FastQCFastQC Report
Wed 25 May 2016
SRR1295026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182823
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC32747310.288759381215984No Hit
GTACATGGGAAGCAGTGGTATCAAC2955779.286630139344853No Hit
GAGTACATGGGAAGCAGTGGTATCA1502584.720903424412856No Hit
CATGTACTCTGCGTTGATACCACTG1231893.870432003287647No Hit
GCGTTGATACCACTGCTTCCCATGT992183.117295558062764No Hit
ACGCAGAGTACATGGGAAGCAGTGG945432.970413372028542No Hit
TATCAACGCAGAGTACATGGGAAGC834182.6208808972412228No Hit
ACTCTGCGTTGATACCACTGCTTCC774502.433374397508124No Hit
GTATCAACGCAGAGTACATGGGAAG734922.309019383107386No Hit
GGTATCAACGCAGAGTACATGGGAA718422.2571786115658963No Hit
GCTTCCCATGTACTCTGCGTTGATA599781.8844277548578732No Hit
CAGTGGTATCAACGCAGAGTACATG379581.1925890946496238No Hit
ACATGGGAAGCAGTGGTATCAACGC371001.1656318934480492No Hit
GTGGTATCAACGCAGAGTACATGGG353661.111151955355356No Hit
CATGGGAAGCAGTGGTATCAACGCA348731.095662561191747No Hit
GGGAAGCAGTGGTATCAACGCAGAG344101.0811157265107108No Hit
GTACTCTGCGTTGATACCACTGCTT341431.0727269471158152No Hit
ATACCACTGCTTCCCATGTACTCTG340151.0687053599901721No Hit
GCAGTGGTATCAACGCAGAGTACAT325101.0214202926144496No Hit
CTGCTTCCCATGTACTCTGCGTTGA302130.9492516548988115No Hit
GATACCACTGCTTCCCATGTACTCT295440.9282325784374438No Hit
TACCACTGCTTCCCATGTACTCTGC292000.9174245630372786No Hit
GCAGAGTACATGGGAAGCAGTGGTA287960.9047314286719682No Hit
CCATGTACTCTGCGTTGATACCACT279990.8796907650849575No Hit
GTTGATACCACTGCTTCCCATGTAC275700.8662121644841703No Hit
CCACTGCTTCCCATGTACTCTGCGT272490.8561267780206439No Hit
CTTCCCATGTACTCTGCGTTGATAC270430.8496545362403124No Hit
AAGCAGTGGTATCAACGCAGAGTAC258280.8114808771961243No Hit
GTACTTTTTTTTTTTTTTTTTTTTT243600.7653582998489077No Hit
ATGGGAAGCAGTGGTATCAACGCAG227270.7140516453475421No Hit
GTACATGGTAAGCAGTGGTATCAAC224920.7066682627340571No Hit
ACCATGTACTCTGCGTTGATACCAC189410.5951006386468868No Hit
TTCCCATGTACTCTGCGTTGATACC186980.5874659068380491No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA176950.555953001470707No Hit
TGATACCACTGCTTCCCATGTACTC165260.5192246002997968No Hit
GTATCAACGCAGAGTACTTTTTTTT160700.504897696164694No Hit
TATCAACGCAGAGTACTTTTTTTTT158960.4994308511657733No Hit
ATCAACGCAGAGTACATGGGAAGCA154310.4848211791858988No Hit
GGTATCAACGCAGAGTACTTTTTTT140230.44058372080382735No Hit
CCCCATGTACTCTGCGTTGATACCA121080.38041700716627974No Hit
GAGTACTTTTTTTTTTTTTTTTTTT115990.3644249146119655No Hit
ACGCAGAGTACTTTTTTTTTTTTTT115500.3628854007904304No Hit
GGTAAGCAGTGGTATCAACGCAGAG110220.3462963538971536No Hit
GGAAGCAGTGGTATCAACGCAGAGT106540.3347342909109303No Hit
