##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295035_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4405711
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263902012637686	33.0	33.0	33.0	27.0	33.0
2	31.134007201107835	33.0	33.0	33.0	27.0	33.0
3	31.24212913647763	33.0	33.0	33.0	27.0	33.0
4	34.82331614579349	37.0	37.0	37.0	33.0	37.0
5	35.05775140493782	37.0	37.0	37.0	33.0	37.0
6	35.074143764763505	37.0	37.0	37.0	33.0	37.0
7	34.91488638269737	37.0	37.0	37.0	33.0	37.0
8	34.855617629027414	37.0	37.0	37.0	33.0	37.0
9	34.75163441269752	37.0	37.0	37.0	27.0	37.0
10	34.667068266620305	37.0	37.0	37.0	27.0	37.0
11	34.860764584876314	37.0	37.0	37.0	33.0	37.0
12	34.773414325179296	37.0	37.0	37.0	27.0	37.0
13	34.69277308475295	37.0	37.0	37.0	27.0	37.0
14	36.104917685249895	40.0	37.0	40.0	27.0	40.0
15	36.26664095761161	40.0	37.0	40.0	27.0	40.0
16	36.34721546647068	40.0	37.0	40.0	27.0	40.0
17	36.277455330138544	40.0	37.0	40.0	27.0	40.0
18	36.21251303138131	40.0	37.0	40.0	27.0	40.0
19	36.30222794913239	40.0	37.0	40.0	27.0	40.0
20	36.30809578749037	40.0	37.0	40.0	27.0	40.0
21	36.30334127681094	40.0	37.0	40.0	27.0	40.0
22	36.213629990709784	40.0	37.0	40.0	27.0	40.0
23	36.121226063171186	40.0	37.0	40.0	27.0	40.0
24	36.18052341608426	40.0	37.0	40.0	27.0	40.0
25	35.05999621854452	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.377149578441351
1101	2	0.35081442817951825
1101	3	0.3808992270805298
1101	4	0.43657570523484424
1101	5	0.29522290124135964
1101	6	0.25988772807002647
1101	7	0.21850411771968936
1101	8	0.40233386569603624
1101	9	0.4387603164296223
1101	10	0.5883358211694016
1101	11	0.4498020813207333
1101	12	0.4217298313278235
1101	13	0.49937921571198274
1101	14	0.9625361814056674
1101	15	0.7190921711903115
1101	16	0.6763558323971566
1101	17	0.6480554809788188
1101	18	0.6455327576077821
1101	19	0.5760210759588986
1101	20	0.5481705454762604
1101	21	0.5687972780533741
1101	22	0.6299368278034976
1101	23	0.7799975012450489
1101	24	0.822023865776238
1101	25	0.785296258170419
1102	1	0.38993011352599183
1102	2	0.44031298825260023
1102	3	0.4405169362320045
1102	4	0.43624482841126166
1102	5	0.3261348178202894
1102	6	0.27824164372428584
1102	7	0.28975487667536726
1102	8	0.45262825694395303
1102	9	0.46655953992744514
1102	10	0.622132047836736
1102	11	0.4690347144652378
1102	12	0.4829829855197687
1102	13	0.5221749020585804
1102	14	0.8057222912634572
1102	15	0.7046196523487112
1102	16	0.7761956857864192
1102	17	0.6867469021337698
1102	18	0.8074028243958793
1102	19	0.6886052573729771
1102	20	0.7196313053788899
1102	21	0.6837209925634937
1102	22	0.676508854786114
1102	23	0.8080437682971819
1102	24	0.8267558499788166
1102	25	0.8627529071791784
1103	1	0.434276537119878
1103	2	0.4051660210975605
1103	3	0.4010739444289335
1103	4	0.40577529014053226
1103	5	0.24000664218060308
1103	6	0.21981209484614794
1103	7	0.16354799733834113
1103	8	0.3111303768232787
1103	9	0.3786758971883728
1103	10	0.5570111503654331
1103	11	0.34702218251361217
1103	12	0.43553964196832595
1103	13	0.431879286867634
1103	14	0.6584733940266503
1103	15	0.6697214280825676
1103	16	0.6096162005023729
