FastQCFastQC Report
Wed 25 May 2016
SRR1295024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402882
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC295630.46171395943264304No Hit
GTACATGGGAAGCAGTGGTATCAAC279300.4362098192657619No Hit
GTATCAACGCAGAGTACTTTTTTTT214810.33548954986207774No Hit
GGTATCAACGCAGAGTACTTTTTTT185820.29021306342987424No Hit
TATCAACGCAGAGTACTTTTTTTTT147510.23038063172177778No Hit
GAGTACATGGGAAGCAGTGGTATCA137500.21474704672052367No Hit
CATGTACTCTGCGTTGATACCACTG113110.1766548251240613No Hit
GCGTTGATACCACTGCTTCCCATGT91390.14273260072573568No Hit
ACGCAGAGTACTTTTTTTTTTTTTT84210.1315189003951658No Hit
ACGCAGAGTACATGGGAAGCAGTGG82790.12930114907630658No Hit
GTATCAACGCAGAGTACATGGGAAG74010.11558857402026151No Hit
TATCAACGCAGAGTACATGGGAAGC70200.10963812858022372No Hit
ACTCTGCGTTGATACCACTGCTTCC67210.10496835643699198No Hit
GCTTCCCATGTACTCTGCGTTGATA66680.10414060418417832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG551.9629837E-413.817089
ATCGCTA550.003072078812.08994511
TGGACCG1856.002665E-1110.2694515
CTACACT11100.09.7559794
GTCTAGG6250.09.7417551
TAGACAG9200.09.4992435
ACCGATC900.00111532579.4992438
ACCGTCC1606.656228E-89.4992428
CGGGACT2357.2759576E-129.2971314
TACACTG16400.09.2675535
GGACCGA1356.8631616E-69.1474196
GTCTACG1252.7116717E-59.13289451
AGACCGT3350.09.0739036
CTAGACA7400.08.985774
GTCCTAT5300.08.97493651
TCGTCTA2553.6379788E-128.94046314
GTATAGG6500.08.781631
GTACCGT1304.3775734E-58.7685326
CTTAGAC5100.08.7542043
GACCGAT1107.155676E-48.6356747
Produced by FastQC (version 0.11.5)