FastQCFastQC Report
Wed 25 May 2016
SRR1295028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5549953
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC854741.5400851142343008No Hit
CCCATGTACTCTGCGTTGATACCAC796101.4344265618105234No Hit
GAGTACATGGGAAGCAGTGGTATCA396000.7135195559313746No Hit
CATGTACTCTGCGTTGATACCACTG313220.5643651396687503No Hit
GCGTTGATACCACTGCTTCCCATGT254770.45904893248645523No Hit
GTATCAACGCAGAGTACATGGGAAG219490.3954808265943873No Hit
ACGCAGAGTACATGGGAAGCAGTGG210270.3788680732971973No Hit
ATTCCATTCCATTCCATTCCATTCC200800.36180486573489906No Hit
GCTTCCCATGTACTCTGCGTTGATA192170.3462551845033643No Hit
TATCAACGCAGAGTACATGGGAAGC191470.3449939125610613No Hit
ACTCTGCGTTGATACCACTGCTTCC179900.32414688917185425No Hit
GGTATCAACGCAGAGTACATGGGAA174700.31477744045760386No Hit
GAATGGAATGGAATGGAATGGAATG149090.26863290553992075No Hit
GTGGTATCAACGCAGAGTACATGGG106020.19102864474708164No Hit
CAGTGGTATCAACGCAGAGTACATG95330.1717672203710554No Hit
ATACCACTGCTTCCCATGTACTCTG87100.15693826596369376No Hit
GCAGTGGTATCAACGCAGAGTACAT83720.150848124299431No Hit
GGGAAGCAGTGGTATCAACGCAGAG80330.14473996446456394No Hit
ACATGGGAAGCAGTGGTATCAACGC79820.14382103776374322No Hit
GTACATGGTAAGCAGTGGTATCAAC79290.14286607472171386No Hit
GTACTCTGCGTTGATACCACTGCTT79030.14239760228600135No Hit
CATGGGAAGCAGTGGTATCAACGCA75820.13661376952201215No Hit
CTGCTTCCCATGTACTCTGCGTTGA73290.1320551723591173No Hit
GATACCACTGCTTCCCATGTACTCT70940.1278209022671003No Hit
TACCACTGCTTCCCATGTACTCTGC70860.12767675690226565No Hit
CCATGTACTCTGCGTTGATACCACT70120.12634341227754542No Hit
GTATCAACGCAGAGTACTTTTTTTT69270.12481186777617756No Hit
GTTGATACCACTGCTTCCCATGTAC67700.12198301499129813No Hit
CTTCCCATGTACTCTGCGTTGATAC65330.11771270855807248No Hit
AAGCAGTGGTATCAACGCAGAGTAC62140.11196491213529196No Hit
GCAGAGTACATGGGAAGCAGTGGTA61210.11028922226908949No Hit
ACCATGTACTCTGCGTTGATACCAC59710.10758649667844034No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN59290.10682973351305858No Hit
CCACTGCTTCCCATGTACTCTGCGT58350.10513602547625178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT604.096743E-412.6660179
ACCGTTA701.0923059E-412.2137698
AAACCGT752.0789001E-411.3980776
TAAGCCG600.005865730311.0866695
CTAGGAC4000.010.4528613
TATTCCG5100.010.2481815
AGGACCG1502.4381734E-810.1363835
GGACCGA856.608873E-410.0571276
TCGGAAC1951.6370905E-109.741858513
GTCCTAG4700.09.50818351
TTACGCT900.00111084919.5033744
CTAGACT4400.09.5033734
TAGACAG6150.09.42565
GTATTAG6400.09.2110531
CGCAAAA2600.09.1371162
TTCTACG1252.748118E-59.1224982
GGAACGA5750.09.08525915
GCTAGAC2651.8189894E-128.9647193
TAACGCT850.0074181428.9443524
CCGTCCA850.0074429128.9407189
Produced by FastQC (version 0.11.5)