1103	17	0.5982553942462232
1103	18	0.5577622672594416
1103	19	0.5644460527639765
1103	20	0.5871805490053958
1103	21	0.5229698815562003
1103	22	0.5379646533442184
1103	23	0.6707617847295069
1103	24	0.6519240869179868
1103	25	0.7414036132636497
1104	1	0.29859527635741756
1104	2	0.19316110547839216
1104	3	0.2996717624886003
1104	4	0.2801084470606483
1104	5	0.16823188855098437
1104	6	0.08940784961217219
1104	7	0.16499225798470718
1104	8	0.35824379779738535
1104	9	0.3182575441363511
1104	10	0.4793020866705291
1104	11	0.35565516570750333
1104	12	0.41672345473470074
1104	13	0.29619683604571634
1104	14	0.6467070741013998
1104	15	0.6005618188801236
1104	16	0.4190341289501589
1104	17	0.5230260102861166
1104	18	0.6190220148006773
1104	19	0.5072097166112499
1104	20	0.4766032781081222
1104	21	0.4629836548103512
1104	22	0.46591369365108193
1104	23	0.5233425409292138
1104	24	0.5718562355658392
1104	25	0.6067341275781573
1105	1	0.17250877449841795
1105	2	0.09220233543202738
1105	3	0.17005673679447142
1105	4	0.2725093277423696
1105	5	0.08754300630521783
1105	6	0.1009060428961206
1105	7	0.11190549583129439
1105	8	0.2617902808106152
1105	9	0.11147749973853394
1105	10	0.3442266751723366
1105	11	0.11222794073015052
1105	12	0.23319315771490068
1105	13	0.1916505496741152
1105	14	0.5039088750204996
1105	15	0.3992967198147994
1105	16	0.32392448134788054
1105	17	0.26843931576377855
1105	18	0.33784255146183995
1105	19	0.3597489511637946
1105	20	0.3707366511486683
1105	21	0.3487498695043669
1105	22	0.35077626615557733
1105	23	0.43299548549559574
1105	24	0.5069157338682402
1105	25	0.4181906055559992
1106	1	0.11234598586542077
1106	2	-0.0627995774901784
1106	3	-0.044721267567751966
1106	4	-0.020264259228724768
1106	5	-0.27240792634748345
1106	6	-0.2247709388561887
1106	7	-0.5161598003859709
1106	8	-0.05209871650661313
1106	9	0.19855534391276564
1106	10	-0.26727981971264114
1106	11	-0.3149041055745059
1106	12	-0.6873881243628688
1106	13	-0.13070946068410905
1106	14	-0.13461470613241033
1106	15	0.07968016128436517
1106	16	0.11821600625902562
1106	17	-0.07105469014651788
1106	18	-0.2716697758964699
1106	19	-0.3816201741117382
1106	20	0.03969206017477944
1106	21	0.06603981716283158
1106	22	0.23321167532453302
1106	23	-0.27475679335686465
1106	24	0.17649923646266075
1106	25	0.40836469933142183
1107	1	-1.0320250863150733
1107	2	-1.1416532448870313
1107	3	-0.7879339851016418
1107	4	-0.9314320413958157
1107	5	-0.9574673413004504
1107	6	-0.8816878815245843
1107	7	-1.0026078456757546
1107	8	-0.8214099658080514
1107	9	-1.0246862826741392
1107	10	-0.9407523437708605
1107	11	-0.8940587244471843
1107	12	-0.9150700452670364
1107	13	-0.8805083003652001
1107	14	-1.1172886107707853
1107	15	-1.0323933392716569
1107	16	-0.9639471617270132
1107	17	-1.164592260045076
1107	18	-1.2991516999660675
1107	19	-1.205569001338361
1107	20	-1.2798119742424987
1107	21	-1.2343287388767976
1107	22	-1.1549836737904897
1107	23	-0.8977932368895338
1107	24	-0.8981150143080612
1107	25	-0.8470584801782906
1108	1	0.04027092059238058
1108	2	-0.2176049864685723
1108	3	-0.04145675491418288
1108	4	-0.19301502228690026
