##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295025_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2432619
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.330872199880048	33.0	33.0	33.0	22.0	33.0
2	30.190725715782044	33.0	33.0	33.0	22.0	33.0
3	30.510938211039214	33.0	33.0	33.0	27.0	33.0
4	33.43179634788678	37.0	33.0	37.0	22.0	37.0
5	34.532832309539636	37.0	37.0	37.0	27.0	37.0
6	33.93491089233456	37.0	37.0	37.0	27.0	37.0
7	34.225431109433906	37.0	37.0	37.0	27.0	37.0
8	34.007746383630156	37.0	37.0	37.0	27.0	37.0
9	33.83269677660168	37.0	37.0	37.0	22.0	37.0
10	34.06293176202274	37.0	37.0	37.0	27.0	37.0
11	34.229297723975684	37.0	37.0	37.0	27.0	37.0
12	34.274766414304914	37.0	37.0	37.0	27.0	37.0
13	33.843490904247645	37.0	37.0	37.0	27.0	37.0
14	35.441494948448565	40.0	37.0	40.0	27.0	40.0
15	35.30876392891776	37.0	37.0	40.0	27.0	40.0
16	35.29482298707689	37.0	33.0	40.0	27.0	40.0
17	35.4200217954394	37.0	37.0	40.0	27.0	40.0
18	35.22972442458108	37.0	33.0	40.0	27.0	40.0
19	35.05106142803292	37.0	33.0	40.0	27.0	40.0
20	35.220763300788164	37.0	33.0	40.0	27.0	40.0
21	35.13592223032049	37.0	33.0	40.0	27.0	40.0
22	35.22455550992572	37.0	33.0	40.0	27.0	40.0
23	35.25112687190226	37.0	33.0	40.0	27.0	40.0
24	35.409120787102296	37.0	33.0	40.0	27.0	40.0
25	34.26433198129259	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6518121760927968
1101	2	0.5298320405946058
1101	3	0.5547118210678512
1101	4	0.689637871151703
1101	5	0.5242785372359933
1101	6	0.4155128495660563
1101	7	0.35916647281426606
1101	8	0.42514038697149203
1101	9	0.41656162221406845
1101	10	0.4813263322511787
1101	11	0.370030894182392
1101	12	0.4330412691867025
1101	13	0.5262169143257438
1101	14	0.9426339360524736
1101	15	0.8248292824583245
1101	16	0.859520301133962
1101	17	0.8097123090436043
1101	18	0.906304773590243
1101	19	0.6766010286904134
1101	20	0.608155441588039
1101	21	0.7217935861651412
1101	22	0.7769809487686388
1101	23	0.785731159027236
1101	24	0.8716964481823979
1101	25	0.9841335425281059
1102	1	0.4646502791075626
1102	2	0.3718419436675511
1102	3	0.40769556145457386
1102	4	0.3705565577510228
1102	5	0.2259665544985623
1102	6	0.35244101850387466
1102	7	0.19305830955971715
1102	8	0.3743012421837335
1102	9	0.2679175324069121
1102	10	0.4005105770400377
1102	11	0.36898241244432484
1102	12	0.31344894073781404
1102	13	0.34677391306114913
1102	14	0.7492789047058466
1102	15	0.7568684500558263
1102	16	0.7178535506079271
1102	17	0.7104700144020271
1102	18	0.7734322355216818
1102	19	0.7318859181421971
1102	20	0.7275697485717956
1102	21	0.6599314864319155
1102	22	0.6532339874728521
1102	23	0.8594641517477655
1102	24	0.7878706207419839
1102	25	0.8955914640520248
1103	1	0.37222636188525016
1103	2	0.3576717449863267
1103	3	0.30416393999847813
1103	4	0.29204315653696966
1103	5	0.14385501910019372
1103	6	0.2242731139614378
1103	7	0.19639369488147196
1103	8	0.33916412454347267
1103	9	0.33481675776162945
1103	10	0.48545841643774423
1103	11	0.32252154213392714
1103	12	0.3963320552316958
1103	13	0.2269310277610117
1103	14	0.6871345532167368
1103	15	0.5298131006163516
1103	16	0.6107794581017743
1103	17	0.5731457601347145
1103	18	0.5734364810149728
1103	19	0.6695432863923543
1103	20	0.6376123843312698
1103	21	0.7229407392486777
1103	22	0.6411286709359487
1103	23	0.7270253595748812
1103	24	0.7748621934377979
1103	25	0.8290281043887759
1104	1	0.41253154391099045
1104	2	0.25965935954464214
1104	3	0.41984426174850853
1104	4	0.4277539153656349
1104	5	0.2868717688538922
1104	6	0.2757092027266239
1104	7	0.25143800493405877
1104	8	0.44167623658187694
1104	9	0.3986736239281896
