##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295029_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3038228 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.411381897606105 33.0 33.0 33.0 22.0 33.0 2 30.238103921101377 33.0 33.0 33.0 22.0 33.0 3 30.471107171680334 33.0 33.0 33.0 22.0 33.0 4 33.55402984897776 37.0 33.0 37.0 22.0 37.0 5 34.37404796480053 37.0 33.0 37.0 27.0 37.0 6 34.02310129457039 37.0 37.0 37.0 27.0 37.0 7 34.128800406026144 37.0 37.0 37.0 27.0 37.0 8 33.924442471071956 37.0 37.0 37.0 27.0 37.0 9 33.788106751698685 37.0 37.0 37.0 22.0 37.0 10 33.90824289684645 37.0 37.0 37.0 27.0 37.0 11 34.10810149863671 37.0 37.0 37.0 27.0 37.0 12 34.08851211956443 37.0 37.0 37.0 27.0 37.0 13 33.77040630262113 37.0 37.0 37.0 27.0 37.0 14 35.23580850416756 37.0 33.0 40.0 27.0 40.0 15 35.209333203433054 37.0 33.0 40.0 27.0 40.0 16 35.20354923988588 37.0 33.0 40.0 27.0 40.0 17 35.300581786488706 37.0 37.0 40.0 27.0 40.0 18 35.16556064916787 37.0 33.0 40.0 27.0 40.0 19 35.04232763308086 37.0 33.0 40.0 27.0 40.0 20 35.2050915862799 37.0 33.0 40.0 27.0 40.0 21 35.16042443160948 37.0 33.0 40.0 27.0 40.0 22 35.23265205902914 37.0 33.0 40.0 27.0 40.0 23 35.2227627419667 37.0 33.0 40.0 27.0 40.0 24 35.35260256965574 37.0 33.0 40.0 27.0 40.0 25 34.201669854928596 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5236912736818233 1101 2 0.4296311395262187 1101 3 0.32515877183376674 1101 4 0.5176469973352766 1101 5 0.25261186289557 1101 6 0.33246534957751095 1101 7 0.26844239099041545 1101 8 0.3450120157231922 1101 9 0.3369303853884489 1101 10 0.5011475888832422 1101 11 0.3244987895462117 1101 12 0.386899412820334 1101 13 0.3898237282520327 1101 14 0.933048325212539 1101 15 0.7520476239350202 1101 16 0.8353374734693944 1101 17 0.7254120884816615 1101 18 0.8144281757707361 1101 19 0.8049625200564421 1101 20 0.67137625567198 1101 21 0.7043289325048505 1101 22 0.7527839242669501 1101 23 0.8252571923209047 1101 24 0.8277706465944945 1101 25 0.9515412268914929 1102 1 0.5099961481298934 1102 2 0.45539159771360005 1102 3 0.47565930615084895 1102 4 0.6798281161465951 1102 5 0.3166908558462751 1102 6 0.4361224438320477 1102 7 0.5330322040717093 1102 8 0.48023528602892185 1102 9 0.5301961028664053 1102 10 0.7099952653169268 1102 11 0.5740167927604958 1102 12 0.560356551279753 1102 13 0.5465798930297154 1102 14 1.005531698053467 1102 15 0.8943470830752815 1102 16 0.833780761205432 1102 17 0.8172114536760589 1102 18 0.8991180097382028 1102 19 0.7921893955345567 1102 20 0.8410002247839188 1102 21 0.7389358718326022 1102 22 0.7528905570124493 1102 23 0.8139012243262442 1102 24 0.936994347625216 1102 25 1.0196067851647683 1103 1 0.3532081179571911 1103 2 0.33486870351655185 1103 3 0.22393117887631675 1103 4 0.3580014499486168 1103 5 0.11498676623799042 1103 6 0.10451678975939416 1103 7 0.06574503822111666 1103 8 0.22353094828989128 1103 9 0.25432413006153354 1103 10 0.4312240397991616 1103 11 0.26919964444447686 1103 12 0.3176596078370224 1103 13 0.2291025452992841 1103 14 0.6106871829836535 1103 15 0.620759774135955 1103 16 0.49035426166860674 1103 17 0.5656771785486825 1103 18 0.5014607258600847 1103 19 0.5732813672641939 1103 20 0.5237031337033926 1103 21 0.49078139596745274 1103 22 0.5066808575814932 1103 23 0.6456941654200037 