1108	5	-0.45702681580692683
1108	6	-0.012349480256382606
1108	7	0.03282259825396494
1108	8	0.08855229429793354
1108	9	-0.2993171525503442
1108	10	-0.5878420891929395
1108	11	-0.1020631353567154
1108	12	0.03857705181296467
1108	13	0.04671763385534433
1108	14	-0.2937927240357041
1108	15	0.011890966400180503
1108	16	-0.8619223834689649
1108	17	-0.778308275947424
1108	18	-0.621941623188178
1108	19	-0.15300215258837113
1108	20	-0.8922585752263004
1108	21	-0.12163591729113676
1108	22	-0.21796841163704528
1108	23	-0.1427886771577036
1108	24	-0.33150383912978754
1108	25	-1.143966885294894
1109	1	0.16974528182587534
1109	2	0.08370416775366962
1109	3	0.19727223843569774
1109	4	0.2461103169744021
1109	5	0.0797369222958153
1109	6	0.10582321558799634
1109	7	0.17995735562323034
1109	8	0.26887813433334173
1109	9	0.2453776343892642
1109	10	0.3742707675302128
1109	11	0.17282492315067088
1109	12	0.30101477487082917
1109	13	0.20116517220122176
1109	14	0.4833511278905718
1109	15	0.45265206668837976
1109	16	0.3247192370064056
1109	17	0.42767870806439134
1109	18	0.4602129294200097
1109	19	0.4089533239446794
1109	20	0.31908713127095467
1109	21	0.30545844353009954
1109	22	0.4613656412509428
1109	23	0.5256878911039706
1109	24	0.5244028546775183
1109	25	0.3827113107152229
1110	1	0.037380522187419274
1110	2	-0.0646368033026441
1110	3	0.017842483742324333
1110	4	-1.0233404590916564
1110	5	-0.2405634755382735
1110	6	-0.06360981196063165
1110	7	0.03889538489659117
1110	8	0.10110571885478947
1110	9	0.10482500872244316
1110	10	-0.0017326482041823965
1110	11	0.03287786321525488
1110	12	0.052117991210856474
1110	13	0.1103484905996055
1110	14	0.2193106265852478
1110	15	0.13529274377642508
1110	16	0.0703731175276161
1110	17	0.25943043979781777
1110	18	0.270575470135789
1110	19	0.14211582017381374
1110	20	0.17379634357090623
1110	21	0.1784718188001051
1110	22	-0.35783181868243474
1110	23	0.05716147787329362
1110	24	0.2411923634660127
1110	25	0.3243166199642502
1111	1	-0.053238809427345046
1111	2	-0.32693747664578865
1111	3	-0.23626702621120899
1111	4	-0.09216767276152638
1111	5	-0.07527496681534274
1111	6	-0.027277622157811265
1111	7	0.045801344463669125
1111	8	0.1309356506550614
1111	9	0.024920252652023578
1111	10	0.10867652005330086
1111	11	-0.09515170146543994
1111	12	0.07195521959594231
1111	13	-0.026831484376288017
1111	14	-0.13867034887946517
1111	15	-0.08615789209712688
1111	16	-0.08891064742455512
1111	17	-0.039645750115319345
1111	18	-0.014264077908656247
1111	19	-0.08883343246316144
1111	20	-0.06754350895359806
1111	21	-0.11937585114287685
1111	22	-0.05282040741658278
1111	23	0.09204992341781093
1111	24	0.08760135325159268
1111	25	0.07117287526213545
1112	1	-0.12193391052593228
1112	2	-0.43322021862748983
1112	3	-0.1532829837669638
1112	4	-0.16405259478129608
1112	5	-0.05956370732197058
1112	6	-0.08867083256572528
1112	7	-0.0411171345763961
1112	8	-0.0015427646176107146
1112	9	-0.19396124098585688
1112	10	0.10188404463669798
1112	11	-0.08854151232478813
1112	12	-0.04639287739459519
1112	13	-0.11773206448292228
1112	14	-0.3020565187857045
1112	15	-0.19663262187417985