1104	10	0.46958924751351816
1104	11	0.39649573380015823
1104	12	0.3917058844923176
1104	13	0.5101192610453822
1104	14	0.8018671289252666
1104	15	0.6779966653739251
1104	16	0.5498269070268407
1104	17	0.6423215486945253
1104	18	0.7242084455170072
1104	19	0.7129229877782493
1104	20	0.6662512416069575
1104	21	0.7342219592903518
1104	22	0.7703188589952887
1104	23	0.7809311263170287
1104	24	0.7223510564467688
1104	25	0.8984099789602382
1105	1	0.40433694553571087
1105	2	0.21463287931647557
1105	3	0.2107479412477673
1105	4	0.35145802138831783
1105	5	0.2876347656883027
1105	6	0.24498523968425445
1105	7	0.19557304098925243
1105	8	0.32106512143640487
1105	9	0.26699572923644155
1105	10	0.4403085286353132
1105	11	0.3321289929450799
1105	12	0.358222805489973
1105	13	0.4092851228377796
1105	14	0.7917870792198798
1105	15	0.46270356701516135
1105	16	0.5442451822626779
1105	17	0.36472951906494444
1105	18	0.4216085875522779
1105	19	0.5429691163736052
1105	20	0.4975471197303918
1105	21	0.3508882044758934
1105	22	0.39139418339460974
1105	23	0.5555777762652028
1105	24	0.5723452291802502
1105	25	0.6322571789838136
1106	1	0.2567257883967997
1106	2	-0.03886667191246573
1106	3	0.06217451539855645
1106	4	0.22399841078351557
1106	5	-0.3643303337317505
1106	6	-0.21288637727238324
1106	7	-0.6020355270858673
1106	8	-0.12305425166495354
1106	9	0.15718058839136972
1106	10	-0.3688860999203314
1106	11	-0.1865122663755514
1106	12	-0.7196950694064626
1106	13	-0.05803657696890241
1106	14	-0.2544536001504625
1106	15	0.04926459987900955
1106	16	0.0533802623510482
1106	17	-0.06392019786408554
1106	18	-0.2389090526023736
1106	19	-0.39270990875757406
1106	20	0.20755902942384807
1106	21	0.27550761209809593
1106	22	0.3640105186611109
1106	23	-0.4100653471813587
1106	24	0.34101237913882443
1106	25	0.4994943027065375
1107	1	-1.1581295927864872
1107	2	-1.187974245874365
1107	3	-0.9577124277247542
1107	4	-1.2113318873878995
1107	5	-1.2044192745622269
1107	6	-1.0886249150498841
1107	7	-1.2543026839486089
1107	8	-1.1938972352897963
1107	9	-1.2182348744094966
1107	10	-1.0621977378491394
1107	11	-0.9786649978921744
1107	12	-1.2191171008606005
1107	13	-1.05952632054872
1107	14	-1.3393125832485708
1107	15	-1.2639693386792956
1107	16	-1.214290986751699
1107	17	-1.2441273692894086
1107	18	-1.3411662364213868
1107	19	-1.3403141512710093
1107	20	-1.3969267585046978
1107	21	-1.478617887522347
1107	22	-1.3704316944896178
1107	23	-1.1695825033986011
1107	24	-1.0618083784768402
1107	25	-1.1465048445820827
1108	1	0.03882231931686064
1108	2	-0.130132697553492
1108	3	0.014011808007907689
1108	4	-0.17528703416369495
1108	5	-0.33556430809072424
1108	6	-0.13624327772487277
1108	7	-0.1555847352306614
1108	8	0.050096200823738
1108	9	-0.2941061763145072
1108	10	-0.42297169569710036
1108	11	-0.06853338251431751
1108	12	-0.06801049172923967
1108	13	0.0010443053840347716
1108	14	-0.3340275066361329
1108	15	0.020060225821694644
1108	16	-0.8231195491120005
1108	17	-0.9216624678489964
1108	18	-0.710073268288653
1108	19	-0.10231586652529501
1108	20	-0.9559166493091809
1108	21	-0.13270600026992696
1108	22	-0.19012409672350117
1108	23	-0.05915848443626004
1108	24	-0.21363505675861205
1108	25	-0.9812475902044682
1109	1	0.22055646236679394
1109	2	0.1943274913803812
1109	3	0.19570209140871597
1109	4	0.3546421156338724
1109	5	0.13341342470280182
1109	6	0.22324052794314042
1109	7	0.1659908383185993
1109	8	0.3455617878682702
1109	9	0.326507858773212
1109	10	0.21802695477523315
1109	11	0.20823449872725064
1109	12	0.30224591852717
1109	13	0.22233647812358015
1109	14	0.47466345152851375
1109	15	0.37647048223195156
