##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295031_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3085242
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16482985775508	33.0	33.0	33.0	27.0	33.0
2	31.182600911046848	33.0	33.0	33.0	27.0	33.0
3	31.20146523352139	33.0	33.0	33.0	27.0	33.0
4	34.820892494008575	37.0	37.0	37.0	33.0	37.0
5	35.0362999077544	37.0	37.0	37.0	33.0	37.0
6	35.0122188146019	37.0	37.0	37.0	33.0	37.0
7	34.96033050243709	37.0	37.0	37.0	33.0	37.0
8	34.80785656360182	37.0	37.0	37.0	33.0	37.0
9	34.78495917013965	37.0	37.0	37.0	33.0	37.0
10	34.724723700766425	37.0	37.0	37.0	27.0	37.0
11	34.87503443814132	37.0	37.0	37.0	33.0	37.0
12	34.792965025109865	37.0	37.0	37.0	33.0	37.0
13	34.69760589282786	37.0	37.0	37.0	27.0	37.0
14	36.139520335844	40.0	37.0	40.0	27.0	40.0
15	36.268943246591355	40.0	37.0	40.0	27.0	40.0
16	36.35201906365854	40.0	37.0	40.0	27.0	40.0
17	36.28648482031555	40.0	37.0	40.0	27.0	40.0
18	36.22423654287087	40.0	37.0	40.0	27.0	40.0
19	36.319765515962764	40.0	37.0	40.0	27.0	40.0
20	36.31759485965769	40.0	37.0	40.0	27.0	40.0
21	36.32254941427609	40.0	37.0	40.0	27.0	40.0
22	36.23865194367249	40.0	37.0	40.0	27.0	40.0
23	36.15878138570653	40.0	37.0	40.0	27.0	40.0
24	36.21598629864367	40.0	37.0	40.0	27.0	40.0
25	35.0850665847282	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5032811186629402
1101	2	0.4430395185834506
1101	3	0.45650092203068127
1101	4	0.6232786414247187
1101	5	0.5156764635690791
1101	6	0.4403910889646312
1101	7	0.42166378201407895
1101	8	0.480925162532138
1101	9	0.5702648632319267
1101	10	0.6970715555960609
1101	11	0.5709308137204658
1101	12	0.6443424378499998
1101	13	0.5662843461709315
1101	14	1.0554034367718828
1101	15	0.8556052389735598
1101	16	0.8982524008071167
1101	17	0.8056555086143575
1101	18	0.9021579208298078
1101	19	0.7497901700713285
1101	20	0.8049528218412831
1101	21	0.7823641139758806
1101	22	0.845638531893016
1101	23	0.9319633391161659
1101	24	0.8858642485859107
1101	25	1.1397688148923635
1102	1	0.32789966010242466
1102	2	0.33054319655855124
1102	3	0.310924324243409
1102	4	0.47999975742261825
1102	5	0.3065745562400437
1102	6	0.21177105555967302
1102	7	0.19750056060553334
1102	8	0.3225928881013189
1102	9	0.2976685679251929
1102	10	0.5731171030599356
1102	11	0.3994619959436534
1102	12	0.45632464055727695
1102	13	0.4193722267054696
1102	14	1.027693077129932
1102	15	0.8009358653816392
1102	16	0.7444826997848253
1102	17	0.7115078494492266
1102	18	0.765940604077521
1102	19	0.6057272311602659
1102	20	0.7229826543865201
1102	21	0.7467884679929995
1102	22	0.7331378615231685
1102	23	0.749355607662217
1102	24	0.8212857215829317
1102	25	0.9354531308469731
1103	1	0.2905114365439161
1103	2	0.22763396212287645
1103	3	0.25480790986476975
1103	4	0.3095074254411969
1103	5	0.2254868770086631
1103	6	0.20609059039717437
1103	7	0.22408502506301886
1103	8	0.396680535456845
1103	9	0.28297754810403575
1103	10	0.3613316613261901
1103	11	0.1550994286996641
1103	12	0.32046870817909934
1103	13	0.31851026604027766
1103	14	0.6855921019162281
1103	15	0.5782282551146167
1103	16	0.551343884179154
1103	17	0.5125053762577636
1103	18	0.5821727278158448
1103	19	0.602100133682022
