##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295030_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3382656
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.4831777159723	33.0	33.0	33.0	22.0	33.0
2	30.374230781965416	33.0	33.0	33.0	22.0	33.0
3	30.577075528815225	33.0	33.0	33.0	27.0	33.0
4	33.70937689200439	37.0	33.0	37.0	22.0	37.0
5	34.57465938008476	37.0	37.0	37.0	27.0	37.0
6	34.1231278025315	37.0	37.0	37.0	27.0	37.0
7	34.27098498931017	37.0	37.0	37.0	27.0	37.0
8	34.01600753963749	37.0	37.0	37.0	27.0	37.0
9	33.880743711450414	37.0	37.0	37.0	27.0	37.0
10	34.05379796231127	37.0	37.0	37.0	27.0	37.0
11	34.18811667518069	37.0	37.0	37.0	27.0	37.0
12	34.213019296079764	37.0	37.0	37.0	27.0	37.0
13	33.85525604731903	37.0	37.0	37.0	27.0	37.0
14	35.38507048898853	37.0	37.0	40.0	27.0	40.0
15	35.35334778351686	37.0	37.0	40.0	27.0	40.0
16	35.36402519203845	37.0	37.0	40.0	27.0	40.0
17	35.44075986443789	37.0	37.0	40.0	27.0	40.0
18	35.30199730625875	37.0	37.0	40.0	27.0	40.0
19	35.215749694914294	37.0	33.0	40.0	27.0	40.0
20	35.35429999385099	37.0	37.0	40.0	27.0	40.0
21	35.31312199644303	37.0	37.0	40.0	27.0	40.0
22	35.355137501419	37.0	37.0	40.0	27.0	40.0
23	35.3753509076891	37.0	33.0	40.0	27.0	40.0
24	35.51409277207023	37.0	33.0	40.0	27.0	40.0
25	34.389917567733754	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5971122516613931
1101	2	0.5223889179180183
1101	3	0.4887641165565988
1101	4	0.5184634877327383
1101	5	0.38777763514269026
1101	6	0.3262727323314678
1101	7	0.3217735706443392
1101	8	0.391266495577959
1101	9	0.4046831493107419
1101	10	0.5545042169063876
1101	11	0.3893731277779864
1101	12	0.4824890105843238
1101	13	0.4867316302835718
1101	14	0.9687336876567514
1101	15	0.8238771316007387
1101	16	0.8518821482424883
1101	17	0.773868411229131
1101	18	0.8508076951432813
1101	19	0.7966053940277931
1101	20	0.6534217983805632
1101	21	0.6797742154225617
1101	22	0.7461123356822554
1101	23	0.8571935322301272
1101	24	0.7576723233758145
1101	25	0.9892679967994127
1102	1	0.5429146493475905
1102	2	0.5632217863716846
1102	3	0.6148573730773208
1102	4	0.7460855729857983
1102	5	0.5226358466419327
1102	6	0.45131419101659276
1102	7	0.46696692537063456
1102	8	0.4833710780812126
1102	9	0.4231859672060594
1102	10	0.620804355200498
1102	11	0.45476435245277
1102	12	0.4388653259994868
1102	13	0.46684109497453363
1102	14	0.9460374001610816
1102	15	0.8936386299145838
1102	16	0.9823235374203136
1102	17	0.8018748104991147
1102	18	0.9074760610392403
1102	19	0.9339948823962487
1102	20	0.9489173269141418
1102	21	0.878493333306416
1102	22	0.8190391848731835
1102	23	0.961096667579362
1102	24	0.962443215213753
1102	25	1.0391894347667332
1103	1	0.4971083418529787
1103	2	0.46869472601692763
1103	3	0.42473487998280746
1103	4	0.47819199000839774
1103	5	0.333856784267887
1103	6	0.28311861451417997
1103	7	0.30719548097516025
1103	8	0.3817163174657736
1103	9	0.3719816844594064
1103	10	0.5648960637153806
1103	11	0.3302505044881059
1103	12	0.4410131269970634
1103	13	0.46657970831832074
1103	14	0.796150936522416
1103	15	0.6706248108585839
1103	16	0.6502136806824765
1103	17	0.6171757338684785
1103	18	0.693194838876714
1103	19	0.6892161858684105
1103	20	0.6367319018515261
1103	21	0.5281527479992363
1103	22	0.587293680369001
1103	23	0.7240158794074532
1103	24	0.6727570943815522
1103	25	0.7668716171249841
1104	1	0.4890528698302461
1104	2	0.47518153071906255
1104	3	0.40531418337038616
1104	4	0.454122841272401
1104	5	0.3211402771254299
1104	6	0.28984275922650937
1104	7	0.3282454440000677
1104	8	0.3724593237033531
1104	9	0.3396161340627515
1104	10	0.5292191396295607