1103 24 0.5862221440587518 1103 25 0.6875496365179075 1104 1 0.4139942892958146 1104 2 0.39862629858632914 1104 3 0.3481971337538745 1104 4 0.3706915882443056 1104 5 0.33750891174972253 1104 6 0.18659456791252182 1104 7 0.2795606913630806 1104 8 0.3510208782521431 1104 9 0.356209315749048 1104 10 0.5723184659721312 1104 11 0.31509113458822213 1104 12 0.44259589017421064 1104 13 0.2970834302525418 1104 14 0.6771138863691775 1104 15 0.5460233295487669 1104 16 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3 0.23942370214470543 2212 4 0.19819382894833382 2212 5 0.20497177849927084 2212 6 0.12841989605393422 2212 7 0.24151351037539115 2212 8 0.28536643215080204 2212 9 0.19699649684670817 2212 10 0.3321768985311522 2212 11 0.22941296265504718 2212 12 0.21439377645376112 2212 13 0.1655931709516878 2212 14 0.18706388935796525 2212 15 0.17411674717360626 2212 16 0.101500963898701 2212 17 0.11295710650782809 2212 18 0.23192198053011737 2212 19 0.21889831057564635 2212 20 0.191931308782614 2212 21 0.3473334682326552 2212 22 -0.26496240307740493 2212 23 0.24582223112923884 2212 24 0.3357031819207066 2212 25 0.3090191827415225 2213 1 -0.22813475237845893 2213 2 -0.8458860892871272 2213 3 -0.2371958590633838 2213 4 -0.1620410968163668 2213 5 -0.01750769625975579 2213 6 -0.06087056633497667 2213 7 -0.0025240323117330377 2213 8 0.028991073065959938 2213 9 -0.10203201986416843 2213 10 -1.4927354218653548 2213 11 -0.42937032597588143 2213 12 -0.07441616567739828 2213 13 -0.1061780272680295 2213 14 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-1.218368201216613 2214 25 -1.6723185408684955 2215 1 -0.3870868945733541 2215 2 -0.13940432460789864 2215 3 -1.2512292877254367 2215 4 -0.86845631002506 2215 5 -0.24882862640314585 2215 6 -0.2108190896983615 2215 7 -0.23124629636008365 2215 8 -0.013119015919130561 2215 9 -0.17497853121648177 2215 10 -1.446556652634051 2215 11 -0.5012250010914272 2215 12 -0.4471988362408652 2215 13 -0.5236121598289785 2215 14 -0.8005163572852751 2215 15 -0.9104062411793237 2215 16 -0.6665928195509778 2215 17 -0.4090364565552278 2215 18 -0.3666568463999056 2215 19 -1.142212496398102 2215 20 -1.272554745128943 2215 21 -0.511986143465613 2215 22 -0.32775851338646333 2215 23 -0.67020014225794 2215 24 -1.805816104124652 2215 25 -0.7016940993194609 2216 1 -0.4247425215618783 2216 2 -0.35338143642950115 2216 3 -1.2819956555280854 2216 4 -0.6216969014921645 2216 5 -0.5585828475320653 2216 6 -0.43522079948597536 2216 7 -0.3343264956405392 2216 8 -0.3720834757302498 2216 9 -0.30128233634559365 2216 10 -0.21080908799778086 2216 11 -0.342675611506003 2216 12 -0.1635406372405228 2216 13 -1.0853380884737973 2216 14 -0.6387126627317699 2216 15 -1.139899776534044 2216 16 -0.8224167548423793 2216 17 -1.806758581186429 2216 18 -0.8682070120508527 2216 19 -0.6215988823000913 2216 20 -0.4644637264362643 2216 21 -0.31827271685922653 2216 22 -0.42181935458624764 2216 23 -0.21587088118811693 2216 24 -0.45195090651124303 2216 25 -0.4676695311707988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3692.0 3 409.0 4 490.0 5 359.0 6 265.0 7 240.0 8 440.0 9 899.0 10 1602.0 11 2784.0 12 5020.0 13 8560.0 14 14905.0 15 24369.0 16 32221.0 17 33992.0 18 32057.0 19 30552.0 20 29992.0 21 30370.0 22 30310.0 23 29134.0 24 28511.0 25 28877.0 26 28271.0 27 29660.0 28 31611.0 29 35732.0 30 42567.0 31 53771.0 32 71873.0 33 102203.0 34 149997.0 35 240938.0 36 450084.0 37 1431471.