1103	20	0.5223911697403736
1103	21	0.5215394144246872
1103	22	0.5560080386591792
1103	23	0.6508333348761397
1103	24	0.6135053874864553
1103	25	0.7160408278913337
1104	1	0.3074654987472414
1104	2	0.3097070969088307
1104	3	0.3212233319582438
1104	4	0.3463889897072505
1104	5	0.13849323551009718
1104	6	0.23406182051738256
1104	7	0.2625128520221338
1104	8	0.34138000907663013
1104	9	0.338971599744994
1104	10	0.4632034121312216
1104	11	0.26867225708077314
1104	12	0.364516022116284
1104	13	0.24665435508962474
1104	14	0.748731562532754
1104	15	0.6193183092911312
1104	16	0.3942660063128258
1104	17	0.5767179934802655
1104	18	0.6923782465054344
1104	19	0.47257298828413496
1104	20	0.44147616019319
1104	21	0.46239625328088607
1104	22	0.6018811149378962
1104	23	0.5386597614338342
1104	24	0.5912759658907731
1104	25	0.5521772015299362
1105	1	0.2151426050472658
1105	2	0.10255065259226726
1105	3	0.09355276460173556
1105	4	0.15672217471549033
1105	5	0.16223584574184713
1105	6	0.13489719936161038
1105	7	0.08310481785754575
1105	8	0.21338074735643175
1105	9	0.210356547238284
1105	10	0.3938832689510363
1105	11	0.23927582126967195
1105	12	0.3045136875759056
1105	13	0.2665550107311603
1105	14	0.5476032880889434
1105	15	0.375119065935003
1105	16	0.36334942412455007
1105	17	0.2940417700677216
1105	18	0.3075274990993577
1105	19	0.3123556752880745
1105	20	0.3110429170816644
1105	21	0.33213837950442837
1105	22	0.2914656819903172
1105	23	0.4244976699830474
1105	24	0.5106765168869103
1105	25	0.5077121997474379
1106	1	-0.08901530748382314
1106	2	-0.10823162501243999
1106	3	-0.24169842877812542
1106	4	-0.03324437844928241
1106	5	-0.5716535261338294
1106	6	-0.38621254328692345
1106	7	-0.6101856906470502
1106	8	-0.17811264779921032
1106	9	0.03187018759021498
1106	10	-0.47930472560731374
1106	11	-0.3468175803257978
1106	12	-0.8360821571277413
1106	13	-0.27573350142802866
1106	14	-0.48304282748978977
1106	15	-0.24148320249295807
1106	16	-0.15194616379111636
1106	17	-0.22148464528481782
1106	18	-0.512215390932603
1106	19	-0.6923938960611551
1106	20	-0.20905838939760457
1106	21	-0.05108930046852578
1106	22	0.14355218492446653
1106	23	-0.5641968167227418
1106	24	0.07420960550693678
1106	25	0.09749018681949195
1107	1	-1.0296277786486776
1107	2	-1.0685636931945695
1107	3	-0.7434027876224292
1107	4	-0.8673190690119554
1107	5	-0.9888592424406752
1107	6	-0.937514133179711
1107	7	-0.9539543836933788
1107	8	-0.9306100096382792
1107	9	-1.0250428143162509
1107	10	-0.8257647800612915
1107	11	-0.8243924836904029
1107	12	-0.9361472609148436
1107	13	-0.8115019593233725
1107	14	-1.1369400665061988
1107	15	-0.9865399788254976
1107	16	-1.0481525078150753
1107	17	-1.0860178511313308
1107	18	-1.155785663183039
1107	19	-1.196144214292353
1107	20	-1.3209088819514818
1107	21	-1.2191503056321267
1107	22	-1.174571834874861
1107	23	-0.8492489872052644
1107	24	-0.6882659805729006
1107	25	-0.8413912523823157
1108	1	0.04272387898338792
1108	2	0.007888677031413494
1108	3	0.007559349042079333
1108	4	-0.010365081436312096
1108	5	-0.3964191653040672
1108	6	0.028794186830587876
1108	7	0.003950897326568281
1108	8	0.16808364252183594
1108	9	-0.17167696619058148
1108	10	-0.4643111216055189
1108	11	-0.11142885637516997