1104	11	0.38785380114045864
1104	12	0.41810014717721344
1104	13	0.45778419400977555
1104	14	0.7895548218659982
1104	15	0.7743642804636863
1104	16	0.7405561963993463
1104	17	0.7217156132623899
1104	18	0.7375806630276429
1104	19	0.7182410279836517
1104	20	0.9024304729365156
1104	21	0.6289919141881555
1104	22	0.6814473385914326
1104	23	0.6871368160115736
1104	24	0.768081743208846
1104	25	0.808700892963131
1105	1	0.21002923784800487
1105	2	0.06042764100961051
1105	3	0.10799525304134505
1105	4	0.18125354603218113
1105	5	0.10767200235456187
1105	6	0.08104629413343645
1105	7	0.08334003694610459
1105	8	0.10094183215844055
1105	9	0.19238911214097243
1105	10	0.2040029463813653
1105	11	0.12010718484249594
1105	12	0.2425970172829892
1105	13	0.1984630319640317
1105	14	0.5566753903954904
1105	15	0.4098967300702867
1105	16	0.3564401655100369
1105	17	0.2909118115787024
1105	18	0.29183120573639343
1105	19	0.3208672581675174
1105	20	0.352815096008662
1105	21	0.3251750675069047
1105	22	0.3160916044648445
1105	23	0.45213422107229206
1105	24	0.392203811725814
1105	25	0.3889457431171621
1106	1	0.22422642027622075
1106	2	-0.010312231257891824
1106	3	-0.01641911628222914
1106	4	0.15678094520603025
1106	5	-0.23049933014533508
1106	6	-0.17961714835731613
1106	7	-0.4826375693350684
1106	8	-0.13349710644661172
1106	9	0.12056170644737563
1106	10	-0.24196818477486914
1106	11	-0.2737101863906446
1106	12	-0.5441323976081662
1106	13	-0.1634966997992109
1106	14	-0.2073415073999243
1106	15	-0.13264932772640492
1106	16	-0.020656038435575397
1106	17	-0.1374308415219332
1106	18	-0.42263239387281715
1106	19	-0.4436384966865319
1106	20	-0.12816062995770494
1106	21	-0.006120411135469794
1106	22	0.2906690542225121
1106	23	-0.3813171122407155
1106	24	0.1882237418232222
1106	25	0.37972724260438184
1107	1	-0.9498957599125433
1107	2	-1.0553434864855475
1107	3	-0.6985926376698437
1107	4	-0.7774219177617709
1107	5	-0.9518972193113768
1107	6	-0.8841032771158339
1107	7	-1.1187983874732126
1107	8	-0.9834762979227492
1107	9	-1.055331740777497
1107	10	-0.9075053774187154
1107	11	-0.9037835603608642
1107	12	-0.9700834016024231
1107	13	-0.7528473080911837
1107	14	-1.0349678518159422
1107	15	-1.005650906643723
1107	16	-1.0043127186924465
1107	17	-1.0873560117157979
1107	18	-1.113827213146969
1107	19	-1.11840743478556
1107	20	-1.2080666428563447
1107	21	-1.132901938291333
1107	22	-1.1732802682732526
1107	23	-0.8662738747947571
1107	24	-0.7937006698625098
1107	25	-0.8809750132446084
1108	1	-0.013427672984676065
1108	2	-0.1822921274878837
1108	3	-0.13895776418025463
1108	4	-0.27735161534501884
1108	5	-0.6425120752440634
1108	6	-0.2732678115067344
1108	7	-0.15240536690320994
1108	8	0.04833745040401993
1108	9	-0.35735279326908653
1108	10	-0.662934143893203
1108	11	-0.2809752814928572
1108	12	-0.017768041722618477
1108	13	-0.1922915768373059
1108	14	-0.4097888230753455
1108	15	-0.18123808420324394
1108	16	-0.8103099096433652
1108	17	-0.8053435118768348
1108	18	-0.7547566152522691
1108	19	-0.1350150127904186
1108	20	-1.017528877159961
1108	21	-0.24288968835494984
1108	22	-0.4495860819852808
1108	23	-0.22999260528410304
1108	24	-0.5131828755935643
1108	25	-1.1511711659915989
1109	1	0.10815104565944367
1109	2	0.0744618433847819
1109	3	0.11228211918164632
1109	4	0.15157197704400005
1109	5	0.025525048827432784
1109	6	0.08263270005916468
1109	7	0.14871889773758085
1109	8	0.2116861816830422
1109	9	0.21692009642099208
1109	10	0.2342969755159885
1109	11	0.1909675958842385
1109	12	0.27952618512077265
1109	13	0.2765734550718548
1109	14	0.48821407141653594
1109	15	0.29938759817522964
1109	16	0.3641485467528156
