##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295032_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5773397
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231666556102066	33.0	33.0	33.0	27.0	33.0
2	31.247175622947807	33.0	33.0	33.0	27.0	33.0
3	31.241456979313913	33.0	33.0	33.0	27.0	33.0
4	34.871193683718616	37.0	37.0	37.0	33.0	37.0
5	35.00299788841821	37.0	37.0	37.0	33.0	37.0
6	35.089672163546005	37.0	37.0	37.0	33.0	37.0
7	34.974957724195995	37.0	37.0	37.0	33.0	37.0
8	34.91987767340441	37.0	37.0	37.0	33.0	37.0
9	34.83140341812628	37.0	37.0	37.0	33.0	37.0
10	34.765334862646725	37.0	37.0	37.0	27.0	37.0
11	34.910725695807855	37.0	37.0	37.0	33.0	37.0
12	34.82851447769831	37.0	37.0	37.0	33.0	37.0
13	34.74867569301055	37.0	37.0	37.0	27.0	37.0
14	36.164924047315644	40.0	37.0	40.0	27.0	40.0
15	36.32240343077048	40.0	37.0	40.0	27.0	40.0
16	36.40682010954729	40.0	37.0	40.0	33.0	40.0
17	36.33820937655941	40.0	37.0	40.0	27.0	40.0
18	36.2857546432369	40.0	37.0	40.0	27.0	40.0
19	36.3873000592199	40.0	37.0	40.0	27.0	40.0
20	36.38718608819037	40.0	37.0	40.0	27.0	40.0
21	36.3910351219568	40.0	37.0	40.0	27.0	40.0
22	36.291348576929664	40.0	37.0	40.0	27.0	40.0
23	36.20871542351929	40.0	37.0	40.0	27.0	40.0
24	36.26079169681212	40.0	37.0	40.0	27.0	40.0
25	35.16401660928566	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.33726823422441754
1101	2	0.34142792152031376
1101	3	0.3092862925295208
1101	4	0.423035956370434
1101	5	0.26264978007215944
1101	6	0.20228874739425606
1101	7	0.23361259359906938
1101	8	0.3997363953042594
1101	9	0.3773092359963286
1101	10	0.5486936688112607
1101	11	0.38292304021056367
1101	12	0.46262724452116544
1101	13	0.46126110576008017
1101	14	0.8318065388925504
1101	15	0.6680052639406071
1101	16	0.6372700210199866
1101	17	0.6512412976289639
1101	18	0.6568481984087597
1101	19	0.4908693240135946
1101	20	0.5521535024469131
1101	21	0.49033489489323046
1101	22	0.6034653099386915
1101	23	0.6964762993532432
1101	24	0.6943084798568577
1101	25	0.725093705580214
1102	1	0.38748138804458776
1102	2	0.38578981611069096
1102	3	0.41266832910951123
1102	4	0.4441645823215481
1102	5	0.2593204289982012
1102	6	0.32019933889699814
1102	7	0.32455435513200115
1102	8	0.37800861541229835
1102	9	0.34836023071507327
1102	10	0.582853968602187
1102	11	0.37373665479653084
1102	12	0.43843459256247286
1102	13	0.3634025496509494
1102	14	0.8076826932477204
1102	15	0.6929996387081943
1102	16	0.7515895114506606
1102	17	0.7106343467392335
1102	18	0.7409168636999226
1102	19	0.6385847921404562
1102	20	0.7494686976805625
1102	21	0.7198367872318698
1102	22	0.6937351786418375
1102	23	0.7452490581690725
1102	24	0.7948425345546326
1102	25	0.7843689149403374
1103	1	0.2840186748158473
1103	2	0.3207977213877342
1103	3	0.3248310066903244
1103	4	0.27700643220379817
1103	5	0.11050659993238554
1103	6	0.07504535027859305
1103	7	0.07297445817903991
1103	8	0.2613399637887639
1103	9	0.2826059125531799
1103	10	0.38521186703011523
1103	11	0.23059598151270677
1103	12	0.3356301669080537
1103	13	0.24440916802558377
1103	14	0.6112990814310351
1103	15	0.5350210402015563
1103	16	0.539457983466086
1103	17	0.5173865467556809