1108	12	0.06868620272830128
1108	13	0.16594477986224376
1108	14	-0.2802646254335244
1108	15	0.05680562933047639
1108	16	-0.6747328620241078
1108	17	-0.7130333360144405
1108	18	-0.6194385730849064
1108	19	0.03680502902929561
1108	20	-0.9345820020865006
1108	21	-0.24376277998700857
1108	22	-0.30979569055404
1108	23	-0.11651990054850359
1108	24	-0.3144911954247789
1108	25	-1.1302603310654433
1109	1	0.0881240360590283
1109	2	0.0632980475985434
1109	3	0.09256350533192403
1109	4	0.17206171635195489
1109	5	0.10073945898693637
1109	6	0.09879411592549303
1109	7	0.16261247606605167
1109	8	0.17851469962086952
1109	9	0.13562100329603766
1109	10	0.28481213689234863
1109	11	0.10757587799974289
1109	12	0.22903578153118076
1109	13	0.20317787952853195
1109	14	0.4548058271203317
1109	15	0.345080262281904
1109	16	0.22786697756354357
1109	17	0.30743312775243226
1109	18	0.34568216786500017
1109	19	0.2154274347891274
1109	20	0.23442750795511813
1109	21	0.27823460820295765
1109	22	0.37541546690490435
1109	23	0.44142516134956367
1109	24	0.4523985859414452
1109	25	0.2974854166100229
1110	1	-0.05855585894932247
1110	2	-0.09402598010771612
1110	3	6.271262894586016E-5
1110	4	-0.9732459317150699
1110	5	-0.2665158656141031
1110	6	-0.04862500833844763
1110	7	-0.0695161395221433
1110	8	0.09640031916301695
1110	9	0.08859536060157325
1110	10	-0.07448557814437606
1110	11	-0.02720554120595864
1110	12	0.10171460085098971
1110	13	0.058442743710998
1110	14	0.12209371018719395
1110	15	0.09084287852388684
1110	16	0.0717273846539328
1110	17	0.15242048947502695
1110	18	0.1261212672628389
1110	19	0.02207814328325952
1110	20	0.05434133513163886
1110	21	0.16162885851852593
1110	22	-0.44487373926867946
1110	23	-0.10452708220019247
1110	24	0.09789625378393652
1110	25	0.20239008538936076
1111	1	-0.15448926056691192
1111	2	-0.3483261840249732
1111	3	-0.24433245065901588
1111	4	-0.12311877120045267
1111	5	-0.22455090274295486
1111	6	-0.19714729358165073
1111	7	-0.07218942668673378
1111	8	-0.06818442608195596
1111	9	-0.13862985892043866
1111	10	-0.11948343094047686
1111	11	-0.19117797336932085
1111	12	-0.0435094343939042
1111	13	-0.27495871931621707
1111	14	-0.36994556896078024
1111	15	-0.18605873105335036
1111	16	-0.3065188732362003
1111	17	-0.15511792489359522
1111	18	-0.22052741479161853
1111	19	-0.23383000275849497
1111	20	-0.17368822152261032
1111	21	-0.20078019444131456
1111	22	-0.20385764609947188
1111	23	0.013740616002920092
1111	24	-0.05629279501246032
1111	25	-0.16196438555773796
1112	1	-0.12069063357302667
1112	2	-0.3652660293236316
1112	3	-0.09410564187687953
1112	4	-0.29139726543443345
1112	5	-0.0916747648692322
1112	6	-0.17364198995358038
1112	7	-0.0678539721959055
1112	8	0.015755242198594033
1112	9	-0.22729602076525168
1112	10	0.09559250631817662
1112	11	-0.13991142494047182
1112	12	-0.08817609184045239
1112	13	-0.18145505531885675
1112	14	-0.3388100873644433
1112	15	-0.28881874072549607
1112	16	-0.2597594937958334
1112	17	-0.3591765963390543
1112	18	-0.2274886038474051
1112	19	-0.13150362420909545
1112	20	-0.9195909908095175
1112	21	-1.3681231322817666
1112	22	-1.7637826400319412
1112	23	-0.6803243655117654
1112	24	-0.41645299986553397
1112	25	-1.5370161416291523