1109	17	0.3075531394723612
1109	18	0.299307208427841
1109	19	0.23087089218762458
1109	20	0.11003089718234094
1109	21	0.12831494605114102
1109	22	0.3239124841816192
1109	23	0.36756283670936796
1109	24	0.4565549971829199
1109	25	0.4154707539766349
1110	1	-0.01673557617363386
1110	2	-0.0016632595528065508
1110	3	0.13310213533844362
1110	4	-1.0460865272646984
1110	5	-0.2487475038028748
1110	6	-0.08031254171203273
1110	7	0.022772752856269562
1110	8	0.1414322406905839
1110	9	0.08920319664664333
1110	10	0.025910025457193342
1110	11	0.05889641984432359
1110	12	0.08108685598020315
1110	13	0.09041818538516111
1110	14	0.331892202709426
1110	15	0.16195942609881797
1110	16	0.13474774465530714
1110	17	0.2433532663253999
1110	18	0.196548742096752
1110	19	0.135738225050261
1110	20	0.2630858107147631
1110	21	0.2319727377902936
1110	22	-0.340697181706787
1110	23	0.07638744280588128
1110	24	0.26559461396145423
1110	25	0.25452795008073537
1111	1	-0.14785643367842738
1111	2	-0.3242215086947411
1111	3	-0.12390981221513186
1111	4	-0.1693778675496631
1111	5	-0.14047886829962408
1111	6	-0.10785143440190126
1111	7	-0.09562240642303266
1111	8	-0.019305704827274894
1111	9	-0.12834640392217977
1111	10	-0.05758803423115921
1111	11	-0.026597756983406384
1111	12	-0.04749820754185663
1111	13	-0.20220907782966435
1111	14	-0.2009537413338336
1111	15	-0.07821431628473619
1111	16	-0.23574262966404547
1111	17	-0.1824091898220601
1111	18	-0.20719154284111596
1111	19	-0.2071960930337653
1111	20	-0.10081270740965209
1111	21	-0.26080073837612616
1111	22	-0.18714622314035267
1111	23	-0.058484688141291485
1111	24	0.0507524428298467
1111	25	0.006817909035888192
1112	1	-0.06402192714370614
1112	2	-0.3747542966941779
1112	3	-0.11791612354788228
1112	4	-0.18942227360267339
1112	5	0.022391180004845523
1112	6	-0.06239761756904727
1112	7	-0.04301941767047879
1112	8	0.014613220399539273
1112	9	-0.14617486739683017
1112	10	0.2096436123013774
1112	11	0.05500926560535646
1112	12	0.06300621947028162
1112	13	-0.0011765999082982148
1112	14	-0.18970328032031603
1112	15	-0.06920223939768988
1112	16	-0.12979787639540064
1112	17	-0.2514175273060104
1112	18	-0.16268841410460055
1112	19	0.003271901727991633
1112	20	-0.8281944481453962
1112	21	-1.2332761796282554
1112	22	-1.6989089926376977
1112	23	-0.4738152277797383
1112	24	-0.25878472781158024
1112	25	-1.4953412243664204
1113	1	-0.3254236176893315
1113	2	-0.302776582414058
1113	3	-0.25694150507128555
1113	4	-0.757609692684774
1113	5	-0.4176842488364727
1113	6	-0.3139172553178753
1113	7	-0.31438180461128695
1113	8	-0.16621868394374673
1113	9	-0.26720501258288465
1113	10	-0.18817604961459722
1113	11	-0.2908584728644925
1113	12	-0.28690349253416514
1113	13	-0.32932239403485397
1113	14	-0.5925062862310995
1113	15	-0.54102420986559
1113	16	-0.5801151360270609
1113	17	-0.47724884691986347
1113	18	-0.9419629054213701
1113	19	-1.2538799916534984
1113	20	-1.3913466692765084
1113	21	-0.9993161171500446
1113	22	-0.40916950716446365
1113	23	-1.998453009230964
1113	24	-1.12894116677424
1113	25	-0.47532271128064707
1114	1	-0.399152363765797
1114	2	-0.5263424964394794
1114	3	-0.35529904714771376
1114	4	-0.5802799564155947
1114	5	-0.8122870105703655
1114	6	-0.6388981406623842
1114	7	-0.5481132878276682
1114	8	-0.3176836688035536
1114	9	-1.2927895184664067
1114	10	-0.3904508424085549
1114	11	-0.5404947290052391
1114	12	-0.3982481042798085
1114	13	-0.501110978543764
1114	14	-0.8314560986280597
1114	15	-1.529438978249317
1114	16	-1.4857714045429304
1114	17	-1.8321474834683116
1114	18	-1.9172525568135725
1114	19	-1.8827038269414018
1114	20	-1.0296147360557697
