FastQCFastQC Report
Wed 25 May 2016
SRR1295030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382656
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2585147.642337855223824No Hit
CCCATGTACTCTGCGTTGATACCAC2471827.307334828016801No Hit
GAGTACATGGGAAGCAGTGGTATCA1177463.4808742006281452No Hit
CATGTACTCTGCGTTGATACCACTG928522.7449436182691946No Hit
GCGTTGATACCACTGCTTCCCATGT793962.3471496953872935No Hit
GTATCAACGCAGAGTACATGGGAAG659381.9492966473682218No Hit
ACGCAGAGTACATGGGAAGCAGTGG649561.9202662050175956No Hit
TATCAACGCAGAGTACATGGGAAGC644751.906046609528134No Hit
ACTCTGCGTTGATACCACTGCTTCC575851.7023605119763876No Hit
GGTATCAACGCAGAGTACATGGGAA554091.6380323627350817No Hit
GCTTCCCATGTACTCTGCGTTGATA436221.289578366821811No Hit
GTGGTATCAACGCAGAGTACATGGG301690.8918731316456654No Hit
ATACCACTGCTTCCCATGTACTCTG294230.8698194554811366No Hit
GTACTCTGCGTTGATACCACTGCTT293580.8678978885231013No Hit
CAGTGGTATCAACGCAGAGTACATG277340.8198882771408029No Hit
GGGAAGCAGTGGTATCAACGCAGAG260920.7713465395239717No Hit
ACATGGGAAGCAGTGGTATCAACGC247870.7327673875203391No Hit
CATGGGAAGCAGTGGTATCAACGCA243300.7192572936769213No Hit
GCAGTGGTATCAACGCAGAGTACAT243050.7185182294622924No Hit
CTGCTTCCCATGTACTCTGCGTTGA238760.7058358875392591No Hit
TACCACTGCTTCCCATGTACTCTGC236520.6992138721761835No Hit
GATACCACTGCTTCCCATGTACTCT231220.6835457108260491No Hit
GTTGATACCACTGCTTCCCATGTAC230010.6799686400272449No Hit
CCATGTACTCTGCGTTGATACCACT216430.6398226718885988No Hit
GCAGAGTACATGGGAAGCAGTGGTA215760.6378419797933932No Hit
CTTCCCATGTACTCTGCGTTGATAC202000.5971638854202141No Hit
CCACTGCTTCCCATGTACTCTGCGT195050.5766179002535285No Hit
AAGCAGTGGTATCAACGCAGAGTAC182490.5394873141105687No Hit
GTACATGGTAAGCAGTGGTATCAAC172080.5087126802134181No Hit
TTCCCATGTACTCTGCGTTGATACC166800.49310364400045414No Hit
ATGGGAAGCAGTGGTATCAACGCAG166430.4920098289628032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA162910.48160380482082715No Hit
TGATACCACTGCTTCCCATGTACTC144680.42771124229008206No Hit
ACCATGTACTCTGCGTTGATACCAC133570.3948672285919703No Hit
ATCAACGCAGAGTACATGGGAAGCA125570.371217173723843No Hit
CCCCATGTACTCTGCGTTGATACCA91300.2699062511825028No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA89860.26564924130623985No Hit
GTATCAACGCAGAGTACTTTTTTTT87970.2600619158436448No Hit
TCCATGTACTCTGCGTTGATACCAC81560.24111230938055778No Hit
GGAAGCAGTGGTATCAACGCAGAGT77670.2296124702009309No Hit
GGTAAGCAGTGGTATCAACGCAGAG75260.22248789117190754No Hit
GTACATGGGTAAGCAGTGGTATCAA72660.21480162333976616No Hit
AAAAAGTACTCTGCGTTGATACCAC72030.21293918151890112No Hit
GGTATCAACGCAGAGTACTTTTTTT64850.19171325727475688No Hit
GAGTACATGGTAAGCAGTGGTATCA62350.1843226151284671No Hit
TATCAACGCAGAGTACTTTTTTTTT62210.18390873916827485No Hit
CTGCGTTGATACCACTGCTTCCCAT60410.17858747682294623No Hit
GAACAAAAAAAAAAAAAAAAAAAAA59950.17722759866802892No Hit
CTCTGCGTTGATACCACTGCTTCCC59350.17545384455291935No Hit
GTACATGGAAAGCAGTGGTATCAAC59270.17521734400423808No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58210.17208371173421122No Hit
TCCCATGTACTCTGCGTTGATACCA58100.1717585234797745No Hit
TGGGAAGCAGTGGTATCAACGCAGA55160.1630671283157377No Hit
TCAACGCAGAGTACATGGGAAGCAG54910.1623280641011087No Hit
GCTTACCATGTACTCTGCGTTGATA54510.16114556135770236No Hit
AAAGTACTCTGCGTTGATACCACTG51200.15136035115601468No Hit
AGTGGTATCAACGCAGAGTACATGG44970.13294287092746054No Hit
ACTCTGCGTTGATACCACTGCTTAC44560.13173080561546904No Hit
GCGTTGATACCACTGCTTACCATGT43120.1274737957392061No Hit
TTGATACCACTGCTTCCCATGTACT41750.1234237238430393No Hit
GAGTACATGGAAAGCAGTGGTATCA40470.11963971506413894No Hit
AACGCAGAGTACATGGGAAGCAGTG40210.11887108828092481No Hit
GTACATGGAAGCAGTGGTATCAACG38730.11449582813032126No Hit
ACTGCTTCCCATGTACTCTGCGTTG38200.11292901199530783No Hit
ACGCAGAGTACATGGTAAGCAGTGG38190.11289944942672267No Hit
CTGCTTACCATGTACTCTGCGTTGA36680.10843550157036365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN36040.10654349718091345No Hit
GTATCAACGCAGAGTACATGGTAAG35780.10577487039769932No Hit
TATCAACGCAGAGTACATGGTAAGC34380.10163611079577704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34310.10142917281568092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA13250.017.0826511
GACTAGA453.5081168E-516.90721
TATGCGT456.773516E-414.77539310
CGCCGGC400.005293732514.24474719
TACATAA9200.013.6391452
GTACATA11800.013.4594911
AGAACAA16450.013.2911622
GTCTTGG1051.9665094E-812.68041
TACAAAA18800.012.388232
GTACAAG4350.012.2431441
TATTAGG1103.785499E-812.0986362
TACATGA7900.012.0330012
TCCTATA951.0329622E-612.0076682
GTAAAAA6350.011.981481
TGTACGC1459.640644E-1111.7994155
AAGCGGT1701.8189894E-1211.733410
TTAGGCA657.981814E-411.7002994
CGCAAAA6300.011.618532
CGCAGAA5250.011.5883532
GAGAACA27450.011.46779251
Produced by FastQC (version 0.11.5)