210611046716 1102 19 1.53402349593717 1102 20 -0.019893037958887305 1102 21 1.5845149425476777 1102 22 1.6049821038440015 1102 23 1.098027077183886 1102 24 2.1015361776104413 1102 25 0.27698294310137683 1103 1 1.4082138736534588 1103 2 1.5315789608330768 1103 3 0.8718535406796732 1103 4 1.112906065772897 1103 5 1.2315586769927407 1103 6 1.0715887102009916 1103 7 2.102545448614187 1103 8 0.4651188547469829 1103 9 1.4862718296327984 1103 10 1.4132586670710978 1103 11 3.2723987704463156 1103 12 1.7738045888787326 1103 13 1.973840320859459 1103 14 2.125338399323873 1103 15 2.849253677247411 1103 16 2.2619969397777737 1103 17 2.1735043651539385 1103 18 2.7239053101889183 1103 19 3.263693825607497 1103 20 2.7881655700997214 1103 21 3.2401925982253346 1103 22 2.246740345602248 1103 23 2.6570014361582466 1103 24 3.157946434020694 1103 25 3.3912686573870907 1104 1 -0.5151310740817365 1104 2 0.1935998667564185 1104 3 -0.49260638963391656 1104 4 -1.352599160707939 1104 5 -0.4632148421710198 1104 6 -0.8733590249906413 1104 7 -1.1915312064380785 1104 8 -1.6596198212112014 1104 9 -0.032891933433404574 1104 10 -1.5114800088870908 1104 11 -1.0418869438394012 1104 12 -1.6721884424801523 1104 13 -0.28539312861789057 1104 14 0.19154048991620698 1104 15 0.05413172602789729 1104 16 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1107 15 0.5349679629616944 1107 16 0.6667588445396788 1107 17 1.6449329365825065 1107 18 1.3477148339984382 1107 19 2.197027158940834 1107 20 2.25483223676639 1107 21 1.668764026796758 1107 22 2.4419784408403373 1107 23 2.218906198063003 1107 24 2.1436607197349815 1107 25 0.5626972288156615 1108 1 -0.013713036645548016 1108 2 0.4817450737898845 1108 3 0.14494324167635142 1108 4 -0.4013796485128154 1108 5 -0.1943549442697119 1108 6 -0.19950763531395665 1108 7 -0.7984512291266768 1108 8 0.29103247600944115 1108 9 1.330125650230805 1108 10 -1.2352463163176068 1108 11 -0.32095671128126213 1108 12 0.8269607350581367 1108 13 -0.12316964591794743 1108 14 -1.4494124312409156 1108 15 0.22599786329391947 1108 16 0.46797700622295224 1108 17 0.656560843559248 1108 18 0.5697524862686549 1108 19 0.19591973922875638 1108 20 0.02005925780736817 1108 21 0.2368703390891156 1108 22 -0.01040251154061167 1108 23 1.3174665524373168 1108 24 0.4044580619276701 1108 25 1.0676806175199793 1109 1 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4.346740345602242 2210 23 3.8379538171106233 2210 24 3.2388988149730764 2210 25 3.2055543716728074 2211 1 0.515356730796313 2211 2 -0.465444848690737 2211 3 0.7772106835368149 2211 4 -0.2972129818461511 2211 5 -0.6202270372929704 2211 6 0.6561125197248003 2211 7 0.09302163909038086 2211 8 -0.05392876430063609 2211 9 0.45948611534708306 2211 10 0.15671104802347458 2211 11 0.27061305616059883 2211 12 -0.1279811254069827 2211 13 0.6607450827642225 2211 14 0.015219351704821804 2211 15 0.8742536772474097 2211 16 -0.08919353641270789 2211 17 -0.3342337300841578 2211 18 -0.6254994517158465 2211 19 0.5452414446551188 2211 20 -0.6945725251383692 2211 21 1.2223354553681887 2211 22 0.7812641551260526 2211 23 -0.688831897175092 2211 24 0.5871131006873611 2211 25 -1.889683723565291 2212 1 0.9184179552861096 2212 2 1.2866810016493986 2212 3 