1114	21	-0.729521930355439
1114	22	-0.5649092587531399
1114	23	-0.3487580242249564
1114	24	-1.0329816437263588
1114	25	-0.6828679329189384
1115	1	-0.9852898681645037
1115	2	-0.9129643135014867
1115	3	-0.765514051752664
1115	4	-1.086652334640803
1115	5	-0.7455479906135523
1115	6	-0.8452581848117973
1115	7	-0.8023380812319019
1115	8	-0.6470547656380958
1115	9	-0.9720208342813805
1115	10	-0.6929722639421385
1115	11	-0.8276881283690187
1115	12	-0.9877214889022881
1115	13	-0.8172215161958718
1115	14	-0.8634948692155504
1115	15	-1.982370978012689
1115	16	-1.3039166922586816
1115	17	-1.0753483009065476
1115	18	-2.622941023881623
1115	19	-1.69525833942852
1115	20	-1.372239874317529
1115	21	-1.2653004647307995
1115	22	-1.1523375580144446
1115	23	-0.7830516029139076
1115	24	-0.9133427844725688
1115	25	-0.9158756640318657
1116	1	-1.6272244430089167
1116	2	-0.9647936037296816
1116	3	-1.7819946516431422
1116	4	-1.2367204858445433
1116	5	-1.2245248431871119
1116	6	-0.6815673874227244
1116	7	-1.5033437611650697
1116	8	-3.1785076453762002
1116	9	-1.7508653815855126
1116	10	-1.0002508524727531
1116	11	-0.7942178930575068
1116	12	-0.9855562392210615
1116	13	-1.9372787552127342
1116	14	-1.8244236442126507
1116	15	-1.698045355158655
1116	16	-1.3046983326041897
1116	17	-1.986058632309259
1116	18	-2.6928973351627477
1116	19	-1.6085000583577695
1116	20	-1.0633144649106612
1116	21	-0.9355495663740214
1116	22	-0.6747623018978217
1116	23	-0.6792119521038842
1116	24	-0.6454058273338816
1116	25	-0.603793714707578
1201	1	0.4825769313992012
1201	2	0.44572927870331114
1201	3	0.49595520832918893
1201	4	0.560654417148335
1201	5	0.43823173361440837
1201	6	0.22087550490780217
1201	7	0.2285600527612175
1201	8	0.34433598477080096
1201	9	0.36098185891341217
1201	10	0.5597861508226813
1201	11	0.3558225968706239
1201	12	0.40146673881314854
1201	13	0.464038292171729
1201	14	0.9154049484249285
1201	15	0.8394539418912075
1201	16	0.8292114198448886
1201	17	0.7824278316828241
1201	18	0.6623837886576993
1201	19	0.6182199200476006
1201	20	0.5943550023093636
1201	21	0.6137036193960199
1201	22	0.7320737476348569
1201	23	0.8861714087850956
1201	24	0.7163671816301402
1201	25	0.9463401269992957
1202	1	0.6125125721423927
1202	2	0.6064866210195134
1202	3	0.5699790513767375
1202	4	0.7936946892404393
1202	5	0.5586712571073704
1202	6	0.40770029675469743
1202	7	0.43459485413551135
1202	8	0.41571133762465706
1202	9	0.46728000116976887
1202	10	0.6567275679328404
1202	11	0.4949091723975414
1202	12	0.43594420543204393
1202	13	0.5495382959923418
1202	14	0.9739632656815189
1202	15	0.8411734999386056
1202	16	0.8012281935490861
1202	17	0.9207006967535989
1202	18	0.9960143255827347
1202	19	0.9604396809897437
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2210	23	0.7217742605451534
2210	24	0.7450759121947641
2210	25	0.7317708634125637
2211	1	-0.11356024560080868
2211	2	-0.3703850657296144
2211	3	-0.0808548982375541
2211	4	-0.23729098400502835
2211	5	-0.14894150156767694
2211	6	-0.2256142370465355
2211	7	-0.08210813755299284
2211	8	0.010053804360296681
2211	9	0.026029650067400212
2211	10	0.13042637068856777
2211	11	0.08489159950157443
2211	12	0.06256157258497552
2211	13	-0.15815890502546637
2211	14	-0.10872438001855755
2211	15	-0.11977110173648953
2211	16	-0.1454120974777453
2211	17	-0.0876081334858867
2211	18	-0.1320993006262725
2211	19	-0.1503415617882169
2211	20	-0.1236095330224245
2211	21	-0.12493258317823575
2211	22	-0.12128835340423905
2211	23	-0.08784109929820971
2211	24	-0.03238090273741534
2211	25	-0.27522779047570367
2212	1	-0.05223486873603633
2212	2	0.03661794042972488
2212	3	0.0648599561310732
