FastQCFastQC Report
Wed 25 May 2016
SRR1295035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4405711
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC341100.7742223672864608No Hit
CCCATGTACTCTGCGTTGATACCAC305060.6924194528420043No Hit
GAGTACATGGGAAGCAGTGGTATCA166130.3770787507396649No Hit
CATGTACTCTGCGTTGATACCACTG122680.27845675760393723No Hit
GCGTTGATACCACTGCTTCCCATGT101620.23065516553400803No Hit
GCTTCCCATGTACTCTGCGTTGATA96480.2189884901665134No Hit
GTATCAACGCAGAGTACATGGGAAG88130.20003581714733445No Hit
ACGCAGAGTACATGGGAAGCAGTGG87290.19812920093941702No Hit
TATCAACGCAGAGTACATGGGAAGC77960.17695214234433446No Hit
GGTATCAACGCAGAGTACATGGGAA76270.1731162121165006No Hit
ACTCTGCGTTGATACCACTGCTTCC69450.1576363043331712No Hit
GTATCAACGCAGAGTACTTTTTTTT65640.14898843796154582No Hit
GGTATCAACGCAGAGTACTTTTTTT55960.12701695594649764No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN52750.11973095829481326No Hit
GTGGTATCAACGCAGAGTACATGGG47750.10838205229530488No Hit
TATCAACGCAGAGTACTTTTTTTTT45110.10238982992756447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG604.099141E-412.6650299
ACCGTTT658.036492E-411.6909288
TCTACGC600.00585388911.0895973
CTAAGAC4200.010.636963
TAGCGTG856.560445E-410.0650815
TAGGACA5400.09.6824964
CCTACAC4150.09.6198913
AAGACCG800.0044951059.5059095
GTACGAC800.00449721659.5053683
ATCGCTC800.00452262029.4988798
ACCGCGC1502.665256E-79.49671818
CACCGTT1554.2718784E-79.1926737
GGGCCGT1054.5019333E-49.0463466
GTTCTAG5800.09.018641
GTCGTGG950.00180647499.010011
AGCACCG950.00181454279.0055995
GTCCTAC2750.08.9918081
TCGTAGG850.00737175358.951121
TACACCG1502.6820217E-68.8721825
TAGGACT4950.08.8342774
Produced by FastQC (version 0.11.5)