1.255527010067425 2212 4 0.761885657609632 2212 5 0.7866607178090703 2212 6 1.8797519755071121 2212 7 1.127035244532557 2212 8 1.8814453853592283 2212 9 1.1597412173878965 2212 10 1.7438709119690543 2212 11 1.7948477500381514 2212 12 2.3248249970419934 2212 13 1.916697463716602 2212 14 2.696766970752442 2212 15 1.9227230650025149 2212 16 2.282405103043075 2212 17 1.8184023243376117 2212 18 1.630027759168513 2212 19 2.2718570909136204 2212 20 2.975920672140539 2212 21 2.668764026796758 2212 22 2.3569444272349003 2212 23 2.5182259259541624 2212 24 1.9905994952451849 2212 25 1.8484115145299462 2213 1 0.2875789530185351 2213 2 0.07761070686482086 2213 3 0.1258217946479263 2213 4 -1.1347129818461497 2213 5 0.6283840738181397 2213 6 -0.8522208136085325 2213 7 0.04857719464593657 2213 8 0.8016267912549253 2213 9 0.17198611534708164 2213 10 -1.0724556186431897 2213 11 -0.41966472161718116 2213 12 -0.296036680962537 2213 13 0.2024117494308868 2213 14 -0.9000584260729525 2213 15 -0.32852410053036607 2213 16 -1.3475268697460407 2213 17 -0.7439559523063792 2213 18 0.5620005482841535 2213 19 -0.717258555344884 2213 20 0.07070525263940652 2213 21 0.3417798998126358 2213 22 0.32293082179272403 2213 23 0.9458903250471309 2213 24 0.26905754513180824 2213 25 0.6769829431013754 2214 1 -1.4955128344210777 2214 2 -1.1982347037631982 2214 3 -1.5244197512457944 2214 4 -1.2926839963389014 2214 5 -1.4870748633799238 2214 6 -1.7116411034636023 2214 7 -1.7185725638081735 2214 8 -2.1118997787933864 2214 9 -2.641057362913788 2214 10 -1.2463686621214514 2214 11 -0.2592782481872291 2214 12 -3.0274376471461117 2214 13 -1.7975882505691132 2214 14 -2.067208184527061 2214 15 -2.3377028444917194 2214 16 -0.7939036813402396 2214 17 -2.00724097646097 2214 18 -3.995970466208597 2214 19 -2.3723310191129983 2214 20 -2.036058032384748 2214 21 -0.9678819359361626 2214 22 -2.2930112071927873 2214 23 -2.912563781233061 2214 24 -2.055097044240174 2214 25 -1.8751909699420999 2215 1 -0.6320791666395849 2215 2 -1.6369192076650947 2215 3 -1.6570841882580574 2215 4 -1.196251443384611 2215 5 -0.24682960139553245 2215 6 -0.7445285059162217 2215 7 -0.18902964296090374 2215 8 -2.246236456608326 2215 9 -1.510065166704198 2215 10 -1.3032248494124197 2215 11 -2.1957330976855545 2215 12 -2.4020195869454426 2215 13 -1.3616908146716789 2215 14 -1.6915114175259447 2215 15 -2.058438630444897 2215 16 -3.9065012287203977 2215 17 -2.8037850121354424 2215 18 -2.9508199645363575 2215 19 -6.030079068165396 2215 20 -5.071175089240935 2215 21 -3.7744594164266836 2215 22 -3.446299947438046 2215 23 -4.260947281790475 2215 24 -2.590771514697252 2215 25 -1.5948119286934954 2216 1 -2.1464079750860385 2216 2 -2.140689946729953 2216 3 -1.255877551757301 2216 4 -1.6072620014539893 2216 5 -2.0062564490576733 2216 6 -2.2973188528242154 2216 7 -2.6520764001253063 2216 8 -2.779418960379065 2216 9 -3.4338962375940945 2216 10 -3.0430438539373057 2216 11 -2.894828120309988 2216 12 -2.116951713642276 2216 13 -1.9446470740985262 2216 14 -3.9418884914324295 2216 15 -2.9860404403996483 2216 16 -2.6749778501381947 2216 17 -5.5021258869469065 2216 18 -2.4281955301472173 2216 19 -2.6741213004429234 2216 20 -3.105111740824647 2216 21 -2.7556057211023983 