2212	4	-0.16732446311050353
2212	5	0.04686199696365634
2212	6	0.04350916720212439
2212	7	0.03873884236736558
2212	8	0.10450675999990011
2212	9	-0.03190148041743157
2212	10	0.18696879476036088
2212	11	0.07824289627925651
2212	12	0.1532298486886674
2212	13	0.015798323677252313
2212	14	0.05209126358420235
2212	15	-0.032026870840930144
2212	16	-0.06850517267552192
2212	17	0.04160588566469414
2212	18	0.07070833802148968
2212	19	0.06886876613653214
2212	20	0.021456228668277788
2212	21	0.0010848500434192943
2212	22	-0.7006010268214666
2212	23	-0.06745411493587028
2212	24	0.016432039701363976
2212	25	0.1506311624674268
2213	1	-0.005933414389627245
2213	2	-0.642157342094702
2213	3	-0.12654260594077726
2213	4	0.016199519212428015
2213	5	0.13472664624855923
2213	6	0.07211471880524556
2213	7	0.018423333763742278
2213	8	0.29474335416551156
2213	9	0.01652664420503669
2213	10	-1.5015818627605242
2213	11	-0.2616328332430342
2213	12	-0.011205743796040224
2213	13	-0.07693527633348651
2213	14	-0.0025522492791054674
2213	15	0.044425685787004454
2213	16	0.051467219948790444
2213	17	-0.05146777278240222
2213	18	0.0673821414408664
2213	19	-0.015911679906778886
2213	20	-0.07826255195375609
2213	21	0.1225127449772998
2213	22	0.14287980685863744
2213	23	-0.8983130756993916
2213	24	-1.4714966315501314
2213	25	-1.3579100092128655
2214	1	0.006913717914688533
2214	2	-0.20418932323487482
2214	3	-0.008588573081176776
2214	4	-0.046464469529915675
2214	5	-0.1152454715151805
2214	6	-0.1419707475345291
2214	7	-0.03757305009159495
2214	8	0.045755291820462674
2214	9	-0.3064132858379125
2214	10	0.03061252887059851
2214	11	-0.07889997322526199
2214	12	0.028033784964044628
2214	13	-0.07544485245217203
2214	14	0.009936164375972112
2214	15	-0.08144348989974048
2214	16	-0.16756339308659562
2214	17	-0.16408031311173943
2214	18	-0.094571628028433
2214	19	-0.8559030576249498
2214	20	-0.31458285668969665
2214	21	-0.3084063555903285
2214	22	-0.46178970840730216
2214	23	-1.7950607588607852
2214	24	-1.2254379562582258
2214	25	-1.6452685475959186
2215	1	-0.5691275145611208
2215	2	-0.3214906827725237
2215	3	-1.256832566390699
2215	4	-1.1282705391395424
2215	5	-0.45353052472748345
2215	6	-0.4251323828755389
2215	7	-0.40159333822768417
2215	8	-0.29086475121322763
2215	9	-0.45837117558322404
2215	10	-1.5503124595370252
2215	11	-0.5699412871177998
2215	12	-0.6630970416775241
2215	13	-0.6682328823970991
2215	14	-0.902487617770511
2215	15	-1.2263523343589355
2215	16	-0.8515915763628072
2215	17	-0.7209125844513267
2215	18	-0.7128541124287651
2215	19	-1.1599859620747281
2215	20	-1.3686884589064192
2215	21	-0.7656553740621916
2215	22	-0.4072702961478285
2215	23	-0.6808196031145357
2215	24	-1.913817049598478
2215	25	-0.8397932648782245
2216	1	-0.5674890286005336
2216	2	-0.43600278448570506
2216	3	-1.5553981317658732
2216	4	-0.9159360806342107
2216	5	-0.6752605666328577
2216	6	-0.6428120236803849
2216	7	-0.46195695431207895
2216	8	-0.6402021957938686
2216	9	-0.4007136113913248
2216	10	-0.49985892471643467
2216	11	-0.4760012799863631
2216	12	-0.47451918088138
2216	13	-1.1972600923054628
2216	14	-0.8715369603712873
2216	15	-1.2971077853989996
2216	16	-1.1580500137741652
2216	17	-1.9993009169333433
2216	18	-0.9645146329427092
2216	19	-0.8065836915109728
2216	20	-0.8819133189920692
2216	21	-0.5236297404538988
2216	22	-0.46541248031495286
2216	23	-0.40654647466126903
2216	24	-0.6230822015278505
2216	25	-0.5489193709092106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4024.0
3	469.0
4	523.0
5	449.0
6	343.0
7	221.0
8	309.0
9	626.0
10	1229.0
11	2471.0
12	4802.0
13	8764.0
14	15601.0
15	26897.0
16	35720.0
