FastQCFastQC Report
Wed 25 May 2016
SRR1295026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182823
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC3038529.54661946328778No Hit
CCCATGTACTCTGCGTTGATACCAC2924969.189829280484652No Hit
GAGTACATGGGAAGCAGTGGTATCA1419804.460819844521671No Hit
CATGTACTCTGCGTTGATACCACTG1097173.4471599583137356No Hit
GCGTTGATACCACTGCTTCCCATGT912812.867925737623487No Hit
TATCAACGCAGAGTACATGGGAAGC768342.414020509465968No Hit
ACGCAGAGTACATGGGAAGCAGTGG767222.4105016207310306No Hit
GTATCAACGCAGAGTACATGGGAAG709902.2304099222608356No Hit
ACTCTGCGTTGATACCACTGCTTCC685642.154188278770136No Hit
GGTATCAACGCAGAGTACATGGGAA661942.0797260796469046No Hit
GCTTCCCATGTACTCTGCGTTGATA541411.7010370981986747No Hit
GTACTCTGCGTTGATACCACTGCTT353461.1105235823669741No Hit
GTGGTATCAACGCAGAGTACATGGG328051.0306887941930796No Hit
ATACCACTGCTTCCCATGTACTCTG324951.0209490128731633No Hit
CAGTGGTATCAACGCAGAGTACATG308900.9705220805555319No Hit
GGGAAGCAGTGGTATCAACGCAGAG298040.9364014272864059No Hit
ACATGGGAAGCAGTGGTATCAACGC295590.92870385817873No Hit
GTTGATACCACTGCTTCCCATGTAC283560.8909072229275709No Hit
GCAGTGGTATCAACGCAGAGTACAT280600.8816073026995217No Hit
TACCACTGCTTCCCATGTACTCTGC278040.873564128448236No Hit
CATGGGAAGCAGTGGTATCAACGCA274250.8616564603184029No Hit
CTGCTTCCCATGTACTCTGCGTTGA273510.8593314802613905No Hit
GATACCACTGCTTCCCATGTACTCT268260.8428366893163711No Hit
GCAGAGTACATGGGAAGCAGTGGTA253210.7955516219406483No Hit
CCATGTACTCTGCGTTGATACCACT236720.7437422690485773No Hit
CTTCCCATGTACTCTGCGTTGATAC236490.7430196401119383No Hit
CCACTGCTTCCCATGTACTCTGCGT236450.742893965514262No Hit
AAGCAGTGGTATCAACGCAGAGTAC223170.7011699990857173No Hit
GTACATGGTAAGCAGTGGTATCAAC218960.6879427476802826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA199850.6279017086404114No Hit
TTCCCATGTACTCTGCGTTGATACC192660.6053116997080893No Hit
ATGGGAAGCAGTGGTATCAACGCAG192490.6047775826679649No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA174720.5489466426502511No Hit
ACCATGTACTCTGCGTTGATACCAC172820.542977099260625No Hit
TGATACCACTGCTTCCCATGTACTC168240.5285873578266841No Hit
ATCAACGCAGAGTACATGGGAAGCA131710.41381503149876697No Hit
CCCCATGTACTCTGCGTTGATACCA105960.3329120092446234No Hit
TCCATGTACTCTGCGTTGATACCAC96650.30366124663545535No Hit
GGTAAGCAGTGGTATCAACGCAGAG94730.29762886594699106No Hit
GGAAGCAGTGGTATCAACGCAGAGT89580.28144826149616237No Hit
GTACATGGGTAAGCAGTGGTATCAA88200.27711248787632864No Hit
GTACTTTTTTTTTTTTTTTTTTTTT80230.252071824289318No Hit
AAAAAGTACTCTGCGTTGATACCAC79830.2508150783125546No Hit
GAGTACATGGTAAGCAGTGGTATCA77530.2435887889461651No Hit
GAACAAAAAAAAAAAAAAAAAAAAA75870.238373293142597No Hit
GCTTACCATGTACTCTGCGTTGATA72010.22624569446683024No Hit
CTGCGTTGATACCACTGCTTCCCAT70370.22109303596210028No Hit
CTCTGCGTTGATACCACTGCTTCCC66960.2103792765101924No Hit
GTACATGGAAAGCAGTGGTATCAAC66890.21015934596425875No Hit
TCCCATGTACTCTGCGTTGATACCA64550.20280738200019294No Hit
TGGGAAGCAGTGGTATCAACGCAGA63500.199508423811189No Hit
TCAACGCAGAGTACATGGGAAGCAG60250.18929736274998643No Hit
AAAGTACTCTGCGTTGATACCACTG58920.18511868237724813No Hit
ACTCTGCGTTGATACCACTGCTTAC55080.17305392100031952No Hit
GCGTTGATACCACTGCTTACCATGT54820.17223703611542332No Hit
AGTGGTATCAACGCAGAGTACATGG53060.16670735381766438No Hit
GAGTACATGGAAAGCAGTGGTATCA48480.1523176123837235No Hit
GTACATGGAAGCAGTGGTATCAACG46550.14625381304584012No Hit
TATCAACGCAGAGTACATGGTAAGC46110.14487139247140038No Hit
ACGCAGAGTACATGGTAAGCAGTGG45830.14399167028766602No Hit
GTATCAACGCAGAGTACTTTTTTTT45590.14323762270160798No Hit
CTGCTTACCATGTACTCTGCGTTGA44900.1410697358916911No Hit
ACTGCTTCCCATGTACTCTGCGTTG44800.14075554939750026No Hit
AACGCAGAGTACATGGGAAGCAGTG43590.136953892817791No Hit
TTGATACCACTGCTTCCCATGTACT43560.13685963686953373No Hit
GTACAAAAAAAAAAAAAAAAAAAAA43380.13629410117999022No Hit
ATGGTAAGCAGTGGTATCAACGCAG41330.1298532780490778No Hit
GTATCAACGCAGAGTACATGGTAAG40360.12680566905542656No Hit
TATCAACGCAGAGTACTTTTTTTTT39630.1245121076478334No Hit
GCTTTCCATGTACTCTGCGTTGATA39560.1242921771018998No Hit
GGTATCAACGCAGAGTACATGGTAA38920.12228138353907836No Hit
GGTATCAACGCAGAGTACTTTTTTT38590.12124456810824855No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36650.11514935012094608No Hit
ATGTACTCTGCGTTGATACCACTGC36650.11514935012094608No Hit
CGTTGATACCACTGCTTCCCATGTA35790.11244734627090479No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA35380.11115918164472231No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN33980.10676057072605041No Hit
CAACGCAGAGTACATGGGAAGCAGT32620.10248763440505487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA19200.016.9880661
TACATAA8250.016.3596232
GTACATA12350.016.169751
TACAAAA19650.015.7686272
TACAAGA4050.015.7238412
GTAAAAA9050.015.5512091
AGAACAA17950.014.4556312
GTAGATG1400.014.2640311
GTACAAG6050.014.1461461
TACATAG4900.013.7721312
GTAAATA1800.013.2074371
CATGAAG1102.7266651E-912.9623984
GGGGAGC3450.012.9409047
TACATGA7200.012.8049562
TCGAAAC604.1041998E-412.66297316
GTGTCGA604.10529E-412.66257413
AGTACAA3400.012.5797632
GGAGCAG3550.012.5761739
TAGATGG1457.2759576E-1212.4544482
TACAAAG2550.012.3002132
Produced by FastQC (version 0.11.5)