1113	1	-0.19775724964863173
1113	2	-0.1721785681203336
1113	3	-0.2762615951791254
1113	4	-0.5022720570900532
1113	5	-0.23646746087229786
1113	6	-0.2680871757608827
1113	7	-0.3222078143281166
1113	8	-0.0670093854623488
1113	9	-0.21328577289634154
1113	10	-0.15981900318517717
1113	11	-0.19624978725729392
1113	12	-0.2952258353099211
1113	13	-0.21088757574318606
1113	14	-0.6795762478582219
1113	15	-0.5305776183160873
1113	16	-0.5199044572128173
1113	17	-0.508844338171734
1113	18	-1.037413716308265
1113	19	-1.0689914092736004
1113	20	-1.1471156883261884
1113	21	-1.125561357393913
1113	22	-0.5106850436610202
1113	23	-1.9668977402070524
1113	24	-1.1399787490489075
1113	25	-0.5874764723271468
1114	1	-0.1668920137546479
1114	2	-0.34693965001318006
1114	3	-0.27365691812038406
1114	4	-0.42872847290961147
1114	5	-0.5835458283833077
1114	6	-0.3281223136221385
1114	7	-0.40449934965822365
1114	8	-0.16425315901710746
1114	9	-1.062729446832968
1114	10	-0.29334552869703145
1114	11	-0.2548779594643307
1114	12	-0.3200870658809478
1114	13	-0.325834477282271
1114	14	-0.7325261554180926
1114	15	-1.0721021034039495
1114	16	-1.0946644780102872
1114	17	-1.4733961845400998
1114	18	-1.655218943513752
1114	19	-1.606400794849442
1114	20	-0.8709569565723427
1114	21	-0.5340133253513315
1114	22	-0.513721024781006
1114	23	-0.2586571486900766
1114	24	-0.8556302335213317
1114	25	-0.5125904464538991
1115	1	-0.7972343563128916
1115	2	-0.7255329287899954
1115	3	-0.6891960652375602
1115	4	-0.7391181971461052
1115	5	-0.5838192206037078
1115	6	-0.5343816868444549
1115	7	-0.5339464667104536
1115	8	-0.32626721774693124
1115	9	-0.7606704597022684
1115	10	-0.3624177420829682
1115	11	-0.6327326535488424
1115	12	-0.6458300336780596
1115	13	-0.5673388219166569
1115	14	-0.6400451667410039
1115	15	-1.6798530881835418
1115	16	-0.994942142969542
1115	17	-0.8257091237177434
1115	18	-2.3994419956178348
1115	19	-1.3691324529748314
1115	20	-1.1332013490361774
1115	21	-0.9577089890416914
1115	22	-0.8266173173680755
1115	23	-0.6624218257845342
1115	24	-0.6289522138774544
1115	25	-0.8278774231458712
1116	1	-1.566965629968994
1116	2	-0.9133287264861494
1116	3	-1.731136875604136
1116	4	-1.137946639311032
1116	5	-1.116817606737328
1116	6	-0.48160820814213423
1116	7	-1.2265766104837752
1116	8	-3.1251893005474045
1116	9	-1.4883955652904177
1116	10	-0.8487343399176766
1116	11	-0.6902083203276348
1116	12	-0.6636669558344295
1116	13	-1.7132851337106416
1116	14	-1.7246918853235655
1116	15	-1.540769014456849
1116	16	-1.0513081714228605
1116	17	-1.7719626127009818
1116	18	-2.7380214165253562
1116	19	-1.472856287153327
1116	20	-0.9991903713466783
1116	21	-0.718616692506707
1116	22	-0.5555069601479659
1116	23	-0.44259714791866145
1116	24	-0.5333554956595492
1116	25	-0.45956645929729234
1201	1	0.3528191358037738
1201	2	0.3788757581856643
1201	3	0.38802349738783803
1201	4	0.41034093811870775
1201	5	0.3435990012967238
1201	6	0.2322864206873021
1201	7	0.23944507757273215
1201	8	0.3918869620226033
1201	9	0.3780164078930355
1201	10	0.5941545839415738
1201	11	0.4147042275545729
1201	12	0.4956304992258964
1201	13	0.48078427118844047
1201	14	0.8544291156181245
1201	15	0.7265933422532882