1103	18	0.5578676366340005
1103	19	0.5311795751860231
1103	20	0.4930503836690576
1103	21	0.46708216163876415
1103	22	0.5427103786042196
1103	23	0.615484541800086
1103	24	0.5658706053675928
1103	25	0.6281650304678053
1104	1	0.3340464196466364
1104	2	0.3417309294424875
1104	3	0.3443457602708726
1104	4	0.3763631522952693
1104	5	0.2507631355740898
1104	6	0.22450779418200995
1104	7	0.2942225167078263
1104	8	0.37067313812287495
1104	9	0.3484604560093203
1104	10	0.523114867366786
1104	11	0.4327239300012309
1104	12	0.4491070050431105
1104	13	0.45332271513283473
1104	14	0.7174381587980321
1104	15	0.6324712769129803
1104	16	0.4856789641071302
1104	17	0.5603132822615464
1104	18	0.6664582765952005
1104	19	0.5709257660075053
1104	20	0.6091197228032001
1104	21	0.5165190082680127
1104	22	0.5746222695585033
1104	23	0.6028231349130735
1104	24	0.6763577512408432
1104	25	0.7499443891673891
1105	1	0.2507250498411082
1105	2	0.18744485048659953
1105	3	0.15884729740722037
1105	4	0.2732237031498883
1105	5	0.1749622234011099
1105	6	0.15577042490656368
1105	7	0.15646638748813757
1105	8	0.2824305109831329
1105	9	0.3092743100293589
1105	10	0.3805375072317574
1105	11	0.23812316920580656
1105	12	0.21833713716293346
1105	13	0.25766796045877527
1105	14	0.6189489824635572
1105	15	0.4805656568298886
1105	16	0.3861571371107857
1105	17	0.3316897349806922
1105	18	0.4043836437197683
1105	19	0.37963989347556293
1105	20	0.37436674346002974
1105	21	0.4075129353264515
1105	22	0.3354595267873748
1105	23	0.5276899150138021
1105	24	0.502249345538786
1105	25	0.43773783185161363
1106	1	0.04445950054164527
1106	2	-0.16918501462541968
1106	3	-0.12417792201106792
1106	4	-0.05366618066837958
1106	5	-0.46405322289753315
1106	6	-0.3644397258770482
1106	7	-0.7204219180687446
1106	8	-0.238865286708986
1106	9	0.04620205328453153
1106	10	-0.3475436243080239
1106	11	-0.4110589061057439
1106	12	-0.7542961243219324
1106	13	-0.24541150434565395
1106	14	-0.4609101732619223
1106	15	-0.17638847107424027
1106	16	-0.11007576986175138
1106	17	-0.2773467218869925
1106	18	-0.47404853253772217
1106	19	-0.6240114841668287
1106	20	-0.15721666425802283
1106	21	-0.03478561430556226
1106	22	0.2060205453407633
1106	23	-0.6239784927824559
1106	24	0.006595215271545385
1106	25	0.1851079574926331
1107	1	-1.0810946250124793
1107	2	-1.0330253014047734
1107	3	-0.8068354899732952
1107	4	-0.9315271445508486
1107	5	-1.0372690398409503
1107	6	-0.9673175653140262
1107	7	-1.0164752678855393
1107	8	-0.8762835549516268
1107	9	-1.0009305888301867
1107	10	-0.8220370629591685
1107	11	-0.8343413795674266
1107	12	-0.791389541343662
1107	13	-0.8697019494246234
1107	14	-1.1496398655758426
1107	15	-0.9294943675493172
1107	16	-0.973494231846523
1107	17	-1.0881882854167273
1107	18	-1.181608627194592
1107	19	-1.1710997911117644
1107	20	-1.2482037086500881
1107	21	-1.1477383007349502
1107	22	-1.1533731545547283
1107	23	-0.9753416326760558
1107	24	-0.8145346703744707
1107	25	-0.8104308480820137
1108	1	0.0807460810031948
1108	2	-0.10591480223325789
1108	3	-0.03350367926358899
1108	4	-0.1013931271784898
1108	5	-0.42333885923929415
1108	6	-0.030327919395553238
1108	7	-0.014860870176150343
1108	8	0.23021086594056328