17	37149.0
18	34905.0
19	33276.0
20	32381.0
21	33106.0
22	32454.0
23	30583.0
24	30446.0
25	30432.0
26	29608.0
27	31027.0
28	33223.0
29	37440.0
30	44851.0
31	57685.0
32	76932.0
33	110269.0
34	162815.0
35	265352.0
36	496257.0
37	1640017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.849971748380945	18.036242706605897	10.454433073307763	23.659352471705397
2	10.610676828475649	25.717148704105814	33.55591161500334	30.116262852415197
3	23.02111142241622	33.6184045685637	24.528604760124935	18.83187924889515
4	13.262958708326645	23.69953771478371	26.806825446007775	36.23067813088187
5	9.582917400382762	44.14492333648302	33.71793242137719	12.554226841757032
6	31.94494667831158	29.190977500613783	19.71352086622533	19.1505549548493
7	21.23514287186577	36.97924162975151	22.138520230926282	19.64709526745644
8	28.912755192564994	34.998040597400085	21.623392453086154	14.46581175694877
9	28.35391347542898	20.137950686450694	17.654915344807108	33.853220493313216
10	17.593711442668162	35.60319404678499	28.93788020399004	17.8652143065568
11	24.311696189095183	31.94156170329722	15.14914039028366	28.597601717323933
12	25.084228188416418	37.082557693532316	24.723146572597663	13.110067545453601
13	30.25269851275578	23.687667754494363	18.876629556901527	27.18300417584833
14	21.005900685323045	33.348202041455856	22.69154420715724	22.954353066063863
15	38.241281902287646	21.828103925920527	19.086907615678093	20.84370655611373
16	15.258533268254773	29.706654835637146	35.38842186070977	19.646390035398316
17	27.965181798930516	32.47952327852636	25.24390659268243	14.311388329860694
18	28.542771225824833	24.607127170281963	28.42373725212485	18.426364351768346
19	22.5888817247167	35.35833909149148	29.45160911026016	12.601170073531657
20	24.660955254682655	28.58854317530102	25.499748548601204	21.25075302141513
21	17.322402753179368	32.38590121014317	31.064369191401646	19.227326845275822
22	21.190688802898975	31.699712560697673	16.905370124379328	30.204228512024027
23	14.428023820567365	33.169275188212225	26.52049231068152	25.88220868053889
24	20.219830350570312	42.05045641236038	19.778362662314102	17.951350574755203
25	21.09909861342687	25.7917234519572	17.590530390658117	35.51864754395781
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24427.0
1	24427.0
2	26026.5
3	27626.0
4	27626.0
5	27626.0
6	34528.0
7	41430.0
8	41430.0
9	41430.0
10	40340.5
11	39251.0
12	39251.0
13	39251.0
14	39612.0
15	39973.0
16	39973.0
17	39973.0
18	43861.5
19	47750.0
20	47750.0
21	47750.0
22	55319.5
23	62889.0
24	62889.0
25	62889.0
26	73474.5
27	84060.0
28	84060.0
29	84060.0
30	92367.5
31	100675.0
32	100675.0
33	100675.0
34	106510.5
35	112346.0
36	112346.0
37	112346.0
38	121836.5
39	131327.0
40	131327.0
41	131327.0
42	187624.5
43	243922.0
44	243922.0
45	243922.0
46	738477.5
47	1233033.0
48	1233033.0
49	1233033.0
50	1015391.0
51	797749.0
52	797749.0
53	797749.0
54	517012.5
55	236276.0
56	236276.0
57	236276.0
58	153834.0
59	71392.0
60	71392.0
61	71392.0
62	58133.0
63	44874.0
64	44874.0
65	44874.0
66	34953.5
67	25033.0
68	25033.0
69	25033.0
70	18501.5
71	11970.0
72	11970.0
73	11970.0
74	8088.5
75	4207.0
76	4207.0
77	4207.0
78	2921.5
79	1636.0
80	1636.0
81	1636.0
82	1100.0
83	564.0
84	564.0
85	564.0
86	352.5
87	141.0
88	141.0
89	141.0
90	104.5
91	68.0
92	68.0
93	68.0
94	44.0
95	20.0
96	20.0
97	20.0
98	18.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2778290195633254
2	0.1645748193135808
3	0.18299229954213495
4	0.20155759261361486
5	0.22801609149733226
6	0.17959260415484163
7	0.17719803609944376
8	0.19591114201384946
9	0.19709364475725583
10	0.206967542664699
11	0.18447042797139288
