FastQCFastQC Report
Wed 25 May 2016
SRR1295030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382656
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2767198.180524416316645No Hit
GTACATGGGAAGCAGTGGTATCAAC2463977.2841282116774515No Hit
GAGTACATGGGAAGCAGTGGTATCA1228773.6325597400385967No Hit
CATGTACTCTGCGTTGATACCACTG1040923.0772268891663828No Hit
GCGTTGATACCACTGCTTCCCATGT860042.542499148598025No Hit
ACGCAGAGTACATGGGAAGCAGTGG777702.2990809588678247No Hit
TATCAACGCAGAGTACATGGGAAGC681462.0145707988042534No Hit
GTATCAACGCAGAGTACATGGGAAG669991.980662532637076No Hit
ACTCTGCGTTGATACCACTGCTTCC644171.904331980550195No Hit
GGTATCAACGCAGAGTACATGGGAA579111.7119979093351496No Hit
GCTTCCCATGTACTCTGCGTTGATA478641.4149827827600558No Hit
CAGTGGTATCAACGCAGAGTACATG326040.9638579861505279No Hit
GTGGTATCAACGCAGAGTACATGGG313280.9261361486358649No Hit
ATACCACTGCTTCCCATGTACTCTG311020.9194550081356189No Hit
ACATGGGAAGCAGTGGTATCAACGC306030.9047032864116246No Hit
CATGGGAAGCAGTGGTATCAACGCA303380.8968692057365573No Hit
GGGAAGCAGTGGTATCAACGCAGAG294490.8705880822643508No Hit
GTACTCTGCGTTGATACCACTGCTT287310.8493621580202066No Hit
GTATCAACGCAGAGTACTTTTTTTT279400.8259781662693458No Hit
GCAGTGGTATCAACGCAGAGTACAT278650.8237609736254589No Hit
CTGCTTCCCATGTACTCTGCGTTGA260260.7693954099973512No Hit
GATACCACTGCTTCCCATGTACTCT258810.7651088375525031No Hit
CCATGTACTCTGCGTTGATACCACT254740.7530768721383433No Hit
TACCACTGCTTCCCATGTACTCTGC248820.7355758315359292No Hit
GCAGAGTACATGGGAAGCAGTGGTA241750.7146750955462217No Hit
GTTGATACCACTGCTTCCCATGTAC226790.6704494929428236No Hit
CTTCCCATGTACTCTGCGTTGATAC225900.6678184243387445No Hit
GGTATCAACGCAGAGTACTTTTTTT225210.6657786071063685No Hit
CCACTGCTTCCCATGTACTCTGCGT223990.6621719737389791No Hit
TATCAACGCAGAGTACTTTTTTTTT218570.6461490615658229No Hit
GTACTTTTTTTTTTTTTTTTTTTTT205470.6074220967192644No Hit
AAGCAGTGGTATCAACGCAGAGTAC205280.6068604079161464No Hit
ATGGGAAGCAGTGGTATCAACGCAG193170.5710601373595187No Hit
GTACATGGTAAGCAGTGGTATCAAC173380.5125558141294888No Hit
TTCCCATGTACTCTGCGTTGATACC162350.47994830098005825No Hit
ACCATGTACTCTGCGTTGATACCAC150910.4461287225186362No Hit
TGATACCACTGCTTCCCATGTACTC142950.4225969179248496No Hit
ATCAACGCAGAGTACATGGGAAGCA141860.41937459794906723No Hit
ACGCAGAGTACTTTTTTTTTTTTTT139020.410978828470882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA113580.33577165399023723No Hit
GAGTACTTTTTTTTTTTTTTTTTTT106680.31537348166647744No Hit
CCCCATGTACTCTGCGTTGATACCA102050.3016860124115488No Hit
GGAAGCAGTGGTATCAACGCAGAGT90960.2689011238506073No Hit
GGTAAGCAGTGGTATCAACGCAGAG86120.25459284065539034No Hit
TCCATGTACTCTGCGTTGATACCAC83830.24782301244938887No Hit
GTACATGGGTAAGCAGTGGTATCAA78650.23250960192227643No Hit
AAAAAGTACTCTGCGTTGATACCAC71100.2101898626404813No Hit
CTGCGTTGATACCACTGCTTCCCAT69570.205666789646952No Hit
GAGTACATGGTAAGCAGTGGTATCA67880.2006707155560601No Hit
CTCTGCGTTGATACCACTGCTTCCC66060.19529032807356114No Hit
TCAACGCAGAGTACATGGGAAGCAG63590.1879883736330268No Hit
TGGGAAGCAGTGGTATCAACGCAGA63460.18760406024141976No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA62770.18556424300904378No Hit
TCCCATGTACTCTGCGTTGATACCA60500.17885353994021266No Hit
GCTTACCATGTACTCTGCGTTGATA59780.1767250350020812No Hit
GTACATGGAAAGCAGTGGTATCAAC59490.1758677205131116No Hit
AAAGTACTCTGCGTTGATACCACTG54270.16043605971165853No Hit
AGTGGTATCAACGCAGAGTACATGG48760.14414708442123586No Hit
GCGTTGATACCACTGCTTACCATGT48250.14263939342339274No Hit
AACGCAGAGTACATGGGAAGCAGTG47990.1418707666401786No Hit
ACTCTGCGTTGATACCACTGCTTAC47500.14042220077950582No Hit
ACGCAGAGTACATGGTAAGCAGTGG45480.13445056192530366No Hit
GAGTACATGGAAAGCAGTGGTATCA43700.12918842471714537No Hit
GCAGAGTACTTTTTTTTTTTTTTTT43520.12865629848261248No Hit
TTGATACCACTGCTTCCCATGTACT40230.11893021341809512No Hit
ACTGCTTCCCATGTACTCTGCGTTG40230.11893021341809512No Hit
CAACGCAGAGTACATGGGAAGCAGT40080.11848677488931773No Hit
GTGGTATCAACGCAGAGTACTTTTT39730.11745208498883718No Hit
CTGCTTACCATGTACTCTGCGTTGA39120.11564876830514248No Hit
ATGGTAAGCAGTGGTATCAACGCAG37440.11068225678283573No Hit
TATCAACGCAGAGTACATGGTAAGC37320.11032750595981382No Hit
GTACATGGAAGCAGTGGTATCAACG36280.10725299882695727No Hit
CGTTGATACCACTGCTTCCCATGTA35790.10580443296628447No Hit
CGCAGAGTACATGGGAAGCAGTGGT35520.10500624361448518No Hit
GCTTTCCATGTACTCTGCGTTGATA34200.10110398456124418No Hit
GTATCAACGCAGAGTACATGGTAAG33930.10030579520944488No Hit
ATGTACTCTGCGTTGATACCACTGC33910.10024667007227457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA6750.017.3337821
GGACGCA1450.016.3782168
GATACGA350.002171879916.28462618
CGCAAAA3200.015.4369242
TGGGACG1700.015.0872276
GGGACGC1650.014.9686967
TAAGCTG456.768671E-414.7767895
ACGCAGT1750.013.5703210
GAACAAA12050.013.4200411
CGCCGAG1001.009721E-813.2991118
GTACAAG3600.012.6832551
TATACTG1352.7284841E-1112.6658195
ACGCAAA5350.012.4461841
AAAAAGT12000.012.4454441
GCCGAGT1001.4458965E-712.3489919
CGCAGTG2000.012.34899111
AAAAGTA12400.012.0277772
TACAAAA9600.011.9735122
ACGCCGA1052.725683E-711.7611197
CTATGCG658.033206E-411.6913539
Produced by FastQC (version 0.11.5)