FastQCFastQC Report
Wed 25 May 2016
SRR1295034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4600625
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC357890.7779160440157588No Hit
CCCATGTACTCTGCGTTGATACCAC322720.7014699089797581No Hit
GAGTACATGGGAAGCAGTGGTATCA168590.3664502105692161No Hit
CATGTACTCTGCGTTGATACCACTG123930.26937644341801387No Hit
GCGTTGATACCACTGCTTCCCATGT110510.24020649368292352No Hit
GCTTCCCATGTACTCTGCGTTGATA100780.21905719331612555No Hit
ACGCAGAGTACATGGGAAGCAGTGG91570.1990381741611194No Hit
GTATCAACGCAGAGTACATGGGAAG91040.1978861567721777No Hit
TATCAACGCAGAGTACATGGGAAGC78140.16984648824887924No Hit
GGTATCAACGCAGAGTACATGGGAA73970.1607825023773944No Hit
ACTCTGCGTTGATACCACTGCTTCC72270.15708735226192094No Hit
GTATCAACGCAGAGTACTTTTTTTT62930.13678576280396684No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN53960.11728841190055699No Hit
GTGGTATCAACGCAGAGTACATGGG50120.10894171987501698No Hit
GGTATCAACGCAGAGTACTTTTTTT49800.10824616220622198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG907.4290474E-611.6167542
GGCCGTC600.00589075411.08026411
AGTCCGC700.00149432210.8554376
GTCTAGG3600.010.8320251
CTAGGAC5400.010.2087743
TATACCG1002.7425477E-49.5051365
ACGACAG900.00110889179.5051365
ACACCGT1401.0622043E-69.49850756
GTATAGG3950.09.3906541
AGCACCG1451.6958656E-69.1773725
CTAGAAC6250.09.1245323
ACCGAGT1054.5008078E-49.0465928
GTCCTAC3050.09.043291
GCCCGTC950.00182675718.9989797
GGACCGT950.00182748168.9985866
TAGGACT5450.08.8950044
CTGTGCG1401.0877708E-58.8204279
TAGACAG7600.08.754735
GTACGGA1107.066856E-48.6464061
GCACCGA1107.1602105E-48.6350076
Produced by FastQC (version 0.11.5)