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.785042410640614 18.087857685072116 10.481534044027855 23.645565860259417 2 10.302842084892315 26.296612424177386 33.06945629923999 30.331089191690314 3 23.28808950765973 33.61097564232812 24.38484257648106 18.716092273531093 4 13.255507077217976 23.78550111096343 26.669322732815026 36.289669079003566 5 9.538785035321503 44.29515013040618 33.55305219084366 12.613012643428656 6 32.14163712238718 29.007866164413915 19.738645236512507 19.111851476686397 7 21.154104864428554 36.95644145724468 22.020039620257506 19.869414058069264 8 29.45724418636096 34.77320261481018 21.440830059104314 14.32872313972455 9 28.544696563203182 20.204319419513077 17.58448367578399 33.666500341499756 10 17.828641009970617 35.503324693297415 28.783423795905623 17.88461050082635 11 24.413081292301097 31.825824588015134 15.084870118628558 28.67622400105521 12 25.023660798788566 37.0681121023914 24.739038702141706 13.169188396678322 13 30.19183480162845 23.54812920997016 18.903870863906324 27.356165124495067 14 21.149947303370343 33.291409064347924 22.57882400117133 22.979819631110402 15 38.472125772177414 21.667626220958645 18.93764479872112 20.922603208142824 16 15.333933035988096 29.70361542880169 35.30657050320285 19.655881032007372 17 28.20637279649305 32.450672012118936 25.229068435792417 14.113886755595594 18 28.73652709420523 24.57866923882307 28.220084254610505 18.46471941236119 19 22.794607187668483 35.312599441724885 29.291241214091745 12.601552156514886 20 24.671054150181725 28.62585678267184 25.39420411625007 21.30888495089637 21 17.25762802294136 32.52270905404305 30.962464234497055 19.257198688518535 22 21.317264012071778 31.701999124163077 16.736861127086048 30.243875736679097 23 14.385601932722093 33.309216078184754 26.42910400879565 25.876077980297502 24 20.36869323166428 42.21351160597086 19.456804944493182 17.96099021787168 25 21.195270760503927 25.90076512103423 17.380054086142074 35.52391003231977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23151.0 1 23151.0 2 24485.0 3 25819.0 4 25819.0 5 25819.0 6 31348.5 7 36878.0 8 36878.0 9 36878.0 10 35546.5 11 34215.0 12 34215.0 13 34215.0 14 34152.0 15 34089.0 16 34089.0 17 34089.0 18 37371.5 19 40654.0 20 40654.0 21 40654.0 22 46941.0 23 53228.0 24 53228.0 25 53228.0 26 61378.0 27 69528.0 28 69528.0 29 69528.0 30 77656.5 31 85785.0 32 85785.0 33 85785.0 34 91817.5 35 97850.0 36 97850.0 37 97850.0 38 107740.5 39 117631.0 40 117631.0 41 117631.0 42 170085.0 43 222539.0 44 222539.0 45 222539.0 46 669417.5 47 1116296.0 48 1116296.0 49 1116296.0 50 922480.5 51 728665.0 52 728665.0 53 728665.0 54 472701.0 55 216737.0 56 216737.0 57 216737.0 58 139156.0 59 61575.0 60 61575.0 61 61575.0 62 49071.0 63 36567.0 64 36567.0 65 36567.0 66 28090.5 67 19614.0 68 19614.0 69 19614.0 70 14740.0 71 9866.0 72 9866.0 73 9866.0 74 7062.0 75 4258.0 76 4258.0 77 4258.0 78 3114.5 79 1971.0 80 1971.0 81 1971.0 82 1407.5 83 844.0 84 844.0 85 844.0 86 583.5 87 323.0 88 323.0 89 323.0 90 211.5 91 100.0 92 100.0 93 100.0 94 65.0 95 30.0 96 30.0 97 30.0 98 22.5 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2741071440326401 2 