1109	16	0.3897785615492708
1109	17	0.43079704632103955
1109	18	0.4067890798894851
1109	19	0.35374013887281563
1109	20	0.31751452477988806
1109	21	0.3731610712550406
1109	22	0.37358373053695715
1109	23	0.4295864548349897
1109	24	0.47364564499577
1109	25	0.5641910062867552
1110	1	0.02886300926580887
1110	2	-0.04783822947920768
1110	3	0.15111303061371828
1110	4	-1.040203206511059
1110	5	-0.3071058213753517
1110	6	-0.10519919906611364
1110	7	0.14862317769284772
1110	8	0.20728706847332035
1110	9	0.151323198013948
1110	10	-0.041989097008084286
1110	11	0.0511981909787238
1110	12	0.07267831281277637
1110	13	0.1875522812498147
1110	14	0.2952421087802719
1110	15	0.15362148644717877
1110	16	0.08125423893151407
1110	17	0.22081764811735383
1110	18	0.3136956382325522
1110	19	0.32151127748533526
1110	20	0.2479151994767932
1110	21	0.32001018097836464
1110	22	-0.1791671199867082
1110	23	0.1987339033643849
1110	24	0.33957372718411705
1110	25	0.5378270313472129
1111	1	-0.14123189166921435
1111	2	-0.27727056302803277
1111	3	-0.11956457552839694
1111	4	-0.10384723148141006
1111	5	-0.20387400440642978
1111	6	-0.12012180400820682
1111	7	-0.10600609076223577
1111	8	-0.0229606821730286
1111	9	-0.10063590243341736
1111	10	-0.05255368017888884
1111	11	-0.21384143451575
1111	12	-0.12401044034442066
1111	13	-0.25977399788917666
1111	14	-0.36860101262246303
1111	15	-0.32393031937125727
1111	16	-0.31983246211749616
1111	17	-0.25644433928417953
1111	18	-0.35094501177562876
1111	19	-0.4320201468808733
1111	20	-0.2377341304252596
1111	21	-0.40825145014403574
1111	22	-0.2949532421939267
1111	23	-0.07821660066872482
1111	24	-0.07830542313238453
1111	25	-0.057608260262533406
1112	1	-0.2248801644810463
1112	2	-0.624632769702206
1112	3	-0.24905269294257337
1112	4	-0.2940860403935801
1112	5	-0.13696179731547176
1112	6	-0.17215846847980032
1112	7	-0.2315476068749618
1112	8	-0.19569175724217303
1112	9	-0.29045639244696275
1112	10	0.01386549288708494
1112	11	-0.2607841087068081
1112	12	-0.23851183118580366
1112	13	-0.36060319481985914
1112	14	-0.42885431835308196
1112	15	-0.3160301294636696
1112	16	-0.4608366383191793
1112	17	-0.5173502960472263
1112	18	-0.44397588634590335
1112	19	-0.3076981606034508
1112	20	-1.0689523535120031
1112	21	-1.3495195993614786
1112	22	-1.7663902439194246
1112	23	-0.727100740369707
1112	24	-0.4003907486647833
1112	25	-1.4499701187973173
1113	1	-0.13301873817637144
1113	2	-0.13491849146972257
1113	3	-0.21228537307188233
1113	4	-0.5382731784541903
1113	5	-0.22096803479879412
1113	6	-0.18623372206314315
1113	7	-0.15205710344216072
1113	8	0.0033307923191969735
1113	9	-0.18011509493212685
1113	10	-0.06959217666540951
1113	11	-0.23460441293317302
1113	12	-0.1408978941818546
1113	13	-0.18517005996076108
1113	14	-0.632592564766405
1113	15	-0.6936441857088056
1113	16	-0.4275024126252944
1113	17	-0.5382272074572185
1113	18	-0.8851521024979192
1113	19	-1.1939705886147962
1113	20	-1.273690180126188
1113	21	-1.0300957120074656
1113	22	-0.45965268407817916
1113	23	-1.9544379875418514
1113	24	-1.0485052590201178
1113	25	-0.6200069467899141
1114	1	-0.6688129076469878
1114	2	-0.6390617543624089
1114	3	-0.6614036128492096
1114	4	-0.8891377182263938
1114	5	-0.9159167589378754
1114	6	-0.8267995883165185
1114	7	-0.7980011527354165
1114	8	-0.4979983900977061
1114	9	-1.6552958111790588
1114	10	-0.6727578694762073
1114	11	-0.7862628379822851
1114	12	-0.6092517710623895
1114	13	-0.7721476851931115
1114	14	-1.2148438516574203
1114	15	-1.635720330488894
1114	16	-1.7084652046343294
1114	17	-1.9687613171739997
1114	18	-2.1974937629882945
1114	19	-2.0390468622668863