1112	16	-0.11945780651670646
1112	17	-0.281175264603867
1112	18	-0.11137354691213375
1112	19	-0.1733310256758358
1112	20	-1.008312280989962
1112	21	-1.3686551671288925
1112	22	-1.8838738598295635
1112	23	-0.6487079317247577
1112	24	-0.4880043404752499
1112	25	-1.5767340539429
1113	1	-0.1705535748225273
1113	2	-0.046937046137841776
1113	3	-0.14215493661859213
1113	4	-0.4484922698819176
1113	5	-0.24596056539505895
1113	6	-0.1176989084274922
1113	7	-0.20884950625427479
1113	8	-0.04286056351676848
1113	9	-0.17053325077760917
1113	10	-2.985628317091482E-4
1113	11	-0.15025779476727763
1113	12	-0.10150089329889767
1113	13	-0.18986926585143493
1113	14	-0.5149565231657149
1113	15	-0.42009691749562705
1113	16	-0.45152142372333515
1113	17	-0.5018052073230734
1113	18	-0.9237044420401048
1113	19	-1.0194992472459248
1113	20	-1.1957830200922572
1113	21	-1.0109054214127795
1113	22	-0.4910064621879542
1113	23	-2.048310421516902
1113	24	-1.0539027534517587
1113	25	-0.5388036571626955
1114	1	-0.4163187911495463
1114	2	-0.5038216068945154
1114	3	-0.5210043677764027
1114	4	-0.716358962228945
1114	5	-0.8254463066261764
1114	6	-0.5618219920066423
1114	7	-0.5305586230047368
1114	8	-0.29400694823075213
1114	9	-1.2519777522543905
1114	10	-0.39392496857070114
1114	11	-0.46425308691145517
1114	12	-0.46490791219768113
1114	13	-0.5365840041346885
1114	14	-0.9823861116884913
1114	15	-1.5355203730921616
1114	16	-1.324428387118381
1114	17	-1.695856117062604
1114	18	-1.875171894551201
1114	19	-1.7011764659486914
1114	20	-0.9873308913577361
1114	21	-0.8384677445242588
1114	22	-0.6265188945516655
1114	23	-0.5148177181241493
1114	24	-1.1254578164840865
1114	25	-0.8190873050014176
1115	1	-0.6861019012383238
1115	2	-0.48171641835320855
1115	3	-0.5799708351651915
1115	4	-0.6696307731048279
1115	5	-0.3451763283713163
1115	6	-0.38704760512097636
1115	7	-0.31884861459645464
1115	8	-0.2109447168397267
1115	9	-0.5071687285959428
1115	10	-0.25483176078645897
1115	11	-0.46210303748516424
1115	12	-0.4854387226969479
1115	13	-0.4149114086681678
1115	14	-0.5239195826450427
1115	15	-1.5916019496096965
1115	16	-0.8619689688359458
1115	17	-0.7477847605545662
1115	18	-2.305634027917776
1115	19	-1.2069270637101468
1115	20	-0.8829582960507665
1115	21	-0.8461569120633214
1115	22	-0.7827469303178987
1115	23	-0.45636249433370324
1115	24	-0.43849590341039146
1115	25	-0.6003452088965844
1116	1	-1.4815954331761425
1116	2	-0.742463623244479
1116	3	-1.6194981931993802
1116	4	-0.9174152310497234
1116	5	-0.9430041706212862
1116	6	-0.5305948334121027
1116	7	-1.315028098461589
1116	8	-3.102510401588386
1116	9	-1.4825051802747282
1116	10	-0.8187599442609041
1116	11	-0.5927013555683089
1116	12	-0.6969879154546277
1116	13	-1.6185519421147205
1116	14	-1.641633795497519
1116	15	-1.424308426149068
1116	16	-0.9292911816351719
1116	17	-1.5730817512566304
1116	18	-2.4131267443637725
1116	19	-1.2895516582849496
1116	20	-0.8874993734737942
1116	21	-0.6936376973875511
1116	22	-0.47105915999794945
1116	23	-0.5540978581295803
1116	24	-0.5641930385750058
1116	25	-0.5449227662673763
1201	1	0.3523054402125716
