FastQCFastQC Report
Wed 25 May 2016
SRR1295025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432619
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC24388910.025778800543776No Hit
GTACATGGGAAGCAGTGGTATCAAC2228409.1604973898502No Hit
GAGTACATGGGAAGCAGTGGTATCA1153284.740898595299963No Hit
CATGTACTCTGCGTTGATACCACTG943493.878494741675536No Hit
GCGTTGATACCACTGCTTCCCATGT743953.058226545135099No Hit
ACGCAGAGTACATGGGAAGCAGTGG722992.972064264893105No Hit
TATCAACGCAGAGTACATGGGAAGC644082.6476813672835737No Hit
ACTCTGCGTTGATACCACTGCTTCC591302.4307135642696207No Hit
GGTATCAACGCAGAGTACATGGGAA568062.3351786695738213No Hit
GTATCAACGCAGAGTACATGGGAAG551962.2689948569833582No Hit
GCTTCCCATGTACTCTGCGTTGATA438191.8013096173301286No Hit
ACATGGGAAGCAGTGGTATCAACGC298761.228141357113465No Hit
CAGTGGTATCAACGCAGAGTACATG281311.1564079701753542No Hit
GTGGTATCAACGCAGAGTACATGGG276981.1386082243047513No Hit
CATGGGAAGCAGTGGTATCAACGCA268481.1036664598936372No Hit
GTACTCTGCGTTGATACCACTGCTT260331.0701634740170984No Hit
ATACCACTGCTTCCCATGTACTCTG257591.0588998934892804No Hit
GGGAAGCAGTGGTATCAACGCAGAG252901.0396202611259717No Hit
GCAGTGGTATCAACGCAGAGTACAT245321.008460428862884No Hit
GATACCACTGCTTCCCATGTACTCT230090.9458530086297937No Hit
CTGCTTCCCATGTACTCTGCGTTGA227380.9347127519763678No Hit
TACCACTGCTTCCCATGTACTCTGC220210.9052383459966399No Hit
GCAGAGTACATGGGAAGCAGTGGTA219790.9035118117551495No Hit
GTTGATACCACTGCTTCCCATGTAC215470.8857531738426773No Hit
CCATGTACTCTGCGTTGATACCACT214070.8799980597043763No Hit
CTTCCCATGTACTCTGCGTTGATAC206880.8504414378083868No Hit
CCACTGCTTCCCATGTACTCTGCGT204400.8402466641919676No Hit
GTACTTTTTTTTTTTTTTTTTTTTT200680.8249545037673387No Hit
AAGCAGTGGTATCAACGCAGAGTAC193050.7935891317135976No Hit
GTACATGGTAAGCAGTGGTATCAAC175520.721526881110441No Hit
ATGGGAAGCAGTGGTATCAACGCAG174530.7174571932554995No Hit
TTCCCATGTACTCTGCGTTGATACC150010.616660479918968No Hit
ACCATGTACTCTGCGTTGATACCAC147010.6043280924797513No Hit
GTATCAACGCAGAGTACTTTTTTTT145310.5973397395975284No Hit
TATCAACGCAGAGTACTTTTTTTTT144900.5956543133141687No Hit
GGTATCAACGCAGAGTACTTTTTTT135210.5558207018854987No Hit
TGATACCACTGCTTCCCATGTACTC130160.5350611830294839No Hit
ATCAACGCAGAGTACATGGGAAGCA108730.44696682875534555No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97780.40195361460220447No Hit
GAGTACTTTTTTTTTTTTTTTTTTT95950.39443085826428226No Hit
CCCCATGTACTCTGCGTTGATACCA89020.36594304327969157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA88930.36557307165651504No Hit
GGTAAGCAGTGGTATCAACGCAGAG83190.34197710368948037No Hit
GGAAGCAGTGGTATCAACGCAGAGT81630.33556426222108765No Hit
GTACATGGGTAAGCAGTGGTATCAA72010.2960184064993326No Hit