1114	20	-1.2557535615135862
1114	21	-1.0171972569519951
1114	22	-0.7807112127876508
1114	23	-0.6372032429757226
1114	24	-1.184510294594979
1114	25	-0.7872827446848518
1115	1	-0.7395574173032209
1115	2	-0.5718974897474709
1115	3	-0.6175416933310025
1115	4	-0.740827916743207
1115	5	-0.4612768644899745
1115	6	-0.4686048932195348
1115	7	-0.5983656310158452
1115	8	-0.44094448000235076
1115	9	-0.7796808067389236
1115	10	-0.37868388527195407
1115	11	-0.4792974777419303
1115	12	-0.733262683505032
1115	13	-0.722761130953586
1115	14	-0.6936331704048868
1115	15	-1.7390516923031
1115	16	-1.1162041755013448
1115	17	-0.8905260458542088
1115	18	-2.490120955460803
1115	19	-1.5803247436758028
1115	20	-1.1034424187233611
1115	21	-1.0156116163515065
1115	22	-0.7990647078529562
1115	23	-0.38809506049729947
1115	24	-0.4815764382193066
1115	25	-0.8423653041548036
1116	1	-1.681462475838785
1116	2	-0.9717118441141892
1116	3	-1.8469703095055756
1116	4	-1.2121580721048417
1116	5	-1.2214144358227657
1116	6	-0.8295908035078199
1116	7	-1.6491366322880907
1116	8	-3.238884781293777
1116	9	-1.9022857581103274
1116	10	-1.0759880643223312
1116	11	-0.7936029255174333
1116	12	-1.0294978604490765
1116	13	-2.061292717986195
1116	14	-1.9179701691396787
1116	15	-1.8426562090349634
1116	16	-1.318485241230455
1116	17	-2.0361207893552944
1116	18	-2.7505898734547003
1116	19	-1.7200564285463003
1116	20	-1.316073712418273
1116	21	-1.053967235959206
1116	22	-0.8254036879809519
1116	23	-0.747374966601086
1116	24	-0.9358557020419909
1116	25	-0.9050596821715402
1201	1	0.5051731501741301
1201	2	0.4827043223650733
1201	3	0.5183190601263199
1201	4	0.6202593507818293
1201	5	0.5560335790007258
1201	6	0.2784623456931996
1201	7	0.23153422592263695
1201	8	0.1590078328255018
1201	9	0.20868352325630468
1201	10	0.48249875659737995
1201	11	0.3367730992728539
1201	12	0.41275543762038325
1201	13	0.4908297131093562
1201	14	0.730210415670463
1201	15	0.7964275637770157
1201	16	0.7242822336545913
1201	17	0.7760452464681578
1201	18	0.764003984930234
1201	19	0.6279251371062671
1201	20	0.6525900085537018
1201	21	0.7674117503628466
1201	22	0.8347454972018866
1201	23	0.9491963081245416
1201	24	0.7692580872933306
1201	25	0.9334096123926443
1202	1	0.3989641163927544
1202	2	0.4238273347733035
1202	3	0.3906130863856916
1202	4	0.4708293422965184
1202	5	0.3292913233838206
1202	6	-0.03464971369577796
1202	7	0.1259265424124223
1202	8	0.20369549551185884
1202	9	0.23172954479341712
1202	10	0.31343488721378066
1202	11	0.29007068772299505
1202	12	0.24531402520559453
1202	13	0.3340720035693252
1202	14	0.7256808175134566
1202	15	0.5485335194812322
1202	16	0.6189508351360402
1202	17	0.4615136397110646
1202	18	0.7036230067829834
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2211	1	0.10316141691177094
2211	2	0.07444889656298415
2211	3	0.13228252953444297
2211	4	0.14230191435548534
2211	5	0.009466500893573482
2211	6	0.19631287413360354
2211	7	0.226575869022966
2211	8	0.31146372219125595
2211	9	0.16080338440900732
2211	10	0.3255136948813089
2211	11	0.15236902257413476
2211	12	0.17135742717356806
2211	13	0.11675489969928066
2211	14	-0.04508097513146225
2211	15	0.046625520991639746
2211	16	-0.031145786440561096
2211	17	0.08189746059464653
2211	18	-0.018497040898473927
2211	19	-0.0011828909737161553
2211	20	0.09407341230878785
2211	21	0.14058161650606138
2211	22	0.05727602253708142
2211	23	0.24347717447846406
2211	24	0.2306603119553614
2211	25	0.07587226668282909
2212	1	0.05617274666728633
2212	2	0.06919575177495574
2212	3	0.023389097385198454
2212	4	0.08870563982898005
2212	5	0.1034466809202641