2216 22 -2.5398142762464957 2216 23 -4.71946915207705 2216 24 -2.406759448332245 2216 25 -3.7818405863103877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 4.0 10 10.0 11 19.0 12 57.0 13 98.0 14 187.0 15 239.0 16 267.0 17 290.0 18 315.0 19 296.0 20 278.0 21 334.0 22 364.0 23 373.0 24 376.0 25 358.0 26 364.0 27 313.0 28 352.0 29 459.0 30 518.0 31 636.0 32 775.0 33 1077.0 34 1448.0 35 2321.0 36 4086.0 37 9618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99717174847933 20.653984735190424 12.599279377009804 23.749564139320444 2 15.084772005883721 22.08330107610126 37.52806379190214 25.303863126112873 3 17.589223503909576 27.12704188279012 31.3075791592475 23.9761554540528 4 11.256483703646357 18.37888054501819 36.60292637609352 33.76170937524193 5 10.850042579546335 39.60671982658512 34.48169079507626 15.061546798792289 6 29.646976852210265 39.20414957033367 15.99829681814663 15.150576759309436 7 23.991638925447084 35.7590771851049 22.22652318649841 18.0227607029496 8 25.13354494077572 36.61840984748781 19.818843384686847 18.429201827049624 9 24.088410621661378 17.024076798018115 21.901370287218395 36.98614229310211 10 16.30022451033522 31.288224820004647 30.881783695904623 21.529766973755514 11 30.804366338933185 26.329643105984363 21.46783308817837 21.39815746690408 12 21.661376480606954 30.154060540373152 30.026321901370288 18.15824107764961 13 27.97089107377874 23.995509793295657 26.73221336223581 21.301385770689787 14 20.825269025315478 24.773554230858558 29.51536734535883 24.88580939846714 15 24.293566617635673 29.105055353410236 26.558024309050086 20.043353719904005 16 19.009832004335372 30.932104977936053 28.98505845010451 21.07300456762406 17 19.110474568398235 29.790198962607416 30.277928311527447 20.821398157466902 18 18.37888054501819 26.98381977239297 33.9165440891848 20.720755593404043 19 19.218858868158243 26.666408608810094 35.80165673143919 18.313075791592475 20 20.097545869784007 25.652241232484325 35.325539986064875 18.924672911666796 21 19.741426027715413 26.488348687775797 35.02748316172486 18.74274212278393 22 19.315630564372533 26.410931330804367 34.590075094836266 19.68336300998684 23 19.18789192536967 26.39157699156151 35.04683750096771 19.373693582101108 24 19.791747309746846 25.505148254238602 35.6235968104049 19.07950762560966 25 19.741426027715413 24.955485019741428 34.66362158395913 20.639467368584036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 180.0 1 180.0 2 143.0 3 106.0 4 106.0 5 106.0 6 174.0 7 242.0 8 242.0 9 242.0 10 256.5 11 271.0 12 271.0 13 271.0 14 251.0 15 231.0 16 231.0 17 231.0 18 382.0 19 533.0 20 533.0 21 533.0 22 807.5 23 1082.0 24 1082.0 25 1082.0 26 1466.5 27 1851.0 28 1851.0 29 1851.0 30 2096.0 31 2341.0 32 2341.0 33 2341.0 34 2495.0 35 2649.0 36 2649.0 37 2649.0 38 2595.0 39 2541.0 40 2541.0 41 2541.0 42 2542.5 43 2544.0 44 2544.0 45 2544.0 46 2774.0 47 3004.0 48 3004.0 49 3004.0 50 2844.0 51 2684.0 52 2684.0 53 2684.0 54 2376.5 55 2069.0 56 2069.0 57 2069.0 58 1766.5 59 1464.0 60 1464.0 61 1464.0 62 1248.0 63 1032.0 64 1032.0 65 1032.0 66 780.0 67 528.0 68 528.0 69 528.0 70 409.0 71 290.0 72 290.0 73 290.0 74 206.5 75 123.0 76 123.0 77 123.0 78 85.0 79 47.0 80 47.0 81 47.0 82 31.5 83 16.0 84 16.0 85 16.0 86 11.0 87 6.0 88 6.0 89 6.0 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08902996051714794 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 25834.