1201	2	0.30794136043338227
1201	3	0.3720033578393682
1201	4	0.4743780959869497
1201	5	0.4227636177993688
1201	6	0.1627886912622074
1201	7	0.21099291373502638
1201	8	0.32914680078580716
1201	9	0.2732399185569605
1201	10	0.5866339430392316
1201	11	0.33024667264188423
1201	12	0.41042811431694304
1201	13	0.41361305157875705
1201	14	0.8952939138519582
1201	15	0.7302851215537771
1201	16	0.6582781294300659
1201	17	0.7472514052245671
1201	18	0.6633282978228863
1201	19	0.6131444401474653
1201	20	0.6881055599543728
1201	21	0.6637118948442762
1201	22	0.7389244393472651
1201	23	0.796341243166367
1201	24	0.7689984097444835
1201	25	0.9252838478310395
1202	1	0.35131011746739915
1202	2	0.3795710996241297
1202	3	0.4443319595598183
1202	4	0.5449262065568377
1202	5	0.3068274996693461
1202	6	0.16184989278773543
1202	7	0.16584804469414394
1202	8	0.2980648102974115
1202	9	0.2848091604145466
1202	10	0.471992202656466
1202	11	0.25985609600923
1202	12	0.34595989269205774
1202	13	0.2052653845817929
1202	14	0.6349813435180423
1202	15	0.4849578881040486
1202	16	0.4317878546435665
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2210	21	0.4990504267822544
2210	22	0.5479539209212874
2210	23	0.6563966292052896
2210	24	0.6156020269648224
2210	25	0.6182255564137549
2211	1	0.21892111799955316
2211	2	0.07185556688878236
2211	3	0.06511537748418306
2211	4	0.23216862829186624
2211	5	0.08036100152194336
2211	6	0.12259594685115616
2211	7	0.19015651173406667
2211	8	0.265952441693031
2211	9	0.2886345330267659
2211	10	0.3843794424743052
2211	11	0.20714824848310087
2211	12	0.26317268128355664
2211	13	0.12075615199589862
2211	14	0.11022086119758256
2211	15	0.18649605837666172
2211	16	0.10494716131177739
2211	17	0.20861323948629007
2211	18	0.19074091080683075
2211	19	0.10255889785977956
2211	20	0.14805593050714805
2211	21	0.2502179002286411
2211	22	0.19329550395796247
2211	23	0.33078532602461763
2211	24	0.3920528889858872
2211	25	0.32414745143098855
2212	1	0.05945341716046926
2212	2	0.2025517292575323
2212	3	0.12972343960282728
2212	4	0.10450885401461107
2212	5	0.21222194278227136
2212	6	0.1786427259826695
2212	7	0.14915298072580185
2212	8	0.30641331080928325
2212	9	0.12355000608336297
2212	10	0.26223195117085396
2212	11	0.19286899177854622
2212	12	0.14752508344817272
2212	13	0.07171966110753658
2212	14	0.15748141886533062
2212	15	0.1307747638432133
2212	16	0.0957384016607179
2212	17	0.23791781598212225
2212	18	0.2241329917655932
2212	19	0.21304732712970775
2212	20	0.1967652681431744
2212	21	0.20311421546552566
2212	22	-0.3363487892046493
2212	23	0.2206992170611457
2212	24	0.23622760818678046
2212	25	0.3834726123295198
2213	1	-0.057478116295559545
2213	2	-0.7873645179064859
2213	3	-0.1617034986964896
2213	4	-0.1461216178687721
2213	5	-0.06304538051935538
2213	6	-0.017951212484881296
2213	7	-0.04643261765261286
2213	8	0.13809314619696522
2213	9	-0.23628292317423671
2213	10	-1.4531456834547498
2213	11	-0.3689040085577986
2213	12	-0.12258421242273698
2213	13	-0.07429895335879166
2213	14	0.0281405746122374
2213	15	0.025718529933804746
2213	16	-0.01828553139391431
2213	17	-0.09225034282510336