0.16447086920402287 3 0.1826393542551777 4 0.20324346954869746 5 0.22809348080525885 6 0.18076326069011278 7 0.17892008104724202 8 0.19623280412134966 9 0.19797724199763808 10 0.20469168212523878 11 0.18606240216336628 12 0.1904399538151844 13 0.2020914822719032 14 0.19011081459324317 15 0.18333054662125423 16 0.18477875919779557 17 0.18494332880876616 18 0.18935379438277838 19 0.1867206806072487 20 0.18635862746311338 21 0.18520664018631913 22 0.18760935650648997 23 0.1915919410919786 24 0.2030130720933386 25 0.1984051229861617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3038228.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.327024301881877 #Duplication Level Percentage of deduplicated Percentage of total 1 74.58010732048427 14.41411546619958 2 11.78601169572582 4.55577068931114 3 4.98739225791689 2.8917435411533177 4 2.6970604709140655 2.085046130600045 5 1.5941318956714887 1.5404912944023943 6 1.0115506254884117 1.1730158112839006 7 0.6875013740514501 0.9301149034708691 8 0.4765495428911128 0.736822767720579 9 0.33263535026404584 0.5785966348396383 >10 1.603198641861527 5.425050752915011 >50 0.11299696141296098 1.521086482488391 >100 0.0975499694069389 3.8214825582753678 >500 0.011958833711663173 1.666668837693287 >1k 0.012471355156448738 5.315034889972933 >5k 0.002904288187118199 3.573546400037774 >10k+ 0.005979416855831587 49.77141283963577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 231875 7.6319157087618175 No Hit CCCATGTACTCTGCGTTGATACCAC 223781 7.365510422522601 No Hit GAGTACATGGGAAGCAGTGGTATCA 105629 3.476664687442812 No Hit CATGTACTCTGCGTTGATACCACTG 82687 2.721553484465287 No Hit GCGTTGATACCACTGCTTCCCATGT 72438 2.38421869589774 No Hit GTATCAACGCAGAGTACATGGGAAG 60014 1.9752961265579805 No Hit ACGCAGAGTACATGGGAAGCAGTGG 59381 1.9544616138091018 No Hit TATCAACGCAGAGTACATGGGAAGC 57987 1.9085796062704972 No Hit ACTCTGCGTTGATACCACTGCTTCC 53386 1.7571426502553462 No Hit GGTATCAACGCAGAGTACATGGGAA 49290 1.6223272249482266 No Hit GCTTCCCATGTACTCTGCGTTGATA 40561 1.3350215981157438 No Hit GTACTCTGCGTTGATACCACTGCTT 26930 0.8863719246876798 No Hit ATACCACTGCTTCCCATGTACTCTG 26854 0.8838704666009266 No Hit GTGGTATCAACGCAGAGTACATGGG 26803 0.8821918565690263 No Hit CAGTGGTATCAACGCAGAGTACATG 24890 0.8192275234116728 No Hit GGGAAGCAGTGGTATCAACGCAGAG 23435 0.7713377666192267 No Hit ACATGGGAAGCAGTGGTATCAACGC 22650 0.7455003376968418 No Hit CATGGGAAGCAGTGGTATCAACGCA 22309 0.7342766902286464 No Hit TACCACTGCTTCCCATGTACTCTGC 21878 0.7200907897629802 No Hit GCAGTGGTATCAACGCAGAGTACAT 21854 0.7193008556303213 No Hit CTGCTTCCCATGTACTCTGCGTTGA 21707 0.7144625090677856 No Hit GATACCACTGCTTCCCATGTACTCT 21247 0.6993221048584899 No Hit GTTGATACCACTGCTTCCCATGTAC 20456 0.6732871924029401 No Hit CCATGTACTCTGCGTTGATACCACT 19522 0.6425455890736311 No Hit GCAGAGTACATGGGAAGCAGTGGTA 19285 0.6347449895136243 No Hit CTTCCCATGTACTCTGCGTTGATAC 18456 0.607459348014698 No Hit CCACTGCTTCCCATGTACTCTGCGT 18007 0.5926809969495377 No Hit AAGCAGTGGTATCAACGCAGAGTAC 16403 0.5398870657501675 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 15695 0.5165840088367298 No Hit TTCCCATGTACTCTGCGTTGATACC 15478 0.5094416877206055 No Hit ATGGGAAGCAGTGGTATCAACGCAG 15226 0.501147379327687 No Hit GTACATGGTAAGCAGTGGTATCAAC 14866 0.48929836733780346 No Hit