2212	6	0.13641850493691265
2212	7	0.18403675439083145
2212	8	0.21219632292385882
2212	9	0.022682590474254027
2212	10	0.2683225655971597
2212	11	0.06448249050156107
2212	12	0.209938226219478
2212	13	0.09843136277109465
2212	14	0.06427530166667594
2212	15	0.06725192301339433
2212	16	-0.1031195491119945
2212	17	0.03656708038055001
2212	18	0.043015864800480585
2212	19	0.0753398111303838
2212	20	-0.08229027568280856
2212	21	0.005377027813104007
2212	22	-0.5160947926941972
2212	23	0.07520048992271455
2212	24	0.0856933925698371
2212	25	0.061401982445104863
2213	1	-0.07441390861161779
2213	2	-0.8407576627403692
2213	3	-0.15261438260090543
2213	4	-0.03588581988196182
2213	5	0.0752778909846441
2213	6	-0.06900641851842693
2213	7	0.0025160464400002525
2213	8	0.1695295360137692
2213	9	-0.04977839451387922
2213	10	-1.4811835080218785
2213	11	-0.38900832634914195
2213	12	-0.016493753579624126
2213	13	-0.09528202819066678
2213	14	-0.08766181102351567
2213	15	-0.11502119046898684
2213	16	-0.07320440029749875
2213	17	-0.0744244946226118
2213	18	-0.1090450278831483
2213	19	-0.1532729953245635
2213	20	-0.007482775676173503
2213	21	-0.10136323490109334
2213	22	-0.021587176245958517
2213	23	-0.9074133721987536
2213	24	-1.649823066190649
2213	25	-1.436337175974721
2214	1	-0.42367286881649946
2214	2	-0.42575222917823297
2214	3	-0.23365244817162178
2214	4	-0.4243150674612082
2214	5	-0.16963141313816976
2214	6	-0.35814331233387975
2214	7	-0.17355440073762196
2214	8	-0.13385687664579393
2214	9	-0.4471766633300618
2214	10	-0.15970090145069804
2214	11	-0.26660122831599864
2214	12	-0.24778231513828786
2214	13	-0.22049172745202128
2214	14	-0.3134817164881625
2214	15	-0.4017085374223228
2214	16	-0.48085199111154964
2214	17	-0.53559368699689
2214	18	-0.2772861501262085
2214	19	-1.101638865729896
2214	20	-0.711036050391165
2214	21	-0.7885135134608134
2214	22	-0.9448610378942561
2214	23	-2.0344177787767777
2214	24	-1.581601382799768
2214	25	-1.90813520807243
2215	1	-0.698514207229703
2215	2	-0.4239929110698384
2215	3	-1.367028369286917
2215	4	-1.0905129420847715
2215	5	-0.47935898924600195
2215	6	-0.5387904610248455
2215	7	-0.5261428326474942
2215	8	-0.5011498672345951
2215	9	-0.6281365356701443
2215	10	-1.5400561013781626
2215	11	-0.75867040472383
2215	12	-0.8224323771548399
2215	13	-0.8797425595478074
2215	14	-0.9398215205528189
2215	15	-1.4004811906305008
2215	16	-0.9752194442602189
2215	17	-0.8103542036225235
2215	18	-0.8246922273964614
2215	19	-1.468760914661793
2215	20	-1.6502510788201903
2215	21	-1.0553029881643141
2215	22	-0.6708020587862222
2215	23	-1.0003666628749244
2215	24	-1.9568887980606817
2215	25	-1.1095914087155734
2216	1	-0.7688814694317045
2216	2	-0.7771877155040343
2216	3	-1.5106914157997942
2216	4	-1.0487863872152872
2216	5	-0.751290621578228
2216	6	-0.9991709470704535
2216	7	-0.6937720040149458
2216	8	-0.7605334348805215
2216	9	-0.6196806847248411
2216	10	-0.5266986652449717
2216	11	-0.6120177919688743
2216	12	-0.6450505660827446
2216	13	-1.5113366469969165
2216	14	-1.0006673841715283
2216	15	-1.4502885796017395
2216	16	-1.2072800652128137
2216	17	-2.273359477307203
2216	18	-1.23582072149879
2216	19	-1.1565957491633867
2216	20	-0.8801617243005282
2216	21	-0.7530251007382844
2216	22	-0.7295914121609641
2216	23	-0.6971684346850182
2216	24	-0.8055390897074233
2216	25	-0.6665297872777103
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2938.0
3	338.0
4	360.0
5	305.0
6	234.0
7	168.0
8	215.0
9	373.0
10	642.0
11	1327.0
12	2537.0
13	4815.0
14	10253.0
15	19659.0
16	27989.0
17	29062.0
18	27047.0
19	25069.0
20	24780.0
21	25034.0
22	24946.0
23	23452.0
