FastQCFastQC Report
Wed 25 May 2016
SRR1295025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432619
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2301719.461859830906524No Hit
CCCATGTACTCTGCGTTGATACCAC2217879.117210709938547No Hit
GAGTACATGGGAAGCAGTGGTATCA1078644.43406879581225No Hit
CATGTACTCTGCGTTGATACCACTG854823.5139904769304198No Hit
GCGTTGATACCACTGCTTCCCATGT692342.8460683732224408No Hit
TATCAACGCAGAGTACATGGGAAGC592342.4349887919152158No Hit
ACGCAGAGTACATGGGAAGCAGTGG585512.406912056511932No Hit
ACTCTGCGTTGATACCACTGCTTCC532802.190232009204894No Hit
GGTATCAACGCAGAGTACATGGGAA527632.1689791948513104No Hit
GTATCAACGCAGAGTACATGGGAAG521102.1421356981919484No Hit
GCTTCCCATGTACTCTGCGTTGATA398361.6375766200954611No Hit
GTACTCTGCGTTGATACCACTGCTT272261.1192052680670503No Hit
GTGGTATCAACGCAGAGTACATGGG257831.0598864844844178No Hit
ATACCACTGCTTCCCATGTACTCTG248211.0203406287626626No Hit
CAGTGGTATCAACGCAGAGTACATG232310.954978975334814No Hit
ACATGGGAAGCAGTGGTATCAACGC232250.9547323275860297No Hit
GGGAAGCAGTGGTATCAACGCAGAG220110.9048272664153325No Hit
GTTGATACCACTGCTTCCCATGTAC219540.9024841128018815No Hit
GCAGTGGTATCAACGCAGAGTACAT216470.8898639696557495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA213700.8784770652535394No Hit
CTGCTTCCCATGTACTCTGCGTTGA211770.87054322933431No Hit
TACCACTGCTTCCCATGTACTCTGC211160.8680356438883361No Hit
GATACCACTGCTTCCCATGTACTCT209710.8620749899593814No Hit
CATGGGAAGCAGTGGTATCAACGCA204940.8424664939310267No Hit
GCAGAGTACATGGGAAGCAGTGGTA186480.766581203221713No Hit
CCACTGCTTCCCATGTACTCTGCGT184120.7568797251028624No Hit
CTTCCCATGTACTCTGCGTTGATAC183560.754577679447542No Hit
CCATGTACTCTGCGTTGATACCACT179990.7399021383948741No Hit
AAGCAGTGGTATCAACGCAGAGTAC166190.6831731561744769No Hit
GTACATGGTAAGCAGTGGTATCAAC164930.677993553450006No Hit
TTCCCATGTACTCTGCGTTGATACC152540.6270607933260407No Hit
ATGGGAAGCAGTGGTATCAACGCAG148540.6106176100737518No Hit
ACCATGTACTCTGCGTTGATACCAC135320.5562728894249367No Hit
TGATACCACTGCTTCCCATGTACTC130510.5364999615640591No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA127440.5238798184179274No Hit
ATCAACGCAGAGTACATGGGAAGCA92610.3807008002486209No Hit
CCCCATGTACTCTGCGTTGATACCA77240.3175178686017005No Hit
GGTAAGCAGTGGTATCAACGCAGAG70750.2908388037748616No Hit
TCCATGTACTCTGCGTTGATACCAC70520.28989332073785495No Hit
GAACAAAAAAAAAAAAAAAAAAAAA69970.28763238304066524No Hit
GGAAGCAGTGGTATCAACGCAGAGT66880.274930023978272No Hit
GTACATGGGTAAGCAGTGGTATCAA66850.2748067001038798No Hit
GAGTACATGGTAAGCAGTGGTATCA58080.23875502082323619No Hit
AAAAAGTACTCTGCGTTGATACCAC57410.23600078762847776No Hit
GCTTACCATGTACTCTGCGTTGATA54600.22444945139374478No Hit
CTGCGTTGATACCACTGCTTCCCAT50900.20923950688537746No Hit
CTCTGCGTTGATACCACTGCTTCCC50350.20697856918818772No Hit
GTACATGGAAAGCAGTGGTATCAAC50110.20599197819305037No Hit
TCCCATGTACTCTGCGTTGATACCA46820.19246745996804268No Hit
TCAACGCAGAGTACATGGGAAGCAG46290.1902887381871144No Hit
TGGGAAGCAGTGGTATCAACGCAGA44590.18330038530489154No Hit
AAAGTACTCTGCGTTGATACCACTG43660.17947734519873437No Hit
ACTCTGCGTTGATACCACTGCTTAC42230.17359890718604104No Hit
GCGTTGATACCACTGCTTACCATGT41900.17224234456772722No Hit
AGTGGTATCAACGCAGAGTACATGG41820.17191348090268144No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40890.16809044079652424No Hit
GAGTACATGGAAAGCAGTGGTATCA38020.15629245681300688No Hit
ACGCAGAGTACATGGTAAGCAGTGG37440.153908195241425No Hit
CTGCTTACCATGTACTCTGCGTTGA34890.14342566591809075No Hit
TATCAACGCAGAGTACATGGTAAGC34510.1418635635091233No Hit
TTGATACCACTGCTTCCCATGTACT34000.13976705764445646No Hit
AACGCAGAGTACATGGGAAGCAGTG33810.13898600643997272No Hit
ACTGCTTCCCATGTACTCTGCGTTG33650.13832827910988116No Hit
GTATCAACGCAGAGTACTTTTTTTT32000.13154546601831196No Hit
GGTATCAACGCAGAGTACATGGTAA31790.13068219889756677No Hit
ATGGTAAGCAGTGGTATCAACGCAG31140.12801018161906982No Hit
GTACATGGAAGCAGTGGTATCAACG30730.1263247553357102No Hit
GCTTTCCATGTACTCTGCGTTGATA30700.12620143146131801No Hit
ATGTACTCTGCGTTGATACCACTGC30360.12480376088487347No Hit
GTATCAACGCAGAGTACATGGTAAG29320.12052853323927831No Hit
TATCAACGCAGAGTACTTTTTTTTT29270.12032299344862471No Hit
GGTATCAACGCAGAGTACTTTTTTT28270.11621219763555246No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA26820.1102515437065977No Hit
CGTTGATACCACTGCTTCCCATGTA26470.10881276517202242No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN25910.10651071951670195No Hit
AAACAAAAAAAAAAAAAAAAAAAAA25790.10601742401913329No Hit
CAACGCAGAGTACATGGGAAGCAGT24670.10141333270849237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCC250.006017671419.0083893
GTACAAA9200.017.0564631
TATTCCA402.753067E-416.6319985
TACAAAA11750.014.5596182
TACATAA5900.014.4979232
AGAACAA14850.014.4642962
GTACATA6750.014.3710761
CGATGTA400.005264792614.2562912
GTAAAAA4300.013.9336521
ATGTACG1450.013.7655474
TGTACGC1450.013.7644125
GTACGCT1450.013.7564736
TACATGA7600.013.756072
CGGTGGT1550.013.48037613
CGCAAAA6750.013.3762732
ACATGAA9150.013.29548553
GTATCAT1153.8016879E-1013.21369618
TACGCTG1403.6379788E-1212.8919067
TATCTAC751.4833402E-512.66338519
GGTGGTA1700.012.29093214
Produced by FastQC (version 0.11.5)