TGATACCACTGCTTCCCATGTACTC 13324 0.4385450993144688 No Hit ACCATGTACTCTGCGTTGATACCAC 11745 0.3865740161699517 No Hit ATCAACGCAGAGTACATGGGAAGCA 11336 0.3731122219925562 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 9446 0.3109049090456674 No Hit CCCCATGTACTCTGCGTTGATACCA 8526 0.28062410062707605 No Hit TCCATGTACTCTGCGTTGATACCAC 7756 0.2552803805376028 No Hit GGAAGCAGTGGTATCAACGCAGAGT 6996 0.23026579967007085 No Hit GTATCAACGCAGAGTACTTTTTTTT 6929 0.22806056688306472 No Hit GGTAAGCAGTGGTATCAACGCAGAG 6645 0.21871301297993434 No Hit AAAAAGTACTCTGCGTTGATACCAC 6490 0.21361135503984557 No Hit GTACATGGGTAAGCAGTGGTATCAA 6433 0.21173526147478067 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 6065 0.19962293810734413 No Hit CTGCGTTGATACCACTGCTTCCCAT 5753 0.18935379438277838 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5555 0.18283683778834242 No Hit CTCTGCGTTGATACCACTGCTTCCC 5523 0.18178359227813054 No Hit GAGTACATGGTAAGCAGTGGTATCA 5361 0.17645153688268292 No Hit GTACATGGAAAGCAGTGGTATCAAC 5350 0.1760894837385476 No Hit TCCCATGTACTCTGCGTTGATACCA 5288 0.17404882056251209 No Hit TCAACGCAGAGTACATGGGAAGCAG 5096 0.16772934750124086 No Hit GGTATCAACGCAGAGTACTTTTTTT 5017 0.1651291476479053 No Hit TGGGAAGCAGTGGTATCAACGCAGA 4997 0.16447086920402287 No Hit TATCAACGCAGAGTACTTTTTTTTT 4904 0.16140987443996963 No Hit GCTTACCATGTACTCTGCGTTGATA 4824 0.15877676066443994 No Hit AAAGTACTCTGCGTTGATACCACTG 4729 0.15564993805599844 No Hit AGTGGTATCAACGCAGAGTACATGG 4154 0.13672443279437882 No Hit TTGATACCACTGCTTCCCATGTACT 3956 0.13020747619994286 No Hit GCGTTGATACCACTGCTTACCATGT 3908 0.12862760793462505 No Hit ACTCTGCGTTGATACCACTGCTTAC 3849 0.1266856865251719 No Hit GAGTACATGGAAAGCAGTGGTATCA 3711 0.12214356526238321 No Hit AACGCAGAGTACATGGGAAGCAGTG 3493 0.11496833022406482 No Hit ACTGCTTCCCATGTACTCTGCGTTG 3339 0.10989958620617017 No Hit GTACATGGAAGCAGTGGTATCAACG 3288 0.10822097617427 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 3276 0.10782600910794055 No Hit ACGCAGAGTACATGGTAAGCAGTGG 3244 0.10677276359772868 No Hit CTGCTTACCATGTACTCTGCGTTGA 3237 0.10654236614236984 No Hit GTATCAACGCAGAGTACATGGTAAG 3085 0.10153944996886342 No Hit TATCAACGCAGAGTACATGGTAAGC 3082 0.10144070820228106 No Hit ATGTACTCTGCGTTGATACCACTGC 3068 0.10097991329156339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.291392219412105E-5 0.0 8 0.0 0.0 0.0 3.291392219412105E-5 0.0 9 0.0 0.0 0.0 3.291392219412105E-5 0.0 10 0.0 0.0 0.0 1.316556887764842E-4 0.0 11 0.0 0.0 0.0 1.9748353316472627E-4 0.0 12 0.0 0.0 0.0 2.3039745535884733E-4 0.0 13 0.0 3.291392219412105E-5 0.0 2.3039745535884733E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGA 35 0.0021628237 16.294737 4 GTACAAA 780 0.0 15.119913 1 GTACATA 1005 0.0 14.384664 1 TACATAA 740 0.0 13.486531 2 AGAACAA 1595 0.0 13.0504675 2 GGACCGT 75 1.4820469E-5 12.6647 6 AGGCTAG 70 1.0943231E-4 12.2109785 18 TACATGA 695 0.0 11.898086 2 ATGCGTT 65 8.043314E-4 11.6895275 11 CGCAAAA 735 0.0 11.638522 2 TAGAACC 100 1.9185572E-6 11.406315 4 AGGACTC 75 2.0650416E-4 11.40594 5 GTACAAG 410 0.0 11.3667 1 ACATAAA 1535 0.0 11.331447 3 GAGAACA 2420 0.0 11.161566 1 CGCAGAA 470 0.0 11.122629 2 GGTTTAG 60 0.005827831 11.096065 1 CTATTCC 120 1.2669989E-7 11.089474 4 CCTACGC 60 0.0058558197 11.088924 3 AAAAGTA 1330 0.0 11.077014 2 >>END_MODULE