1108	9	-0.23029170491475526
1108	10	-0.5104255139673199
1108	11	-0.10794479145821612
1108	12	0.11875083337974246
1108	13	0.1265494027407854
1108	14	-0.25126511818035624
1108	15	0.07837286641387209
1108	16	-0.6949845735428184
1108	17	-0.6206469747976087
1108	18	-0.5390261356515111
1108	19	-0.002356352037253373
1108	20	-0.869453814441357
1108	21	-0.15742062874892326
1108	22	-0.2769786907005667
1108	23	-0.12258186650981173
1108	24	-0.4387820229674233
1108	25	-1.0932631847736474
1109	1	0.08249088956784334
1109	2	0.06095053477357837
1109	3	0.15241818171069355
1109	4	0.19970796887861297
1109	5	0.12163199162111482
1109	6	0.09739597319564552
1109	7	0.18944460816121023
1109	8	0.16567397591641964
1109	9	0.26794498849646686
1109	10	0.22160850254096687
1109	11	0.23335101245935874
1109	12	0.2746163554604806
1109	13	0.20162908941890834
1109	14	0.5166628389933905
1109	15	0.4025289163073964
1109	16	0.3281652587667381
1109	17	0.3373026611238146
1109	18	0.37118876362183073
1109	19	0.3363181179545478
1109	20	0.3028539690776242
1109	21	0.3200784683601725
1109	22	0.3311033442431892
1109	23	0.37468613923272187
1109	24	0.37835999764995876
1109	25	0.3694180583984732
1110	1	0.05679654056476835
1110	2	0.08814022100780505
1110	3	0.14171578968696608
1110	4	-0.8370588786773396
1110	5	-0.19405890629373346
1110	6	0.06503409916768987
1110	7	0.17589486109056196
1110	8	0.2980705220278992
1110	9	0.20806599397583625
1110	10	0.12734682678132714
1110	11	0.1425881404944107
1110	12	0.22818960428998025
1110	13	0.2682029901462144
1110	14	0.3702133446310114
1110	15	0.26395168442558514
1110	16	0.2644348672853809
1110	17	0.34147390437040315
1110	18	0.45058666041545337
1110	19	0.29358774572523316
1110	20	0.38132591444311004
1110	21	0.28844337011585
1110	22	-0.2695148763235835
1110	23	0.22611946234743385
1110	24	0.2772143036021717
1110	25	0.380007333210294
1111	1	-0.032121195757358834
1111	2	-0.2623096789677142
1111	3	-0.12712358999535311
1111	4	-0.14980613630402217
1111	5	-0.15408738044646242
1111	6	-0.11560039786916576
1111	7	-0.057890456014341396
1111	8	0.012133493481073288
1111	9	-0.048880409471713904
1111	10	-0.057554555127680374
1111	11	-0.2328799807664339
1111	12	0.005438407675207202
1111	13	-0.1540575099930166
1111	14	-0.3094126701060844
1111	15	-0.13225328666191416
1111	16	-0.1811117583216415
1111	17	-0.03610365704102492
1111	18	-0.1799533227223904
1111	19	-0.1662455628616044
1111	20	-0.18567657061434062
1111	21	-0.2121582833865432
1111	22	-0.09592696174077275
1111	23	0.006489608153614768
1111	24	-0.003243545703064399
1111	25	-0.04991460556264116
1112	1	-0.0772573476325995
1112	2	-0.3762349071953466
1112	3	-0.09917829900768282
1112	4	-0.21083346346853205
1112	5	-0.04110742160658987
1112	6	-0.12255180873960114
1112	7	-0.08356488579727994
1112	8	0.007287253382514791
1112	9	0.04985352149552824
1112	10	0.24708904229050432
1112	11	0.017397468683626016
1112	12	0.11787679667204998
1112	13	0.030081704967898304
1112	14	-0.1950359627905769
1112	15	-0.1900860147876955
1112	16	-0.10439401197400144
1112	17	-0.19690355631436063
1112	18	-0.10555435383090384
1112	19	-0.07159620182242321
1112	20	-0.8298841861950379
1112	21	-1.1615266638008421