TCCATGTACTCTGCGTTGATACCAC71430.2936341449277507No Hit
GAGTACATGGTAAGCAGTGGTATCA65970.27118919978837625No Hit
CTGCGTTGATACCACTGCTTCCCAT60370.24816874323517163No Hit
AAAAAGTACTCTGCGTTGATACCAC60100.24705882836564214No Hit
GCTTACCATGTACTCTGCGTTGATA57820.2376862139118374No Hit
CTCTGCGTTGATACCACTGCTTCCC56620.23275325893615073No Hit
TCAACGCAGAGTACATGGGAAGCAG55560.2283958153742941No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA53270.21898209296235868No Hit
TGGGAAGCAGTGGTATCAACGCAGA51180.21039052971303768No Hit
GTACATGGAAAGCAGTGGTATCAAC50580.2079240522251943No Hit
TCCCATGTACTCTGCGTTGATACCA49080.20175785850558595No Hit
ACTCTGCGTTGATACCACTGCTTAC47560.19550944886971613No Hit
AAAGTACTCTGCGTTGATACCACTG47410.1948928294977553No Hit
AGTGGTATCAACGCAGAGTACATGG47400.19485172153962457No Hit
ACGCAGAGTACATGGTAAGCAGTGG44190.18165606697966266No Hit
GCGTTGATACCACTGCTTACCATGT42810.17598316875762296No Hit
AACGCAGAGTACATGGGAAGCAGTG41760.17166683315389708No Hit
GAGTACATGGAAAGCAGTGGTATCA40960.1683781965034393No Hit
TATCAACGCAGAGTACATGGTAAGC38640.15884115021711168No Hit
CTGCTTACCATGTACTCTGCGTTGA37020.15218166099993463No Hit
ATGGTAAGCAGTGGTATCAACGCAG36870.1515650416279738No Hit
ACTGCTTCCCATGTACTCTGCGTTG36300.14922188801452263No Hit
GGTATCAACGCAGAGTACATGGTAA33510.13775276769605105No Hit
GCTTTCCATGTACTCTGCGTTGATA33480.13762944382165887No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32550.13380640371550168No Hit
TTGATACCACTGCTTCCCATGTACT32470.1334775400504559No Hit
CAACGCAGAGTACATGGGAAGCAGT32370.1330664604691487No Hit
CGTTGATACCACTGCTTCCCATGTA31910.13117549439513543No Hit
GTATCAACGCAGAGTACATGGTAAG31690.13027111931625956No Hit
GTACATGGAAGCAGTGGTATCAACG30710.12624253941944877No Hit
CGCAGAGTACATGGGAAGCAGTGGT29770.12237839135516082No Hit
ATGTACTCTGCGTTGATACCACTGC29440.121021828736847No Hit
GTGGTATCAACGCAGAGTACTTTTT29060.11945972632787955No Hit
ACGCAGAGTACATGGAAAGCAGTGG27670.11374572014770912No Hit
GCGTTGATACCACTGCTTTCCATGT24500.10071449742027008No Hit
GAACAAAAAAAAAAAAAAAAAAAAA24380.10022120192270143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA307.726863E-418.9991153
GGACGCA1750.017.9130848
GTACAAA4500.017.119261
AACGCCG453.5422523E-516.8877566
GGGACGC1900.016.4988927
CAGCGTC350.002171349216.2849562
GCAACCC350.002171349216.2849563
CGGAATC350.002171647316.2846216
GCCTGTG350.002171647316.284627
TCTGTCG350.002171647316.284628
TGGGACG2000.015.6739486
CGCAAAA2500.015.5792742
TACAAAA4800.015.23887352
TTTACTC1004.0017767E-1115.1992923
CGAGTAC508.722159E-515.1989811
GCCGAGT508.722159E-515.198989
GACGCAG2050.014.8282739
GTACAAG2250.014.7944231
ACGCAGT2200.014.68083310
ACCAGGA602.5705664E-514.2493362
Produced by FastQC (version 0.11.5)