1201	16	0.7357043371771397
1201	17	0.7623061206349675
1201	18	0.6255245943227692
1201	19	0.5738273000608913
1201	20	0.6924485711759161
1201	21	0.569343253124508
1201	22	0.56938824437119
1201	23	0.6897256035320254
1201	24	0.7145284118902566
1201	25	0.742226066989609
1202	1	0.3511172121506334
1202	2	0.4115758640565019
1202	3	0.418062849378412
1202	4	0.5560291984411165
1202	5	0.34926209232566663
1202	6	0.09819800855011351
1202	7	0.19131979042305858
1202	8	0.3206958152217041
1202	9	0.3019372244967826
1202	10	0.4445431099398576
1202	11	0.39679008281115813
1202	12	0.4009839679940015
1202	13	0.3308691676148001
1202	14	0.7214793143756353
1202	15	0.5497750605569607
1202	16	0.3676473666811475
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2210	24	0.3631893331480214
2210	25	0.43144796137649166
2211	1	0.20308403484916226
2211	2	0.023041795909495733
2211	3	0.10482022518668899
2211	4	0.13553073555134887
2211	5	0.11754049315974413
2211	6	0.046826570593310635
2211	7	0.11043788904805041
2211	8	0.2011396134014518
2211	9	0.1284181882523967
2211	10	0.26831217207053015
2211	11	0.03282898009077684
2211	12	0.18051076655579124
2211	13	0.06227084790301518
2211	14	-0.011921858898283233
2211	15	0.09483220678059467
2211	16	-0.06367656546463252
2211	17	0.20389272314415763
2211	18	0.22843994339400098
2211	19	-0.025382593820268085
2211	20	0.040258735529029366
2211	21	0.12977815780649138
2211	22	0.03742587372907735
2211	23	0.17947370263855333
2211	24	0.20533629294203593
2211	25	0.23374953637627982
2212	1	0.009573812511515456
2212	2	6.446952129941508E-4
2212	3	0.04695263850649667
2212	4	-0.060824574976741985
2212	5	0.10616641350260636
2212	6	0.01648779785094945
2212	7	0.03665669054631593
2212	8	0.10015901736868216
2212	9	0.026463771139873415
2212	10	0.27073413935378454
2212	11	0.07781670676156693
2212	12	0.0632807123624417
2212	13	-0.036958375731281023
2212	14	-0.02882693490635546
2212	15	0.06788389026232267
2212	16	-0.1162190704533188
2212	17	-0.05561918540401223
2212	18	0.1366042792886475
2212	19	0.043967597227130284
2212	20	0.017713197494252597
2212	21	0.10421972603246843
2212	22	-0.4346330125313784
2212	23	0.09934584735657381
2212	24	0.05578861515127187
2212	25	0.13617147229400928
2213	1	-0.00972819598632313
2213	2	-0.7435518452544194
2213	3	-0.0965850476291017
2213	4	-0.12150779944472134
2213	5	-0.044740898444196375
2213	6	-0.09143785662460857
2213	7	-0.0445006946316866
2213	8	0.14658488172336348
2213	9	-0.27028514096154765
2213	10	-1.620737747414168
2213	11	-0.4095829211937243
2213	12	-0.1658782159555372
2213	13	-0.15741139753757238
2213	14	-0.03760343025730606
2213	15	-0.20483356111065376
2213	16	-0.1436273200614977
2213	17	-0.22757752364459094
2213	18	-0.051494895388152884
2213	19	-0.0833161593372509
2213	20	-0.13249713459792645
2213	21	-0.06695722773071111
2213	22	0.123636224625983
2213	23	-0.9324790742029947
2213	24	-1.4069376088667482
2213	25	-1.349889006109585
2214	1	-0.054184216060708223
2214	2	-0.28237762828977253
2214	3	-0.06525019079350614
2214	4	-0.10154655913463984
2214	5	-0.031006973633516566
2214	6	-0.1923620399825836
2214	7	-0.021052202450839275
2214	8	-0.048490621256434
2214	9	-0.3075851812895465
2214	10	-0.03331000174942744
2214	11	-0.11463176288391708