1112	22	-1.582226878376062
1112	23	-0.5057453725964933
1112	24	-0.23486339115162025
1112	25	-1.3989620086111856
1113	1	-0.14734429360991186
1113	2	-0.1714735821046247
1113	3	-0.22414898252930726
1113	4	-0.5403775489502891
1113	5	-0.21861205936771455
1113	6	-0.18886196196236682
1113	7	-0.12809843771364626
1113	8	-0.015224041980481218
1113	9	-0.18875738895012972
1113	10	-0.09548448469060844
1113	11	-0.13230809752273132
1113	12	-0.17762335694081344
1113	13	-0.2638729199386276
1113	14	-0.5325484120125523
1113	15	-0.5074451770155761
1113	16	-0.4695859168189429
1113	17	-0.4285020121330021
1113	18	-0.8593493592273305
1113	19	-0.9348079029082399
1113	20	-1.2041302415718107
1113	21	-0.9828577486366541
1113	22	-0.47681351720598286
1113	23	-1.9926336804564073
1113	24	-1.0210020118381422
1113	25	-0.42955548693437606
1114	1	-0.3625893619004401
1114	2	-0.43979372492448476
1114	3	-0.32588073284680874
1114	4	-0.5524006343498371
1114	5	-0.6373967467278874
1114	6	-0.4842698092375741
1114	7	-0.4018235215375938
1114	8	-0.1730152329218626
1114	9	-1.129753156432848
1114	10	-0.3504335214492329
1114	11	-0.3567619108298743
1114	12	-0.39317880686380136
1114	13	-0.33938822811170155
1114	14	-0.689811508914687
1114	15	-1.423544145576571
1114	16	-1.1189975910410368
1114	17	-1.4936302002359128
1114	18	-1.823012542766989
1114	19	-1.6009253960832979
1114	20	-0.8599946174395825
1114	21	-0.6314446091917105
1114	22	-0.47051473574494196
1114	23	-0.32129335927918845
1114	24	-0.8617718360449587
1114	25	-0.6222101264105291
1115	1	-0.6996528786344562
1115	2	-0.5204071328614859
1115	3	-0.5211972803548406
1115	4	-0.6299055561196027
1115	5	-0.35110517072540404
1115	6	-0.39153330524834473
1115	7	-0.39405230611950515
1115	8	-0.2175702013189209
1115	9	-0.5697075653023944
1115	10	-0.25427768267604733
1115	11	-0.5030679931011406
1115	12	-0.5429042239190309
1115	13	-0.46839040405201615
1115	14	-0.46061246218378216
1115	15	-1.6700470986384346
1115	16	-0.9606720289231205
1115	17	-0.6791032082143573
1115	18	-2.3232715832809276
1115	19	-1.2461435081960275
1115	20	-1.0066115483275695
1115	21	-0.9078926456646172
1115	22	-0.781985049702044
1115	23	-0.5127761071809545
1115	24	-0.6063578893036876
1115	25	-0.6688374189190753
1116	1	-1.3849438999545107
1116	2	-0.7661974118181689
1116	3	-1.6419295138291439
1116	4	-0.899807898833302
1116	5	-0.844795082048627
1116	6	-0.4988825907375869
1116	7	-1.161067021858912
1116	8	-2.9881123115636647
1116	9	-1.48512606483704
1116	10	-0.7581584518901039
1116	11	-0.45949826736020327
1116	12	-0.6344573373621856
1116	13	-1.709730386728232
1116	14	-1.5438500788951899
1116	15	-1.5097484180137286
1116	16	-0.9771156705943227
1116	17	-1.626994987078156
1116	18	-2.4323727331048275
1116	19	-1.3443301653516002
1116	20	-0.9408880985319925
1116	21	-0.7207975240130082
1116	22	-0.4783686993017895
1116	23	-0.3975643989973676
1116	24	-0.5073501033423824
1116	25	-0.39805779452584744
1201	1	0.36223441492230535
1201	2	0.3070310827479261
1201	3	0.37168709992150184
1201	4	0.39144473151066705
1201	5	0.2484978436921068
1201	6	0.18527109423720844
1201	7	0.21500480780689912
1201	8	0.2607126732833649
1201	9	0.26391984326712503
1201	10	0.48584620866068207
