FastQCFastQC Report
Wed 25 May 2016
SRR1295032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5773397
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC364190.630807131399417No Hit
GTACATGGGAAGCAGTGGTATCAAC332720.5762984946297648No Hit
GTATCAACGCAGAGTACTTTTTTTT234080.40544587527931997No Hit
GGTATCAACGCAGAGTACTTTTTTT202490.3507293886077815No Hit
GAGTACATGGGAAGCAGTGGTATCA169660.2938651196167525No Hit
TATCAACGCAGAGTACTTTTTTTTT160920.2787267184293753No Hit
CATGTACTCTGCGTTGATACCACTG145610.2522085351137294No Hit
GCGTTGATACCACTGCTTCCCATGT111050.19234776337050788No Hit
ACGCAGAGTACATGGGAAGCAGTGG108640.18817344450762696No Hit
ACGCAGAGTACTTTTTTTTTTTTTT98900.17130296080453156No Hit
GTATCAACGCAGAGTACATGGGAAG92870.1608585032347507No Hit
ATTCCATTCCATTCCATTCCATTCC87620.15176507002723005No Hit
GCTTCCCATGTACTCTGCGTTGATA85570.14821430087000773No Hit
GGTATCAACGCAGAGTACATGGGAA85490.1480757342687503No Hit
TATCAACGCAGAGTACATGGGAAGC80480.139398000865002No Hit
ACTCTGCGTTGATACCACTGCTTCC79860.13832410970525671No Hit
GAATGGAATGGAATGGAATGGAATG72710.12593971971787146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC500.001501088713.29923
GCGAATT600.00588194811.0825713
CGATTTA600.00588194811.0825714
CCGTTCA951.362896E-510.9992449
GTTCCGA1106.058226E-610.36292311
TATACCG1201.5271016E-610.2910475
CACCGAT750.002650946610.1326367
TAAGACG2350.010.1057754
TAGACAG8750.010.0965365
ACGCCGA856.6029007E-410.0581317
GTCTAAG4900.09.8995061
CTGTCGC5450.09.7607969
AGACGGT2054.1836756E-119.7310376
CTAGGAC6400.09.6478573
GTCTTAC7450.09.5751241
CTAGACA7800.09.499434
CGCCGAG800.0045211519.4993478
GCCGAGT900.00111517969.4993469
GACGGTG2106.730261E-119.4993467
TGTCGCC6000.09.34102310
Produced by FastQC (version 0.11.5)