2214	12	-0.17378586483307146
2214	13	-0.07121141546112852
2214	14	-0.01997897356509526
2214	15	-0.2958661819826318
2214	16	-0.36455083128612387
2214	17	-0.2827059011146815
2214	18	-0.08722214367421799
2214	19	-0.7333105582259876
2214	20	-0.41688571729272894
2214	21	-0.3224239123209358
2214	22	-0.5852964276122279
2214	23	-1.8689669533982283
2214	24	-1.2154087295370886
2214	25	-1.6852308979409258
2215	1	-0.5472709980978934
2215	2	-0.26314748419482825
2215	3	-1.295069436747852
2215	4	-1.0998338801980339
2215	5	-0.5446410268505346
2215	6	-0.5344759742828558
2215	7	-0.4007055146250309
2215	8	-0.27712897427131367
2215	9	-0.4411241217957098
2215	10	-1.4792627985389117
2215	11	-0.6851797728606286
2215	12	-0.6284055617490409
2215	13	-0.6177311649794319
2215	14	-1.0072329542027063
2215	15	-1.2802919003887183
2215	16	-0.8674208355415942
2215	17	-0.7164483801624044
2215	18	-0.5791263335391079
2215	19	-1.1316186394818288
2215	20	-1.3905793027626672
2215	21	-0.8328112487513053
2215	22	-0.5627530011313766
2215	23	-0.7838520113790111
2215	24	-1.9758796238950467
2215	25	-1.085099687614317
2216	1	-0.6002001786964257
2216	2	-0.6169789447688885
2216	3	-1.4233153507276235
2216	4	-0.9624694374824969
2216	5	-0.6067841682161443
2216	6	-0.6514829199233461
2216	7	-0.5131454937791986
2216	8	-0.5813416061909678
2216	9	-0.39160128195226207
2216	10	-0.4167767922673491
2216	11	-0.4714158830643953
2216	12	-0.488854682482625
2216	13	-1.1443950081546532
2216	14	-0.8230028839445396
2216	15	-1.291069743825254
2216	16	-1.0255438422704586
2216	17	-2.157558687861105
2216	18	-1.1147870676567777
2216	19	-0.7104691419197806
2216	20	-0.8211701498991886
2216	21	-0.5394280923006818
2216	22	-0.6614351037094934
2216	23	-0.596802149163409
2216	24	-0.6015476481142201
2216	25	-0.5432553958677175
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3721.0
3	456.0
4	545.0
5	393.0
6	271.0
7	232.0
8	272.0
9	649.0
10	1257.0
11	2407.0
12	4226.0
13	6455.0
14	9183.0
15	12508.0
16	14825.0
17	16021.0
18	16347.0
19	16479.0
20	17146.0
21	18061.0
22	19188.0
23	20206.0
24	21279.0
25	22592.0
26	23888.0
27	27075.0
28	29778.0
29	35399.0
30	42971.0
31	55249.0
32	73910.0
33	105323.0
34	153016.0
35	246391.0
36	452133.0
37	1615390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24084330519889	20.59957304294013	13.027033246485795	23.132550405375188
2	14.341374948703697	21.43464721129921	40.18643284210089	24.0375449978962
3	18.791624859153327	27.058601956753986	29.314811387230115	24.83496179686257
4	11.767638498831262	18.2287466453781	38.12104148120096	31.882573374589672
5	12.109998096244274	39.38691918394503	35.3625225218201	13.140560197990595
6	30.734585460260554	37.85287508605125	17.982244216706285	13.430295236981907
7	25.162527377432713	33.67197736095349	23.357280976479355	17.80821428513444
8	24.08306073825416	37.63416484575752	21.30551391209231	16.977260503896012
9	24.269746196652285	17.43294409415011	21.662079676570105	36.6352300326275
10	14.355853567591234	31.18493347347579	34.013009082842125	20.446203876090856
11	30.961884117976567	26.75674841894185	22.553820924360878	19.727546538720702
12	20.82360288264906	29.041348042105042	31.36649139677149	18.76855767847441