24	23489.0
25	23256.0
26	22225.0
27	22636.0
28	24273.0
29	27004.0
30	32200.0
31	40565.0
32	55101.0
33	78961.0
34	115024.0
35	189325.0
36	360121.0
37	1166896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.76083851514146	17.70692366511333	9.719388859232755	23.812848960512458
2	9.00183438640701	28.122234780872148	30.799574241179606	32.076356591541234
3	23.94595605253664	35.139602960581044	23.754165685132683	17.16027530174963
4	13.627365123982873	25.941539102861423	23.46768964861015	36.96340612454555
5	8.498268892515593	46.18523900737477	33.77433346284536	11.542158637264277
6	32.31245675880473	27.13673277764966	19.91438243336738	20.636428030178237
7	19.830119906881396	38.13316388701401	21.78617889627357	20.250537309831028
8	29.862482644030347	36.09378546106229	21.436429680880817	12.607302214026546
9	28.420909985472427	21.58244696735353	16.60546577823434	33.39117726893971
10	18.198826921247026	37.9236695557682	28.026028688897686	15.851474834087087
11	22.727407511129964	33.377207974861314	12.955566629463828	30.939817884544894
12	24.9230748954597	40.11831083137597	23.286385440593143	11.672228832571188
13	31.314883382398556	23.846768224190505	16.824544500789013	28.01380389262193
14	20.335965970121713	36.43689579502995	21.347710693315936	21.879427541532397
15	41.74656523005062	20.613273381783827	17.21369435499713	20.42646703316842
16	13.687596267101569	29.54982002685183	37.76236131359806	19.000222392448542
17	29.86410524317283	34.28701687595675	24.039991285471242	11.808886595399173
18	30.425868586250726	23.77894635395173	27.810657711243618	17.98452734855393
19	22.845868913249937	38.29975017523976	28.42854212879031	10.425838782720001
20	24.46845269252644	29.661424946677446	24.43159330963076	21.438529051165354
21	15.268583035200598	34.341267020541	31.73955354837328	18.65059639588513
22	20.707110435863083	33.6492346418712	13.391244683554365	32.25241023871134
23	12.299985461350875	34.94209869724643	25.876406655488793	26.881509185913906
24	19.842984214389695	46.39120731372757	16.9435595023014	16.82224896958133
25	20.332092575346493	26.27520937533595	14.298359390349438	39.09433865896812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29116.0
1	29116.0
2	30747.5
3	32379.0
4	32379.0
5	32379.0
6	36949.5
7	41520.0
8	41520.0
9	41520.0
10	38569.0
11	35618.0
12	35618.0
13	35618.0
14	33735.0
15	31852.0
16	31852.0
17	31852.0
18	31165.0
19	30478.0
20	30478.0
21	30478.0
22	31080.5
23	31683.0
24	31683.0
25	31683.0
26	33708.5
27	35734.0
28	35734.0
29	35734.0
30	36214.0
31	36694.0
32	36694.0
33	36694.0
34	37625.0
35	38556.0
36	38556.0
37	38556.0
38	42585.5
39	46615.0
40	46615.0
41	46615.0
42	99779.5
43	152944.0
44	152944.0
45	152944.0
46	595587.0
47	1038230.0
48	1038230.0
49	1038230.0
50	842880.0
51	647530.0
52	647530.0
53	647530.0
54	402359.0
55	157188.0
56	157188.0
57	157188.0
58	89763.5
59	22339.0
60	22339.0
61	22339.0
62	17007.5
63	11676.0
64	11676.0
65	11676.0
66	9148.5
67	6621.0
68	6621.0
69	6621.0
70	4976.5
71	3332.0
72	3332.0
73	3332.0
74	2451.0
75	1570.0
76	1570.0
77	1570.0
78	1110.5
79	651.0
80	651.0
81	651.0
82	430.0
83	209.0
84	209.0
85	209.0
86	135.0
87	61.0
88	61.0
89	61.0
90	37.0
91	13.0
92	13.0
93	13.0
94	9.5
95	6.0
96	6.0
97	6.0
98	5.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27394343298313467
2	0.16500734393672006
3	0.18280708980732288
4	0.2032788529564227
5	0.228560247206817
6	0.18001174865443376
7	0.1775041632084597
8	0.19649603986485348
9	0.19822257410634384
10	0.2051287110723052
11	0.18453362404881324
12	0.1894254710663692
13	0.2013467789242787
14	0.19053538593589872
15	0.18235490226788495
16	0.184122544467506