2213	18	0.06886484454290098
2213	19	0.021734689354936165
2213	20	-0.031169271772668594
2213	21	0.046235032116527464
2213	22	0.1463220465124948
2213	23	-0.889959846809667
2213	24	-1.4480493030887374
2213	25	-1.3127712708252872
2214	1	-0.020503531352463966
2214	2	-0.23929963113251418
2214	3	-0.07070130617478299
2214	4	-0.06094322441303035
2214	5	-0.032777940818881746
2214	6	-0.08643900070909893
2214	7	-0.08576489503274587
2214	8	-0.04373393327185937
2214	9	-0.37894911140256937
2214	10	-0.02197147141386324
2214	11	-0.1586350801194385
2214	12	-0.19195814005697542
2214	13	-0.0780050560779344
2214	14	-0.05122545168625692
2214	15	-0.1759502919621312
2214	16	-0.272599772810743
2214	17	-0.21625834264544608
2214	18	-0.079395653658203
2214	19	-0.765399637089395
2214	20	-0.41952900634076684
2214	21	-0.37552668643158427
2214	22	-0.5857033025797094
2214	23	-1.932672399012148
2214	24	-1.3246284002247037
2214	25	-1.674910516874995
2215	1	-0.4290282034846058
2215	2	-0.3464567635049711
2215	3	-1.2061843781681958
2215	4	-1.00444779047735
2215	5	-0.4539393153956439
2215	6	-0.37519514779111773
2215	7	-0.296845367326263
2215	8	-0.2521950657314065
2215	9	-0.5088066179672239
2215	10	-1.36844018696695
2215	11	-0.7397610342852161
2215	12	-0.6274403112879412
2215	13	-0.6332969774379436
2215	14	-0.841304918275739
2215	15	-1.1434195048134583
2215	16	-0.7442889472100163
2215	17	-0.5991149322560361
2215	18	-0.5425836199581937
2215	19	-1.0830209102469368
2215	20	-1.4118651757495329
2215	21	-0.7773129923113871
2215	22	-0.49429912389297215
2215	23	-0.7561549002647894
2215	24	-1.9133115302868973
2215	25	-1.0055738908404521
2216	1	-0.5852842027017928
2216	2	-0.4273105440436815
2216	3	-1.4099259219090428
2216	4	-0.7721846224396245
2216	5	-0.6046043571652646
2216	6	-0.5519708043362712
2216	7	-0.4499655593167873
2216	8	-0.5149118668111399
2216	9	-0.3746626054481794
2216	10	-0.40067515688719624
2216	11	-0.44225566817641493
2216	12	-0.37812436694208884
2216	13	-1.0539751295517306
2216	14	-0.725198093850949
2216	15	-1.1106045298103524
2216	16	-0.8821788915747462
2216	17	-1.8842426156280467
2216	18	-0.8376170141593207
2216	19	-0.5972461687310684
2216	20	-0.5457113683721673
2216	21	-0.4265377768394032
2216	22	-0.485616889395061
2216	23	-0.3141691793359769
2216	24	-0.4469906893093878
2216	25	-0.4402288801777843
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5838.0
3	591.0
4	721.0
5	650.0
6	490.0
7	332.0
8	511.0
9	1032.0
10	1922.0
11	3771.0
12	6286.0
13	9623.0
14	13141.0
15	17108.0
16	20124.0
17	21635.0
18	22445.0
19	22984.0
20	24088.0
21	25456.0
22	27269.0
23	29175.0
24	30932.0
25	32676.0
26	34242.0
27	38952.0
28	43451.0
29	51227.0
30	62819.0
31	79509.0
32	107009.0
33	151227.0
34	219989.0
35	351866.0
36	649375.0
37	2297245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10268747575097	20.680211515307175	13.074235278902568	23.14286573003929
2	14.897410731828007	21.174539560335607	39.9978533433268	23.93019636450958
3	18.518216947595146	26.357542992178278	29.94250272361505	25.181737336611526
4	11.98644627647958	18.028476923636894	38.039783067627575	31.94529373225595
