FastQCFastQC Report
Wed 25 May 2016
SRR1295035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4405711
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC340140.7720433773345551No Hit
GTACATGGGAAGCAGTGGTATCAAC329850.7486873287875668No Hit
GAGTACATGGGAAGCAGTGGTATCA176230.4000035408586718No Hit
GTATCAACGCAGAGTACTTTTTTTT163510.37113192399592254No Hit
GGTATCAACGCAGAGTACTTTTTTT149890.3402175040532618No Hit
CATGTACTCTGCGTTGATACCACTG138020.31327520121042896No Hit
TATCAACGCAGAGTACTTTTTTTTT117330.2663134281844633No Hit
ACGCAGAGTACATGGGAAGCAGTGG109430.24838215670524008No Hit
GCGTTGATACCACTGCTTCCCATGT108130.2454314411453679No Hit
GCTTCCCATGTACTCTGCGTTGATA106200.24105076342955767No Hit
GTATCAACGCAGAGTACATGGGAAG91270.20716293011502568No Hit
TATCAACGCAGAGTACATGGGAAGC87110.19772064032343473No Hit
GGTATCAACGCAGAGTACATGGGAA83280.18902737832781133No Hit
ACTCTGCGTTGATACCACTGCTTCC81640.1853049371599726No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68210.15482177564529312No Hit
GTGGTATCAACGCAGAGTACATGGG51210.11623549524696468No Hit
ACATGGGAAGCAGTGGTATCAACGC47250.10724716169535406No Hit
CAGTGGTATCAACGCAGAGTACATG45730.10379709427150352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG601.4694469E-615.8321524
TAAGACG658.033584E-411.6914354
TATACTG9700.09.5972215
TATACCG800.00452100869.4992915
AATACCG1002.7594843E-49.499295
GTCTAGG4050.09.3942771
TAGACTG7050.09.16243655
CTACACG950.00182608128.9993294
GTTCTAG3900.08.7800361
GTGTAAG7100.08.7078981
GTCCTAT3850.08.6470041
TCTGCGC1000.0029085578.5493613
TAAGGCT4450.08.5386894
CTTAGAC3450.08.5355953
GGACAGT4900.08.5299756
GACTGTC3800.08.4994627
TCTACAC5600.08.4815093
TCTAGAC3700.08.472343
GTCTAAT3950.08.4280931
GTCTTAG4350.08.3090751
Produced by FastQC (version 0.11.5)