FastQCFastQC Report
Wed 25 May 2016
SRR1295033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095848
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC310650.6096139445289578No Hit
GTACATGGGAAGCAGTGGTATCAAC287570.564322169735047No Hit
GTATCAACGCAGAGTACTTTTTTTT183140.3593906254660657No Hit
GGTATCAACGCAGAGTACTTTTTTT159460.3129214215180673No Hit
GAGTACATGGGAAGCAGTGGTATCA154480.30314875953913856No Hit
TATCAACGCAGAGTACTTTTTTTTT125810.24688726979297657No Hit
CATGTACTCTGCGTTGATACCACTG122650.24068614291478083No Hit
GCGTTGATACCACTGCTTCCCATGT99210.1946879106284175No Hit
ACGCAGAGTACATGGGAAGCAGTGG94780.18599455870740256No Hit
GTATCAACGCAGAGTACATGGGAAG85120.16703794932658902No Hit
GCTTCCCATGTACTCTGCGTTGATA84990.16678283967653665No Hit
TATCAACGCAGAGTACATGGGAAGC76110.14935688819603724No Hit
ACGCAGAGTACTTTTTTTTTTTTTT74260.14572648163759988No Hit
GGTATCAACGCAGAGTACATGGGAA73640.14450980484504247No Hit
ACTCTGCGTTGATACCACTGCTTCC72480.14223344181380607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG3950.010.5958391
ACCGAGT1201.5272472E-610.2909028
GTCTAGG4750.09.6123011
TGTACCG1002.7593947E-49.4993875
AAGGCCG1554.2706233E-79.1929555
CAGTGCG1356.861683E-69.1474689
ATACCGT1356.861683E-69.1474686
TAGACTG6650.09.1422675
TAGACCG1151.11239264E-49.086375
TATACTG12550.09.0830785
TTGCGCT950.00182601598.9994184
CTAGGAC5350.08.7003733
AGACCGT1752.6321504E-78.6850686
GTATTAG7700.08.647431
TACCGTA1107.1546633E-48.6357217
TCTACAC6850.08.5979853
CTAACAC6650.08.5708753
CCAGGAC9550.08.5544213
TAGAACC3000.08.5494484
CCTACAC5700.08.4994523
Produced by FastQC (version 0.11.5)