5	12.37555948455743	39.410649718863986	35.1381104838361	13.075680312742483
6	31.12180231427604	38.14109219328133	17.500191609265382	13.236913883177253
7	25.88952766311352	33.25177268754646	23.08863699266136	17.770062656678654
8	24.078570424447893	37.753260915553994	21.059570652374187	17.10859800762393
9	24.127562196750326	17.329947021584893	21.894825676219767	36.647665105445014
10	14.503301194217416	30.923597293889777	33.97971008821839	20.593391423674415
11	31.643196481792145	26.22282559252399	22.779877292129886	19.35410063355398
12	20.568857712787132	28.56116073357853	31.559252410423678	19.31072914321067
13	26.682050387296236	24.037344042489643	26.275907423790184	23.004698146423934
14	19.938860526713835	24.32695971004241	28.219005778453564	27.51517398479019
15	23.592926154564847	30.502018138800008	23.842671826090953	22.062383880544196
16	20.846871680802042	31.605792433991315	26.83458538881952	20.71275049638712
17	20.4225095842954	30.829703400855536	28.524309630511084	20.22347738433798
18	20.57644346844106	30.000852928900983	29.452931402908828	19.969772199749126
19	20.83493966011149	29.70841387711674	29.693197840096055	19.763448622675718
20	21.92021531979215	29.112886186322857	28.73133674899228	20.235561744892717
21	21.502460046000916	29.573817186729922	28.580178304152344	20.34354446311682
22	20.762364629046914	29.339927650529994	28.520656899526127	21.377050820896965
23	20.164865708278406	29.87841824995428	28.817585978817927	21.139130062949388
24	20.69050314202156	29.852116610584055	28.612483097871287	20.8448971495231
25	21.312865218391185	28.74419580130072	27.521358928030782	22.421580052277314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10246.0
1	10246.0
2	9155.0
3	8064.0
4	8064.0
5	8064.0
6	10809.5
7	13555.0
8	13555.0
9	13555.0
10	18711.0
11	23867.0
12	23867.0
13	23867.0
14	36344.5
15	48822.0
16	48822.0
17	48822.0
18	73852.0
19	98882.0
20	98882.0
21	98882.0
22	136464.0
23	174046.0
24	174046.0
25	174046.0
26	221015.0
27	267984.0
28	267984.0
29	267984.0
30	316013.0
31	364042.0
32	364042.0
33	364042.0
34	402123.0
35	440204.0
36	440204.0
37	440204.0
38	464241.5
39	488279.0
40	488279.0
41	488279.0
42	488878.0
43	489477.0
44	489477.0
45	489477.0
46	536558.0
47	583639.0
48	583639.0
49	583639.0
50	530513.0
51	477387.0
52	477387.0
53	477387.0
54	413281.0
55	349175.0
56	349175.0
57	349175.0
58	299455.5
59	249736.0
60	249736.0
61	249736.0
62	207487.0
63	165238.0
64	165238.0
65	165238.0
66	126702.5
67	88167.0
68	88167.0
69	88167.0
70	64227.0
71	40287.0
72	40287.0
73	40287.0
74	28322.5
75	16358.0
76	16358.0
77	16358.0
78	11014.0
79	5670.0
80	5670.0
81	5670.0
82	3729.5
83	1789.0
84	1789.0
85	1789.0
86	1175.5
87	562.0
88	562.0
89	562.0
90	369.5
91	177.0
92	177.0
93	177.0
94	104.5
95	32.0
96	32.0
97	32.0
98	29.0
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3128439427824476
2	0.18555461309196178
3	0.20384904956316924
4	0.22405010224229413
5	0.25060654228114376
6	0.19842427249540429
7	0.19461104007956945
8	0.21683219802660683
9	0.2174677367625793
10	0.2276363565381388
11	0.20262336771522238
12	0.2083886119629726
13	0.22066812825444065
14	0.20895605726294803