1201	11	0.28660865768664223
1201	12	0.3848327816729693
1201	13	0.434258444759152
1201	14	0.8018030059424532
1201	15	0.6657604067016862
1201	16	0.5842046834096877
1201	17	0.6203356115318854
1201	18	0.5575738260870793
1201	19	0.5725723035902135
1201	20	0.5601200303379983
1201	21	0.5442721816729801
1201	22	0.5516316234120495
1201	23	0.7517855789304093
1201	24	0.6492508573797267
1201	25	0.778426912809401
1202	1	0.28603727531885426
1202	2	0.2773187803371222
1202	3	0.36476205537713824
1202	4	0.4516351213697618
1202	5	0.23301539603370003
1202	6	0.1105125884061664
1202	7	0.14678479431907476
1202	8	0.3037210926281091
1202	9	0.26203904694715874
1202	10	0.4866931517125437
1202	11	0.23773125755194258
1202	12	0.23978268580892603
1202	13	0.23217204439696104
1202	14	0.6742639715736374
1202	15	0.42842548782380874
1202	16	0.32016577714377803
1202	17	0.4000753405280548
1202	18	0.5193857272732956
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2210	23	0.5650354606208907
2210	24	0.5323375645630009
2210	25	0.6026611359678782
2211	1	0.16065183382855253
2211	2	-0.009959625683194417
2211	3	0.11123482097608317
2211	4	0.09370207123193808
2211	5	0.1397596481110739
2211	6	0.06113966452983277
2211	7	0.1495661933568755
2211	8	0.17862008302354582
2211	9	0.13749559960864133
2211	10	0.24066453655678544
2211	11	0.054555497665852215
2211	12	0.08633991960176957
2211	13	-0.015315732058596154
2211	14	-0.017631968464854708
2211	15	-0.021381365224776516
2211	16	-0.026399084845259324
2211	17	0.06906169557932174
2211	18	0.11571209294055507
2211	19	0.01617443649756467
2211	20	0.05511636656180485
2211	21	0.055263458833451296
2211	22	0.06103350285196285
2211	23	0.1737595141140318
2211	24	0.19778997145943578
2211	25	0.2544205197339977
2212	1	0.15482486635194093
2212	2	0.18345530144103606
2212	3	0.1985244298580362
2212	4	0.09694636110581456
2212	5	0.23041134883784764
2212	6	0.20703091716554667
2212	7	0.232402213620027
2212	8	0.30372702328200774
2212	9	0.1719876796422568
2212	10	0.36952105119453194
2212	11	0.2306715002833144
2212	12	0.18378233938031485
2212	13	0.189631047404049
2212	14	0.2928118857675628
2212	15	0.18892073565456258
2212	16	0.1529238615562889
2212	17	0.16876395826025714
2212	18	0.23884636420003602
2212	19	0.12820930156661348
2212	20	0.202643667800551
2212	21	0.20127163708782092
2212	22	-0.35004168230947386
2212	23	0.27901370447676044
2212	24	0.27292491117347595
2212	25	0.303747390171452
2213	1	-0.09939911506954502
2213	2	-0.9345473159737558
2213	3	-0.2803413784505473
2213	4	-0.14635461807247907
2213	5	0.0603824375241544
2213	6	0.00768255508128135
2213	7	-0.020002472065662857
2213	8	0.14762975125263011
2213	9	-0.2498318727028348
2213	10	-1.5244231833292545
2213	11	-0.40113321149098624
2213	12	-0.1548387064308585
2213	13	-0.13121398838225673
2213	14	-0.0330335456247397
2213	15	-0.02402447612295333
2213	16	-0.09194679666792638
2213	17	-0.19801071953469318
2213	18	0.01841087684552889
2213	19	0.027132782813822587
2213	20	0.007531625767953187
2213	21	-0.02096869034563298
2213	22	0.039136376740600554
2213	23	-0.8272140319940462
2213	24	-1.4135651441472135
2213	25	-1.328155560193899
2214	1	-0.13429799694095124
