FastQCFastQC Report
Wed 25 May 2016
SRR1295027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4712645
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC701621.4888029970430618No Hit
CCCATGTACTCTGCGTTGATACCAC650081.3794376618650461No Hit
GAGTACATGGGAAGCAGTGGTATCA332660.7058880946899246No Hit
CATGTACTCTGCGTTGATACCACTG253750.5384449709239716No Hit
GCGTTGATACCACTGCTTCCCATGT213420.4528667022447055No Hit
GCTTCCCATGTACTCTGCGTTGATA188560.4001150097238388No Hit
GTATCAACGCAGAGTACATGGGAAG184360.3912028171016489No Hit
ACGCAGAGTACATGGGAAGCAGTGG182570.38740452548409654No Hit
TATCAACGCAGAGTACATGGGAAGC160220.3399789290303004No Hit
ACTCTGCGTTGATACCACTGCTTCC148190.31445186301959943No Hit
GGTATCAACGCAGAGTACATGGGAA146500.3108657664644801No Hit
GTGGTATCAACGCAGAGTACATGGG90020.19101799520226964No Hit
CAGTGGTATCAACGCAGAGTACATG82730.1755489751508972No Hit
ATACCACTGCTTCCCATGTACTCTG72220.1532472740891792No Hit
ACATGGGAAGCAGTGGTATCAACGC71950.15267434742060987No Hit
GCAGTGGTATCAACGCAGAGTACAT68820.14603264196645407No Hit
CATGGGAAGCAGTGGTATCAACGCA68570.14550215431037136No Hit
GGGAAGCAGTGGTATCAACGCAGAG68490.14533239826042488No Hit
GTACTCTGCGTTGATACCACTGCTT65960.13996386318086765No Hit
GTACATGGTAAGCAGTGGTATCAAC62590.13281288957687243No Hit
CTGCTTCCCATGTACTCTGCGTTGA61840.13122142660862424No Hit
GTATCAACGCAGAGTACTTTTTTTT61800.131136548583651No Hit
CCATGTACTCTGCGTTGATACCACT59810.1269138668412325No Hit
GATACCACTGCTTCCCATGTACTCT58760.12468581868568501No Hit
CTTCCCATGTACTCTGCGTTGATAC56950.12084508805564602No Hit
TACCACTGCTTCCCATGTACTCTGC55750.11829874730644892No Hit
GTTGATACCACTGCTTCCCATGTAC55160.11704679643809368No Hit
AAGCAGTGGTATCAACGCAGAGTAC50910.10802850628468726No Hit
GGTATCAACGCAGAGTACTTTTTTT50520.1072009455411982No Hit
ATTCCATTCCATTCCATTCCATTCC50140.10639460430395245No Hit
GCAGAGTACATGGGAAGCAGTGGTA50100.10630972627897921No Hit
CCACTGCTTCCCATGTACTCTGCGT49660.1053760680042736No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN49310.1046333852857578No Hit
TTCCCATGTACTCTGCGTTGATACC47280.10032582551836601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1054.085604E-59.9575585
ACACCGT2002.6557245E-109.4991816
GTCTTAG4000.09.2708691
CATTTCG950.00182932578.99759915
TTAGACT3600.08.9774634
TAGGACC2900.08.8499944
GTGCTAG5050.08.8495731
TTATACT6800.08.8066114
CTTAGAC3350.08.7952443
GTTCTAG4800.08.7162031
GTACTGG5900.08.7027721
GTACAAA10000.08.6528111
CAGGACT4950.08.6414084
ACCGTCC1000.00290893558.5492638
GTACTAG3450.08.5439451
CTAGGAC4150.08.4739263
GTATTAG5250.08.3313311
CGTGTGA1606.4686556E-68.31134110
CTGTCGC3100.08.2733939
CCTATAC4750.08.2039173
Produced by FastQC (version 0.11.5)