FastQCFastQC Report
Wed 25 May 2016
SRR1295031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085242
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC376531.220422903616637No Hit
CCCATGTACTCTGCGTTGATACCAC344961.1180970568921336No Hit
GAGTACATGGGAAGCAGTGGTATCA175940.5702632078780205No Hit
CATGTACTCTGCGTTGATACCACTG127820.41429489161628164No Hit
GCGTTGATACCACTGCTTCCCATGT107400.34810883554677396No Hit
GCTTCCCATGTACTCTGCGTTGATA106050.343733165826214No Hit
GTATCAACGCAGAGTACATGGGAAG100140.32457745616065126No Hit
ACGCAGAGTACATGGGAAGCAGTGG89480.2900258715523774No Hit
TATCAACGCAGAGTACATGGGAAGC81340.2636422037558156No Hit
GGTATCAACGCAGAGTACATGGGAA76620.24834356591800577No Hit
ACTCTGCGTTGATACCACTGCTTCC72960.2364806391200431No Hit
GTGGTATCAACGCAGAGTACATGGG49790.1613811817679132No Hit
GTATCAACGCAGAGTACTTTTTTTT45720.14818934786963228No Hit
CAGTGGTATCAACGCAGAGTACATG41350.13402514292233803No Hit
ACATGGGAAGCAGTGGTATCAACGC36420.11804584535021888No Hit
CATGGGAAGCAGTGGTATCAACGCA35630.11548526825448377No Hit
GCAGTGGTATCAACGCAGAGTACAT35480.11499908272997711No Hit
GGTATCAACGCAGAGTACTTTTTTT35380.11467495904697265No Hit
ATACCACTGCTTCCCATGTACTCTG35050.113605350893058No Hit
GTACTCTGCGTTGATACCACTGCTT34420.11156337169012998No Hit
GGGAAGCAGTGGTATCAACGCAGAG34320.11123924800712554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN33380.10819248538688375No Hit
CTGCTTCCCATGTACTCTGCGTTGA32100.10404370224442686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGG600.005860766411.0876755
GTCTTAG2250.010.985471
GTCTCGC2151.8189894E-1210.1699111
GGAACGA1451.6236663E-79.82552815
TTACACC2054.1836756E-119.7358364
GTAGTAC1901.0459189E-99.5034133
GACCGTG800.00452053739.4992457
GAACGAT800.0045253859.49801116
CTAGACT3350.09.362184
CTACTAG1651.05521394E-79.2185761
ACGGTGA1651.067674E-79.2115388
TCGCTCT2401.0913936E-119.1080294
GTGTAGA4300.09.06448651
CGGAGTA950.00182556648.9994319
GTTCTAA3300.08.9304961
GTACTAG2054.092726E-98.81104851
TCTAGAC3350.08.7942033
GAACGAA1201.76868E-48.7065116
TCTACAC3500.08.6888343
ATAGGAC2304.0199666E-108.677033
Produced by FastQC (version 0.11.5)