17	0.18457473200694394
18	0.18880885169440836
19	0.18568464687647346
20	0.18383478876059095
21	0.18293041368171506
22	0.18663012991348008
23	0.1894254710663692
24	0.20410101211903714
25	0.1974004149437294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2432619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.491074458989962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18224060672274	7.992335586578168
2	10.55080943665847	2.2137865480519587
3	4.404797401701606	1.3863317255405119
4	2.3544776912797944	0.9880400308498842
5	1.4356140041361256	0.7530566705880409
6	0.9514998171971212	0.5989353257958202
7	0.6440219434116594	0.47295375130885975
8	0.4789807206859246	0.4020017924109367
9	0.344664633049644	0.3254312095833841
>10	2.1340778347511247	4.200089278263206
>50	0.24329321874553556	1.7882519966631785
>100	0.2020237710604379	4.393975300786606
>500	0.022403414300476578	1.6459521971593636
>1k	0.03222947320419437	7.4853982064516575
>5k	0.005109550629933254	3.4483063454629885
>10k+	0.013756482465204917	61.905154034505415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	230171	9.461859830906524	No Hit
CCCATGTACTCTGCGTTGATACCAC	221787	9.117210709938547	No Hit
GAGTACATGGGAAGCAGTGGTATCA	107864	4.43406879581225	No Hit
CATGTACTCTGCGTTGATACCACTG	85482	3.5139904769304198	No Hit
GCGTTGATACCACTGCTTCCCATGT	69234	2.8460683732224408	No Hit
TATCAACGCAGAGTACATGGGAAGC	59234	2.4349887919152158	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	58551	2.406912056511932	No Hit
ACTCTGCGTTGATACCACTGCTTCC	53280	2.190232009204894	No Hit
GGTATCAACGCAGAGTACATGGGAA	52763	2.1689791948513104	No Hit
GTATCAACGCAGAGTACATGGGAAG	52110	2.1421356981919484	No Hit
GCTTCCCATGTACTCTGCGTTGATA	39836	1.6375766200954611	No Hit
GTACTCTGCGTTGATACCACTGCTT	27226	1.1192052680670503	No Hit
GTGGTATCAACGCAGAGTACATGGG	25783	1.0598864844844178	No Hit
ATACCACTGCTTCCCATGTACTCTG	24821	1.0203406287626626	No Hit
CAGTGGTATCAACGCAGAGTACATG	23231	0.954978975334814	No Hit
ACATGGGAAGCAGTGGTATCAACGC	23225	0.9547323275860297	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	22011	0.9048272664153325	No Hit
GTTGATACCACTGCTTCCCATGTAC	21954	0.9024841128018815	No Hit
GCAGTGGTATCAACGCAGAGTACAT	21647	0.8898639696557495	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	21370	0.8784770652535394	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	21177	0.87054322933431	No Hit
TACCACTGCTTCCCATGTACTCTGC	21116	0.8680356438883361	No Hit
GATACCACTGCTTCCCATGTACTCT	20971	0.8620749899593814	No Hit
CATGGGAAGCAGTGGTATCAACGCA	20494	0.8424664939310267	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	18648	0.766581203221713	No Hit
CCACTGCTTCCCATGTACTCTGCGT	18412	0.7568797251028624	No Hit
CTTCCCATGTACTCTGCGTTGATAC	18356	0.754577679447542	No Hit
CCATGTACTCTGCGTTGATACCACT	17999	0.7399021383948741	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	16619	0.6831731561744769	No Hit
GTACATGGTAAGCAGTGGTATCAAC	16493	0.677993553450006	No Hit
TTCCCATGTACTCTGCGTTGATACC	15254	0.6270607933260407	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	14854	0.6106176100737518	No Hit
ACCATGTACTCTGCGTTGATACCAC	13532	0.5562728894249367	No Hit
TGATACCACTGCTTCCCATGTACTC	13051	0.5364999615640591	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	12744	0.5238798184179274	No Hit
ATCAACGCAGAGTACATGGGAAGCA	9261	0.3807008002486209	No Hit
CCCCATGTACTCTGCGTTGATACCA	7724	0.3175178686017005	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7075	0.2908388037748616	No Hit
TCCATGTACTCTGCGTTGATACCAC	7052	0.28989332073785495	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	6997	0.28763238304066524	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	6688	0.274930023978272	No Hit