13	26.665830865989104	24.035332282736213	25.950474262752348	23.348362588522335
14	19.862588399124974	24.78923206426571	28.056622205132737	27.291557331476586
15	24.455207462310945	29.896654462205408	23.58496172237151	22.063176353112137
16	20.418258704819728	31.540216133995543	27.46174074961697	20.57978441156776
17	20.825373134813045	30.783829098231692	28.532144263964565	19.8586535029907
18	20.973279467927565	29.76712088540178	29.450585851237953	19.809013795432698
19	20.824919772134027	29.984373345838122	29.797223703413117	19.393483178614733
20	21.990017600717017	29.108143741353892	28.65779919595248	20.244039461976605
21	21.26187017793049	29.78177025135243	28.731229263822467	20.225130306894616
22	20.654418510249645	29.454418510249646	27.943105337933833	21.948057641566876
23	19.718283330042503	30.237095617654084	28.59218956218109	21.452431490122315
24	20.572932483702395	30.549524218151912	28.13422124724773	20.743322050897955
25	21.254121489426385	28.63717382097471	26.849766026715304	23.2589386628836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7618.0
1	7618.0
2	7049.5
3	6481.0
4	6481.0
5	6481.0
6	8593.0
7	10705.0
8	10705.0
9	10705.0
10	13865.0
11	17025.0
12	17025.0
13	17025.0
14	25153.5
15	33282.0
16	33282.0
17	33282.0
18	50217.5
19	67153.0
20	67153.0
21	67153.0
22	92981.0
23	118809.0
24	118809.0
25	118809.0
26	151529.0
27	184249.0
28	184249.0
29	184249.0
30	217141.5
31	250034.0
32	250034.0
33	250034.0
34	275934.5
35	301835.0
36	301835.0
37	301835.0
38	317796.0
39	333757.0
40	333757.0
41	333757.0
42	336024.5
43	338292.0
44	338292.0
45	338292.0
46	396037.0
47	453782.0
48	453782.0
49	453782.0
50	406529.0
51	359276.0
52	359276.0
53	359276.0
54	299416.0
55	239556.0
56	239556.0
57	239556.0
58	201765.0
59	163974.0
60	163974.0
61	163974.0
62	134570.5
63	105167.0
64	105167.0
65	105167.0
66	80561.5
67	55956.0
68	55956.0
69	55956.0
70	40339.0
71	24722.0
72	24722.0
73	24722.0
74	17086.0
75	9450.0
76	9450.0
77	9450.0
78	6220.5
79	2991.0
80	2991.0
81	2991.0
82	1906.0
83	821.0
84	821.0
85	821.0
86	516.0
87	211.0
88	211.0
89	211.0
90	144.0
91	77.0
92	77.0
93	77.0
94	41.5
95	6.0
96	6.0
97	6.0
98	9.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2782925942276165
2	0.16523825359566607
3	0.18319470563411233
4	0.20358208529509195
5	0.2306464128259631
6	0.18416707668312565
7	0.18173614906059232
8	0.20076220925295324
9	0.20238282766797547
10	0.2097080229038759
11	0.18854274640368568
12	0.194182498487963
13	0.20607783765422613
14	0.19531693137847858
15	0.18692212798866345
16	0.189806828767403
17	0.18990406587230435
18	0.19324253980725015
19	0.19084402455301724
20	0.1887048082451879
21	0.18737590114486968
22	0.1901633648187079
23	0.19376113770005723
24	0.2079253426473515
25	0.2028366008241817
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3085242.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.1059238911146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71288837256812	65.74150553898478
2	8.753207604491353	12.973290730831236
3	1.4597802471644965	3.245350916823739
4	0.3896509876083534	1.155017857272091
5	0.18323465985602513	0.6789386878752435
6	0.10519592071963885	0.46773845367031686
7	0.06572954396409046	0.34096640076803914