2214	2	-0.3250692778223687
2214	3	-0.18373038125508145
2214	4	-0.258709470405158
2214	5	-0.19079546782510448
2214	6	-0.26868748155778377
2214	7	-0.23927401923674552
2214	8	-0.1563109859308014
2214	9	-0.45745828484438533
2214	10	-0.1511585363922876
2214	11	-0.2535002803553681
2214	12	-0.3666432777306454
2214	13	-0.2101948242247147
2214	14	-0.25231089564837816
2214	15	-0.30643220708122243
2214	16	-0.4132008025845195
2214	17	-0.32800231540340263
2214	18	-0.29569973331756927
2214	19	-0.8349830362256441
2214	20	-0.5534288779744188
2214	21	-0.47607641172024984
2214	22	-0.7573215030803198
2214	23	-1.9387344530852602
2214	24	-1.3887346748533602
2214	25	-1.7451981484978347
2215	1	-0.4773306703303959
2215	2	-0.31882639337858265
2215	3	-1.2773174245698442
2215	4	-0.9831353453592797
2215	5	-0.3163002381399025
2215	6	-0.4037568113640404
2215	7	-0.31570463150230665
2215	8	-0.230788725752042
2215	9	-0.4709471092451807
2215	10	-1.426523945883261
2215	11	-0.6329309919160124
2215	12	-0.6741886999986733
2215	13	-0.5868332928903257
2215	14	-0.8867238321864903
2215	15	-1.2069422414035813
2215	16	-0.7721998169193824
2215	17	-0.6295372499505731
2215	18	-0.5175147879967668
2215	19	-0.9888195206933403
2215	20	-1.4605313971086673
2215	21	-0.7725999032886577
2215	22	-0.3684535196018359
2215	23	-0.7727278277266336
2215	24	-1.7819039875459595
2215	25	-0.9381612184262096
2216	1	-0.5768142221396602
2216	2	-0.5561116719060131
2216	3	-1.514697649654579
2216	4	-0.9293005952507727
2216	5	-0.5787333668230445
2216	6	-0.5623863146688848
2216	7	-0.47553349046305726
2216	8	-0.5746888566081765
2216	9	-0.4152946886515494
2216	10	-0.47142811182737177
2216	11	-0.5914126625242986
2216	12	-0.4524601579492895
2216	13	-1.2647614266330365
2216	14	-0.9875190932515281
2216	15	-1.3149865871682778
2216	16	-0.9889368009153543
2216	17	-2.047632815052019
2216	18	-0.9932052849846116
2216	19	-0.7043532975949489
2216	20	-0.6617182426253692
2216	21	-0.48434029995096495
2216	22	-0.614586985484415
2216	23	-0.4985617373772655
2216	24	-0.5432811914768934
2216	25	-0.5579243031567813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7570.0
3	744.0
4	1006.0
5	863.0
6	628.0
7	437.0
8	591.0
9	1150.0
10	2392.0
11	4483.0
12	8177.0
13	12498.0
14	16995.0
15	21877.0
16	24888.0
17	26901.0
18	27515.0
19	28509.0
20	29597.0
21	31762.0
22	34565.0
23	36419.0
24	38877.0
25	42124.0
26	44305.0
27	49672.0
28	57075.0
29	66950.0
30	81580.0
31	104437.0
32	139242.0
33	198343.0
34	288176.0
35	465950.0
36	851004.0
37	3026095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.685254895160604	21.504859758620146	12.86194511623753	22.947940229981718
2	14.617720213403556	21.6732564309633	40.80904004839165	22.8999833072415
3	18.09837937653376	27.15471070708333	30.199358553830454	24.547551362552454
4	11.546148156467522	17.81802786124958	39.09056459728151	31.54525938500139
5	12.213012125344596	39.36256382982602	35.44131284944817	12.98311119538121
6	30.47515131909151	39.26601129192037	17.661067449543527	12.597769939444595
7	25.005605466770515	33.54831947064957	24.306397243290448	17.13967781928947
8	23.356161006963564	38.272916260186875	21.756204472816833	16.61471826003273