15	0.20060326244730986
16	0.2029411370832086
17	0.2028049502112145
18	0.2064592979430562
19	0.20505203359911717
20	0.20205592241524695
21	0.20069405369530594
22	0.2041895167431545
23	0.2079800513469903
24	0.22277902477034922
25	0.2165371264706196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4405711.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.3150678496724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.66484831714254	64.40504082870014
2	10.10489847311004	15.018924312864474
3	1.881769281848546	4.19531435574012
4	0.5374214440420502	1.597540443114155
5	0.23689017599929071	0.8802254751154063
6	0.13635749692724808	0.6080049981575963
7	0.07726841685017148	0.4019545348600085
8	0.052668431746234194	0.31312464630057874
9	0.04440682101850778	0.29700863252807974
>10	0.23136095146291416	3.134468881255735
>50	0.018281650492173934	0.9424345425245617
>100	0.011153000614028293	1.540602221428066
>500	9.533531104776493E-4	0.4681485804118191
>1k	0.0012916392268749529	2.297067547845752
>5k	2.7677983433028623E-4	1.5310812587212654
>10k+	1.537665746279368E-4	2.3690587404323984
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	34110	0.7742223672864608	No Hit
CCCATGTACTCTGCGTTGATACCAC	30506	0.6924194528420043	No Hit
GAGTACATGGGAAGCAGTGGTATCA	16613	0.3770787507396649	No Hit
CATGTACTCTGCGTTGATACCACTG	12268	0.27845675760393723	No Hit
GCGTTGATACCACTGCTTCCCATGT	10162	0.23065516553400803	No Hit
GCTTCCCATGTACTCTGCGTTGATA	9648	0.2189884901665134	No Hit
GTATCAACGCAGAGTACATGGGAAG	8813	0.20003581714733445	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8729	0.19812920093941702	No Hit
TATCAACGCAGAGTACATGGGAAGC	7796	0.17695214234433446	No Hit
GGTATCAACGCAGAGTACATGGGAA	7627	0.1731162121165006	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6945	0.1576363043331712	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6564	0.14898843796154582	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5596	0.12701695594649764	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5275	0.11973095829481326	No Hit
GTGGTATCAACGCAGAGTACATGGG	4775	0.10838205229530488	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4511	0.10238982992756447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.269781199901673E-5	0.0
10	0.0	0.0	0.0	2.269781199901673E-5	0.0
11	0.0	0.0	0.0	2.269781199901673E-5	0.0
12	0.0	0.0	0.0	4.539562399803346E-5	0.0
13	0.0	0.0	0.0	6.809343599705019E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGATCG	60	4.099141E-4	12.665029	9
ACCGTTT	65	8.036492E-4	11.690928	8
TCTACGC	60	0.005853889	11.089597	3
CTAAGAC	420	0.0	10.63696	3
TAGCGTG	85	6.560445E-4	10.065081	5
TAGGACA	540	0.0	9.682496	4
CCTACAC	415	0.0	9.619891	3
AAGACCG	80	0.004495105	9.505909	5
GTACGAC	80	0.0044972165	9.505368	3
ATCGCTC	80	0.0045226202	9.498879	8
ACCGCGC	150	2.665256E-7	9.496718	18
CACCGTT	155	4.2718784E-7	9.192673	7
GGGCCGT	105	4.5019333E-4	9.046346	6
GTTCTAG	580	0.0	9.01864	1
GTCGTGG	95	0.0018064749	9.01001	1
AGCACCG	95	0.0018145427	9.005599	5
GTCCTAC	275	0.0	8.991808	1
TCGTAGG	85	0.0073717535	8.95112	1
TACACCG	150	2.6820217E-6	8.872182	5
TAGGACT	495	0.0	8.834277	4
>>END_MODULE