FastQCFastQC Report
Wed 25 May 2016
SRR1295032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5773397
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC347190.6013617286322074No Hit
CCCATGTACTCTGCGTTGATACCAC325190.5632559132864066No Hit
GAGTACATGGGAAGCAGTGGTATCA160840.27858815182811786No Hit
CATGTACTCTGCGTTGATACCACTG130460.2259674850005984No Hit
GCGTTGATACCACTGCTTCCCATGT104930.18174741837431238No Hit
ATTCCATTCCATTCCATTCCATTCC92790.16071993663349324No Hit
GTATCAACGCAGAGTACTTTTTTTT89380.15481353525489414No Hit
ACGCAGAGTACATGGGAAGCAGTGG86700.1501715541127693No Hit
GTATCAACGCAGAGTACATGGGAAG86460.14975585430899693No Hit
GCTTCCCATGTACTCTGCGTTGATA78170.13539689025369295No Hit
GGTATCAACGCAGAGTACATGGGAA77720.1346174531216197No Hit
TATCAACGCAGAGTACATGGGAAGC76150.13189808357194213No Hit
ACTCTGCGTTGATACCACTGCTTCC73010.12645934447258694No Hit
GGTATCAACGCAGAGTACTTTTTTT70650.12237162973549194No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN68290.11828391499839697No Hit
GAATGGAATGGAATGGAATGGAATG66260.11476778749148898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAG604.0597122E-412.6796741
ACCGTTC600.005882290711.0824828
TGCTCGA600.00588305111.0822910
CGAGCAC600.00588305111.0822910
GTCGTAG700.001479739710.8682931
CTGTCGC5850.010.7170329
TAAGACG2600.010.6017834
AGACGGT3050.010.5886156
GTCTTAC7100.010.4473371
TCTGTCG5850.010.39236458
GACGGTG2750.010.3626617
AAGACGG2950.010.3100215
TATACCG1054.087881E-59.95714955
CCGTTCA1054.1133117E-59.9515319
TGTCGCC6100.09.65482910
CTAGGAC6400.09.6526413
GTCGCCC5950.09.57751711
CGTAGGC800.0045023769.5041393
TGCGCTA1304.2492265E-69.49910510
TCGGAAC1304.2572174E-69.49770413
Produced by FastQC (version 0.11.5)