8	0.04953236681263073	0.29365134441308666
9	0.03634965595395531	0.24243523538328118
>10	0.2125557223606412	2.9020463770064606
>50	0.01705521304658733	0.8749394931320758
>100	0.01129569864313002	1.5942297333567061
>500	0.0012334023840227424	0.6314275256568593
>1k	0.0018060534905447203	3.4420846820689923
>5k	1.7620034054094833E-4	1.0459048709598509
>10k+	3.083505959466596E-4	4.370472151797399
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	37653	1.220422903616637	No Hit
CCCATGTACTCTGCGTTGATACCAC	34496	1.1180970568921336	No Hit
GAGTACATGGGAAGCAGTGGTATCA	17594	0.5702632078780205	No Hit
CATGTACTCTGCGTTGATACCACTG	12782	0.41429489161628164	No Hit
GCGTTGATACCACTGCTTCCCATGT	10740	0.34810883554677396	No Hit
GCTTCCCATGTACTCTGCGTTGATA	10605	0.343733165826214	No Hit
GTATCAACGCAGAGTACATGGGAAG	10014	0.32457745616065126	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8948	0.2900258715523774	No Hit
TATCAACGCAGAGTACATGGGAAGC	8134	0.2636422037558156	No Hit
GGTATCAACGCAGAGTACATGGGAA	7662	0.24834356591800577	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7296	0.2364806391200431	No Hit
GTGGTATCAACGCAGAGTACATGGG	4979	0.1613811817679132	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4572	0.14818934786963228	No Hit
CAGTGGTATCAACGCAGAGTACATG	4135	0.13402514292233803	No Hit
ACATGGGAAGCAGTGGTATCAACGC	3642	0.11804584535021888	No Hit
CATGGGAAGCAGTGGTATCAACGCA	3563	0.11548526825448377	No Hit
GCAGTGGTATCAACGCAGAGTACAT	3548	0.11499908272997711	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3538	0.11467495904697265	No Hit
ATACCACTGCTTCCCATGTACTCTG	3505	0.113605350893058	No Hit
GTACTCTGCGTTGATACCACTGCTT	3442	0.11156337169012998	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	3432	0.11123924800712554	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3338	0.10819248538688375	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	3210	0.10404370224442686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.2412368300444505E-5	0.0	0.0	0.0	0.0
6	3.2412368300444505E-5	0.0	0.0	0.0	0.0
7	3.2412368300444505E-5	0.0	0.0	3.2412368300444505E-5	0.0
8	3.2412368300444505E-5	0.0	0.0	3.2412368300444505E-5	0.0
9	3.2412368300444505E-5	0.0	0.0	3.2412368300444505E-5	0.0
10	3.2412368300444505E-5	0.0	0.0	3.2412368300444505E-5	0.0
11	3.2412368300444505E-5	3.2412368300444505E-5	0.0	6.482473660088901E-5	0.0
12	3.2412368300444505E-5	3.2412368300444505E-5	0.0	6.482473660088901E-5	0.0
13	3.2412368300444505E-5	3.2412368300444505E-5	0.0	6.482473660088901E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACGG	60	0.0058607664	11.087675	5
GTCTTAG	225	0.0	10.98547	1
GTCTCGC	215	1.8189894E-12	10.169911	1
GGAACGA	145	1.6236663E-7	9.825528	15
TTACACC	205	4.1836756E-11	9.735836	4
GTAGTAC	190	1.0459189E-9	9.503413	3
GACCGTG	80	0.0045205373	9.499245	7
GAACGAT	80	0.004525385	9.498011	16
CTAGACT	335	0.0	9.36218	4
CTACTAG	165	1.05521394E-7	9.218576	1
ACGGTGA	165	1.067674E-7	9.211538	8
TCGCTCT	240	1.0913936E-11	9.108029	4
GTGTAGA	430	0.0	9.0644865	1
CGGAGTA	95	0.0018255664	8.999431	9
GTTCTAA	330	0.0	8.930496	1
GTACTAG	205	4.092726E-9	8.8110485	1
TCTAGAC	335	0.0	8.794203	3
GAACGAA	120	1.76868E-4	8.70651	16
TCTACAC	350	0.0	8.688834	3
ATAGGAC	230	4.0199666E-10	8.67703	3
>>END_MODULE