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.59146860726175 #Duplication Level Percentage of deduplicated Percentage of total 1 97.20461095100865 78.33862351939305 2 1.3208453410182517 2.1289773167144075 3 0.49471661863592703 1.1960981652086398 4 0.297790585975024 0.9599752264457692 5 0.13928914505283382 0.5612758380428893 6 0.12007684918347744 0.5806301772857475 7 0.06243996157540826 0.3522489742200201 8 0.052833813640730067 0.34063637067430513 9 0.014409221902017292 0.10451343191143454 >10 0.22574447646493753 3.4528141209259116 >50 0.01921229586935639 1.0683595262057752 >100 0.03842459173871278 6.2901602539289305 >500 0.009606147934678195 4.6256870790431215 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 629 2.4347758767515675 No Hit GGTATCAACGCAGAGTACTTTTTTT 566 2.190911202291554 No Hit TATCAACGCAGAGTACTTTTTTTTT 498 1.9276921885886815 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 304 1.1767438259657816 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 166 0.6425640628628939 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 147 0.5690175737400325 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 143 0.553534102345746 No Hit CCCATGTACTCTGCGTTGATACCAC 138 0.5341797631028876 No Hit GTACATGGGAAGCAGTGGTATCAAC 117 0.452891538282883 No Hit GTGGTATCAACGCAGAGTACTTTTT 112 0.4335371990400248 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 97 0.37547418131145005 No Hit GAGTACATGGGAAGCAGTGGTATCA 62 0.2399938066114423 No Hit ATCAACGCAGAGTACTTTTTTTTTT 60 0.23225207091429897 No Hit CATGTACTCTGCGTTGATACCACTG 57 0.22063946736858403 No Hit AACGCAGAGTACTTTTTTTTTTTTT 40 0.154834713942866 No Hit ACGCAGAGTACATGGGAAGCAGTGG 38 0.1470929782457227 No Hit CAACGCAGAGTACTTTTTTTTTTTT 37 0.14322211039715105 No Hit ATACAGGGTGACAGCCCCGTACACA 35 0.13548037470000773 No Hit GCTTCCCATGTACTCTGCGTTGATA 34 0.13160950685143608 No Hit GCGTTGATACCACTGCTTCCCATGT 30 0.11612603545714949 No Hit GTACTGGTTCACTATCGGTCAGTCA 29 0.11225516760857783 No Hit TATCAACGCAGAGTACATGGGAAGC 28 0.1083842997600062 No Hit GGTATCAACGCAGAGTACATGGGAA 28 0.1083842997600062 No Hit TCAACGCAGAGTACTTTTTTTTTTT 26 0.1006425640628629 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0038708678485716496 0.0 12 0.0 0.0 0.0 0.0038708678485716496 0.0 13 0.0 0.0 0.0 0.0038708678485716496 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGCCAG 25 0.005876251 19.000002 5 GGTATCA 180 0.0 11.611111 1 GTATCAA 280 1.9099389E-10 7.8035717 2 AACGCAG 325 3.2421667E-8 6.430769 7 TCAACGC 325 3.2421667E-8 6.430769 5 ATCAACG 325 3.2421667E-8 6.430769 4 TATCAAC 345 1.5266778E-8 6.3333335 3 CAACGCA 330 4.3894033E-8 6.3333335 6 TACTTTT 350 1.4009856E-7 5.9714284 16 GTACTTT 360 2.4320252E-7 5.8055553 15 CTTTTTT 360 2.4320252E-7 5.8055553 18 AGTACTT 345 6.9893576E-7 5.7826085 14 GAGTACT 350 9.141004E-7 5.7 13 ACTTTTT 375 5.381953E-7 5.5733333 17 ACGCAGA 370 2.5601275E-6 5.391892 7 CGCAGAG 375 3.2778898E-6 5.32 8 AGAGTAC 380 4.1805124E-6 5.25 12 CAGAGTA 385 5.3114236E-6 5.181818 11 GCAGAGT 385 5.3114236E-6 5.181818 10 >>END_MODULE