FastQCFastQC Report
Wed 25 May 2016
SRR1295031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085242
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC376031.2188022852016147No Hit
CCCATGTACTCTGCGTTGATACCAC372461.207231069718356No Hit
GAGTACATGGGAAGCAGTGGTATCA187450.6075698437918322No Hit
CATGTACTCTGCGTTGATACCACTG143840.46621950563359377No Hit
GTATCAACGCAGAGTACTTTTTTTT125260.4059973253313679No Hit
GCTTCCCATGTACTCTGCGTTGATA115560.3745573280799367No Hit
GCGTTGATACCACTGCTTCCCATGT111000.359777288134934No Hit
ACGCAGAGTACATGGGAAGCAGTGG109580.35517473183627085No Hit
GTATCAACGCAGAGTACATGGGAAG107060.3470068150245588No Hit
GGTATCAACGCAGAGTACTTTTTTT105530.3420477226745908No Hit
TATCAACGCAGAGTACATGGGAAGC91940.29799931415428677No Hit
TATCAACGCAGAGTACTTTTTTTTT85790.2780657076495134No Hit
GGTATCAACGCAGAGTACATGGGAA83580.27090257425511516No Hit
ACTCTGCGTTGATACCACTGCTTCC81750.26497111085613384No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53000.17178555199235587No Hit
GTGGTATCAACGCAGAGTACATGGG52390.16980839752602875No Hit
CAGTGGTATCAACGCAGAGTACATG50210.16274250123653183No Hit
ACATGGGAAGCAGTGGTATCAACGC46270.1499720281261567No Hit
CATGGGAAGCAGTGGTATCAACGCA43580.14125310105333716No Hit
GCAGTGGTATCAACGCAGAGTACAT42760.1385952868527007No Hit
GGGAAGCAGTGGTATCAACGCAGAG41280.13379825634423492No Hit
ATACCACTGCTTCCCATGTACTCTG37160.12044436060445177No Hit
CTGCTTCCCATGTACTCTGCGTTGA35290.11438324773226866No Hit
CCATGTACTCTGCGTTGATACCACT34370.11140130984862776No Hit
GTACTCTGCGTTGATACCACTGCTT34090.11049376353621532No Hit
GATACCACTGCTTCCCATGTACTCT32000.10371957856142242No Hit
CTTCCCATGTACTCTGCGTTGATAC31680.10268238277580817No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31240.10125623857058863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA3700.012.3234214
GGACGCA600.00588109211.0825218
TAGACAG4750.010.9991925
GCACCGC803.775502E-410.6867166
GTCTCGC1553.590685E-910.4327931
GGTACAC1606.188202E-910.093013
ACGATTC951.6462334E-49.99926618
CTAGGAC3050.09.9664823
TATTCCG1054.1110867E-59.9516515
TTACACT5050.09.7814614
ATTAGAC2950.09.6603093
TATACTG7050.09.5666745
GTCCTAG3100.09.5122521
GTCTAGG2700.09.5122521
TCTCGCT2106.730261E-119.4996112
GGGTAAC1752.61698E-89.2404741
TAGACTG4250.09.1640345
TCTGTCG2401.0913936E-119.1034998
GTAACAC2401.0913936E-119.1034993
TGGACAG4700.09.09507755
Produced by FastQC (version 0.11.5)