9	23.66771494528694	16.989022323526314	22.66850874858724	36.67475398259951
10	14.057643431523687	30.981139674735775	34.515801424940626	20.44541546879991
11	31.5425822256664	26.740209329307923	22.85865281898595	18.858555626039724
12	19.805553159327236	28.77286631007318	32.657203522287844	18.76437700831174
13	26.092340135301008	24.661335196090292	27.010120582150897	22.2362040864578
14	19.422773269004555	24.07602742258303	28.913564916701052	27.58763439171136
15	23.092022504047343	31.00653475436877	24.271044783165287	21.630397958418605
16	20.75678007977269	32.33036327797333	26.646806515041515	20.266050127212463
17	19.332742986829327	31.25593152443389	29.477914769330955	19.93341071940583
18	19.565989398373308	30.792761456456613	30.22645616154463	19.41479298362545
19	20.022115906651504	29.726836790093632	30.382503474811262	19.868543828443606
20	21.737814413150414	29.17501335528031	29.214810181461726	19.872362050107554
21	21.522364601509427	30.12135889472915	28.441189201234728	19.915087302526693
22	19.995917847372617	29.68903438692906	29.75227998290772	20.5627677827906
23	19.699136385245392	30.31578165405735	29.520899227125803	20.464182733571455
24	20.15808664229846	29.65717283463692	29.42446782508221	20.7602726979824
25	20.956575927899653	28.936443517113762	28.166228653360783	21.9407519016258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12415.0
1	12415.0
2	10383.0
3	8351.0
4	8351.0
5	8351.0
6	11595.5
7	14840.0
8	14840.0
9	14840.0
10	21732.5
11	28625.0
12	28625.0
13	28625.0
14	46160.5
15	63696.0
16	63696.0
17	63696.0
18	98134.0
19	132572.0
20	132572.0
21	132572.0
22	186474.5
23	240377.0
24	240377.0
25	240377.0
26	308226.0
27	376075.0
28	376075.0
29	376075.0
30	446799.0
31	517523.0
32	517523.0
33	517523.0
34	575758.0
35	633993.0
36	633993.0
37	633993.0
38	671377.0
39	708761.0
40	708761.0
41	708761.0
42	692766.0
43	676771.0
44	676771.0
45	676771.0
46	703913.5
47	731056.0
48	731056.0
49	731056.0
50	660437.0
51	589818.0
52	589818.0
53	589818.0
54	507160.5
55	424503.0
56	424503.0
57	424503.0
58	358355.5
59	292208.0
60	292208.0
61	292208.0
62	234653.0
63	177098.0
64	177098.0
65	177098.0
66	132538.5
67	87979.0
68	87979.0
69	87979.0
70	63019.0
71	38059.0
72	38059.0
73	38059.0
74	25874.0
75	13689.0
76	13689.0
77	13689.0
78	8728.0
79	3767.0
80	3767.0
81	3767.0
82	2352.0
83	937.0
84	937.0
85	937.0
86	579.0
87	221.0
88	221.0
89	221.0
90	130.5
91	40.0
92	40.0
93	40.0
94	23.0
95	6.0
96	6.0
97	6.0
98	11.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3129873105902816
2	0.1804656773126809
3	0.1977518608195487
4	0.21886594668615375
5	0.24488182607224135
6	0.19747472761703377
7	0.19338701287993879
8	0.21742831819810765
9	0.21692601426854932
10	0.22789009659304565
11	0.20199546298305832
12	0.208334884990587
13	0.22101372900564434
14	0.21062123391133503
15	0.20073104274658404
16	0.2039700370509771
17	0.2045416242811641
18	0.20814435591385802
19	0.2066894066006547
20	0.20145851740318568
21	0.19882575197929397
22	0.2031905999189039
23	0.20639495257298257
24	0.22317883215029213
25	0.21715118499559274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5773397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.76567836224736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.90959943322994	57.84244447201486