12	0.18952562719945512
13	0.20146890490785938
14	0.1903238165512544
15	0.18251929844477238
16	0.18399742687403034
17	0.18509124191168125
18	0.18651024520376888
19	0.18585986869489537
20	0.18500255420592576
21	0.1831696749536459
22	0.18597811896923602
23	0.18961431490521058
24	0.2031244087486283
25	0.19614764256253073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3382656.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.364181146501963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78513224466923	16.04394704681557
2	10.059540309527245	4.097086022275025
3	3.948537034531683	2.412261703046246
4	2.107980453119178	1.7170918320241695
5	1.2965715637240938	1.320180909654038
6	0.8182296642127638	0.999754626088212
7	0.5546411884116816	0.7906369542488627
8	0.4179312675019943	0.6808662430558224
9	0.298115951225274	0.5463798510071903
>10	1.472092802315431	5.376377114383192
>50	0.11355422536124907	1.6019439261467487
>100	0.09619226651514931	4.015574047411123
>500	0.011222403815548376	1.597014900574152
>1k	0.01209687684013656	5.175874005930836
>5k	0.003060655586058648	4.181271598609611
>10k+	0.00510109264343108	49.44373921872919
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	258514	7.642337855223824	No Hit
CCCATGTACTCTGCGTTGATACCAC	247182	7.307334828016801	No Hit
GAGTACATGGGAAGCAGTGGTATCA	117746	3.4808742006281452	No Hit
CATGTACTCTGCGTTGATACCACTG	92852	2.7449436182691946	No Hit
GCGTTGATACCACTGCTTCCCATGT	79396	2.3471496953872935	No Hit
GTATCAACGCAGAGTACATGGGAAG	65938	1.9492966473682218	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	64956	1.9202662050175956	No Hit
TATCAACGCAGAGTACATGGGAAGC	64475	1.906046609528134	No Hit
ACTCTGCGTTGATACCACTGCTTCC	57585	1.7023605119763876	No Hit
GGTATCAACGCAGAGTACATGGGAA	55409	1.6380323627350817	No Hit
GCTTCCCATGTACTCTGCGTTGATA	43622	1.289578366821811	No Hit
GTGGTATCAACGCAGAGTACATGGG	30169	0.8918731316456654	No Hit
ATACCACTGCTTCCCATGTACTCTG	29423	0.8698194554811366	No Hit
GTACTCTGCGTTGATACCACTGCTT	29358	0.8678978885231013	No Hit
CAGTGGTATCAACGCAGAGTACATG	27734	0.8198882771408029	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	26092	0.7713465395239717	No Hit
ACATGGGAAGCAGTGGTATCAACGC	24787	0.7327673875203391	No Hit
CATGGGAAGCAGTGGTATCAACGCA	24330	0.7192572936769213	No Hit
GCAGTGGTATCAACGCAGAGTACAT	24305	0.7185182294622924	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	23876	0.7058358875392591	No Hit
TACCACTGCTTCCCATGTACTCTGC	23652	0.6992138721761835	No Hit
GATACCACTGCTTCCCATGTACTCT	23122	0.6835457108260491	No Hit
GTTGATACCACTGCTTCCCATGTAC	23001	0.6799686400272449	No Hit
CCATGTACTCTGCGTTGATACCACT	21643	0.6398226718885988	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	21576	0.6378419797933932	No Hit
CTTCCCATGTACTCTGCGTTGATAC	20200	0.5971638854202141	No Hit
CCACTGCTTCCCATGTACTCTGCGT	19505	0.5766179002535285	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	18249	0.5394873141105687	No Hit
GTACATGGTAAGCAGTGGTATCAAC	17208	0.5087126802134181	No Hit
TTCCCATGTACTCTGCGTTGATACC	16680	0.49310364400045414	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	16643	0.4920098289628032	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	16291	0.48160380482082715	No Hit
TGATACCACTGCTTCCCATGTACTC	14468	0.42771124229008206	No Hit
ACCATGTACTCTGCGTTGATACCAC	13357	0.3948672285919703	No Hit
ATCAACGCAGAGTACATGGGAAGCA	12557	0.371217173723843	No Hit
CCCCATGTACTCTGCGTTGATACCA	9130	0.2699062511825028	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	8986	0.26564924130623985	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8797	0.2600619158436448	No Hit
