FastQCFastQC Report
Wed 25 May 2016
SRR1295034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4600625
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC366270.7961309604673278No Hit
GTACATGGGAAGCAGTGGTATCAAC342680.7448553185708463No Hit
GAGTACATGGGAAGCAGTGGTATCA180470.39227278902323054No Hit
GTATCAACGCAGAGTACTTTTTTTT158530.34458361635647333No Hit
CATGTACTCTGCGTTGATACCACTG144020.31304442331205No Hit
GGTATCAACGCAGAGTACTTTTTTT141690.3079798940361364No Hit
ACGCAGAGTACATGGGAAGCAGTGG114130.24807498981116696No Hit
GCGTTGATACCACTGCTTCCCATGT113300.24627088710772993No Hit
TATCAACGCAGAGTACTTTTTTTTT110520.24022822986007336No Hit
GCTTCCCATGTACTCTGCGTTGATA105630.22959923923379977No Hit
GTATCAACGCAGAGTACATGGGAAG99240.21570982203504957No Hit
TATCAACGCAGAGTACATGGGAAGC89200.19388670017660645No Hit
GGTATCAACGCAGAGTACATGGGAA83500.18149707920119548No Hit
ACTCTGCGTTGATACCACTGCTTCC83410.18130145360684688No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66840.14528460806955576No Hit
GTGGTATCAACGCAGAGTACATGGG52110.1132672191278359No Hit
CAGTGGTATCAACGCAGAGTACATG49900.10846352397772042No Hit
ACATGGGAAGCAGTGGTATCAACGC47520.1032903138160576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG453.543668E-516.8875856
AATACCG700.001493327310.8563045
CGAATTC750.002650955510.13255115
TGTACCG856.602865E-410.0580465
ACCGTTC1002.759614E-49.4992678
ACCCGTG900.00111517489.4992677
GTATTAG5950.09.1132121
CGTTCCG1151.1124837E-49.08625510
AGGGCCG1054.4991795E-49.0469215
ACGCCGA850.00744409448.9404867
GACCGTC850.00744409448.9404867
AACAGCG1401.0872738E-58.8207477
CTAGCAC4850.08.8137523
GTATAGA6700.08.8029951
TCTACAC6650.08.71361353
GGTGCGT1201.766647E-48.7076628
CGCAAAA1651.0519452E-68.6359782
TCAATAC5200.08.58587553
CTACACT7650.08.5679664
GTAGGCC3000.08.549343
Produced by FastQC (version 0.11.5)