2	12.12645804985659	16.920211439591604
3	2.896597610565064	6.062492917306096
4	0.9460591740168129	2.640098401844415
5	0.39945619041815256	1.3934166050260732
6	0.1909430322065981	0.7992762102262657
7	0.10714598136252076	0.5232578451481482
8	0.07003211933631515	0.39086706501151086
9	0.054287484209854533	0.34086628462322643
>10	0.2521240020221568	3.240153166793295
>50	0.02545522066148143	1.2237774237315888
>100	0.018591589848052984	2.622099245385143
>500	0.0016750672122470473	0.7867250571482539
>1k	0.0011250321816853469	1.651453170964437
>5k	3.2500929687185015E-4	1.6989998406877038
>10k+	1.2500357571994236E-4	1.8638608544973128
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	34719	0.6013617286322074	No Hit
CCCATGTACTCTGCGTTGATACCAC	32519	0.5632559132864066	No Hit
GAGTACATGGGAAGCAGTGGTATCA	16084	0.27858815182811786	No Hit
CATGTACTCTGCGTTGATACCACTG	13046	0.2259674850005984	No Hit
GCGTTGATACCACTGCTTCCCATGT	10493	0.18174741837431238	No Hit
ATTCCATTCCATTCCATTCCATTCC	9279	0.16071993663349324	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8938	0.15481353525489414	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8670	0.1501715541127693	No Hit
GTATCAACGCAGAGTACATGGGAAG	8646	0.14975585430899693	No Hit
GCTTCCCATGTACTCTGCGTTGATA	7817	0.13539689025369295	No Hit
GGTATCAACGCAGAGTACATGGGAA	7772	0.1346174531216197	No Hit
TATCAACGCAGAGTACATGGGAAGC	7615	0.13189808357194213	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7301	0.12645934447258694	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7065	0.12237162973549194	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6829	0.11828391499839697	No Hit
GAATGGAATGGAATGGAATGGAATG	6626	0.11476778749148898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.732082515718216E-5	0.0
4	0.0	0.0	0.0	5.1962475471546475E-5	0.0
5	0.0	1.732082515718216E-5	0.0	5.1962475471546475E-5	0.0
6	0.0	1.732082515718216E-5	0.0	5.1962475471546475E-5	0.0
7	0.0	1.732082515718216E-5	0.0	5.1962475471546475E-5	0.0
8	0.0	1.732082515718216E-5	0.0	6.928330062872864E-5	0.0
9	0.0	1.732082515718216E-5	0.0	1.212457761002751E-4	0.0
10	1.732082515718216E-5	1.732082515718216E-5	0.0	1.9052907672900373E-4	0.0
11	1.732082515718216E-5	3.464165031436432E-5	0.0	2.078499018861859E-4	0.0
12	1.732082515718216E-5	3.464165031436432E-5	0.0	2.078499018861859E-4	0.0
13	1.732082515718216E-5	3.464165031436432E-5	0.0	2.2517072704336804E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAG	60	4.0597122E-4	12.679674	1
ACCGTTC	60	0.0058822907	11.082482	8
TGCTCGA	60	0.005883051	11.08229	10
CGAGCAC	60	0.005883051	11.08229	10
GTCGTAG	70	0.0014797397	10.868293	1
CTGTCGC	585	0.0	10.717032	9
TAAGACG	260	0.0	10.601783	4
AGACGGT	305	0.0	10.588615	6
GTCTTAC	710	0.0	10.447337	1
TCTGTCG	585	0.0	10.3923645	8
GACGGTG	275	0.0	10.362661	7
AAGACGG	295	0.0	10.310021	5
TATACCG	105	4.087881E-5	9.9571495	5
CCGTTCA	105	4.1133117E-5	9.951531	9
TGTCGCC	610	0.0	9.654829	10
CTAGGAC	640	0.0	9.652641	3
GTCGCCC	595	0.0	9.577517	11
CGTAGGC	80	0.004502376	9.504139	3
TGCGCTA	130	4.2492265E-6	9.499105	10
TCGGAAC	130	4.2572174E-6	9.497704	13
>>END_MODULE