TCCATGTACTCTGCGTTGATACCAC	8156	0.24111230938055778	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	7767	0.2296124702009309	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7526	0.22248789117190754	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7266	0.21480162333976616	No Hit
AAAAAGTACTCTGCGTTGATACCAC	7203	0.21293918151890112	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6485	0.19171325727475688	No Hit
GAGTACATGGTAAGCAGTGGTATCA	6235	0.1843226151284671	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6221	0.18390873916827485	No Hit
CTGCGTTGATACCACTGCTTCCCAT	6041	0.17858747682294623	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	5995	0.17722759866802892	No Hit
CTCTGCGTTGATACCACTGCTTCCC	5935	0.17545384455291935	No Hit
GTACATGGAAAGCAGTGGTATCAAC	5927	0.17521734400423808	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5821	0.17208371173421122	No Hit
TCCCATGTACTCTGCGTTGATACCA	5810	0.1717585234797745	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	5516	0.1630671283157377	No Hit
TCAACGCAGAGTACATGGGAAGCAG	5491	0.1623280641011087	No Hit
GCTTACCATGTACTCTGCGTTGATA	5451	0.16114556135770236	No Hit
AAAGTACTCTGCGTTGATACCACTG	5120	0.15136035115601468	No Hit
AGTGGTATCAACGCAGAGTACATGG	4497	0.13294287092746054	No Hit
ACTCTGCGTTGATACCACTGCTTAC	4456	0.13173080561546904	No Hit
GCGTTGATACCACTGCTTACCATGT	4312	0.1274737957392061	No Hit
TTGATACCACTGCTTCCCATGTACT	4175	0.1234237238430393	No Hit
GAGTACATGGAAAGCAGTGGTATCA	4047	0.11963971506413894	No Hit
AACGCAGAGTACATGGGAAGCAGTG	4021	0.11887108828092481	No Hit
GTACATGGAAGCAGTGGTATCAACG	3873	0.11449582813032126	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	3820	0.11292901199530783	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	3819	0.11289944942672267	No Hit
CTGCTTACCATGTACTCTGCGTTGA	3668	0.10843550157036365	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3604	0.10654349718091345	No Hit
GTATCAACGCAGAGTACATGGTAAG	3578	0.10577487039769932	No Hit
TATCAACGCAGAGTACATGGTAAGC	3438	0.10163611079577704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3431	0.10142917281568092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.9125137170318236E-5	0.0
2	0.0	0.0	0.0	5.9125137170318236E-5	0.0
3	0.0	2.9562568585159118E-5	0.0	5.9125137170318236E-5	0.0
4	0.0	2.9562568585159118E-5	0.0	5.9125137170318236E-5	0.0
5	0.0	2.9562568585159118E-5	0.0	5.9125137170318236E-5	0.0
6	0.0	2.9562568585159118E-5	0.0	5.9125137170318236E-5	0.0
7	0.0	2.9562568585159118E-5	0.0	5.9125137170318236E-5	0.0
8	2.9562568585159118E-5	2.9562568585159118E-5	0.0	1.773754115109547E-4	0.0
9	2.9562568585159118E-5	2.9562568585159118E-5	0.0	2.0693798009611383E-4	0.0
10	2.9562568585159118E-5	2.9562568585159118E-5	0.0	5.616888031180232E-4	0.0
11	2.9562568585159118E-5	2.9562568585159118E-5	0.0	6.208139402883414E-4	0.0
12	2.9562568585159118E-5	2.9562568585159118E-5	0.0	9.164396261399327E-4	0.0
13	2.9562568585159118E-5	2.9562568585159118E-5	0.0	0.0010346899004805691	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	1325	0.0	17.082651	1
GACTAGA	45	3.5081168E-5	16.9072	1
TATGCGT	45	6.773516E-4	14.775393	10
CGCCGGC	40	0.0052937325	14.244747	19
TACATAA	920	0.0	13.639145	2
GTACATA	1180	0.0	13.459491	1
AGAACAA	1645	0.0	13.291162	2
GTCTTGG	105	1.9665094E-8	12.6804	1
TACAAAA	1880	0.0	12.38823	2
GTACAAG	435	0.0	12.243144	1
TATTAGG	110	3.785499E-8	12.098636	2
TACATGA	790	0.0	12.033001	2
TCCTATA	95	1.0329622E-6	12.007668	2
GTAAAAA	635	0.0	11.98148	1
TGTACGC	145	9.640644E-11	11.799415	5
AAGCGGT	170	1.8189894E-12	11.7334	10
TTAGGCA	65	7.981814E-4	11.700299	4
CGCAAAA	630	0.0	11.61853	2
CGCAGAA	525	0.0	11.588353	2
GAGAACA	2745	0.0	11.4677925	1
>>END_MODULE