FastQCFastQC Report
Wed 25 May 2016
SRR1295027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4712645
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC719471.5266798156873687No Hit
GTACATGGGAAGCAGTGGTATCAAC691301.4669044665999666No Hit
GAGTACATGGGAAGCAGTGGTATCA359030.7618439326535311No Hit
CATGTACTCTGCGTTGATACCACTG280610.5954405646935002No Hit
GCGTTGATACCACTGCTTCCCATGT229020.48596913198426783No Hit
ACGCAGAGTACATGGGAAGCAGTGG217760.46207596795430167No Hit
GCTTCCCATGTACTCTGCGTTGATA207540.4403896325736396No Hit
GTATCAACGCAGAGTACATGGGAAG193470.4105337872893035No Hit
TATCAACGCAGAGTACATGGGAAGC174060.3693467256710404No Hit
ACTCTGCGTTGATACCACTGCTTCC167590.3556177051316193No Hit
GTATCAACGCAGAGTACTTTTTTTT163220.3463447809032931No Hit
GGTATCAACGCAGAGTACATGGGAA155480.32992088307097184No Hit
GGTATCAACGCAGAGTACTTTTTTT138110.29306260072634366No Hit
TATCAACGCAGAGTACTTTTTTTTT111250.23606700695681512No Hit
CAGTGGTATCAACGCAGAGTACATG97950.20784506365321387No Hit
GTGGTATCAACGCAGAGTACATGGG96660.20510774734782697No Hit
ACATGGGAAGCAGTGGTATCAACGC89750.1904450685337003No Hit
CATGGGAAGCAGTGGTATCAACGCA84570.17945336429966613No Hit
GCAGTGGTATCAACGCAGAGTACAT80970.1718143420520748No Hit
GGGAAGCAGTGGTATCAACGCAGAG77380.16419653931072678No Hit
ATACCACTGCTTCCCATGTACTCTG75130.1594221504059822No Hit
CCATGTACTCTGCGTTGATACCACT70400.14938532395289694No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70120.14879117777808426No Hit
CTGCTTCCCATGTACTCTGCGTTGA66270.14062166787441022No Hit
GTACTCTGCGTTGATACCACTGCTT65390.13875435132499903No Hit
GATACCACTGCTTCCCATGTACTCT64440.13673849823188464No Hit
CTTCCCATGTACTCTGCGTTGATAC64100.13601703501961213No Hit
GTACATGGTAAGCAGTGGTATCAAC63940.13567752291971918No Hit
TACCACTGCTTCCCATGTACTCTGC59880.12706240338493563No Hit
GCAGAGTACATGGGAAGCAGTGGTA57500.12201216089902803No Hit
AAGCAGTGGTATCAACGCAGAGTAC57420.12184240484908156No Hit
CCACTGCTTCCCATGTACTCTGCGT54650.1159646016196849No Hit
GTTGATACCACTGCTTCCCATGTAC54590.11583728458222506No Hit
ATGGGAAGCAGTGGTATCAACGCAG54110.1148187482825462No Hit
ACCATGTACTCTGCGTTGATACCAC52340.11106289567748048No Hit
ATTCCATTCCATTCCATTCCATTCC47280.10032582551836601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG550.003071643212.0900656
CGACCAT700.001493245510.8563866
CGGTCTA700.001493245510.8563865
GAGGCGT803.7761743E-410.6867546
TAGGACC2500.010.2592834
GTGTAGG4850.010.1993571
AGAACCG750.002650814410.1326275
CGCAAAA1701.5643309E-910.0583342
TGCACCG856.6024804E-410.0581215
GGTGCGC1054.1121744E-59.9516878
CCGTGCA1304.247877E-69.4992369
ACGCAAA2750.09.33991
CTGCGTG1752.6777343E-89.227839
GCCTTAG3450.09.0992581
CGTTACA1054.4979426E-49.0471792
CAGTGCG850.00744434738.9404579
GTAGGAC3200.08.9056283
TACACCA8650.08.8953325
TACACTG11650.08.8877925
TCTAGAC3850.08.8824973
Produced by FastQC (version 0.11.5)