GTACATGGGTAAGCAGTGGTATCAA	6685	0.2748067001038798	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5808	0.23875502082323619	No Hit
AAAAAGTACTCTGCGTTGATACCAC	5741	0.23600078762847776	No Hit
GCTTACCATGTACTCTGCGTTGATA	5460	0.22444945139374478	No Hit
CTGCGTTGATACCACTGCTTCCCAT	5090	0.20923950688537746	No Hit
CTCTGCGTTGATACCACTGCTTCCC	5035	0.20697856918818772	No Hit
GTACATGGAAAGCAGTGGTATCAAC	5011	0.20599197819305037	No Hit
TCCCATGTACTCTGCGTTGATACCA	4682	0.19246745996804268	No Hit
TCAACGCAGAGTACATGGGAAGCAG	4629	0.1902887381871144	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	4459	0.18330038530489154	No Hit
AAAGTACTCTGCGTTGATACCACTG	4366	0.17947734519873437	No Hit
ACTCTGCGTTGATACCACTGCTTAC	4223	0.17359890718604104	No Hit
GCGTTGATACCACTGCTTACCATGT	4190	0.17224234456772722	No Hit
AGTGGTATCAACGCAGAGTACATGG	4182	0.17191348090268144	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4089	0.16809044079652424	No Hit
GAGTACATGGAAAGCAGTGGTATCA	3802	0.15629245681300688	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	3744	0.153908195241425	No Hit
CTGCTTACCATGTACTCTGCGTTGA	3489	0.14342566591809075	No Hit
TATCAACGCAGAGTACATGGTAAGC	3451	0.1418635635091233	No Hit
TTGATACCACTGCTTCCCATGTACT	3400	0.13976705764445646	No Hit
AACGCAGAGTACATGGGAAGCAGTG	3381	0.13898600643997272	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	3365	0.13832827910988116	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3200	0.13154546601831196	No Hit
GGTATCAACGCAGAGTACATGGTAA	3179	0.13068219889756677	No Hit
ATGGTAAGCAGTGGTATCAACGCAG	3114	0.12801018161906982	No Hit
GTACATGGAAGCAGTGGTATCAACG	3073	0.1263247553357102	No Hit
GCTTTCCATGTACTCTGCGTTGATA	3070	0.12620143146131801	No Hit
ATGTACTCTGCGTTGATACCACTGC	3036	0.12480376088487347	No Hit
GTATCAACGCAGAGTACATGGTAAG	2932	0.12052853323927831	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2927	0.12032299344862471	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2827	0.11621219763555246	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	2682	0.1102515437065977	No Hit
CGTTGATACCACTGCTTCCCATGTA	2647	0.10881276517202242	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	2591	0.10651071951670195	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	2579	0.10601742401913329	No Hit
CAACGCAGAGTACATGGGAAGCAGT	2467	0.10141333270849237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.221591626144497E-5	0.0
2	0.0	0.0	0.0	8.221591626144497E-5	0.0
3	0.0	0.0	0.0	8.221591626144497E-5	0.0
4	0.0	0.0	0.0	8.221591626144497E-5	0.0
5	0.0	0.0	0.0	8.221591626144497E-5	0.0
6	0.0	0.0	0.0	8.221591626144497E-5	0.0
7	0.0	0.0	0.0	8.221591626144497E-5	0.0
8	0.0	0.0	0.0	8.221591626144497E-5	0.0
9	0.0	0.0	0.0	8.221591626144497E-5	0.0
10	0.0	0.0	0.0	2.466477487843349E-4	0.0
11	0.0	0.0	0.0	3.6997162317650234E-4	0.0
12	0.0	0.0	0.0	4.932954975686698E-4	0.0
13	0.0	0.0	0.0	4.932954975686698E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGCC	25	0.0060176714	19.008389	3
GTACAAA	920	0.0	17.056463	1
TATTCCA	40	2.753067E-4	16.631998	5
TACAAAA	1175	0.0	14.559618	2
TACATAA	590	0.0	14.497923	2
AGAACAA	1485	0.0	14.464296	2
GTACATA	675	0.0	14.371076	1
CGATGTA	40	0.0052647926	14.256291	2
GTAAAAA	430	0.0	13.933652	1
ATGTACG	145	0.0	13.765547	4
TGTACGC	145	0.0	13.764412	5
GTACGCT	145	0.0	13.756473	6
TACATGA	760	0.0	13.75607	2
CGGTGGT	155	0.0	13.480376	13
CGCAAAA	675	0.0	13.376273	2
ACATGAA	915	0.0	13.2954855	3
GTATCAT	115	3.8016879E-10	13.213696	18
TACGCTG	140	3.6379788E-12	12.891906	7
TATCTAC	75	1.4833402E-5	12.663385	19
GGTGGTA	170	0.0	12.290932	14
>>END_MODULE