TCCATGTACTCTGCGTTGATACCAC100690.3163543810007657No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA99710.31327535335769535No Hit
GTACATGGGTAAGCAGTGGTATCAA97020.3048237366639615No Hit
GAGTACATGGTAAGCAGTGGTATCA86130.2706088274465781No Hit
AAAAAGTACTCTGCGTTGATACCAC84530.2655818435395245No Hit
CTGCGTTGATACCACTGCTTCCCAT79200.24883570339915229No Hit
GCTTACCATGTACTCTGCGTTGATA78310.2460394436008537No Hit
CTCTGCGTTGATACCACTGCTTCCC74730.23479156710882132No Hit
TGGGAAGCAGTGGTATCAACGCAGA71250.22385787711097976No Hit
TCAACGCAGAGTACATGGGAAGCAG70510.2215328970539675No Hit
GTACATGGAAAGCAGTGGTATCAAC66620.2093110424299435No Hit
TCCCATGTACTCTGCGTTGATACCA66590.20921678648168623No Hit
AAAGTACTCTGCGTTGATACCACTG62810.19734053700127216No Hit
ACTCTGCGTTGATACCACTGCTTAC62280.19567534858206062No Hit
AGTGGTATCAACGCAGAGTACATGG61950.19463853315123084No Hit
GCGTTGATACCACTGCTTACCATGT59440.18675245214704053No Hit
ACGCAGAGTACATGGTAAGCAGTGG56840.17858360329807846No Hit
GAGTACATGGAAAGCAGTGGTATCA53700.1687181473804858No Hit
AACGCAGAGTACATGGGAAGCAGTG53610.16843537953571405No Hit
ACTGCTTCCCATGTACTCTGCGTTG49240.15470542973957396No Hit
CTGCTTACCATGTACTCTGCGTTGA48480.1523176123837235No Hit
ATGGTAAGCAGTGGTATCAACGCAG48430.15216051913662806No Hit
TATCAACGCAGAGTACATGGTAAGC47660.14974128313135854No Hit
GTACATGGAAGCAGTGGTATCAACG45440.1427663429603217No Hit
GCTTTCCATGTACTCTGCGTTGATA44180.138807593133517No Hit
CAACGCAGAGTACATGGGAAGCAGT42780.13440898221484512No Hit
GGTATCAACGCAGAGTACATGGTAA42280.13283804974389088No Hit
CGTTGATACCACTGCTTCCCATGTA41750.1311728613246794No Hit
GTATCAACGCAGAGTACATGGTAAG41650.13085867483048852No Hit
TTGATACCACTGCTTCCCATGTACT41450.13023030184210685No Hit
CGCAGAGTACATGGGAAGCAGTGGT40390.12689992500368383No Hit
GCAGAGTACTTTTTTTTTTTTTTTT37110.11659460799422398No Hit
ATGTACTCTGCGTTGATACCACTGC37060.11643751474712857No Hit
GAACAAAAAAAAAAAAAAAAAAAAA35600.11185039193194218No Hit
ACGCAGAGTACATGGAAAGCAGTGG34970.10987101701853982No Hit
GCGTTGATACCACTGCTTTCCATGT33040.10380721768065646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGT307.7285565E-418.9987583
TAGGCTA250.006035714418.9987585
GTACAAA7250.017.972291
TCTGTCG350.002171818916.284658
GATACGA1001.8189894E-1216.14894518
TACAAAA7600.015.6244712
TACAAGA1400.015.6066142
GGGACGC2700.015.4804717
TGGGACG2700.015.4804716
GGACGCA2650.015.4140878
ATACGAC1055.456968E-1215.37994819
TGATACG1004.0017767E-1115.19900617
CTGTCGC456.7684357E-414.7768139
GTACAAG2450.014.75157451
CGCAAAA4150.014.4211972
AGAGGCG400.005283000414.24906919
GGTATCG759.689011E-713.93242317
GTAAAAA2050.013.9183661
TACAAAG1450.013.7581542
TAACAAA707.182809E-613.5869771
Produced by FastQC (version 0.11.5)