FastQCFastQC Report
Wed 25 May 2016
SRR1295029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038228
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2318757.6319157087618175No Hit
CCCATGTACTCTGCGTTGATACCAC2237817.365510422522601No Hit
GAGTACATGGGAAGCAGTGGTATCA1056293.476664687442812No Hit
CATGTACTCTGCGTTGATACCACTG826872.721553484465287No Hit
GCGTTGATACCACTGCTTCCCATGT724382.38421869589774No Hit
GTATCAACGCAGAGTACATGGGAAG600141.9752961265579805No Hit
ACGCAGAGTACATGGGAAGCAGTGG593811.9544616138091018No Hit
TATCAACGCAGAGTACATGGGAAGC579871.9085796062704972No Hit
ACTCTGCGTTGATACCACTGCTTCC533861.7571426502553462No Hit
GGTATCAACGCAGAGTACATGGGAA492901.6223272249482266No Hit
GCTTCCCATGTACTCTGCGTTGATA405611.3350215981157438No Hit
GTACTCTGCGTTGATACCACTGCTT269300.8863719246876798No Hit
ATACCACTGCTTCCCATGTACTCTG268540.8838704666009266No Hit
GTGGTATCAACGCAGAGTACATGGG268030.8821918565690263No Hit
CAGTGGTATCAACGCAGAGTACATG248900.8192275234116728No Hit
GGGAAGCAGTGGTATCAACGCAGAG234350.7713377666192267No Hit
ACATGGGAAGCAGTGGTATCAACGC226500.7455003376968418No Hit
CATGGGAAGCAGTGGTATCAACGCA223090.7342766902286464No Hit
TACCACTGCTTCCCATGTACTCTGC218780.7200907897629802No Hit
GCAGTGGTATCAACGCAGAGTACAT218540.7193008556303213No Hit
CTGCTTCCCATGTACTCTGCGTTGA217070.7144625090677856No Hit
GATACCACTGCTTCCCATGTACTCT212470.6993221048584899No Hit
GTTGATACCACTGCTTCCCATGTAC204560.6732871924029401No Hit
CCATGTACTCTGCGTTGATACCACT195220.6425455890736311No Hit
GCAGAGTACATGGGAAGCAGTGGTA192850.6347449895136243No Hit
CTTCCCATGTACTCTGCGTTGATAC184560.607459348014698No Hit
CCACTGCTTCCCATGTACTCTGCGT180070.5926809969495377No Hit
AAGCAGTGGTATCAACGCAGAGTAC164030.5398870657501675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA156950.5165840088367298No Hit
TTCCCATGTACTCTGCGTTGATACC154780.5094416877206055No Hit
ATGGGAAGCAGTGGTATCAACGCAG152260.501147379327687No Hit
GTACATGGTAAGCAGTGGTATCAAC148660.48929836733780346No Hit
TGATACCACTGCTTCCCATGTACTC133240.4385450993144688No Hit
ACCATGTACTCTGCGTTGATACCAC117450.3865740161699517No Hit
ATCAACGCAGAGTACATGGGAAGCA113360.3731122219925562No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA94460.3109049090456674No Hit
CCCCATGTACTCTGCGTTGATACCA85260.28062410062707605No Hit
TCCATGTACTCTGCGTTGATACCAC77560.2552803805376028No Hit
GGAAGCAGTGGTATCAACGCAGAGT69960.23026579967007085No Hit
GTATCAACGCAGAGTACTTTTTTTT69290.22806056688306472No Hit
GGTAAGCAGTGGTATCAACGCAGAG66450.21871301297993434No Hit
AAAAAGTACTCTGCGTTGATACCAC64900.21361135503984557No Hit
GTACATGGGTAAGCAGTGGTATCAA64330.21173526147478067No Hit
GAACAAAAAAAAAAAAAAAAAAAAA60650.19962293810734413No Hit
CTGCGTTGATACCACTGCTTCCCAT57530.18935379438277838No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55550.18283683778834242No Hit
CTCTGCGTTGATACCACTGCTTCCC55230.18178359227813054No Hit
GAGTACATGGTAAGCAGTGGTATCA53610.17645153688268292No Hit
GTACATGGAAAGCAGTGGTATCAAC53500.1760894837385476No Hit
TCCCATGTACTCTGCGTTGATACCA52880.17404882056251209No Hit
TCAACGCAGAGTACATGGGAAGCAG50960.16772934750124086No Hit
GGTATCAACGCAGAGTACTTTTTTT50170.1651291476479053No Hit
TGGGAAGCAGTGGTATCAACGCAGA49970.16447086920402287No Hit
TATCAACGCAGAGTACTTTTTTTTT49040.16140987443996963No Hit
GCTTACCATGTACTCTGCGTTGATA48240.15877676066443994No Hit
AAAGTACTCTGCGTTGATACCACTG47290.15564993805599844No Hit
AGTGGTATCAACGCAGAGTACATGG41540.13672443279437882No Hit
TTGATACCACTGCTTCCCATGTACT39560.13020747619994286No Hit
GCGTTGATACCACTGCTTACCATGT39080.12862760793462505No Hit
ACTCTGCGTTGATACCACTGCTTAC38490.1266856865251719No Hit
GAGTACATGGAAAGCAGTGGTATCA37110.12214356526238321No Hit
AACGCAGAGTACATGGGAAGCAGTG34930.11496833022406482No Hit
ACTGCTTCCCATGTACTCTGCGTTG33390.10989958620617017No Hit
GTACATGGAAGCAGTGGTATCAACG32880.10822097617427No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32760.10782600910794055No Hit
ACGCAGAGTACATGGTAAGCAGTGG32440.10677276359772868No Hit
CTGCTTACCATGTACTCTGCGTTGA32370.10654236614236984No Hit
GTATCAACGCAGAGTACATGGTAAG30850.10153944996886342No Hit
TATCAACGCAGAGTACATGGTAAGC30820.10144070820228106No Hit
ATGTACTCTGCGTTGATACCACTGC30680.10097991329156339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA350.002162823716.2947374
GTACAAA7800.015.1199131
GTACATA10050.014.3846641
TACATAA7400.013.4865312
AGAACAA15950.013.05046752
GGACCGT751.4820469E-512.66476
AGGCTAG701.0943231E-412.210978518
TACATGA6950.011.8980862
ATGCGTT658.043314E-411.689527511
CGCAAAA7350.011.6385222
TAGAACC1001.9185572E-611.4063154
AGGACTC752.0650416E-411.405945
GTACAAG4100.011.36671
ACATAAA15350.011.3314473
GAGAACA24200.011.1615661
CGCAGAA4700.011.1226292
GGTTTAG600.00582783111.0960651
CTATTCC1201.2669989E-711.0894744
CCTACGC600.005855819711.0889243
AAAAGTA13300.011.0770142
Produced by FastQC (version 0.11.5)