FastQCFastQC Report
Wed 25 May 2016
SRR1295024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402882
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC284510.4443467800905905No Hit
CCCATGTACTCTGCGTTGATACCAC269070.42023263898975494No Hit
GAGTACATGGGAAGCAGTGGTATCA128130.2001130116094596No Hit
CATGTACTCTGCGTTGATACCACTG100930.15763214127638148No Hit
GCGTTGATACCACTGCTTCCCATGT85990.13429889852725696No Hit
GTATCAACGCAGAGTACTTTTTTTT80590.12586519632877818No Hit
GTATCAACGCAGAGTACATGGGAAG69760.10895093803071805No Hit
ACGCAGAGTACATGGGAAGCAGTGG68800.10745161319543294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN66340.10360959330501483No Hit
TATCAACGCAGAGTACATGGGAAGC64670.1010013928103001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC1103.8025973E-812.095273
TCGTCCG1909.094947E-1210.4991839
CCTAGAC6200.010.4231133
TTGCGTG2400.010.28933516
CGTCCGC2151.8189894E-1210.16152210
TAGGACC4100.09.9681014
TAAGACA8700.09.9414564
GTCTAGG5750.09.7567661
TAGACAG10100.09.504175
TACGTCG1201.6936476E-59.504175
CGGACGT800.00452335049.49881510
GTCCCGC2550.09.3222681
GTACCGC1856.730261E-99.24186
ATGCGAA1151.1147596E-49.08468411
TACGACT950.00181728359.0042334
ATCGTCC2850.08.999378
CTAGGAC6350.08.9796153
GTCCTAA6150.08.96756651
CGCATCG2356.548362E-118.8975215
ATAGGAC3650.08.8525073
Produced by FastQC (version 0.11.5)