FastQCFastQC Report
Wed 25 May 2016
SRR1295029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038228
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2504638.24371969450614No Hit
GTACATGGGAAGCAGTGGTATCAAC2237647.364950885845302No Hit
GAGTACATGGGAAGCAGTGGTATCA1118923.682804582144592No Hit
CATGTACTCTGCGTTGATACCACTG925433.0459531016105443No Hit
GCGTTGATACCACTGCTTCCCATGT789342.5980275344707504No Hit
ACGCAGAGTACATGGGAAGCAGTGG720812.3724684256744393No Hit
TATCAACGCAGAGTACATGGGAAGC625272.058008813031807No Hit
GTATCAACGCAGAGTACATGGGAAG622252.048068808529182No Hit
ACTCTGCGTTGATACCACTGCTTCC592811.9511702215896896No Hit
GGTATCAACGCAGAGTACATGGGAA529111.741508537213139No Hit
GCTTCCCATGTACTCTGCGTTGATA442581.4567043684674092No Hit
CAGTGGTATCAACGCAGAGTACATG302860.99683104757115No Hit
GTGGTATCAACGCAGAGTACATGGG287280.9455511567927094No Hit
ACATGGGAAGCAGTGGTATCAACGC285310.9390671141204676No Hit
ATACCACTGCTTCCCATGTACTCTG279060.918495912749142No Hit
CATGGGAAGCAGTGGTATCAACGCA274450.9033225946176522No Hit
GGGAAGCAGTGGTATCAACGCAGAG263290.866590657449013No Hit
GTACTCTGCGTTGATACCACTGCTT261510.8607319792984595No Hit
GCAGTGGTATCAACGCAGAGTACAT255040.8394366716388632No Hit
CTGCTTCCCATGTACTCTGCGTTGA238000.7833513482200809No Hit
GATACCACTGCTTCCCATGTACTCT234640.7722922703628563No Hit
CCATGTACTCTGCGTTGATACCACT229400.7550453751331369No Hit
TACCACTGCTTCCCATGTACTCTGC229010.753761732167566No Hit
GTATCAACGCAGAGTACTTTTTTTT227970.7503386842593776No Hit
GCAGAGTACATGGGAAGCAGTGGTA218070.7177539012871977No Hit
CTTCCCATGTACTCTGCGTTGATAC209300.6888883915229534No Hit
CCACTGCTTCCCATGTACTCTGCGT204030.6715427545266517No Hit
GTTGATACCACTGCTTCCCATGTAC202400.6661777852090099No Hit
AAGCAGTGGTATCAACGCAGAGTAC189020.622138957313276No Hit
GGTATCAACGCAGAGTACTTTTTTT183460.6038388165733447No Hit
TATCAACGCAGAGTACTTTTTTTTT178410.5872172858653135No Hit
ATGGGAAGCAGTGGTATCAACGCAG177360.583761324034931No Hit
GTACTTTTTTTTTTTTTTTTTTTTT177280.5834980126573779No Hit
GTACATGGTAAGCAGTGGTATCAAC152760.5027930754373932No Hit
TTCCCATGTACTCTGCGTTGATACC150190.49433419743350404No Hit
ACCATGTACTCTGCGTTGATACCAC130860.43071158583226804No Hit
TGATACCACTGCTTCCCATGTACTC130550.42969125424425025No Hit
ATCAACGCAGAGTACATGGGAAGCA130270.4287696644228149No Hit
ACGCAGAGTACTTTTTTTTTTTTTT117590.3870348110806694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA113040.3720589764823443No Hit
CCCCATGTACTCTGCGTTGATACCA99620.3278884928978339No Hit
GAGTACTTTTTTTTTTTTTTTTTTT87690.28862218372024745No Hit
GGAAGCAGTGGTATCAACGCAGAGT82660.27206648085660456No Hit
TCCATGTACTCTGCGTTGATACCAC78450.25820971961287964No Hit
GGTAAGCAGTGGTATCAACGCAGAG77240.25422713502739097No Hit
GTACATGGGTAAGCAGTGGTATCAA71800.23632196135378913No Hit
CTGCGTTGATACCACTGCTTCCCAT65670.21614572704879292No Hit
AAAAAGTACTCTGCGTTGATACCAC65580.21584950174904582No Hit
CTCTGCGTTGATACCACTGCTTCCC60140.19794432807544396No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA59300.1951795586111378No Hit
TCAACGCAGAGTACATGGGAAGCAG58490.192513530913414No Hit
GAGTACATGGTAAGCAGTGGTATCA58160.191427371481008No Hit
TGGGAAGCAGTGGTATCAACGCAGA57180.18820180710598414No Hit
TCCCATGTACTCTGCGTTGATACCA56140.18477875919779557No Hit
GTACATGGAAAGCAGTGGTATCAAC52880.17404882056251209No Hit
GCTTACCATGTACTCTGCGTTGATA52460.172666435830359No Hit
AAAGTACTCTGCGTTGATACCACTG49150.16177192758410494No Hit
AGTGGTATCAACGCAGAGTACATGG45900.1510749028710156No Hit
AACGCAGAGTACATGGGAAGCAGTG43990.14478834373193847No Hit
ACTCTGCGTTGATACCACTGCTTAC43460.14304390585565008No Hit
GCGTTGATACCACTGCTTACCATGT42120.13863344028163785No Hit
GAGTACATGGAAAGCAGTGGTATCA40190.1322810532981725No Hit
ACGCAGAGTACATGGTAAGCAGTGG39740.13079992679943703No Hit
ACTGCTTCCCATGTACTCTGCGTTG37860.12461210942694227No Hit
TTGATACCACTGCTTCCCATGTACT37160.12230813487335382No Hit
GCAGAGTACTTTTTTTTTTTTTTTT36780.12105740582997722No Hit
CAACGCAGAGTACATGGGAAGCAGT36130.11891800088735935No Hit
CTGCTTACCATGTACTCTGCGTTGA35220.11592283396769433No Hit
GTGGTATCAACGCAGAGTACTTTTT34790.11450753531334712No Hit
CGCAGAGTACATGGGAAGCAGTGGT34220.11263144174828221No Hit
GTACATGGAAGCAGTGGTATCAACG33010.10864885716279357No Hit
CGTTGATACCACTGCTTCCCATGTA32770.10785892303013468No Hit
GCTTTCCATGTACTCTGCGTTGATA32320.10637779653139923No Hit
TATCAACGCAGAGTACATGGTAAGC32310.10634488260920509No Hit
ATGGTAAGCAGTGGTATCAACGCAG31190.10265852332346354No Hit
GTATCAACGCAGAGTACATGGTAAG30740.10117739682472811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC804.3291948E-1016.6238219
GTACAAA5550.016.4523851
GGGACGC1850.015.9177887
GCGCTCC551.1297761E-515.54435119
GGACGCA1850.015.4043118
ACTGGTC456.768605E-414.7767288
TATGCGT653.3771757E-614.614346510
TATACTG801.2906457E-714.2492235
TGCGCTC602.5715752E-514.24898818
TGGGACG2150.014.1385326
CGCAAAA3150.013.8722592
GATACGA1001.0095391E-813.29905718
CCAGATC655.4586264E-513.1531293
GCCTTAA604.0523298E-412.6820471
ACGCAGT2650.012.54627910
ACGCCGA1301.9826984E-1012.42219457
ACGCAAA5300.012.3829411
GTCCTGG853.8936323E-612.3090451
GAACAAA10250.012.2490011
CTATGCG701.0922861E-412.2134189
Produced by FastQC (version 0.11.5)