FastQCFastQC Report
Wed 25 May 2016
SRR1295033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095848
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC305920.6003318780308989No Hit
CCCATGTACTCTGCGTTGATACCAC271910.5335912688133555No Hit
GAGTACATGGGAAGCAGTGGTATCA145810.2861349082625698No Hit
CATGTACTCTGCGTTGATACCACTG107310.21058320420860277No Hit
GCGTTGATACCACTGCTTCCCATGT89990.17659474929393498No Hit
GTATCAACGCAGAGTACATGGGAAG80790.15854083559792206No Hit
ACGCAGAGTACATGGGAAGCAGTGG78050.1531639091275878No Hit
GCTTCCCATGTACTCTGCGTTGATA77090.1512800224810473No Hit
GTATCAACGCAGAGTACTTTTTTTT74510.14621707711846976No Hit
TATCAACGCAGAGTACATGGGAAGC69420.1362285531279583No Hit
GGTATCAACGCAGAGTACATGGGAA66890.13126372686155474No Hit
ACTCTGCGTTGATACCACTGCTTCC63760.1251214714410634No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN59730.11721307228944033No Hit
GGTATCAACGCAGAGTACTTTTTTT58980.1157412858468306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT1103.7665814E-812.1033681
TAGTACG803.7592498E-410.6919842
TGTACCG1201.6934266E-59.5041735
TAGACAG8350.09.4472635
GTATTAG6750.09.4393451
TCTGTCG3100.09.1926118
GTACCGT950.0018274548.9986396
ACCGTAT850.0074458298.9402658
GTCCTGG9300.08.7939981
ACACCGA1304.380692E-58.7679056
CTAATAC6300.08.7497013
AGTACGG1107.119588E-48.6399883
GTGTAAG7850.08.6012111
AGGACTG8550.08.5593145
CGTACAC1000.00289573338.5535883
TGTACTG7850.08.4750595
GTGCTAA4400.08.4291311
GTACTAG4300.08.4039991
GTCTTAG5400.08.2770391
GTTCTAA6750.08.1713741
Produced by FastQC (version 0.11.5)