FastQCFastQC Report
Wed 25 May 2016
SRR1295028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5549953
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC891111.6056172007222405No Hit
GTACATGGGAAGCAGTGGTATCAAC816401.4710034481373087No Hit
GAGTACATGGGAAGCAGTGGTATCA411900.7421684471922555No Hit
CATGTACTCTGCGTTGATACCACTG341120.6146358356548244No Hit
GCGTTGATACCACTGCTTCCCATGT277940.5007970337766825No Hit
ACGCAGAGTACATGGGAAGCAGTGG250080.45059841047302557No Hit
GTATCAACGCAGAGTACATGGGAAG221880.3997871693688217No Hit
GCTTCCCATGTACTCTGCGTTGATA207350.3736067674807336No Hit
TATCAACGCAGAGTACATGGGAAGC201210.36254361072967645No Hit
ACTCTGCGTTGATACCACTGCTTCC197420.3557147240706363No Hit
ATTCCATTCCATTCCATTCCATTCC194280.3500570185008774No Hit
GGTATCAACGCAGAGTACATGGGAA185000.33333615618006135No Hit
GTATCAACGCAGAGTACTTTTTTTT177670.32012883712708917No Hit
GAATGGAATGGAATGGAATGGAATG159290.287011439556335No Hit
GGTATCAACGCAGAGTACTTTTTTT149280.268975250781403No Hit
TATCAACGCAGAGTACTTTTTTTTT127440.22962356618155144No Hit
CAGTGGTATCAACGCAGAGTACATG110180.19852420371848195No Hit
GTGGTATCAACGCAGAGTACATGGG108710.19587553263964574No Hit
ACATGGGAAGCAGTGGTATCAACGC94590.17043387574633515No Hit
CATGGGAAGCAGTGGTATCAACGCA94480.17023567586968755No Hit
GCAGTGGTATCAACGCAGAGTACAT94100.1695509853867231No Hit
ATACCACTGCTTCCCATGTACTCTG89860.1619112810504882No Hit
GGGAAGCAGTGGTATCAACGCAGAG89370.16102839069087613No Hit
CCATGTACTCTGCGTTGATACCACT82690.14899225272718525No Hit
CTGCTTCCCATGTACTCTGCGTTGA79980.1441093284934125No Hit
GTACTCTGCGTTGATACCACTGCTT79780.1437489650813259No Hit
ACGCAGAGTACTTTTTTTTTTTTTT78500.14144263924397196No Hit
GTACATGGTAAGCAGTGGTATCAAC77660.13992911291320845No Hit
GATACCACTGCTTCCCATGTACTCT77590.13980298571897817No Hit
CTTCCCATGTACTCTGCGTTGATAC73880.1331182444247726No Hit
TACCACTGCTTCCCATGTACTCTGC73000.13153264541159176No Hit
GCAGAGTACATGGGAAGCAGTGGTA68750.12387492290475252No Hit
AAGCAGTGGTATCAACGCAGAGTAC68080.12266770547426258No Hit
CCACTGCTTCCCATGTACTCTGCGT66920.12057759768416057No Hit
GTTGATACCACTGCTTCCCATGTAC66020.11895596232977107No Hit
ACCATGTACTCTGCGTTGATACCAC65040.11719018161054698No Hit
ATGGGAAGCAGTGGTATCAACGCAG59410.1070459515603105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC657.9508004E-411.7059791
GGAACGA5650.011.09660815
TACGACC600.00588179211.0825984
AACCGTG1502.1409505E-910.7659537
CCTACAC5350.010.1208243
TCGGCGG856.602664E-410.05815618
CGGGACT951.6466553E-49.9993364
ACGAATT1901.0004442E-109.99933618
CTCGGAA3750.09.87934412
GAACGAT2650.09.67860216
TAGAACG1002.759545E-49.499374
GGTCCGC1201.7036793E-59.4993711
ATCGGCG1106.84818E-59.4993717
AGGTCCG1106.84818E-59.4993710
TAGACAG7200.09.3674345
TATTCCG5250.09.2279595
TCGGAAC3100.09.19293813
AGGACCG1451.7101702E-69.1718055
AACGATT2800.09.16010617
CTATGCG1151.1124625E-49.0863539
Produced by FastQC (version 0.11.5)